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INDONESIA
Jurnal Biodjati
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Articles 181 Documents
Effect of Nitrogen Source on Growth Endophytic Yeast from Salacca edulis Reinw. and Bread Quality Analysis Nuzulul Zahroh; Ulfah Utami; Nur Kusmiyati
Jurnal Biodjati Vol 7, No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.16854

Abstract

east biomass is often used in the fermentation of bread dough. Dough fermentation can be maximized by adding a nitrogen source. This study used yeast isolates from salak pondoh (YIS-3, YIS-4, and YIS-7). The purpose of this study was to determine the effect of nitrogen addition on yeast growth and bread quality. This study used an experimental approach. The results of the growth study showed that all isolates treated with 0.05% urea produced higher biomass and cell counts than those treated with control. The highest biomass was produced by YIS-7, which was 3.81 g/300mL, while the highest number of cells was produced by YIS-3, which was 29.02x106 cells/mL. The percentage of proofing results showed that all yeast isolates treated with 0.05% urea needed a longer time to achieve the highest proofing. However, the volume of bread after baking showed better results than those treated with control. The largest volume of bread produced by YIS-3, was 972.14 cm3. The results of the organoleptic test showed that P<5%, which means that all treatments had a significant effect on the taste, aroma, color, and texture of the bread. Overall, the panelists gave good acceptance of the bread fermented by YIS-3 with 0.05% urea treatment. So it can be concluded that the addition of urea with a concentration of 0.05% in YIS-3 gave the best effect on the yeast growth and bread quality.
Diversity of the Cave-Dwelling Bat (Chiroptera) in the Ngobaran Coastal Area, Karst of Gunung Sewu Tatag Bagus Putra Prakarsa; Rizka Apriani Putri; Yunita Fera Rahmawati; Abdullah Dolah Dalee
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.20163

Abstract

Bats (Chiroptera) are divided into two suborders (Yinpterochiroptera and Yangochiroptera). More than 50% of species of bats use caves as their roosting sites. Thus, they play a crucial role in the cave ecosystem. For that reason, they also exist as keystone species in the karst area. Gunung Sewu is one of the karst areas in Indonesia that best exemplifies tropical karst. Furthermore, Gunung Sewu is still at risk of habitat loss despite being designated as a Geopark. This study aimed to understand the diversity of bats that live in caves in the karst region of Gunung Sewu, specifically in four caves near Ngobaran Beach between April and June 2020. A harp net and misnet placed at the cave’s entrance were used to capture bats for data collection. After they were captured, the bats were identified using Morphometry and the Shannon-Wiener index. Through another index, Margalef index, the bat diversity in the four cave habitats was expressed, with a discovery that there are many different species. Based on the Jaccard similarity index, bats were categorized again using cluster analysis and the unweighted pair-group method using arithmetic averages (UPGMA). A total of nine species across five families were identified. The diversity of existing species variety was also recognized by analyzing the composition of the four cavern inhabitants. The four cave ecosystems’ bat diversity was divided into three categories: moderate variety, low similarity, and high species diversity. Except for Cekelan 1 Cave and Gebyog Cave (P=0.015), other variations did not demonstrate a meaningful difference (P0.05). This demonstrates how different each ecosystem is. Therefore, they could be classified as potentially spoiled habitats, demanding additional conservation efforts.
Soil Contamination in Randukuning Landfill: Morphological-Physiological Responses of Celosia argentea L. and Cleome rutidosperma D.C. Dwi Puji Hayati; Andhika Puspito Nugroho
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.18524

Abstract

The soil in the Landfill area has generally been contaminated by various types of pollutants, including heavy metals, microplastics, ammonia, chloride, benzene, toluene, ethylene, ethylene benzene, and xylene (BTEX). At high concentrations, the pollutant can cause toxic effects on plants. The study aimed to evaluate the morphological and physiological conditions of C. argentea L. and C. rutidosperma D.C. after being planted on Randukuning landfill soil. Plant species were planted on landfill soil in the greenhouse for two months. Morphological observations (stem length, root length, and the number of leaves) and physiological (biomass of roots, stems, and leaves) were carried out on days 0, 3, 7, 14, 21, and 28 after planting on landfill soil. The results showed that C. argentea L. and C. rutidosperma D.C. did not show morphological and physiological effects. Both species can grow well on landfill soils. Therefore, these species can be potential phytoremediation agents.
Orchid (Orchidaceae) Inventory in Isau-Isau Wildlife Conservation Resort Area IX Lawang Agung Village, South Sumatra Felia Melinda. H; Harmida Harmida; Nita Aminasih
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.19271

