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BRCA1 Gene Q356R (1186A=G) Polymorphism and Epithelial Ovarian Cancer Incidence Rakhmatsyah, Benyamin; Saleh, Agustria Z; Sanif, Rizal; Yuwono, Yuwono
Indonesian Journal of Obstetrics and Gynecology Volume. 3, No. 4, October 2015
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To determine the association between BRCA1 gene Q356R (1186A=G) polymorphism and epithelial ovarian cancer incidence. Methods: This study is an observational analytic study with casecontrol study design. All patients diagnosed with epithelial ovarian cancer that were treated in the outpatient clinic and inpatient ward of the Department of Obstetrics and Gynecology, Dr. Mohammad Hoesin Hospital, Palembang who met the inclusion criteria were included in this study. DNA extraction was performed on blood samples, followed by PCR-RFLP process. Results: We obtained the genotype distribution of BRCA1 Q356R (1186A=G) polymorphisms to be QQ genotype (wild-type) on all 50 subjects in the case group (100%) and 50 control subjects (100%). Similarly, all BRCA1 alleles have the Q allele. The results of this study found no polymorphism of the BRCA1 Q356R (1186A=G) in the ovarian cancer and control groups. Conclusion: Polymorphism of BRCA1 gene Q356R (1186A=G) was not significantly associated with epithelial ovarian cancer incidence. [Indones J Obstet Gynecol 2015; 3-4: 206-211] Keywords: BRCA1 gene, epithelial ovarian cancer, polymorphism

p53 Gene Codon 72 Polymorphisms among Cervical Carcinoma Patients Basyar, Rustham; Saleh, Agustria Z; Sastradinata, Irawan; Yuwono, Yuwono
Indonesian Journal of Obstetrics and Gynecology Volume. 3, No. 3, July 2015
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To identify the association between p53 gene codon 72 polymorphism and cervical carcinoma. Method: An analytic observational study with case-control design, from November 2013 until March 2014 in the Department of Obstetrics and Gynecology and Microbiology Laboratory Faculty of Medicine, Sriwijaya University, Dr. Moh. Hoesin Hospital Palembang. Result: In total there are 100 samples analyzed consisting of 50 subjects and 50 control groups. Genotype distribution in subject group are 54% Arg/Arg, 42% Pro/Arg and 4% Pro/Pro, and in control group are 36% Arg/Arg, 46% Pro/Arg and 18% Pro/Pro. Arg/Arg genotype is at risk of cervical carcinoma 6.7 times higher compared with Pro/Pro genotype (p=0.013; OR 6.75; 95% CI 1.34-34.94). Arg allele in the p53 gene codon 72 increase the risk of cervical carcinoma 2.6 times more than Pro allele. Conclusion: Proline mutation to Arginine in gene p53 P72R is one of the risk factor for cervical carcinoma. Keywords: arginine, cervical carcinoma, gene p53 codon 72, polymorphism, proline

p53 Gene Codon 72 Polymorphisms among Cervical Carcinoma Patients Basyar, Rustham; Saleh, Agustria Z; Sastradinata, Irawan; Yuwono, Yuwono
Indonesian Journal of Obstetrics and Gynecology Volume. 3, No. 3, July 2015
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To identify the association between p53 gene codon 72 polymorphism and cervical carcinoma. Method: An analytic observational study with case-control design, from November 2013 until March 2014 in the Department of Obstetrics and Gynecology and Microbiology Laboratory Faculty of Medicine, Sriwijaya University, Dr. Moh. Hoesin Hospital Palembang. Result: In total there are 100 samples analyzed consisting of 50 subjects and 50 control groups. Genotype distribution in subject group are 54% Arg/Arg, 42% Pro/Arg and 4% Pro/Pro, and in control group are 36% Arg/Arg, 46% Pro/Arg and 18% Pro/Pro. Arg/Arg genotype is at risk of cervical carcinoma 6.7 times higher compared with Pro/Pro genotype (p=0.013; OR 6.75; 95% CI 1.34-34.94). Arg allele in the p53 gene codon 72 increase the risk of cervical carcinoma 2.6 times more than Pro allele. Conclusion: Proline mutation to Arginine in gene p53 P72R is one of the risk factor for cervical carcinoma. Keywords: arginine, cervical carcinoma, gene p53 codon 72, polymorphism, proline

BRCA1 Gene Q356R (1186A=G) Polymorphism and Epithelial Ovarian Cancer Incidence Rakhmatsyah, Benyamin; Saleh, Agustria Z; Sanif, Rizal; Yuwono, Yuwono
Indonesian Journal of Obstetrics and Gynecology Volume. 3, No. 4, October 2015
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To determine the association between BRCA1 gene Q356R (1186A=G) polymorphism and epithelial ovarian cancer incidence. Methods: This study is an observational analytic study with casecontrol study design. All patients diagnosed with epithelial ovarian cancer that were treated in the outpatient clinic and inpatient ward of the Department of Obstetrics and Gynecology, Dr. Mohammad Hoesin Hospital, Palembang who met the inclusion criteria were included in this study. DNA extraction was performed on blood samples, followed by PCR-RFLP process. Results: We obtained the genotype distribution of BRCA1 Q356R (1186A=G) polymorphisms to be QQ genotype (wild-type) on all 50 subjects in the case group (100%) and 50 control subjects (100%). Similarly, all BRCA1 alleles have the Q allele. The results of this study found no polymorphism of the BRCA1 Q356R (1186A=G) in the ovarian cancer and control groups. Conclusion: Polymorphism of BRCA1 gene Q356R (1186A=G) was not significantly associated with epithelial ovarian cancer incidence. [Indones J Obstet Gynecol 2015; 3-4: 206-211] Keywords: BRCA1 gene, epithelial ovarian cancer, polymorphism

