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All Journal Jurnal Teknik ITS IPTEK Journal of Proceedings Series IPTEK The Journal for Technology and Science Journal of Engineering and Technological Sciences International Conference on Coastal and Delta Areas Adi Widya : Jurnal Pengabdian Masyarakat IJOCE (International Journal of Offshore and Coastal Engineering) Jurnal Purifikasi International Journal of Marine Engineering Innovation and Research Jurnal Kadesi : Jurnal Teologi dan Pendidikan Agama Kristen Jurnal Pengabdian Pada Masyarakat Sewagati
Herman Pratikno, Herman
Institut Teknologi Sepuluh Nopember
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Journal : Journal of Engineering and Technological Sciences

 1 
Isolation and Screening of Diesel Degrading Bacteria from Ship Dismantling Facility at Tanjungjati, Madura, Indonesia Titah, Harmin Sulistiyaning; Pratikno, Herman; Moesriati, Atiek; Imron, Muhammad Fauzul; Putera, Rizky Islami
Journal of Engineering and Technological Sciences Vol 50, No 1 (2018)
Publisher : ITB Journal Publisher, LPPM ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.282 KB) | DOI: 10.5614/j.eng.technol.sci.2018.50.1.7

Abstract

The ship dismantling industry is a cause of contamination of the environment by diesel. The objectives of this study were to isolate and screen diesel degrading bacteria from diesel contaminated areas. Diesel contaminated seawater and soil samples were collected from a ship dismantling facility at Tanjungjati, Madura, Indonesia. Isolation was conducted with an aseptic technique and growing the mixture culture was carried out based on the pour plate method. After 24 h of incubation, thirteen bacteria strains were isolated from diesel contaminated seawater and soil samples from the area of study. The isolated bacteria were identified based on morphological characterization. Mostly gram positive bacteria were found. The isolated bacteria were screened by using nutrient agar medium containing various diesel concentrations (0%, 5%, 10%, and 15% (v/v)). The result of the screening test showed that the bacteria coded EL and CT displayed the best resistance and highest growth in diesel polluted medium. It was shown that both of them potentially have a higher capability of utilizing diesel as carbon and energy source than the others.
Isolation and Screening of Diesel Degrading Bacteria from Ship Dismantling Facility at Tanjungjati, Madura, Indonesia Harmin Sulistiyaning Titah; Herman Pratikno; Atiek Moesriati; Muhammad Fauzul Imron; Rizky Islami Putera
Journal of Engineering and Technological Sciences Vol. 50 No. 1 (2018)
Publisher : Institute for Research and Community Services, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.eng.technol.sci.2018.50.1.7

Abstract

The ship dismantling industry is a cause of contamination of the environment by diesel. The objectives of this study were to isolate and screen diesel degrading bacteria from diesel contaminated areas. Diesel contaminated seawater and soil samples were collected from a ship dismantling facility at Tanjungjati, Madura, Indonesia. Isolation was conducted with an aseptic technique and growing the mixture culture was carried out based on the pour plate method. After 24 h of incubation, thirteen bacteria strains were isolated from diesel contaminated seawater and soil samples from the area of study. The isolated bacteria were identified based on morphological characterization. Mostly gram positive bacteria were found. The isolated bacteria were screened by using nutrient agar medium containing various diesel concentrations (0%, 5%, 10%, and 15% (v/v)). The result of the screening test showed that the bacteria coded EL and CT displayed the best resistance and highest growth in diesel polluted medium. It was shown that both of them potentially have a higher capability of utilizing diesel as carbon and energy source than the others.
Co-Authors Abdullah Husin Baredwan Agung Purniawan Andrea Novia Samiyono Andry Febrianto Ardi Prasetia Yanuar Arifin Amanto Putra Atha Fazadima Atiek Moesriati Atiek Moesriati Bayu Mahardika Bima Satriya Willy Harsa Bintang Respati Dwi Harnani Carolus Trijatmiko Nugroho Dirta Marina Chamelia Dwi Maharani Aisyah Dwi Purnomo H, Zuhud Ubaidillah & Miftakh D.S Faruq Abdullah Febrian Ramadhana Sastradimaja Felita Widya Hapsari Gilang Rezha Mahardika Hafizh Abiyyuda Hafizh Abiyyuda Handayanu Handayanu Handayanu Handayanu Harmin Sulistyaning Titah Harmin Sulistyaning Titah Hasan Ikhwani Hasan Ikhwani Hasan Ikhwani Hasan Ikhwani Hasan Ikhwani Inastika Nabilah Inaya Krista Pradyptia Madi Madi Madi Mahmud Mustain Mahmud Mustain Maria Anityasari Miftakh D.S, Dwi Purnomo H, Zuhud Ubaidillah & Moch. Zaed Yuliadi Muhammad Fauzul Imron Muhammad Ichsan Rosidin Yuniarto Muhammad Makki Romadhoni muhammad Rizkqy Jafa Muhammad Rizqy Jafa Muhammad Sultanul Azdkar Nugraha Adhi Pahlawan Nur Syahroni Pandu Damay Putra Putera, Rizky Islami Rahmadhani Tasya Rahman Ray Adam Baihaqi Raymond Habinsaran Aruan Rizkiyah Ramadani Rizky Islami Putera Rizky Islami Putera Rochani, Imam Rudi Walujo Prastianto Rudi Walujo Prastianto Safira Dwi Anggraeni Sholihin Sholihin Sholihin Sujantoko Sujantoko Suparno Suparno Taufik Fajar Nugroho Tin Rachmatullah Tin Rachmatullah Titah, Harmin Sulistiyaning Totok Yulianto Wimala Lalitya Dhanista Wimala Lalitya Dhanista Wimala Lalitya Dhanistha Yoyok Setyo H Yoyok Setyo Hadiwidodo Yoyok Setyo Hadiwidodo Yoyok Setyo Hadiwidodo Yudha Prasetyawan Yudiardana Tridantoko Susarno