Abstract

Orchidaceae is one of the plant families which occupies 7-10% of the total flowering plants in the world. Of the 25,000 species of orchids in the world, 6,000 species are found in various forests in Indonesia, including Sumatra. South Sumatra save high diversity of orchid and one of them is in Isau-Isau Wildlife Conservation. This area has various types of plants, including orchids. Diversity orchids in the area have not gotten any attention, so the data regarding orchids in the Isau-Isau Wildlife Conservation, especially in Resort Conservation Area IX Lawang Agung Village, Mulak Ulu District, Lahat Regency, South Sumatra still limited and not well documented, so that need further research to know types of orchids in the area. The research found 22 species, consisting of 21 epiphyte orchids and 1 terrestrial orchid. Many orchids were found in the secondary forest of 16 species. An endemic orchid of South Sumatra was found in this research, it is Vanda foetida. Host plants found were 11 plants, 7 can identify and 4 not can be identified.
Pharmacophore Analysis of Monoterpene Melaleuca leucadendra as an Inhibitor for 3CLPro of the SARS-CoV-2 Ismi Farah Syarifah; Yani Suryani; Ghea Gema Adzani; Isma Dwi Kurniawan; Opik Taupiqurrohman
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.20496

Abstract

The monoterpene compound has been reported to have antiviral activities . This study aimed to test the monoterpene compound in Melaleuca leucadendra to inhibit the SARS-CoV-2 virus. The monoterpenes tested were α-Pinene, β-Pinene, Linalool, α-Terpineol, and Terpinene-4-Ol. The method used was computational through pharmacophore analysis. The indicator for the quality of the compound was the fit score. A fit score of more than 50% indicates a good-quality compound, while a fit score of less than that indicates a  poor-quality compound. Based on the analysis results, the monoterpene compound in Melaleuca leucadendra can potentially inhibit the SARS-CoV-2 virus directly through the inhibition of 3C-like protease. The linalool showed a fit score of 55% with interactions of hydrophobic, electrostatic, and hydrogen bonds. All the compounds did not inhibit the metabolic process and were safe, possibly having no side effects based on ADMET analysis.
Hemotoxicity of Hairy Fig (Ficus hispida L.f.) Fruits on Male Wistar Rats (Rattus norvegicus Berkenhout, 1769) Laksmindra Fitria; Annisa Lintang Sari; Lisa Handayani; Slamet Widiyanto
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.19241

Abstract

Hairy fig (Ficus hispida) fruits (HFF) are widely consumed as food and traditional medicine in several West Asian countries, both the unripe fruit (UHFF) and ripe fruit (RHFF). However, they are not commonly utilized in Indonesia. Acute oral toxicity studies reported No Observed adverse effect level (NOAEL). Further, a reproductive toxicity study found that UHFF boosted spermatogenesis and increased the quality and quantity of spermatozoa. Meanwhile, RHFF exhibited the opposite effects. To provide comprehensive information from the previous study, this research was conducted to evaluate the hemotoxicity of UHFF and RHFF about their impact on the male reproductive system. Nine Wistar rats were assigned into three groups: the first group received UHFF juice, the second group received RHFF juice, and the third group received distilled water as control. The volume of each treatment was 2 mL/individual/day for 77 days. On days 0, 28, and 77, blood samples were collected for routine hematological profile examination using a hematology analyzer (Sysmex®XP-100). Data were analyzed using a one-way ANOVA test and Duncan's test (α=0.05) to discover significant differences between groups and times. Results showed that consuming hairy fig fruit, especially the UHFF, had an unfavorable effect on erythrocytes resulting in hypochromic microcytic anemia. Still, there was no adverse effect on leukocytes and platelets. Anemia may have occurred due to the presence of hemotoxic compounds that interfere with the synthesis and binding of hemoglobin or because the hairy fig fruit filtrates were oxidized, thereby increasing the level of oxidative stress within the body, of which is a drop in hemoglobin levels.
Bamboo Diversity in the Maluku Islands, Indonesia Yayah Robiah; Dewi Rosalina; I Putu Gede P. Damayanto
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.18713