COMPARISON BETWEEN BELUNTAS (PLUCHEA INDICA LEES) FRACTION AND KETOCONAZOLE INVITRO ON CANDIDA ALBICANS Marsasi, Bina; Yuwono, Yuwono; Salni, Salni
Biomedical Journal of Indonesia Vol 5, No 1 (2019)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v5i1.7974

Beluntas leaves are useful as a treatment for flour albus and discharge caused by fungi. This study aims to find the anti-fungal activity of fraction of beluntas (Pluchea indica Lees) leaf extract against Candida albicans. This research is an in vitro experimental study using the Posttest-only Control Group Design study design. Methanol extract is obtained through maceration process while the fraction is through liquid-liquid fractionation. The most active fraction is determined by the antifungal activity test. The most active fractions with concentrations of 10%, 5%, 2.5%, 1.25%, 0.625% and the diameter of the inhibition zone 0.3125% were measured by the liquid dilution method. Equivalence with drugs is assessed through regression analysis, then the class of active compounds is determined through bioautographic testing using thin layer chromatography. The research results obtained methanol-fraction-water can inhibit the growth of Candida albicans while the methanol extract, n-hexane and ethyl acetate do not inhibit. The methanol fraction of water which has inhibition zone diameters starts at a concentration of 0.3125% with an average diameter of 7.34 mm and at a concentration of 10% the largest diameter of 17.10 mm is obtained. Through the diluent liquid and the KHM solid fraction from the methanol-water fraction is inhibited at a concentration of 0.3125%. Equivalence test shows that 1 mg / ml of ketoconazole is equivalent to 39,561 mg / ml of methanol-water fraction of beluntas leaves. Compounds that inhibit fungi are thought to be active groups of flavonoid and alkaloid compounds. The methanol-water fraction of beluntas leaves (Pluchea indica Lees) has antifungal activity against Candida albicans in vitro.

RELATIONSHIP OF SNP (RS867500) STX1A EXON 10 GENE WITH INTELLECTUAL INTELLIGENCE LEVEL Qur'andini, Dalinur; Yuwono, Yuwono; Triwani, Triwani
Biomedical Journal of Indonesia Vol 5, No 1 (2019)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v5i1.7975

The purpose of this study was to determine the relationship of SNP (rs867500) on the STX1A Exon 10 gene with the level of intellectual intelligence. This study was an observational analytic study with a cross sectional study design with a population of 368 and a sample of 36 which was divided into two groups, namely the above average IQ group and the average IQ group. To find out the relationship of SNP (rs867500) STX1A Exon 10 gene with intelligence level used X2 test (Chi-square). The results showed no SNPs were found in the STX1A exon 10 gene. The STX1A gene can be said to contribute to intelligence along with other genes, namely IGFR2 and FNBPL1 genes, although there is no polymorphism in the exon 10 STX1A gene.

Identifikasi Gen SHV pada Enterobacteriaceae Produsen Extended-Spectrum Beta-Lactamases (ESBLs) Yuwono, Yuwono
Syifa'Medika Vol 4, No 1 (2013): Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan
Publisher : Faculty of Medicine

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/sm.v4i1.1413

Salah satu problem resistensi terhadap antimikroba terutama di rumah sakit adalah kelompokEnterobacteriaceae yang memproduksi Extended-Spectrum Beta-Lactamases (ESBLs). Tujuanpenelitian ini adalah untuk mengidentifikasi gen SHV pada Enterobacteriaceae produsen ESBLs padapasien yang dirawat di RSUP Mohammad Hoesin Palembang. Didapatkan 75 sampelEnterobacteriaceae berdasarkan uji Double Disk Approximation dan PCR. Spesies yang ditemukanadalah Klebsiella pneumoniae 30 (40,00%), Escherichia coli 17 (22,66%), Enterobacter sp 14(18,67%), Proteus Sp 12 (16,00%), dan Acinetobacter calcoaceticus 2 (2,67%). Berdasarkan hasilPCR didapatkan 32 sampel mengandung gen SHV. Distribusi gen SHV adalah 20 (62,50%) padaKlebsiella pneumoniae, 7 (21,87%) pada Escherichia coli, 4 (12,50%) pada Enterobacter sp dan 1(3,13%) pada Proteus sp. Hasil penelitian ini menunjukkan bahwa Klebsiella pneumoniae dominanpada Enterobacteriaceae produsen ESBLs dan gen SHV juga dominan ditemukan pada spesies ini.

Mekanisme Molekuler Resistensi Methicillin Resistens Staphylococcus aureus (MRSA) Yuwono, Yuwono
Syifa'Medika Vol 2, No 1 (2011): Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan
Publisher : Faculty of Medicine

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/sm.v2i1.2855

MRSA

Polymerase Chain Reaction (PCR) sebagai Baku Emas Identifikasi Methicillin Resistant Staphylococcus aureus (MRSA) Yuwono, Yuwono
Syifa'Medika Vol 3, No 1 (2012): Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan
Publisher : Faculty of Medicine

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/sm.v3i1.2866

PCR MRSA

Methicillin Resistant Staphylococcus aureus (MRSA): Ancaman Serius pada Penatalaksanaan Pasien Infeksi Yuwono, Yuwono
Syifa'Medika Vol 1, No 2 (2011): Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan
Publisher : Faculty of Medicine

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/sm.v1i2.2851

MRSA

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