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All Journal Biospecies JOURNAL OF COASTAL DEVELOPMENT Berkala Perikanan Terubuk Journal of Aquatropica Asia Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Esti Harpeni
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Other

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EFFECTIVITY OF LIGHT INTENSITY ON COLOR GRADATION AND CAROTENOIDS CONTENT OF Lobophyllia hemprichii Ahmad Mustawa; Esti Harpeni; Moh. Muhaemin
AQUASAINS Vol 5, No 2 (2017)
Publisher : Jurusan Perikanan dan Kelautan

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Abstract

L. hemprichii is one of the marine ecosystems with high biodiversity and the most productive. The more healthy corals, the higher productivity in the sea. The existence of light is needed for L. hemprichii for its growth. Moreover L. hemprichii has color that produced by symbiotic algae pigments such as carotenoid pigments. Carotenoid pigment has active role to absorb light in process of photosynthetic of algae symbionts. The aims of this study were to gain different effects of  light intensity to color gradation of red and carotenoids content of L. hemprichii. The study was conducted by placing L. hemprichii into aquriums with ICR treatment (Low Light Intensity = 1514 Lux), ICS treatment (Medium Light Intensity = 3028 Lux), treatment ICR (High Light Intensity = 4547 Lux). The results showed that the light intensity affect the color gradation of  coral L. hemprichii and light intensity affect the concentration of total carotenoid pigment L. hempirchii. The best light intensity for L. hempirchii color and carotenoid pigment production was high light intensity (4547 Lux).
EFFECT OF LIGHT INTENSITY ON THE CELL DENSITY, DIAMETER AND CELL VOLUME ZOOXANTHELLAE FROM ISOLATE SOFTCORAL Zoanthus sp Suliswati Suliswati; Esti Harpeni; Moh. Muhaemin
AQUASAINS Vol 5, No 1 (2016)
Publisher : Jurusan Perikanan dan Kelautan

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Abstract

Coral reefs are one of the aquatic ecosystem trophic productive for aquatic organisms. Coral reefs can not be separated from the various threat of causing damage. Physiological damage that is visible coral bleaching. Bleaching of coral reefs due to causes it may be the biggest threat to coral ecosystems due to widespread in various regions. Coral bleaching causing the loss of endosymbiont coral or reduction of photosynthetic pigments zooxanthellae of corals. Zooxanthellae need light for photosynthesis. The process will produce energy which serves for the biosynthesis cell, growth and cell division. Therefore light plays an important role in the process of photosynthesis. This research aims were to know the cell density, diameter and volume zooxanthellae cells on different light intensity. The research was conducted on July-August 2016, in the Aquaculture Laboratory, Program Study of Aquaculture Faculty of Agriculture University of Lampung. Observed parameters were the density, diameter and volume zooxanthellae cells. Research used the 4 treatments and 5 replications namely 3800 lux (IC1), 6250 lux (IC2), 7980 lux (IC3), and 11800 lux (IC4). Measurement of the density and diameter of zooxanthellae cells used a hemocytometer neubauer improved and objective micrometer (0.01 mm). Data were analyzed by analysis of variance (ANOVA) and followed by LSD test. The results showed that the intensity of light significantly affected the density, diameter and volume zooxanthellae cells. Intensity light given by zooxanthellae responded with increased density and a decrease the diameter and volume zooxanthellae cells in the early stages of culture (0-18 hours).
Penapisan Bakteri Pendegradasi Total Ammonia Nitrogen dari Sedimen Tambak Tradisional Udang Windu (Penaeus monodon) Eva Susanti; Esti Harpeni; Agus Setyawan; Berta Putri
AQUASAINS Vol 2, No 2 (2014)
Publisher : Jurusan Perikanan dan Kelautan

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Abstract

Bioremediasi merupakan salah satu upaya untuk mengurangi limbah tambak dengan memanfaatkan kemampuan mikroorganisme. Tujuan penelitian ini adalah untuk mendapatkan kandidat isolat bakteri bioremediasi yang mampu mendegradasi total ammonia nitrogen (TAN) dari sedimen tambak tradisional.  Sedimen didapat dari tiga tambak udang windu (Penaeus monodon) di Desa Mulyosari, Kecamatan Pasir Sakti, Kabupaten Lampung Timur, Provinsi Lampung dengan jarak tambak dari pantai yaitu antara 600 - 1800 m). Metode phenate digunakan untuk menguji pendegradasi TAN. Hasil penelitian menunjukkan terdapat tiga isolat bakteri terbaik yang mampu menurunkan kandungan TAN adalah TI6, TI1, dan TII5 sebanyak 0,10; 0,06 mg/l dan 0,06 mg/l secara berurutan. Ketiga isolat tersebut diidentifikasi sebagai genus Campylobacter, Listeria dan Nitrosococcus.
PATHOGENICITY AND IN VIVO STUDY OF LOCAL ISOLATE Bacillus sp. D2.2 AT THE VANNAMEI CULTURE (Litopenaeus vannamei) Sera Hardiyani; Esti Harpeni; Agus Setyawan; Supono Supono
AQUASAINS Vol 5, No 1 (2016)
Publisher : Jurusan Perikanan dan Kelautan

