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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Journal of Aquaculture Management and Technology Omni-Akuatika Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Jurnal Litbang Provinsi Jawa Tengah
- Desrina, -
Department Of Aquaculture, Fisheries And Marine Science Faculty, Diponegoro University, Tembalang, Semarang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Public Health Social Sciences Other

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Journal : Omni-Akuatika

 1 
The Diversity of Causative Agent Associated With Bacterial Diseases on Catfish (Clarias gariepinus) with Molecular Based from Demak, Indonesia Sarjito Sarjito; A. Harjuno Condro Haditomo; Desrina Desrina; Restiana Ariyati; S. Budi Prayitno
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.723 KB) | DOI: 10.20884/1.oa.2018.14.2.553

Abstract

Bacterial diseases is frequently occur in catfish culture. The aim of this research was to find out the diversity of causative agent associated with bacterial diseases in catfish based on 16S rDNA gene sequences. The combination between exploratory in the field and experiment, method were applied. Seventeen isolates (D01–D17) were gained from kidney and external wound of moribound catfish with NA and GSP medium that were collected from fish pond of Demak Regency, Indonesia. Based on the postulat results showed that four isolates (D07, D10, D11 and  D14) that  were  caused  10–55% of fishes get sick  and  0–30%  fishes mortal.  On the other hand, there were 13 isolates do not cause both sick and mortality of fish.  On the basis of sequence 16S rDNA analysis, the result showed that D07, D10, D11 and D14 were closely related to Aeromonas caviae (96%), Aeromonas veronii (97%.), Plesiomonas shigelloides (97%) and Pseudomonas putida (96%) respectively. The sensitivity test result indicated that these causative agents have not sensitively to some fish drugs test.
Molecular Characteristics of Indonesian Isolate Enterocytozoon hepatopenaei Based on Sequence Analysis of 18S rRNA Genes Yohanes Kristiawan Artanto; Slamet Budi Prayitno; Sarjito Sarjito; Desrina Desrina; Alfabetian Harjuna Condro Haditomo
Journal Omni-Akuatika Vol 15, No 1 (2019): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (12.995 KB) | DOI: 10.20884/1.oa.2019.15.1.694

Abstract

ABSTRACT Enterocytozoon hepatopenaei (EHP) infection have been reported as an obstacle of whiteleg shrimp (Penaeus vannamei) culture in Indonesia. However, understanding of the molecular characteristics of EHP species in Indonesia is not widely known. The aims of this study were to determine the identity and characters of DNA, and their phylogeny of EHP species from several different locations in Indonesia with specific references to 18S rRNA gene. The EHPs were collected from cultured P.vannamei from Lampung, Pangandaran, Sidoarjo, Banyuwangi, Probolinggo, Blitar, Makassar, and Lombok. Thirteen (13) samples were randomly selected to explore their gene characters through 18S rRNA gene sequencing. The primers used were EHP_F and EHP_1R. Parameter observed were DNA sequencing, nucleotide sequence homology with related available genes in the Gen Bank database, multiple sequence alignment, and reconstruction of genetic relationship trees. DNA sequence homology analysis showed that all samples had 99.89-100% similarity to Indian Enterocytozoon hepatopenaei (Accession Number MH259890.1 and MH260592.1). The alignment results illustrated that all EHP sequences of Indonesian isolates were 100% identical each other. The phylogenetic tree topology provided information that all sample accessions were in the same clade and spread evenly. The conclusion were that the Indonesian EHP species were identical (100%) and it could be said that they were genetically homogeneous. Keywords: Enterocytozoon hepatopenaei, 18S rRNA gene, Indonesia.
Effectiveness of Filter Media Compositions on Water Quality, Growth and Survival Rate of Tilapia (Oreochromis niloticus) Cultured in Recirculation System Mamluatul Lailiyah; Dicky Harwanto; Desrina Desrina
Journal Omni-Akuatika Vol 19, No 1 (2023): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2023.19.1.963

Abstract

Market demand for tilapia (Oreochromis niloticus) is increasing every year. This has encouraged aquaculture companies to apply intensive cultivation systems. However, these applications run the risk of increasing waste and decreasing water quality. One strategy to overcome those problems of water quality is to use recirculation aquaculture system. This study aims to determine the effectiveness of filter media composition on water quality, survival rate, and tilapia growth. The average body weight of the fish used was 3.84 ± 0.05 g per individual. A completely random experiment design with three treatments and three replications were applied. The treatments were combination of several filter media with different compositions, namely A (Dacron + 75% nets + 25% sand), B (Dacron + 50% nets + 50% sand) and C (Dacron + 25% nets + 75% sand).The water quality variables measured were pH, DO, temperature, ammonia, and VTR (Volumetric TAN Removal). The survival rate (SR), relative growth rate (RGR). and total amount feed fed (TFF) of fish were also measured. The results showed that the composition of the filter had a significant effect (P<0.05) between treatment. The best VTR was showed in Treatment C with value 54.65 gm-3d-1.Keywords: Tilapia, recirculation, dacron, nets, sand
Co-Authors A. Harjuno Condro Haditomo Alfabetian Condro Haditomo Alfabetian Harjuna Condro Haditomo Alfabetian Harjuno Condro Haditomo Ambariyanto Ambariyanto Arief Taslihan Arief Taslihan Budhi Kuncoro Jati Condro Haditomo Dicky Harwanto Fifiana Zulaekah Ita Widowati Kahan Dwi Supardi Mamluatul Lailiyah Mita Umiliana, Mita Monica Nanda Nida Qolbi Salma Rochani Peppy Dewi Fitriyanti Restiana Ariyati Restiana Wisnu Ariyati Rosa Amalia S B Prayitno S. Budi Prayitno Sarjito - Sekar Mentari Putri, Sekar Mentari Slamet Budi Prayitno Sulisyaningrum Sulisyaningrum Suryaningrum Susiani Tristiana Yuniarti Wiji Utami, Wiji Yohanes Kristiawan Artanto Yuni Karnisa Yunia Dharmastuti Widyaningsih