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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Ecotrophic, Journal of Environmental Science Journal of Science and Applicative Technology Chimica et Natura Acta ISM (Intisari Sains Medis) : Jurnal Kedokteran Jurnal Media Sains Jurnal Ilmu Pendidikan Indonesia

N. W. Bogoriani

Program Studi Kimia FMIPA Universitas Udayana, Jimbaran, Bali, Indonesia

Author-ID : 4423114

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics

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Asupan Glikosida Flavonoid Terong Belanda (Solanum betaceum Cav.) Terhadap Aktivitas Superoksida Dismutase Dan Kadar Malondialdehid Tikus Wistar Yang Diberi Aktivitas Fisik Maksimal Jeane, Magda; Astiti Asih, Ida Ayu Raka; Bogoriani, Ni Wayan
Jurnal Media Sains Vol 2, No 1 (2018): JURNAL MEDIA SAINS
Publisher : LPPM Universitas Dhyana Pura

ABSTRAKTujuan dari penelitian ini adalah untuk mengetahui pengaruh asupan glikosida flavonoid terong belanda (Solanum betaceum Cav.) terhadap aktivitas Superoksida Dismutase (SOD) darah tikus Wistar yang diberi aktivitas fisik maksimal. Sampel penelitian adalah 24 ekor tikus yang dibagi kedalam 4 kelompok perlakuan yaitu: kontrol negatif, kontrol positif, pemberian ekstrak etanol, dan pemberian fraksi n-butanol. Aktivitas SOD diukur menggunakan kit Biovision. Hasil penelitian menunjukkan bahwa perlakuan yang diberikan memberikan perbedaan yang signifikan (p<0,05) terhadap aktivitas SOD. Pemberian glikosida flavonoid fraksi n-butanol meningkatkan aktivitas SOD tikus Wistar yang diberikan aktivitas fisik maksimal sebanyak 22,79% lebih tinggi dibandingkan pemberian asupan ekstrak etanol terong belanda. Keyword : Solanum betaceum Cav., Superoksida Dismutase, Aktivitas fisik, stres oksidatifABSTRACTThis study was conducted to determine the effect of flavonoid glycoside of Tamarillo (Solanum betaceum Cav.) on the activity of Wistar rat?s blood Superoxide Dismutase (SOD) enzyme provided maximum physical activity. Twenty four rats divided into 4 groups; negative control, positive control, ethanol extract, and n-butanol fraction. Superoxide Dismutase Enzyme activity was measured using Biovision kit. Results showed that the treatments gave significant difference (p<0.05) to SOD. Flavonoid glycoside from n-butanol fraction increased SOD activity of Wistar rat 22,79% higher than ethanol extract. Keyword : Solanum betaceum Cav., Superoxide Dismutase, Physical activity, oxidative stress

Potensi Ekstrak Metanol Daun Andong Merah (Cordyline Fruticosa (L.) A Chev.) dalam Menurunkan Kadar Asam Lemak Bebas dan Glukosa Darah pada Tikus Obesitas Mahayani, Putu Ayu Erna; Bogoriani, Ni Wayan; Bawa Putra, Anak Agung
Jurnal Media Sains Vol 3, No 1 (2019): JURNAL MEDIA SAINS
Publisher : LPPM Universitas Dhyana Pura

ABSTRAKTelah dilakukan uji aktivitas ekstrak metanol daun andong merah (Cordyline fruticosa (L.) A Chev.) dalam menurunkan kadar glukosa darah dan asam lemak bebas pada tikus wistar betina yang obesitas. Hasil uji fitokimia menunjukkan bahwa ekstrak mengandung senyawa polifenol, flavonoid, saponin, alkaloid, dan steroid. Hasil uji kadar glukosa dan asam lemak bebas menunjukkan bahwa pemberian ekstrak dosis 35mg/kg BB dan 70mg/kg BB dapat menurunkan kadar glukosa darah dan asam lemak bebas dibandingkan dengan kelompok tikus yang hanya diberi pakan tinggi lemak dengan perbedaan nyata p<0,05.Kata kunci: asam lemak bebas, Cordyline fruticosa (L.) A Chev., glukosa darah, obesitasABSTRACTThe activity of methanol extract of red andong leaf (Cordyline fruticosa (L.) A Chev.) to decrease blood glucose level and free fatty acid were investigated in obese female wistar rats. The results of phytochemical tests showed that the extract contains polyphenolic, flavonoid, saponin, alkaloid, and steroid compounds. The results of glucose and free fatty acid test showed that giving of extract with dose 35mg / kg BW and 70mg / kg BW could decrease blood glucose level and free fatty acid compared to the group of wistar rats were fed high fat with significant difference p <0,05.Keywords: blood glucose, Cordyline fruticosa (L.) A Chev., free fatty acid, obesity

