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Anti-migration Effect of Aaptos suberitoides Fraction in HCT-116 Colorectal Cancer Cell Line Dany Muhammad Daffa; Muhammad Hasan Bashari; Eko Fuji Ariyanto; Tenny Putri; Nurul Qomarilla
Althea Medical Journal Vol 8, No 1 (2021)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v8n1.2231

Abstract

Background: Colorectal cancer is the second leading cause of mortality and the most prevalent cancer worldwide. Most patients, who come with late-stage, have ineffective treatments and some side effects in chemotherapy. Aaptos suberitoides has potential anti-cancer effects due to its bioactive compounds such as aptamine. This study aimed to evaluate the migration inhibition effect of Aaptos suberitoides fraction in HCT-116 cell line.Methods: This study was an experimental study. Aaptos suberitoides specimen was taken in Tinjil Island and fractionated with ethyl acetate. HCT-116 cell line was added with Aaptos suberitoides fraction and cellular migration activity was observed in 48 hours of which the scratch assay was performed. The gap closure area was determined with ImageJ software.Results: The data showed that a low concentration of Aaptos suberitoides fraction inhibited migration activity in HCT-116 cell line as follow; 1 and 5 mg/L Aaptos suberitoides fraction inhibit 3-4 % cancer cell migration in 24 hours, and 10-11% inhibition in 48 hours, respectively. However, 10 mg/L fraction concentration only inhibited 7-14% of the migration effect.Conclusion: Aaptos suberitoides fraction suggests insignificant migration inhibition in colorectal cancer cells and only inhibits less than 15 % HCT-116 cell line.
Kebijakan Medik pada Pasien Gagal Ginjal Kronik dengan Hemodialisis di RS Hasan Sadikin Bandung Dewi Marhaeni Diah Herawati; Eko Fuji Ariyanto
Jurnal Kebijakan Kesehatan Indonesia Vol 3, No 2 (2014)
Publisher : Center for Health Policy and Management

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.669 KB) | DOI: 10.22146/jkki.36360

