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All Journal Journal of Aquatropica Asia
Tiara Puspa Anjani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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OPTIMIZATION OF ANNEALING TEMPERATURE FOR COI GENE AMPLIFICATION ON EXTRIMOPHIL FISH USING REAL-TIME PCR ardiansyah kurniawan; Tiara Puspa Anjani; Eva Lestari; Alya M Safitri; Andri kurniawan; ahmad fahrul syarif; Rina Apriyani; Merin S; Muhamad Ichsan; Siti P N I K Almagribi
Journal of Aquatropica Asia Vol 7 No 2 (2022): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/joaa.v7i2.3401

Abstract

Extremophilic fish require genetic characterization to determine the identity of their species, their kinship, and the potential for genetic variation due to extreme environmental adaptation. DNA barcoding using Fish-F2 and Fish-R2 primers at an annealing temperature of 52oC for 15 seconds showed qualitative failure results in most fish species. For this reason, optimizing the annealing temperature in the PCR process is necessary to obtain DNA bands that correlate with successful identification. Fish samples were obtained from Ruai Silip, Bangka Island, Membalong, Belitung Island, and Way Kanan, Lampung, Sumatra Island. The annealing temperature optimization was set at 50, 50.4, 51.1, 52.3, 53.7, 54.8, 55.5, and 56°C. Five species of extremophile fish were tested, namely Brevibora sp, Barbodes binotatus, Rasbora bankanensis, Anabas testudineus, and Aplocheilus panchax. Extremophile fish showed differences in the appearance of DNA bands in PCR with different annealing temperatures. Brevibora sp showed a visualization of DNA bands at 54.8°C, Barbodes binotatus at 53.7, 54.8, 55.5, and 56°C, Anabas testudineus at 50 and 50.4°C, and Aplocheilus panchax produced clearly visible band on annealing temperatures.
POLICY FOR THE DEVELOPMENT OF CULTIVATED FISHERY COMMERCE IN THE PROVINCE OF THE BANGKA BELITUNG ISLANDS Endang Bidayani; Tiara Puspa Anjani; Muhammad Fachri
Journal of Aquatropica Asia Vol 8 No 2 (2023): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/joaa.v8i1.4533

Abstract

The trading system for aquaculture products is the process of distributing goods in the form of fish or derivative products from producers to final consumers effectively and efficiently. The objectives of this research are: 1) Analyzing aquaculture supply chain management in the Bangka Belitung Islands Province; and 2) Develop recommendations for the direction of development of the aquaculture trade system. This research method is a survey. The data sources in this research are primary and secondary data. The primary data collection method is qualitative, namely the researcher makes questionnaires, observations and interviews. Secondary data comes from production data from the Fisheries Service/BPS/pokdakan/regency/city companies in the Bangka Belitung Islands Province. Respondents in this study were informants at the District/City Fisheries Service, Pokdakan, and the shrimp farming industry (micro/small/medium/large scale). Data analysis is descriptive. The research results show that the grouper aquaculture supply chain is more efficient than the shrimp aquaculture supply chain, because it involves more companies from outside the Bangka Belitung Islands Province. The direction of development of the aquaculture trade system which refers to supply chain management has not yet implemented Minister of Maritime Affairs and Fisheries Regulation No. 5/Permen-KP/2014 concerning the National Fish Logistics System (SLIN). Understanding of the cold chain is still low. Then the problem at the collection and storage stage is the availability of facilities (cold storage) as well as the availability of basic infrastructure such as electricity, clean water and others, and the availability of facilities (unplug reefer).
Co-Authors Ahmad Fahrul Syarif Alya M Safitri ANDRI KURNIAWAN Ardiansyah Kurniawan Endang Bidayani Eva Lestari Merin S Muhamad Ichsan Muhammad Fachri Rina Apriyani Siti P N I K Almagribi