Identifikasi Dan Karakterisasi Profil Asam Lemak Virgin Coconut Oil Dengan Penambahan Ekstrak Etanol Kunyit Putih (Curcuma zedoaria Rosc.) Ni Pt. Pande Mirah Surya Dewi; Ni Wayan Bogoriani; Ni Made Suaniti
Chimica et Natura Acta Vol 7, No 3 (2019)
Publisher : Departemen Kimia

Hasil perkebunan kelapa di Provinsi Bali cukup banyak namun pemasarannya dalam bentuk produk diversifikasi masih belum maksimal, contohnya virgin coconut oil (VCO). Virgin coconut oil mempunyai harga jual tinggi serta berbagai manfaat di bidang kesehatan maupun kosmetik. Tujuan penelitian ini adalah mengetahui kadar bilangan peroksida, asam lemak bebas, serta kapasitas antioksidan VCO dengan penambahan ekstrak etanol kunyit putih serta mengetahui profil asam lemak bebas VCO dengan penambahan ekstrak etanol kunyit putih. Metode penelitian ini adalah rancangan the pretest – posttest control group design dengan perlakuan 3 konsentrasi ekstrak etanol kunyit putih yaitu 5%, 10% dan 15% serta diukur pada awal, 1 minggu, 2 minggu, dan 4 minggu. Hasil penelitian menunjukkan kadar bilangan peroksida tidak terdeteksi pada semua sampel. Kadar asam lemak bebas VCO dengan penambahan ekstrak etanol kunyit putih berkisar pada (0,16 – 0,21%). Kapasitas antioksidan VCO dengan penambahan ekstrak etanol kunyit putih berkisar pada (0,94 – 13,83 mg/L GAEAC). Profil asam lemak VCO dengan penambahan ekstrak etanol kunyit putih adalah asam kaproat, asam kaprilat, asam kaprat, asam laurat, asam miristat, asam stearate, dan asam palmitat.

SUHU DAN WAKTU OPTIMUM PROSES EKSTRAKSI ANTOSIANIN DALAM UBI JALAR UNGU (Ipomoea batatas L.) DENGAN ?-L-ARABINOFURANOSIDASE I N. Wirajana; N. M. T. Juliasari; A.A. I.A.M. Laksmiwati; N. W. Bogoriani
Jurnal Kimia (Journal of Chemistry) Vol.13 No.1 Januari 2019
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Enzyme-assisted extraction (EAE) method is one of the most environmentally friendly methods of enzyme application in the extraction of bioactive compounds. The purpose of this study was to determine the optimum temperature and time required in the extraction of anthocyanin compounds from purple sweet potato (Ipomoea batatas L.) with and without ?-L-arabinofuranosidase (AbfA) - assisted. The AbfA enzyme was obtained from Saccharomyces cerevisiae recombinant strain BJ1824 contain pYHMI-Af plasmid. The optimum temperature and time in the extraction of anthocyanin compound with and without ?-L-arabinofuranosidase from purple sweet potato were performed on the 40, 50, 60 and 700C; and 150, 180, 210 minutes. The extraction was done by ethanol solvent of 60,32% (v/v) acidified with citric acid of 2,39% (b/v). The measurement of anthocyanin levels using UV-Vis Spectrophotometer at 527 nm and 700 nm wavelengths at pH 1,0 and 4,5. The optimum condition of non-enzyme-assisted extraction was at 600C for 210 minutes, with the anthocyanin levels of 26,3842 mg/L; while with the AbfA enzyme-assisted at 500C for 180 minutes, with the anthocyanin levels of 28,2056 mg/L. The extraction with enzyme-assisted resulted the anthocyanin levels of 6,90% higher than without the using of enzyme.