Abstract

Background: Prevalence of Chronic Kidney Disease in dialysis’s patients in Indonesia has increased. Some of them occurred with malnutrition inflammation complex syndrome and lead to death. This study aims to determine the intake of protein and energy, and determine factors that cause the low intake of nutrition. Methods: Design of the study was mixed methods using embedded concurrent strategy. Research paradigm was constructivism whereas qualitative research conducted in-depth interviews and observations. Quantitative research has been done with a descriptive approach, observational, using secondary data and perform 24 Hour Recall and Food Frequency Questionnaire (FFQ). The study was conducted at Hemodialysis Unit, Hasan Sadikin Hospital from June to September 2013, with a total sampling. Qualitative and quantitative data analysis has been done, followed by analysis of policy and analysis for policy for establishing a medical policy for chronic kidney disease patients receiving medical hemodialysis. Result: The average protein intake of the patients was 1.32 g/ kg/day. Interval of protein intake of 0.5 g/kg /day (lowest) until 2.8 g/kg/day (highest). 24% of patients had protein intake under 1 g /kg BW/day and 22.8% was above 1.5 g/kg BW/day. Average energy intake was 2001 kcal patient/day (930 kcal/ day - 3196.9 kcal/day). The qualitative analysis resulted in seven themes which causes nutrient low intake. The themes were underlying diseases (such as diabetes mellitus and hypertension), length of dialysis, frequency and number of dialysis, effects of dialysis, body’s response, cost factor, counseling, and education. Most of the respondents felt suffer from anemia and complained of nausea and vomiting. Body responses varied widely among them. Conclusion: Protein intake of dialysis patients as recommended by K/DOQI, but not in accordance with energy intake. Protein and energy intake of Jakeman's holder patients were lower than the recommendation of K/DOQI. The cause of lower intake of nutrients due to the underlying disease, length of dialysis, frequency and number of dialysis, effects of dialysis, body responses, cost factors and lack of counseling and education. Counseling and education of the patient's hospital are needed. The government should encourage medical policy in the management of patients with chronic kidney failure are comprehensive, in primary care, secondary and tertiary. The government must provide competent personnel, facilities and supporting infrastructure, service standards and standard operating procedures are required for each level of service. Latar Belakang: Prevalensi pasien Penyakit Ginjal Kronik (PGK) yang menjalani hemodialisis di Indonesia mengalami peningkatan. Beberapa diantaranya terjadi malnutrition inflammation complex syndrome dan berujung pada kematian. Penelitian bertujuan untuk mengetahui asupan protein dan energi, serta mengetahui faktor-faktor yang menyebabkan rendahnya asupan nutrisi. Metode: Desain penelitian adalah mixed method dengan menggunakan strategi conccurent embedded. Paradigma penelitian adalah constructivisme. Penelitian kuantitatif dengan pendekatan deskriptif, observational menggunakan data skunder dan melakukan 24 Hour Recall dan Food Frequency Questionaire (FFQ). Penelitian kualitatif dilakukan dengan wawancara mendalam dan observasi. Penelitian dilakukan di Unit Hemodialisis Rumah Sakit Hasan Sadikin Bandung bulan Juni-September 2013 dengan total sampling. Dilakukan analisis data kualitatif dan kuantitatif yang diikuti analysis of policy dan analysis for policy untuk merumuskan kebijakan medik pada pasien PGK yang menjalani hemodialisis. Hasil: Rata-rata asupan protein pasien adalah 1,32 gr/kg BB/ Hari. Asupan protein terendah 0,5 gr/kg BB/hari, tertinggi 2,8 gr/kg BB/hari. Asupan protein pada 24% pasien dibawah 1 gr/ kg BB/hari; 22,8% diatas 1,5 gr/kg BB/hari. Rata-rata asupan energi pasien 2001 kkal/hari, asupan terendah 930 kkal/hari, tertinggi 3196,9 kkal/hari. Analisis kualitatif menghasilkan 7 tema yang menjadi penyebab rendahnya asupan nutrisi yaitu penyakit dasar (diabetes mellitus, hipertensi), lama dialisis, frekuensi dan jumlah dialisis, efek dialisis, respon tubuh, faktor biaya dan konseling serta edukasi. Efek dialisis yang paling dikeluhkan adalah anemia, mual dan muntah. Respon tubuh diantara pasien sangat bervariasi. Konseling dan edukasi dari pihak rumah sakit sangat dibutuhkan pasien. Kesimpulan: Asupan protein pasien sesuai rekomendasi K/ DOQI, namun belum sesuai untuk asupan energi. Asupan protein dan energi pasien Jamkesmas lebih rendah dari rekomendasi K/DOQI. Penyebab rendahnya asupan nutrisi disebabkan karena penyakit dasar yang menjadi penyebab PGK, lama dialisis, frekuensi dan jumlah dialisis, efek dialisis, respon tubuh, faktor biaya dan tidak adanya konseling dan edukasi. Pemerintah harus mendorong kebijakan medik dalam penanganan pasien gagal ginjal kronik yang komprehensif, di pelayanan primer, skunder dan tertier. Untuk tercapainya kebijakan medik tersebut pemerintah harus menyediakan tenaga yang kompeten, sarana dan prasarana pendukung, standar dan protap yang dibutuhkan untuk masing-masing level pelayanan.
Infused Oyster Mushroom (Pleurotus ostreatus) Inhibits Glucose Absorption through Intestinal Mucosal Membrane in Wistar Rats Eko Fuji Ariyanto; Muhammad Nurhalim Shahib; Abdullah Firmansah Wargahadibrata
International Journal of Integrated Health Sciences Vol 6, No 2 (2018)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.482 KB) | DOI: 10.15850/1354

Abstract

Objective: To observe the effects of infused oyster mushroom (latin name: Pleurotus ostreatus) on glucose absorption through the intestinal mucosal membrane in Wistar rats.Methods: This study used experimental design. Subjects of this study were six Wistar rats which were randomly divided into two groups; three rats were used for the analysis of the most effective time for glucose absorption and the other three were used to evaluate the effect of infused oyster mushroom on glucose absorption. The intestine of subjects was connected to in situ perfusion machine. Control glucose solution (Ctr) or glucose solution containing 5% (Oys 5%), 10% (Oys 10%), and 20% (Oys 20%) infused oyster mushroom was added into the perfusion machine and flowed back and forth in the lumen of intestine subsequently, accompanied by washing procedures using 0.9% NaCl between treatments. The glucose concentration was determined using deproteinization method followed by absorbance measurement. Student’s t test was performed to analyze the difference between groups.Results: The level of glucose absorbed through intestinal mucosal membrane in control glucose solution and glucose solution with different concentrations of infused oyster mushroom, i.e. 5%, 10% or 20%, was 25.19+3.4 mg/dL, 16.27+0.86 mg/dL, 13.22+1.58 mg/dL and 10.03+1.25 mg/dL, respectively. Student’s t test showed significant differences between groups; Ctr and Oys 5% (p=0.03), Ctr and Oys 10% (p=0.02), and Ctr and Oys 20% (p=0.007).Conclusion: Infused oyster mushroom inhibits glucose absorption through intestinal mucosal membrane in Wistar rats.Keywords: Oyster mushroom (Pleurotus ostreatus), glucose absorption, intestine DOI: 10.15850/ijihs.v6n2.1354
Generating Paclitaxel-Resistant in Cervical Cancer HeLa Cell Line Muhammad Hasan Bashari; Fachreza Aryo Damara; Isna Nisrina Hardani; Gita Widya Pradini; Tenny Putri; Eko Fuji Ariyanto
Indonesian Journal of Cancer Chemoprevention Vol 11, No 2 (2020)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev11iss2pp90-96

