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Biotransformasi Tartrazin oleh Bakteri Usus Manusia Batubara, Melvariani Syari; Ginting, Nurmaini
BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Vol 4, No 2 (2018): Februari
Publisher : Program Studi Biologi, Fakultas Biologi Universitas Medan Area

The aimed of this research was to investigated human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri), to investigated dose of tartrazine, and to investigated biotransformation of tartrazine by human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri). The method of this research was experiment with significant correlation to investigated biotransformation of tartrazine by human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri). This study is primarily focussed on made bacteria medium, made standart inoculums of bacteria, and evaluation for activity of human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri). The results obtained were attributed to the human intestinal bacteria was used in this research was biotransformated tartrazine. From the results that had been increase dose of tartrazine correlation with increase biotransformation by human intestinal bacteria. Highest dose of tartrazine was 15 mg, and highest biotransformation by Vibrio fischeri, which rate of zone decolourisation diameter was 1,22 cm, and rate of zone decolourisation was 1,44 cm2. Statistically showed significantly in correlation dose of tartrazine with rate of zone decolourisation (0,001 < P < 0,05), but statistically showed non significantly in correlation bacteria with rate of zone decolourisation (0,05 < P < 0,268).

Hasil Uji Coba Video Pembelajaran Mata Kuliah Kultur Jaringan Berbasis Masalah pada Dosen dan Mahasiswa Program Studi Pendidikan Biologi UMTS Batubara, Melvariani Syari
Jurnal Pendidikan Biologi Vol 6, No 2 (2017): Jurnal Pendidikan Biologi
Publisher : Magister Pendidikan Biologi, Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Penelitian ini bertujuan untuk mengembangkan media video pembelajaran kultur jaringan berbasis masalah. Penelitian pengembangan ini menggunakan model Borg dan Gall melalui 7 tahapan yaitu: (1) Pengumpulan data penelitian berupa nilai hasil belajar mahasiswa mata kuliah kultur jaringan, observasi, dan wawancara kepada mahasiswa yang mengikuti mata kuliah kultur jaringan dan dosen pengampu mata kuliah kultur jaringan, (2) Perencanaan berupa membuat sintak pembelajaran berbasis masalah (PBL) dan membuat skenario setiap materi kultur jaringan, (3) Pengembangan produk awal berupa video pembelajaran kultur jaringan berbasis masalah, (4) Uji coba lapangan produk utama berupa uji teman sejawat, perorangan dan kelompok kecil oleh mahasiswa yang mengikuti mata kuliah kultur jaringan, (5) Revisi produk uji coba lapangan utama, (6) Uji coba lapangan skala luas berupa uji coba lapangan terbatas oleh mahasiswa yang mengikuti mata kuliah kultur jaringan, (7) Revisi produk final hasil uji coba lapangan skala luas. Pengumpulan data dilakukan dengan menggunakan angket. Data dianalisis dengan menggunakan teknik analisis deskriptif. Dari hasil penilaian terhadap video pembelajaran kultur jaringan berbasis masalah yang telah dikembangkan dinilai âlayakâ digunakan dengan persentase rata-rata skor 76,7% oleh teman sejawat dosen. Hasil tanggapan mahasiswa dinilai âlayakâ digunakan, melalui uji coba perorangan dengan persentase rata-rata skor 75,6%, uji coba kelompok kecil 73,8%, dan uji coba lapangan terbatas 82%. Media video pembelajaran kultur jaringan berbasis masalah dikategorikan baik dan â layakâ digunakan pada perkuliahan kultur jaringan.