AKTIVITAS EKSTRAK METANOL RIMPANG KUNYIT PUTIH (Curcuma zedoaria Rosc.) SEBAGAI HIPOLIPIDEMIA PADA TIKUS WISTAR PUTIH OBESITAS DENGAN DIET TINGGI KOLESTEROL A. Intan Saridewi; N.W. Bogoriani; P. Suarya
Jurnal Kimia (Journal of Chemistry) Vol.12 No.2 Juli 2018
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Obesitas merupakan keadaan akumulasi lemak yang tidak normal. Obesitas berdampak pada berbagai macam penyakit degeneratif yaitu diabetes militus, hiperkolesterolimia, aterosklerosis, dislipidemia, dan penyakit jantung koroner. Kunyit putih (Curcuma zedoaria Rosc.) diketahui mengandung senyawa polifenol (kurkuminoid) yang dapat berperan sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak rimpang kunyit putih pada profil lipid tikus wistar obesitas dengan diet tinggi kolesterol serta mengetahui golongan senyawa metabolit sekunder yang terkandung dengan uji fitokimia. Uji Aktivitas ekstrak rimpang kunyit putih menggunakan tikus wistar betina obesitas yang diberi diet tinggi kolesterol sebagai kontrol positif dan pemberian ekstrak dengan dosis 35 dan 70 mg/kgBB/hari sebagai perlakuan serta tikus wistar betina normal yang diberi diet standar sebagai kontrol negatif. Hasil analisis menunjukkan bahwa ekstrak metanol rimpang kunyit putih pada dosis 35 dan 70 mg/kgBB/hari mampu menurunkan kadar trigliserida, kolesterol LDL, dan kolesterol total dan pada dosis 70 mg/kgBB/hari dapat meningkatkan kadar kolesterol HDL secara signifikan (p<0,05). Hal ini disebabkan oleh adanya golongan senyawa flavonoid, alkaloid, fenolat, terpenoid dan saponin yang positif terkandung dalam ekstrak rimpang kunyit putih dari uji fitokimia. Berdasarkan hal tersebut, ekstrak rimpang kunyit putih pada dosis 70 mg/kgBB/hari mampu berperan sebagai hipolipidemia pada tikus wistar obesitas dengan diet tinggi kolesterol. Kata kunci : ekstrak metanol rimpang kunyit putih, trigliserida, kolesterol LDL, kolesterol HDL,

EFEK BERBAGAI MINYAK PADA METABOLISME KOLESTEROL TERHADAP TIKUS WISTAR Ni Wayan Bogoriani; Ketut Ratnayani
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

This study was conducted over 10 weeks using several types of oils containing unsaturated fatty acids, saturated fatty acids and trans fatty acids, namely coconut oil, bulk oil, used cooking oil and pig fat. Animal tests were used to determine the effects of various oils on the metabolism of cholesterol in wistar rats. The study design was a randomized post-test only control group design, conducted in mice. Twenty-five rats were divided into five groups: control, treatment 1, treatment 2, treatment 3 and treatment 4, each of 5 rats. The control group was given standard diet only, treatment- 1 standard food and coconut oil 2.5 ml / day, treatment-2 standard food and bulk oil 2.5 ml / day, treatment-3 standard food and used cooking oil 2.5 ml / day and the treatment-4 standard food and pig fat 2.5 ml / day. After 10 weeks of treatment their blood plasm samples were taken for examination for total cholesterol, LDL, HDL, triglycerides, VLDL cholesterol, and the ratio of total / HDL. The results showed an increase in total cholesterol, LDL, triglycerides, VLDL, the ratio of total cholesterol / HDL and a decrease in blood plasm HDL cholesterol significantly (p <0.05) in the treatment of waste cooking oil compared with the control and other treatments. Based on these results it can be concluded that the used cooking oil has the potential to cause oxidative stress and inflamation which is a risk factor for atherosclerosis formation.