Abstract

Cervical cancer is one of the most leading causes of women death. Currently, paclitaxel is still one of the main therapeutic regimens for cervical cancer patients. However, some patients developed to be paclitaxel-resistant. Hence, studies to find out the novel strategies to resolve this problem are important. Generating resistant cancer cell lines can be utilized as the potent tool to evaluate the efficacy of any therapeutic agent toward cancer drug-resistant problems. Current studies describing the methods to establish chemoresistance are lacking. Moreover, study in Indonesia conducting chemoresistance in cell line is limited. This study was aimed to elaborate the characteristics of HeLa cells during generation of paclitaxel-resistant cervical cancer cells. The parental HeLa cells were exposed to an escalating concentration of paclitaxel for a long time period. Subsequently, cells were divided into two groups for the evaluation of resistance characteristics. The values of inhibitory concentration 50 (IC50) and inhibitory concentration 90 (IC90) were analyzed using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Our data showed that the longer exposing periods of paclitaxel, the higher IC50 and IC90 values of HeLa cells are. IC90 of paclitaxel in HeLa Pac RB was increased from 69 pM, 440 pM, 2,561 pM and 10,337 pM on 0th, 1st, 2nd, 3rd and 4th months, respectively. Interestingly, the resistant cells were recovered to be paclitaxel-sensitive when they were not being continuously exposed to paclitaxel. In addition, the paclitaxel resistant cells become less sensitive against 5-FU but not doxorubicin, cisplatin and etoposide. We were able to generate cervical cancer HeLa paclitaxel-resistant cell line. These cell line could potentially be utilized for further studies in order to understand the molecular mechanisms of drug resistance in cervical cancer and as a tool for cancer drug discovery.Keywords: cervical cancer, drug resistant cell line, paclitaxel resistant cells, stepwise escalating concentration.
CORRELATION BETWEEN WEEKLY FORMATIVE AND SUMMATIVE ASSESSMENT OF MEDICAL STUDENTS IN MULTIDISCIPLINARY EXAMINATION AND ORAL EXAMINATION REPRODUCTIVE SYSTEM BLOCK Nur Ramadani Meliani Syukri; Yuni Susanti Pratiwi; Eko Fuji Ariyanto; Muhammad Hasan Bashari; Achadiyani Achadiyani; Mohammad Ghozali; Putri Halleyana Rahman
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 10, No 3 (2021): November
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpki.62850

Abstract

Background: The impact of weekly formative assessments on the summative performance of undergraduate medical students is not widely studied. The formative assessment began to be applied in the Undergraduate Medical Study Program, Faculty of Medicine, Padjadjaran University (Program Studi Kedokteran Fakultas Kedokteran Universitas Padjadjaran or PSK FK Unpad) in early 2020. This study aims to determine whether there is a correlation between weekly formative and summative assessment in Multidisciplinary Examination (MDE) and Structured Oral Examination (SOE).Methods: This study was conducted using a numerical correlative bivariate analysis with a cross-sectional design. The data used in this study was weekly formative assessment scores, MDE and SOE scores of Reproductive System (RPS) I and II of 138 medical student batch 2017. Results: Rank-Spearman test shows there was no significant correlation between the formative test scores and the MDE RPS I score (p>0.05) and the SOE score (p>0.05). Meanwhile, the MDE RPS II score with the formative test was found to be a significant correlation (p<0.05) and positively correlated (r=+0.211).Conclusion: This study shows that the use of weekly formative assessments might contribute to the students' achievement of MDE scores. Weekly formative assessments can be integrated into the curriculum of pre-clinical medical programs to improve student summative performance.
THE RELATIONSHIP BETWEEN TYPES OF ADMISSION SELECTION AND ACADEMIC ACHIEVEMENT IN THE FIRST-YEAR STUDENTS DURING THE TRANSITION TO DISTANCE LEARNING DUE TO COVID-19 PANDEMIC Nikla Sekar Salsabila; Kurnia Wahyudi; Yuni Susanti Pratiwi; Eko Fuji Ariyanto; Achadiyani Achadiyani
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 11, No 1 (2022): Maret
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpki.64464