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Abstract

Penggunaan bakteri biokontrol dapat dijadikan solusi bagi permasalahan pemberantasan penyakit untuk menekan pertumbuhan bakteri pathogen pada budidaya udang vaname (Litopenaeus vannamei). Bacillus sp. D2.2 merupakan isolat bakteri lokal yang terbukti mampu menghambat pertumbuhan Vibrio harveyi secara in vitro. Potensi lain isolat bakteri ini perlu diketahui lebih lanjut melalui penelitian ini. Tujuan penelitian ini adalah untuk mengetahui tingkat patogenisitas bakteri biokontrol Bacillus sp. D2.2 terhadap udang vaname dan kemampuannya dalam menghambat pertumbuhan bakteri patogen Vibrio alginolyticus secara in vivo. Uji patogenisitas Bacillus sp. D2.2 dilakukan dengan metode LD50 pada tingkat kepadatan 103, 104, 105 dan 106 CFU/ml.Hasil LD50 menunjukkan Bacillus sp. D2.2 tidak bersifat patogen karena tidak ada konsentrasi bakteri yang mematikan hingga 50% larva udang vaname. Uji antagonisme Bacillus sp. D2.2 terhadap V.alginolyticus secara in vivo dilakukan pada 2 perlakuan, yaitu pemeliharaan udang vaname tanpa penambahan Bacillus sp. D2.2 dan pemeliharaan udang vaname dengan penambahan Bacillus sp. D2.2. Kedua perlakuan diuji tantang dengan V.alginolyticus 105 cfu/ml dan diulang 3 kali. Hasil penelitian menunjukkan Bacillus sp. D2.2 mampu menurunkan pertumbuhan V.alginolyticus dari 105 CFU/ml sampai 103 CFU/ml. Hal tersebut menjelaskan bahwa Bacillus sp. D2.2 berpotensi sebagai bakteri biokontrol.
PENGARUH SUBTITUSI TEPUNG IKAN DENGAN TEPUNG CACING TANAH (Lumbricus sp.) TERHADAP PERTUMBUHAN BENUR UDANG WINDU (Penaeus monodon) Muhammad Ridho Taris; Limin Santoso; Esti Harpeni
e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Vol 6, No 2 (2018)
Publisher : University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.409 KB) | DOI: 10.23960/jrtbp.v6i2.p699-704

Abstract

Availability of good-quality shrimp feed is an important factor of shrimp increased production. High protein feed can be generated by adding raw materials that contains high protein. One of the raw materials that contains high protein is earthworm meal (Lumbricus sp.). This research goal was to study the influence of the substitution of fish meal by earthworm feed with different proportions toward growth and survival rate of tiger shrimp larvae (Penaeus monodon). The experimental design used Completely Randomized Design (RAL) consists of five treatments with three replications: Feed test A (25%  earthworm meal + 75% fish meal), feed test B (50% earthworm meal + 50% fish meal), feed test C (75% earthworms meal + 25% fish meal), feed test D (100% earthworms meal) and feed test E (commercial feed). Results showed that the tiger shrimp which given feed test E is the best result for tiger shrimp growth with 0.480 ± 0.076 grams/individual, while feed test B was the best result for tiger shrimp survival rate with 82 ± 3.46%., and on the feed test D was the best result for tiger shrimp feed conversion rate with 4.0 ± 0.17. Based on results of statistical tests showed that the substitution of fish meal with earthworm meal did not give significant effect on growth and survival rate of tiger shrimp.
IMUNOGENISITAS KOMBINASI VAKSIN INAKTIF WHOLE CELL Aeromonas salmonicida DAN VITAMIN C PADA IKAN MAS (Cyprinus carpio) Nurma Jana Hazzulli; Agus Setyawan; Esti Harpeni
e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Vol 3, No 2 (2015)
Publisher : University of Lampung