PERBANDINGAN MASSA OPTIMUM CAMPURAN PEWARNA ALAMI PADA KAYU JENIS AKASIA (Acacia leucopholea) N. W. Bogoriani; A. A. Bawa Putra
Jurnal Kimia (Journal of Chemistry) Vol. 3, No. 1 Januari 2009
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (63.375 KB)

Wood is one of several materials that is very useful for man. Now adays wood with low quality tends to beused more. The high demand causes good quality wood getting harder to find. To overcome this problem, low qualitywood like acacia was dyed using a mixture of natural dye. The dye was prepared from urcaria gambir-piper betleLinn leaves, areca catechw seed, and 0.5 % w/v of CaCO3 in water. The physical and chemical properties of thedyed wood was tested using aluminium sulphate solution and detergent solution.The optimum mass for the dye was 5.3 uncaria : 3.0 piper; 3.0 areca. After 30 minutes soaking in themixture, reddish brown coolour was produced, with maximum adsorption of 0.23 g in wood surface that showeddurability of the dye highest from dye appearance and mixture mass that be lost.

itu PRODUKSI HIDROLISAT PROTEIN ANTIOKSIDAN MELALUI HIDROLISIS ENZIMATIK PROTEIN KULIT AYAM BROILER DENGAN ENZIM PAPAIN N. M. Puspawati; P. P. Dewi; N. W. Bogoriani; N. K. Ariati
Jurnal Kimia (Journal of Chemistry) Vol. 14, No. 2 Juli 2020
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2020.v14.i02.p16

Penelitian ini bertujuan untuk menentukan aktivitas antioksidan hidrolisat protein yang dihasilkan dari proses hidrolisis enzimatik protein kulit ayam dengan menggunakan variasi konsentrasi enzim papain. Proses hidrolisis dilakukan dengan variasi konsentrasi enzim 1, 3,dan 5% (b/b protein), pada pH 7, suhu 50oC dan waktu hidrolisis 24 jam. Parameter yang diukur meliputi derajat hidrolisis, persen peredaman radikal DPPH (diphenylpicryl hidrazyl), dan komposisi asam amino hidrolisat protein yang paling aktif antioksidan. Hasil penelitian menunjukkan penggunaan konsentrasi enzim yang berbeda menghasikan hidrolisat protein dengan derajat hidrolisis dan persen peredaman DPPH yang berbeda. Penggunaan konsentrasi enzim papain 1, 3, dan 5% menghasilkan hidrolisat protein dengan derajat hidrolisis berturut-turut sebesar 9,3±1,8, 18,09±5,6, dan 23,15±6.33%, dan persentase hambatan radikal DPPH pada konsentrasi uji 100 ppm berturut-turut sebesar 16,75±0,07, 58,35±0,00, dan 52,99±0,07%. Hidrolisat protein yang diperoleh menggunakan enzim papain 3% (b/b protein) menunjukkan persen peredaman tertinggi dengan nilai IC50 92,98 ppm, memiliki komposisi asam amino tertiggi asam glutamat dan terendah valine. Kata kunci: antioksidan, enzim papain, hidrolisat protein, kulit ayam This study aimed to determine the antioxidant activity of protein hydrolyzate produced from the enzymatic hydrolysis of chicken skin protein by using variations of the enzyme papain concentration. The hydrolysis process was carried out with variations in the concentration of the enzymes 1, 3, and 5% (w/w protein base), at pH 7, temperature 50oC, and hydrolysis time 24 hours. The parameters measured including degree of hydrolysis, percentage of inhibition of DPPH radical (diphenylpicryl hydrazyl) and amino acid composition of the protein hydrolyzate with the highest antioxidant activity. The results showed that the use of different enzyme concentrations resulted in protein hydrolyzate with different degrees of hydrolysis and percentage of inhibition of DPPH. The use of papain enzymes 1, 3, and 5% produced protein hydrolyzate with the degree of hydrolysis of 9.3 ± 1.8, 18.09 ± 5.6, and 23.15 ± 6.33%, and the percentage of inhibition of DPPH radical at a concentration of 100 ppm 16.75 ± 0.07, 58.35 ± 0.00, and 52.99 ± 0.07% respectively. Protein hydrolyzate obtained using the enzyme papain 3% (w/w protein) exhibited the highest percentage of inhibition of DPPH with an IC50 value of 92.98 ppm, having the highest amino acid composition of glutamic acid and the lowest valine. Keywords: antioxidant, enzyme papain, protein hydrolyzate, chicken skin