Abstract

Background: In 2019, there were three admission pathways in the Faculty of Medicine Universitas Padjadjaran (FMUP), which were SNMPTN (Seleksi Nasional Masuk Perguruan Tinggi Negeri), SBMPTN (Seleksi Bersama Masuk Perguruan Tinggi Negeri), and Seleksi Mandiri Universitas Padjadjaran (SMUP). SNMPTN was based on previous academic achievements in senior high school. SBMPTN was held through a highly competitive national examination, while SMUP was conducted through the test held by Universitas Padjadjaran. In their first year, batch 2019 students had to adapt to college life, and also to the transition into distance learning due to the COVID-19 pandemic. This study aimed to know whether types of student admission selection contribute to academic achievements of the first-year students in the Hemato-immunology system (HIS), where the learning process was held during the transition into distance learning.Methods: This study compared the academic achievement of first-year students (batch 2019) on the second semester’s second half, using their HIS Multidisciplinary Examination (MDE) scores, for three selection methods. The data were 256 MDE scores of those students. Statistical analysis was performed using One Way ANOVA test, followed by Post-hoc Tukey test.Results: There was a statistically significant difference of HIS MDE scores for each selection method. The highest mean was by SNMPTN group (68.29+10.44), followed by SBMPTN group (64.90+11.29) and SMUP group (63.25+9.95).Conclusion: Students of SNMPTN group, who were selected based on previous academic achievements in senior high school, had higher HIS MDE scores than the other groups. 
Upaya Peningkatan Pengetahuan Masyarakat tentang Pencegahan Stunting melalui Pendidikan Komunitas Interaktif Eko Fuji Ariyanto; Bianda Putri Ramadhani; David Firmansyah; Rizkiani Tri Ramdani; Muhammad Abian; Lauditta Nurseptia Prihandhini; Anisha Salmah Azhar; Fahmi Zacki Mubarak; Dwi Wahyudha Wira; Rima Destya Triatin
Jurnal Pengabdian Pada Masyarakat Vol 4 No 3 (2019)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (628.665 KB) | DOI: 10.30653/002.201943.153

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EFFORTS TO IMPROVE COMMUNITY KNOWLEDGE ABOUT STUNTING PREVENTION THROUGH EDUCATION OF INTERACTIVE COMMUNITIES. Stunting is one of the main health problems in Indonesia, including West Java Province. Some factors can cause this problem, especially inadequate nutrition. Community education regarding stunting prevention were delivered through interactive health promotion in Sayang village, Jatinangor sub-district, Sumedang district, West Java. The audiences were purposively selected, including village cadres and mothers with under-five children. The materials delivered in this program included practical information about how to prepare balanced nutrition for babies and under-five children. Seventy two audiences actively participated in this program. The enthusiasm of the audiences was very high, shown by very interactive discussion during this program. The participants could answer most of the questions given by the speakers. Community education through interactive health promotion which involved cadres and purposively selected group as the audiences was a reliable method to increase knowledge and awareness of the community for stunting prevention.
POTENSI METABOLIT SEKUNDER DARI JAMUR YANG BERASOSIASI DENGAN SPONS LAUT SEBAGAI SUMBER SENYAWA ANTIKANKER Iqbal Anugrah Fitriyanto; Muhammad Hasan Bashari; Eko Fuji Ariyanto
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 4 No 5 (2021): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.825 KB)

Abstract

Beban kesehatan yang ditimbulkan akibat kanker terus meningkat seiring dengan morbiditasdan mortalitas yang nyata baik di negara maju maupun berkembang. Jamur laut merupakansumber metabolit sekunder yang berguna untuk tujuan penemuan obat. Sejumlah senyawametabolit yang dihasilkan oleh jamur laut telah menunjukkan efek antikanker kuat. MetabolitCytochalasin K yang dihasilkan jamur Arthrinium arundinis ZSDS1-F3 yang diisolasi darispons laut Phakellia fusca memiliki aktivitas sitotoksik terhadap lini sel K562, A549, Huh-7,H1975, HL60, Hela, dan MOLT-4. Metabolit Disydonol A yang diproduksi oleh jamurAspergillus sp. yang diisolasi dari spons laut Xestospongia testudinaria memiliki aktivitassitotoksik terhadap lini sel HepG-2 and Caski. Metabolit Marilines A1 dan A2 yangdihasilkan oleh jamur Stachylidium sp. yang diisolasi dari spons laut Callyspongia cf. C.flammea memiliki aktivitas sitotoksik terhadap lini sel HLE. Dalam ulasan ini, kamimenyoroti beberapa jamur yang berasal dari laut dengan metabolit yang dapat memodulasiaktivitas beberapa enzim yang berperan penting pada pertumbuhan dan metastasis sel tumor. DOI : https://doi.org/10.35990/mk.v4n5.p553-566
Comparative of Neutrophils and Lymphocyte Ratio for Dairy Cattle in Prepartum and Postpartum Condition Diza Raudya Tuzzahra; Eko Fuji Ariyanto; Septiyani -; Aziiz Mardanarian Rosdianto
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.67404