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Abstract

Penambahan vitamin C dalam vaksin diketahui dapat meningkatkan imunogenisitas dan efektivitas vaksin.  Penelitian ini bertujuan untuk meningkatkan imunogenisitas vaksin inaktif whole cell A. salmonicida dengan penambahan vitamin C pada ikan mas (Cyprinus carpio).  A. salmonicida diinaktifasi dengan formalin 1,5 % (v/v). Vaksin I disuntikkan secara intra peritoneal (i.p) (107 sel / ikan) kepada ikan mas (ukuran ± 30gr).  Vaksin II (Booster) dilakukan 7 hari setelah vaksinasi I dengan metode dan dosis yang sama.  Pengambilan darah (Bleeding) dilakukan sebelum vaksinasi, 7 hari setelah vaksinasi I, dan 7 hari setelah vaksinasi II.  Analisis darah meliputi titer antibodi, hematokrit, dan total leukosit diamati setiap pengambilan sampel darah.  Hasil penelitian menunjukkan penambahan 1000 mg vitamin C dalam vaksin memiliki nilai titer antibodi, hematokrit, dan total leukosit paling tinggi dari perlakuan lainnya yaitu 28, 28%, 88.000 sel/mm3, secara berturut-turut pada pengamatan terakhir (7 hari setelah booster).
Pengaruh Waktu Pemberian Probiotik Yang Berbeda Terhadap Respon Imun NonSpesifik Ikan Mas (Cyprinus Carpio L.) Yang Diuji Tantang Dengan Bakteri Aeromonas Salmonicida Septiarini -; Esti Harpeni; Wardiyanto -
e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Vol 1, No 1 (2012)
Publisher : University of Lampung

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Abstract

Common carp (Cyprinus carpio) is one of the consumption fish which has high economic value. However, the efforts to increase carp production obstracted by diseases such as furunculosis by A. salmonicida. So that we have to consider the disease prevention method which safer such as probiotic. The aims of this research were to know effect of the time administrations of probiotic on non-specific immune responses and to know the best time administration of probiotic on non-specific immune responses of common carp injected by A. salmonicida. The research was conducted from August to October 2011. The research used three treatments (without administration of probiotic, administration of probiotic once every day, and administration of probiotic once every five days) with four replications. Data of total leukocyte and percentage of differential leukocyte were analyzed by using ANOVA, while RPS and water qualities were analyzed descriptively. The results showed the time administrations of probiotic showed a real impact on improving non-specific immune responses characterized by increased total leukocyte in carp, administration of probiotic once every five days resulted better non-specific immune responses, which had been seen from the highest total leukocyte and the highest RPS after being injected by A. salmonicida.
Eksplorasi Bakteri yang Berasosiasi dengan Karang Lunak sebagai Alternatif Sumber Senyawa Bioaktif: Uji Hayati Antibakteri Esti Harpeni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 3 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.3.283

Abstract

Soft coral has a significant function in the ecology of coral reef which produces secondary metabolites. One of very serious problems with coral reef ecosystem related to secondary metabolite improvement was coral supply because of need of many soft corals producing a little amount of secondary metabolites. The purposes of this research were to isolate and to select soft coral bacteria that have ability to produce antibacterial having been used to inhibit Escherichia coli and Staphylococcus aureus. The research was conducted from March 31th to November 2nd, 2005 by using description method. Sampling was carried out at Pulau Tegal Waters on two different coral genera, namely Briareum and Sinularia at three different sites. Laboratory studies were performed at Environment and Healthy Monitoring Fish Laboratory at Lampung Marine Agricultural. The results showed that five of 125 isolates obtained had potential of producing antibacterial that have been used to inhibit E.coli and S. aureus. The coral ability to inhibit E.coli and S. aureus was indicated by inhibition zone around soft coral bacteria colonies. Morphology and biochemistry characterization of potentially inhibiting isolates against E. coli and S. aureus resulted in strains Staphylococcus sp. (isolate code B1I10), Plesiomonas sp. (isolate code B1I11), Actinobacillus sp. (isolate code B2I6), Actinomyces sp. (isolate code B1II4), and    Aerococcus sp. (isolate code S1III2). 
Screening of Potential Probiotic Vibrio sp. Against Vibriosis in the Litopenaeus vannamei Munti Sarida; Esti Harpeni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 2 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.2.197