EKSTRAKSI ZAT WARNA ALAM DARI BONGGOL TANAMAN PISANG (Musa paradiasciaca L.) DENGAN METODE MASERASI, REFLUKS, DAN SOKLETASI Anak Agung Bawa Putra; Ni Wayan Bogoriani; Ni Putu Diantariani; Ni Luh Utari Sumadewi
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 1 Januari 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

We have conducted research on natural dyes extraction of banana (Musa paradiasiaca L.) weevil. The rendement concentration, its color, and their functional groups were determined using phytochemical test and ultraviolet-visible spectrophotometer. Extraction of natural dyes in the study was conducted by three methods namely maceration, reflux, and soxletation by using four kinds of extracting solvent including water, ethanol, acetone, and n-hexane. Color extract in water was dark brown, in ethanol and acetone was light brown, and in n-hexane was yellow. Each extract obtained by the three methods of each solvent was concentrated, their rendement were determined. The yields obtained by the maceration method using water was 8.12%, ethanol 2.40%, acetone 0.52%, and n-hexane 1.16%. The yields of the reflux method were 8.68%, 1.84%, 1.44%, and 1.04% respectively. The yields of the soxletation method were 4.80%, 1.12%, 0.44%, 0.56% respectively. The phytochemical test showed that the banana weevil dyes contained tannins and flavonoids. Absorption of the wavelength of energy ultra violet – visible detected at a wavelength between 200 nm up to 400 nm.

ISOLASI DAN IDENTIFIKASI GLIKOSIDA STEROID DARI DAUN ANDONG (Cordyline terminalis Kunth) N. W. Bogoriani
Jurnal Kimia (Journal of Chemistry) Vol. 2, No. 1 Januari 2008
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.181 KB)

Isolation and identification of two steroid glicosides have been conducted from Andong leaves (Cordylineterminalis Kunth). The isolate (4,0 mg white amorphous solid) was obtained after a series of chromatographicseparations. Identification of the isolate using mass spectrometry with positive electrospray showed MW 868 ascalculated from the ion peaks of m/z 891[M + Na]+, and 869[M + H]+. The ion peaks of isolate at m/z 727[(M +Na) - 164]+, 723[(M + H) - 146]+, 705[(M + H) - 164]+, and 413 [(M + H) - 456]+ of its fragments indicated thepresence of three sugars (two terminal sugars and one central sugar) from methylpentose moeity (each of MW 164)linked to an agyicone. Proton magnetic resonance spectrum of the isolate in pyridine-d5 showed characteristicproton signals for three steroid methyls (two angular methyls and one secondary methyl) at d 1.37 (s), 0.85 (s) and1.06 (d, J = 6 Hz); and one methyl group for C25 at d 0,66 ppm (d, J=6 Hz); an ethylene group at d 5.51 ppm (br d, J= 5.7 Hz); signals of the protons linked to C26 at d 4.13 and 3,49 ppm (each br d, J = 9,3 Hz and 9 Hz), and threeanomeric protons at d 6.43 ppm (br s); 5.56 ppm (br s) and 4.57 ppm (d, J = 7.0 Hz). From the above data it can beassumed that the isolate is a spirostan steroidal glicoside.

Co-Authors A. A. Bawa Putra A. Intan Saridewi Abd. Rasyid Syamsuri Anak Agung Istri Agung Mayun Laksmiwati Dwi Cahya Putra I M E. P. Siregar I M, Sukadana I Nengah Wirajana I Wayan Suirta I. A. R. Astiti Asih Jeane, Magda Mahayani, Putu Ayu Erna Miftakul Sururi N. M. T. Juliasari Ni Komang Ariati Ni Luh Rustini Ni Luh Utari Sumadewi Ni Made Puspawati Ni Made Suaniti Ni Pt. Pande Mirah Surya Dewi Ni Putu Diantariani Nur Hafsia P. P. Dewi P. Suarya Sri Rahayu Santi Sri Wahjuni Sri Wardani

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