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There is a transition period that dairy cattle always pass through in their reproduction cycle, making them more stressed. Stress can simply be identified by the ratio of neutrophil (N) and lymphocyte (L) at the haematologic state of dairy cattle. The purpose of this study is to evaluate stress conditions in dairy cattle’s transition period based on neutrophil and lymphocyte ratio. Twenty samples of dairy cattle’s blood in prepartum and postpartum condition are being analyzed with a hematology analyzer. The result of this study shows that the average of neutrophil has increased insignificantly from prepartum to postpartum period as 4.14 103/µL ± 1.51 to 4.27 103/µL ± 2.08 or 1.03% with a normal interval of neutrophil is 1.7-6.0 103/µL. However, the average of lymphocytes decreased insignificantly from 3.23 103/µL ± 1.13 to 3.03 103/µL ± 1.23 or 0.93% with a normal interval of lymphocyte is 1.8-8.1 103/µL. Then, the average of neutrophil and lymphocyte ratio has increased insignificantly from 1.35 103/µL ± 0.46 to 1.47 103/µL ± 0.60 or 1.09%. Based on this result, the ratio of neutrophils per lymphocyte still in the normal range. In the transition period, only a few dairy cattle have a stressful condition. The stress condition in dairy cattle can be identified by increasing neutrophil (neutrophilia) and reducing lymphocyte (lymphocytopenia).
EFEK EKSTRAK ETANOL KULIT BUAH NAGA MERAH TERHADAP FAGOSITOSIS BAKTERI OLEH MONOSIT Muhammad Raihan Ramdhan; Nurjanah Sriyanti; Sri Betha Putri; Fauzia Rahma Cahyani; Iis Herawati; Eko Fuji Ariyanto
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 5 No 4 (2022): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Prevalensi penyakit infeksi di Indonesia masih tinggi, di antaranya Infeksi Saluran Pernapasan Atas (ISPA) 9,3% dan diare 8%. Penyakit infeksi menjadi penyebab kematian kedua tertinggi pada kelompok usia > 55 tahun setelah penyakit pada sistem peredaran darah. Buah naga merah (Hylocereus lemairei (Hook.) Britton & Rose) memiliki manfaat bagi kesehatan, namun saat ini kulit buah naga merah belum dimanfaatkan. Ekstrak etanol kulit buah naga juga telah diketahui berpotensi sebagai antioksidan yang tinggi. Penelitian ini bertujuan untuk mengetahui efek ekstrak kulit buah naga merah terhadap fagositosis bakteri oleh monosit. Penelitian dilakukan dengan tahapan: pembuatan ekstrak etanol kulit buah naga merah, pengambilan sampel darah manusia dan isolasi peripheral blood mononuclear cells (PBMC) dan monosit, pemberian ekstrak etanol kulit buah naga merah ke dalam sumuran monosit, pengujian bakteri uji menggunakan Staphylococcus aureus ATCC 29213 dan Escherichia coli ATCC 25922, pengambilan dan pengkulturan bakteri uji, dan penghitungan jumlah koloni bakteri yang tumbuh. Hasil penelitian menunjukkan bahwa pemberian ekstrak kulit buah naga merah dengan konsentrasi 10 μg/ml menghasilkan efek yang paling besar dalam meningkatkan jumlah S. aureus yang difagositosis, yaitu sebesar 4,31% dan ekstrak dengan konsentrasi 5 μg/ml meningkatkan jumlah E. coli yang difagositosis sebesar 1,26%. Analisis statistik tidak menunjukkan perbedaan yang bermakna antargrup (p > 0,05). Penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit buah naga merah tidak memberikan efek yang bermakna terhadap fagositosis bakteri oleh monosit. Kata kunci : Bakteri, ekstrak kulit buah naga, fagositosis, immunomodulator, monosit DOI : 10.35990/mk.v5n4.p412-423