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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Meditory : The Journal of Medical Laboratory JFM (Jurnal Farmasi Malahayati) JURNAL ILMIAH KESEHATAN SANDI HUSADA Muhammadiyah Medical Journal Jurnal Pengabdian kepada Masyarakat Wahana Usada Jurnal Yoga Dan Kesehatan Journal Pharmactive
Ni Wayan Rika Kumara Dewi
Institut Teknologi Dan Kesehatan Bintang Persada
Religion Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry)

 1 
ISOLASI DAN IDENTIFIKASI SENYAWA ANTIOKSIDAN GOLONGAN FLAVONOID DARI EKSTRAK ETIL ASETAT DAUN PRANAJIWA (Euchresta horsfieldii Lesch Benn.) Ni Wayan Rika Kumara Dewi; I Wayan Gunawan; Ni Made Puspawati
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 5 No 1 (2017)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.127 KB) | DOI: 10.24843/CK.2017.v05.i01.p04

Abstract

ABSTRAK: Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dan mengidentifikasi senyawa golongan flavonoid yang terkandung dalam ekstrak etil asetat daun pranajiwa (Euchresta horsfieldii Lesch Benn.). Uji aktivitas antioksidan dilakukan secara invitro dengan metode DPPH (1,1-diphenyl-2-pycrylhidrazyl). Teknik pemisahan dilakukan dengan kromatografi kolom dan diidentifikasi dengan spektrofotometer FTIR dan UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak etil asetat memiliki aktivitas antioksidan dengan nilai IC50 sebesar 393,95 µg/mL dan kadar total flavonoid sebesar 6619,72 mg QE/100g atau 6,62 % QE. Pemisahan dan pemurnian ekstrak etil asetat menggunakan fase diam silika gel 60 dan fase gerak n-heksana:kloroform: etanol (20:1:1) yang menghasilkan isolat aktif yang positif flavonoid (isolat FE) yang diduga merupakan senyawa flavonoid golongan flavonol yaitu 3,5,7,3’,4’-pentahidroksi flavonol. ABSTRACT: The present study was conducted to determine antioxidant activity and to identify the flavonoid active compounds from ethyl acetate extracts of Pranajiwa leaves. In vitro antioxidant activity was carried out using DPPH (1,1-diphenyl-2-pycrylhidrazyl) method. The separation of the ethyl acetate extracts was conducted by column chromatography and the identification by using FTIR and UV-Vis spectrophotometer. The results showed that ethyl acetate extract has antioxidant activity with IC50 value of 393,95 µg/mL and the total flavonoid of 6619,72 mg QE /100g or 6,62% QE. The separation and purification of ethyl acetate extract was chromatography column on silica gel 60 and the solvent n-hexane:chloroform:ethanol (20:1:1) based on UV-Vis and Infrared spectra positive flavonoid isolate (isolates FE) was tentaviely identified as 3,5,7,3',4'-pentadihydroxy flavonols.
Co-Authors Andi Udin Saransi Aprilia Rakhmawati Burhannuddin Gede Terima Yasa Heri Setiyo Bekti I Wayan Gede Gunawan Iwan Saka Nugraha Ketut Lilik Arwati Made Dwike Swari Santi Made Prita Artika Ni Komang Omik Trianita Udiana Ni Luh Gde Mona Monika Ni Made Puspawati Ni Nyoman Astika Dewi Ni Putu Ditya Prabandari Dewi Ni Putu Gita Puspita Sari Ni Putu Yulia Rahma Dewi Nur Habibah Puspita Sari Dyah Ratna Ayu Putu Yudha Ugrasena Rinawati, Luh Putu