HUBUNGAN KADAR TARTRAZIN DAN SENG (ZN) DALAM DARAH PADA ANAK PENDERITA DEFISIT PERHATIAN DAN GANGGUAN HIPERAKTIVITAS (ADHD) Batubara, Melvariani Syari; Siregar, Yahwardiah; Rusmarilin, Herla; Soviani, Sri; Febriani, Husnarika
BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Vol 4, No 1 (2017): Agustus
Publisher : Program Studi Biologi, Fakultas Biologi Universitas Medan Area

This research aims to study Tartrazine and Zinc (Zn) status in blood sample of Attention Deficit Hyperactivity Disorder (ADHD) children. Deductive research using the descriptive method through 3 stage, namely : 1. Taken of blood sample, 2. Analysis of Tartrazine status in blood sample was performed by using High Performance Liquid Chromatograpy (HPLC) method, and 3. Analysis of Zinc (Zn) status in blood sample were measurements by using Atomic Adsorbance Spectrophotometry (AAS) method. Data were analyzed using descriptive analysis techniques and Chi Square tests (X2) by Computer programs (SPSS 15). The result of the assessment of Tartrazine status in blood sample of ADHD children that had been was considered statistically highly significant a increase of the assessment of Tartrazine status in blood sample of ADHD children by 130 mg/ml compared with laboratory reference (control) by 28 mg/ml, HA>C** (p<0,001). The result of the assessment of Zinc (Zn) status in blood sample of ADHD children that had been was showed statistically highly significant a reduce of the assessment of Zinc (Zn) status in blood sample of ADHD children by 0,37 Âµg/ml compared with laboratory reference (control) by 0,64-1,18 Âµg/ml, HA<C** (p<0,001). This was considered a reduction and increase in Tartrazine and Zinc (Zn) concentrations induces of the Hyperactivity children by 95%.

PENGARUH PEMBERIAN EKSTRAK DAUN ANDALIMAN (ZANTHOXYLUM ACANTHOPODIUM DC.) TERHADAP HISTOLOGIS OVARIUM MENCIT (MUS MUSCULUS L.) Batubara, Melvariani Syari; Sabri, Emita; Tanjung, Masitta
BIOLINK : Jurnal Biologi Lingkungan Industri Kesehatan Vol 6, No 2 (2020): Februari
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v6i2.2409

Research the effect of andaliman leaf extract on mice ovaries, volume test material using oral gavage needles as much 0.1 ml / 10 g bb 4 days before and after estrus stage and using RAL non-factorial method consisting of control and treatment group. Treatment consisted of factor test material with dose 2500, 5000, 10000, 20000, 40000 ppm. Chemical content of andaliman leaf extract shown from results study are alkaloids, steroids and saponins. Giving treatment of andaliman leaf extract for 9 days on histological data in the number primary, secondary, De Graff, and atretic follicles experienced fluctuating changes, but statistically did not show a significant difference between treatment and control mice groups, while different results were significantly shown in the number of corpus luteum. Parameters diameter of primary follicles, De Graff, and corpus luteum tended to experience changes that fluctuated, but statistically did not show the difference between the treatment and control mice groups, while the diameter of secondary and atretic follicles showed significantly different results. From the results study, administration of andaliman leaf extract affects histological mice ovaries, because it affects oogenesis and ovulation, but fluctuates, possibly due to crude extract, which has not isolated chemical content.

PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN ANDALIMAN (Zanthoxylum acanthopodium DC.) TERHADAP GAMBARAN MORFOLOGI OVARIUM MENCIT (Mus musculus L.) STRAIN DDW. MELVARIANI SYARI BATUBARA; EMITA SABRI; MASITTA TANJUNG
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 1, No 1 (2017): Klorofil: Jurnal Ilmu Biologi dan Terapan
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v1i1.1232