Abstract

Vibriosis is a bacterial disease generated by Vibrio sp., particularly V. harveyi that becomes the biggest cause of up to 100% shrimp mortality. Using antibiotics as the curative step is not applicable anymore, because of the residual effects to the shrimp and environment and the resistant effects to the humans as well. The purpose of this study was to screen potentially probiotic from strain Vibrio sp. that could inhibit V. harveyi as an effort of handling vibriosis diseases. Water sample from the shrimp ponds were isolated and purified in SWC-agar and TCBS-agar and then were incubated overnight at the room temperature. Bacterial concentrations were calculated using MC Pharland method and spectrophotometer. In vivo test was conducted for the best candidate of potential probiotic isolate, i.e. CP1. Challenged test was leaded within 4 different bacterial concentrations as the treatments and 2 replications using 10 shrimps as the tested organisms per aquarium. The treatments were 107 CFU/ml CP1 VS 107 V. harveyi MR5339 RifR (1), 106 CFU/ml CP1 VS 107 V. harveyi MR5339 RifR (2), 105 CFU/ml CP1 VS 107 V. harveyi MR5339 RifR (3) and PBS (4) as the control.  Our study showed that the Survival Rate of the tested organisms was 90% in the treatment 1. This result indicated the capability of growth inhibition of the candidate probiotic bacterium against V. harveyi. Presumably, Vibrio sp. could be used in the vanamei culture as the probiotic. Finally, the candidate probiotic bacterium was identified as Vibrio furnissii.
Penanganan penyakit white feces pada udang vaname Litopenaeus vannamei menggunakan aplikasi pakan yang dicampur ekstrak lengkuas merah Alpinia purpurata k. schum Juliana Marbun; Esti Harpeni; Wardiyanto Wardiyanto
Depik Vol 8, No 2 (2019): August 2019
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.021 KB) | DOI: 10.13170/depik.8.2.13570

Abstract

Abstract. The main problem in vaname shrimp Litopenaeus vannamei culture is a disease. One of the diseases that can infect vaname shrimp is White Feces Disease (WFD) caused by bacteria Vibrio sp. The Treatment can be done by using Red galangal Rhizome extract. Since MBC testing result galangal extract can be a treat of Vibrio sp bacteria. This research used the completely randomized design (RAL) with five treatments and three replications which treatment A (negative control),          B (positive control), C (7.5 grams), D (10 grams) and (12.5 grams) which are mixed with pellet for treating White Feces Disease in vaname shrimp. The result shows that red galangal rhizome extracted give effect to SR (Survival Rate), RPS (Relative Percent Survival), TVC (Total Vibrio Count), but it didn’t give effect to clinical indication and   histopathology.Keywords: vaname shrimp, white feces disease, extract red galangal Rhizome Abstrak. Permasalahan utama dalam budidaya udang vaname (Litopenaeus vannamei) adalah penyakit, salah satu penyakit yang dapat menyerang udang vaname yaitu White Feces Disease (WFD) yang disebabkan bakteri Vibrio sp. Upaya pengobatan yang dapat dilakukan untuk menangani penyakit tersebut adalah dengan pengobatan menggunakan ekstrak rimpang lengkuas merah dikarenakan ekstrak lengkuas telah diuji secara MBC dapat mematikan bakteri Vibrio sp. Penelitian ini bertujuan untuk menguji ekstrak rimpang lengkuas merah dengan dosis berbeda yang dicampur dengan pakan buatan untuk mengobati penyakit white feces disease pada udang vaname (Litopenaeus vannamei). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan lima perlakuan, tiga ulangan dimana perlakuan A (kontrol negatif), perlakuan B (kontrol positif), perlakuan C (7,5 g), perlakuan D (10 g) dan perlakuan E (12,5 g). Hasil penelitian menunjukan bahwa ekstrak rimpang lengkuas merah berpengaruh terhadap SR, RPS (Relative Percent Survival) dan TVC (Total Vibrio Count), namun tidak berpengaruh terhadap gejala klinis dan histopatalogi. Kata Kunci: udang vaname, white feces disease, ekstrak rimpang lengkuas merah
Co-Authors Agus Setyawan Ahmad Mustawa Arlin Wijayanti Arthur Lemuel David Asep Saefulloh Assovaria . Berta Putri Eko EFFENDI Eva Susanti Hilma Putri Fidyandini Ignatius Sandra Setyabudi Juliana Marbun Limin Santoso Moh. Muhaemin Muhammad Ridho Taris Munti Sarida Nandya Dwinitasari Novi Santika Nurma Jana Hazzulli Rara Diantari Rico Wahyu Prabowo Rissa Amelia Septiarini - Sera Hardiyani Sri Waluyo Suliswati Suliswati Supono Supono Syohibahttul Islamiyah BAHAR Triga Royana Sagala Uun Febriyani Wardiyanto - Wardiyanto Wardiyanto Yudha Trinoegraha Adiputra