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Molecular Detections and Resistance Response of Six Rice Varieties to Tungroviruses from South Sulawesi Saipul Abbas; Sri Sulandari; Sedyo Hartono; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.47355

Abstract

The suspected rice virus is found in the field, namely the tungrovirus which is transmitted by green leafhoppers (Nephotettix virescens). The study aimed to detect the tungrovirus molecularly and examine the resistance response of six rice varieties from the transmission of tungrovirus samples from South Sulawesi on a greenhouse scale. Based on the results of molecular detection with RTSV PCR of the double infected sample with DNA bands 1115 bp and RTBV of around 430 bp, Sidrap, and Maros samples were infected by 430 bp size RTBV, while Wajo sample was not detected by both viruses. The results of RTBV sequence analysis showed that the grouping of Sidrap was still one group with Maros and Pinrang samples and different from the group of samples from Malaysia, Thailand, and Philippines. While the grouping of RTSV shows that Pinrang samples are still one group with samples from Bali, Subang, and different from those of the Philippines, India, and Malaysia. The results of transmission in the greenhouse on six rice varieties (TN1, Ciherang, Mekongga, Tukad Unda, Inpari 36, Inpari 37) showed different plant resistance responses such as susceptible, moderately resistant, and resistant reactions based on the amount of disease intensity caused. Varieties that are classified as susceptible are TN1 and Ciherang varieties, moderately resistant, namely Mekongga and Tukad Unda varieties, and resistant varieties namely Inpari 36 and Inpari 37 varieties.
Population Fluctuations of Scirpophaga innotata and Nilaparvata lugens In Various Varieties and Growing Age of Rice Plants Saipul Abbas; Itji Diana Daud; Sri Nur Aminah Ngatimin
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.4645

Abstract

Stem borer (Scirpophaga innotata) and brown planthopper (Nilaparvata lugens) are known as important pests in rice plants that attack plants from the nursery until just before harvest. Efforts to introduce new high-yielding varieties need to be carried out to provide farmers with references to varieties that are resistant or not to pest populations in the field. This study aims to look at the population levels of stem borer and brown planthopper pests at different planting ages for several test varieties. The method used was a randomized block design with 6 varietal treatments (IR14, IR15, IR16, IR18, IR19, IR20) and 3 tests with sampling intervals every week until 77 days after planting (HST). Data were analyzed using the Analysis of Variance (Anova) method and the Least Significant Difference Test (LSD). The results showed that the highest population of stem borers was found in plants aged 63 HST, namely 8 individuals/clump, while the lowest average population was found in plants aged 49 HST, namely 1 individual/clump. While the highest brown planthopper population was found at 77 HST, namely 10 individuals/clump, for the lowest population average at 63 HST, namely 4 individuals/clump. Populations of stem borer and brown planthopper in fact preferred rice variety IR14 with the highest population, while rice variety IR18 had the lowest population.
Isolation And Characterization Of Phosphate Solving Bacteria From Swamp Soil With High Levels Of Acidity Galang Indra Jaya; Sri Nuryani Hidayah Utami; Jaka Widada; Wahida Annisa Yusuf; Saipul Abbas; Nur Fatturahman Ridwan; Amir Noviyanto
Jurnal Pertanian Vol. 14 No. 2 (2023): Oktober
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jp.v14i2.9932

Abstract

Phosphate solubilizing bacteria (PSB) are one of the microbes that play an important role in soil and plant cycles. Phosphate (P) is a very important macronutrient for plants. In soil, most of the P element is found to be unavailable to plants because it is fixed by Ca, Al or Fe. This research aims to identify BPF in acid soil which has the potential to dissolve phosphate elements. The method used in this research is the isolation of BPF from acid soil using the National Botanical Research Institute's Phosphate (NBRIP) medium, phosphate dissolution index test and UV Visual. Soils from overflow type C (TLC) swamps have higher diversity compared to TLB soils. The abundance of BPF in TLC soil was higher (5,4 107 CFU per gram) compared to soil from overflow zone B (TLB) (2,9 107 CFU per gram) because TLC soil had a lower acidity level than TLB. There were 55 BPF isolates obtained from these two types of soil. Two isolates (TLB1 and TLB2) had a better phosphate solubilization index and all potential isolates that were extracted and subjected to gDNA amplification showed a DNA band at 1330-1500 bp.
Pelatihan Pembuatan CendawanTrichoderma sp. dalam Mengendalikan Penyakit Akar Putih pada Perkebunan Karet di Desa Binturu, Kalimantan Selatan Muslimin Sepe; Helda Orbani Rosa; Salamiah; Saipul Abbas; Yusriadi Marsuni; Muhammad Indar Pramudi; Dewi Fitriyanti; Devaliana Catria Fikasari; Vera Trichoderma
Jalujur: Jurnal Pengabdian Masyarakat Vol. 2 No. 2 (2023): Desember
Publisher : UIN Antasari Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18592/jalujur.v2i2.11094

Abstract

Penyakit penting yang menyerang tanaman karet yaitu cendawan akar putih, kering alur sadap, gugur daun Corynospora, Colletotrichum dan Oidium. Pohon karet yang terserang penyakit akan mengalami pengguguran daun sehingga pohon karet enggan mengeluarkan getahnya yang membuat penghasilan petani menurun drastis. Produksi dan kualitas karet dapat ditingkatkan dan dipertahankan dengan cara memperhatikan teknik budidaya dan pengendalian organisme pengganggu tumbuhan dengan tepat. Salah satu alternatif menekan populasi OPT dengan memanfaatkan mikroorganisme berguna seperti cendawan Trichoderma sp. Metode PkM yaitu pemberdayaan partisipatif berupa penyuluhan, pelatihan dan pendampingan yang melibatkan peran serta mitra yaitu petani Desa Binturu, Kecamatan Kelua, Kabupaten Tabalong secara aktif mulai dari perencanaan, pelaksanaan, monitoring dan evaluasi. Hasil pengabdian kepada masyarakat menunjukkan sikap antusias dari kelompok tani dalam hal pelatihan pembuatan Trichoderma sp. dan cara pengaplikasiannya di Desa Binturu Kecamatan Kelua, Kabupaten Tabalong, Kalimantan Selatan.