A study on the effect of andaliman (Zanthoxylum acanthopodium DC.) leaves ethanol extract to morphological ovary of mice (Mus musculus L.) strain DDW. has been, and was given orally by gavage on 4 days before estrus and 4 days after estrus with the volume given was 0,1 ml/10 g body weight. The research has been studied at Animal Structure And Physiology Laboratory of Department of Biology in Mathematics And Natural Science Faculty, University ofÂ North Sumatera in Medan since February 2006 until September 2006. The research method used was completely randomized designed non factorial which consist of treatment and control groups. The treatment had one factor that is extract dosages with 2500 ppm (0,25 g/100 ml aquadest), 5000 ppm (0,5 g/100 ml aquadest), 10000 ppm (1,0 g/100 ml aquadest), 20000 ppm (2,0 g/100 ml aquadest), 40000 ppm (4,0 g/100 ml aquadest).The result showed that the chemistry consist of andaliman leaves extract is alkaloid, steroid, and saponin. The effect of andaliman leaves extract on 9 days to morphology ofÂ ovary weight and diameter of mice did effect, but DnMRT showed non significantly effect of treatment and control groups of mice. From the result it was concluded that andaliman leaves extract did effect to ovary morphology of female mice, because can be effected oogenesis and ovulation. But the result showed that fluctuatif, because maybe the andaliman extract is crude extract that didnâ€™t Â isolation chemistry consist.

PENGUKURAN KADAR PIRIDOKSIN (VITAMIN B6) DALAM DARAH PADA ANAK PENDERITA DEFISIT PERHATIAN DAN GANGGUAN HIPERAKTIVITAS (ADHD) Melvariani Syari Batubara; Yahwardiah Siregar; Herla Rusmarilin; Sri Soviani
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 2, No 1 (2018): Klorofil: Jurnal Ilmu Biologi dan Terapan
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v2i1.1801

This research aimed to study Piridoksin (Vitamin B6) status in blood sample of Attention Deficit Hyperactivity Disorder (ADHD) children. Deductive research using the descriptive method through 2 stage, namely : (1) Taken of blood sample, and (2) Analysis of Piridoksin (Vitamin B6) status in blood sample was performed by using High Performance Liquid Chromatograpy (HPLC) method. Data collected and assessed by once in one time. Data were analyzed using descriptive analysis techniques and Chi Square tests (X2) by Computer programs (SPSS 15). The result of the assessment of Piridoksin (Vitamin B6) status in blood sample of ADHD children that had been was considered statistically highly significant a reduce of the assessment of Piridoksin (Vitamin B6) status in blood sample of ADHD children by 0,422 ng/ml compared with laboratory reference (control) by 0,36-1,8 ng/ml, HA<C** (p<0,001). This was considered a reduction in Piridoksin (Vitamin B6)) concentrations induces of the Hyperactivity children by 95%.

Biotransformasi Tartrazin oleh Bakteri Usus Manusia Melvariani Syari Batubara; Nurmaini Ginting
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 4, No 2 (2018): Februari
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v4i2.972

The aimed of this research was to investigated human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri), to investigated dose of tartrazine, and to investigated biotransformation of tartrazine by human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri). The method of this research was experiment with significant correlation to investigated biotransformation of tartrazine by human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri). This study is primarily focussed on made bacteria medium, made standart inoculums of bacteria, and evaluation for activity of human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri). The results obtained were attributed to the human intestinal bacteria was used in this research was biotransformated tartrazine. From the results that had been increase dose of tartrazine correlation with increase biotransformation by human intestinal bacteria. Highest dose of tartrazine was 15 mg, and highest biotransformation by Vibrio fischeri, which rate of zone decolourisation diameter was 1,22 cm, and rate of zone decolourisation was 1,44 cm2. Statistically showed significantly in correlation dose of tartrazine with rate of zone decolourisation (0,001 < P < 0,05), but statistically showed non significantly in correlation bacteria with rate of zone decolourisation (0,05 < P < 0,268).

HUBUNGAN KADAR TARTRAZIN DAN SENG (ZN) DALAM DARAH PADA ANAK PENDERITA DEFISIT PERHATIAN DAN GANGGUAN HIPERAKTIVITAS (ADHD) Melvariani Syari Batubara; Yahwardiah Siregar; Herla Rusmarilin; Sri Soviani; Husnarika Febriani
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 4, No 1 (2017): Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v4i1.960

