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All Journal Journal of Aquatropica Asia JITPI : Jurnal Ilmu Dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Science and Technology)
Muhamad Ichsan
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Skrening Resistensi Antibiotik Pada Bakteri Asam Laktat yang Diisolasi dari Usus Ayam Pedaging Muhamad Ali; Nurbaiti Nurbaiti; Anwar Rosyidi; Muhamad Ichsan
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 4 No 1 (2018): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.02 KB) | DOI: 10.29303/jitpi.v3i1.39

Abstract

Antibiotic use in poultry production is commonly performed as the feed additive for promoting the health and growth of the chicken. However, excessive use of antibiotics may lead to the emergence of antibiotic resistance in Escherichia coli, lactic acid bacteria, and pathogenic bacteria. In this research, screening of antibiotic resistance was assayed on lactic acid bacteria obtained from broiler intestine. Among the 14 lactic acid isolates, 2 (14%) isolates were found to be resistant to chloramphenicol. Resistance was also observed to ampicillin and kanamycin (7%). The result of this research indicated that the overuse and misuse of antibiotics in broiler farm tend to generate antibiotic resistance in broiler’s intestine lactic acid bacteria. Therefore, some advice for safety measure of antibiotic application in poultry farmer is strongly necessary.
OPTIMIZATION OF ANNEALING TEMPERATURE FOR COI GENE AMPLIFICATION ON EXTRIMOPHIL FISH USING REAL-TIME PCR ardiansyah kurniawan; Tiara Puspa Anjani; Eva Lestari; Alya M Safitri; Andri kurniawan; ahmad fahrul syarif; Rina Apriyani; Merin S; Muhamad Ichsan; Siti P N I K Almagribi
Journal of Aquatropica Asia Vol 7 No 2 (2022): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/joaa.v7i2.3401

Abstract

Extremophilic fish require genetic characterization to determine the identity of their species, their kinship, and the potential for genetic variation due to extreme environmental adaptation. DNA barcoding using Fish-F2 and Fish-R2 primers at an annealing temperature of 52oC for 15 seconds showed qualitative failure results in most fish species. For this reason, optimizing the annealing temperature in the PCR process is necessary to obtain DNA bands that correlate with successful identification. Fish samples were obtained from Ruai Silip, Bangka Island, Membalong, Belitung Island, and Way Kanan, Lampung, Sumatra Island. The annealing temperature optimization was set at 50, 50.4, 51.1, 52.3, 53.7, 54.8, 55.5, and 56°C. Five species of extremophile fish were tested, namely Brevibora sp, Barbodes binotatus, Rasbora bankanensis, Anabas testudineus, and Aplocheilus panchax. Extremophile fish showed differences in the appearance of DNA bands in PCR with different annealing temperatures. Brevibora sp showed a visualization of DNA bands at 54.8°C, Barbodes binotatus at 53.7, 54.8, 55.5, and 56°C, Anabas testudineus at 50 and 50.4°C, and Aplocheilus panchax produced clearly visible band on annealing temperatures.
Co-Authors Ahmad Fahrul Syarif Alya M Safitri ANDRI KURNIAWAN Anwar Rosyidi Ardiansyah Kurniawan Eva Lestari Merin S Muhamad Ali Nurbaiti Nurbaiti Rina Apriyani Siti P N I K Almagribi Tiara Puspa Anjani