Article Per Year (5 Year)
p-Index From 2019 - 2024
0.23
P-Index
This Author published in this journals
All Journal Journal of Aquatropica Asia
Alya M Safitri
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
OPTIMIZATION OF ANNEALING TEMPERATURE FOR COI GENE AMPLIFICATION ON EXTRIMOPHIL FISH USING REAL-TIME PCR ardiansyah kurniawan; Tiara Puspa Anjani; Eva Lestari; Alya M Safitri; Andri kurniawan; ahmad fahrul syarif; Rina Apriyani; Merin S; Muhamad Ichsan; Siti P N I K Almagribi
Journal of Aquatropica Asia Vol 7 No 2 (2022): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/joaa.v7i2.3401

Abstract

Extremophilic fish require genetic characterization to determine the identity of their species, their kinship, and the potential for genetic variation due to extreme environmental adaptation. DNA barcoding using Fish-F2 and Fish-R2 primers at an annealing temperature of 52oC for 15 seconds showed qualitative failure results in most fish species. For this reason, optimizing the annealing temperature in the PCR process is necessary to obtain DNA bands that correlate with successful identification. Fish samples were obtained from Ruai Silip, Bangka Island, Membalong, Belitung Island, and Way Kanan, Lampung, Sumatra Island. The annealing temperature optimization was set at 50, 50.4, 51.1, 52.3, 53.7, 54.8, 55.5, and 56°C. Five species of extremophile fish were tested, namely Brevibora sp, Barbodes binotatus, Rasbora bankanensis, Anabas testudineus, and Aplocheilus panchax. Extremophile fish showed differences in the appearance of DNA bands in PCR with different annealing temperatures. Brevibora sp showed a visualization of DNA bands at 54.8°C, Barbodes binotatus at 53.7, 54.8, 55.5, and 56°C, Anabas testudineus at 50 and 50.4°C, and Aplocheilus panchax produced clearly visible band on annealing temperatures.
Co-Authors Ahmad Fahrul Syarif ANDRI KURNIAWAN Ardiansyah Kurniawan Eva Lestari Merin S Muhamad Ichsan Rina Apriyani Siti P N I K Almagribi Tiara Puspa Anjani