This research aims to study Tartrazine and Zinc (Zn) status in blood sample of Attention Deficit Hyperactivity Disorder (ADHD) children. Deductive research using the descriptive method through 3 stage, namely : 1. Taken of blood sample, 2. Analysis of Tartrazine status in blood sample was performed by using High Performance Liquid Chromatograpy (HPLC) method, and 3. Analysis of Zinc (Zn) status in blood sample were measurements by using Atomic Adsorbance Spectrophotometry (AAS) method. Data were analyzed using descriptive analysis techniques and Chi Square tests (X2) by Computer programs (SPSS 15). The result of the assessment of Tartrazine status in blood sample of ADHD children that had been was considered statistically highly significant a increase of the assessment of Tartrazine status in blood sample of ADHD children by 130 mg/ml compared with laboratory reference (control) by 28 mg/ml, HA>C** (p<0,001). The result of the assessment of Zinc (Zn) status in blood sample of ADHD children that had been was showed statistically highly significant a reduce of the assessment of Zinc (Zn) status in blood sample of ADHD children by 0,37 µg/ml compared with laboratory reference (control) by 0,64-1,18 µg/ml, HA<C** (p<0,001). This was considered a reduction and increase in Tartrazine and Zinc (Zn) concentrations induces of the Hyperactivity children by 95%.

PENGARUH PEMBERIAN EKSTRAK DAUN ANDALIMAN (Zanthoxylum acanthopodium DC.) TERHADAP HISTOLOGIS OVARIUM MENCIT (Mus musculus L.) Melvariani Syari Batubara; Emita Sabri; Masitta Tanjung
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 6, No 2 (2020): Februari
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v6i2.2409

Research the effect of andaliman leaf extract on mice ovaries, volume test material using oral gavage needles as much 0.1 ml / 10 g bb 4 days before and after estrus stage and using RAL non-factorial method consisting of control and treatment group. Treatment consisted of factor test material with dose 2500, 5000, 10000, 20000, 40000 ppm. Chemical content of andaliman leaf extract shown from results study are alkaloids, steroids and saponins. Giving treatment of andaliman leaf extract for 9 days on histological data in the number primary, secondary, De Graff, and atretic follicles experienced fluctuating changes, but statistically did not show a significant difference between treatment and control mice groups, while different results were significantly shown in the number of corpus luteum. Parameters diameter of primary follicles, De Graff, and corpus luteum tended to experience changes that fluctuated, but statistically did not show the difference between the treatment and control mice groups, while the diameter of secondary and atretic follicles showed significantly different results. From the results study, administration of andaliman leaf extract affects histological mice ovaries, because it affects oogenesis and ovulation, but fluctuates, possibly due to crude extract, which has not isolated chemical content.

Hasil Validasi Soal Tes Hasil Belajar Mahasiswa Menggunakan Video Pembelajaran Kultur Jaringan Tanaman Berbasis Masalah Melvariani Syari Batubara; Ayunda Sabrina Sormin
Jurnal Pendidikan Biologi Vol 7, No 3 (2018): Jurnal Pendidikan Biologi
Publisher : Magister Pendidikan Biologi, Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpb.v7i3.10605

The purpose of this study was to determine the results of the essay form validation test for student learning outcomes tests using problem-based plant tissue culture learning videos. The method used in this study is included in the development research to develop test questions on student learning outcomes by using problem-based plant tissue culture learning video products. The stages of the activities carried out are: (1) Research and Information colletion (research and data collection), (2) Planning (planning), (3) Develop Preliminary form of Product (development of the initial product draft), (4) Preliminary Field Testing ( initial field trials), (5) Main Product Revision (revised trial results), (6) Main Field Testing (main product field test), and (7) Operational Product Revision (product revision). From this study, the results show that where valid questions in cycle I are 10 questions, while in cycle II there are 11 valid questions. The first and second cycle test reliability is very high. Based on the calculation results of the above test differentiation in cycle I there are 7 excellent questions, 1 good question, 2 enough questions, while in cycle II there are 6 very good questions, 3 questions which are good, 1 problem is enough, and 1 problem is bad. Based on the index of difficulty in cycle I there are 10 questions that are easy, while in cycle II there are 10 questions that are easy and there is one problem in the medium category.

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