Abstract

There is little documentation of bamboo species diversity and distribution in the Maluku Islands of Indonesia. This study aimed to provide information on the diversity and distribution of Maluku’s bamboo species. The research was conducted in March‒April 2021 at the Herbarium Bogoriense (BO), National Research and Innovation Agency. Herbarium specimens of bamboo in BO collected from the Maluku Islands (Provinces of Maluku and North Maluku) were studied alongside online scanned images of herbarium specimens. Bamboo species were re-identified and validated. A literature study was conducted to enrich the data. A distribution map of bamboo species in the Maluku Islands was compiled. Data were analyzed and presented descriptively. We built an identification key for the bamboo species in the Maluku Islands. There are 16 species and eight genera of bamboo in the Maluku Islands: Bambusa glaucophylla, B. maculata, B. multiplex, B. spinosa, B. tuldoides, B. vulgaris, Dendrocalamus asper, Gigantochloa atter, G. robusta, Neololeba amahussana, N. atra, Phyllostachys aurea, Racemobambos ceramica, Schizostachyum brachycladum, S. lima, and Thyrsostachys siamensis. These bamboo species were found across Maluku Province (Buru, Ambon, Seram, Kei Kecil, Kei Besar, Yamdena, and Tanimbar) and North Maluku Province (Ternate, Tidore, Halmahera, Obi, and Sula) at an altitude of 1‒1100 m above sea level in primary and secondary forests, limestone, peatland edges, city parks, and gardens.
An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate Kahar Muzakhar; Farah Salma Elida; Ramdhan Putrasetya; Siswoyo Siswoyo; Rudju Winarsa; Hidayat Teguh Wiyono
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.20279

Abstract

An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.
Diversity of Amphibians (Order Anura) in Cibodas Resort Tourism Route, Gunung Gede Pangrango National Park Teti Rostikawati; Dimas Prasaja; Ferra Handayani
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.19238

Abstract

Cibodas Resort is one of the protected areas in Gunung Gede Pangrango National Park which is capable of supporting amphibian life. Amphibians can be used as bioindicators of environmental quality, especially aquatic ones. Amphibian studies are found to be interesting, but publications on amphibian data, especially at Resort Cibodas, are still rare. The publications regarding amphibian data of the Anura order, especially in the Cibodas Resort, have not been found. This Study aimed to determine the diversity of amphibians of the Order Anura in the Cibodas Resort tourist route. This research was an exploratory research conducted from September to October 2021. The research method used the Visual Encounter Survey method combined with the Line Transect method by following a predetermined path. The data was retrieved through the form of primary data (number of species of the Order of Anura in the Cibodas Resort tourist route). Based on the results of research at 3 sites, 12 species from 6 families were found with 192 individuals. The diversity index is classified as moderate with a value of 2.15, the evenness index is classified as high with a value of 0.86, and the dominance index is classified as low with a value of 0.14. The species that dominate in the 3 observation sites are from the family Ranidae namely Odorrana hosii and Huia masonii, the family Rhacophoridae namely Rhacophorus margaritifer and the family Dicroglossidae namely Limnonectes kuhlii and L. microdiscus.
Effects of Passiflora foetida on the Number of Purkinje Cells in the Cerebellum of Albino Rats Rina Priastini Susilowati; Budiman Hartono
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.14298

Abstract

 Permot (Passiflora foetida) is one type of plant that has been used as a mosquito coil. Besides being effective at killing Aedes aegypti mosquitoes, mosquito coils made from permot leaf extract are also effective at killing German cockroaches and house flies. Until now there have been no reports stating the side effects of using permot leaf extract in humans. This study was designed to demonstrate the harmful side effects of inhalation of smoked mosquito coils made from permot leaf extract on the cerebellar cortex of adult male rats. Thirty-five adult male rats were used in this study. Rats were divided into 7 groups, namely group 1 as a negative control (without exposure), group 2 as a positive control (transfluthrin exposure 3000 ppm), groups 3 to 7 were treatment groups of permot leaf extract with graded doses (500 ppm, 1000 ppm, 2000 ppm, 3000 ppm, and 4000 ppm), and was treated for 12 weeks. All rats were sacrificed in a timely manner and cerebellar specimens were taken, prepared and observed using a light microscope. Previously, rats were weighed periodically to determine the effect of toxicity. The results showed that exposure to mosquito coil smoke made from 3000 ppm Transflutrin and 4000 ppm permot leaf extract caused weight loss in rats. Likewise on the weight of the rat cerebellum. The effect of decreasing the number of Purkinje cells also occurred in the group exposed to mosquito coils made from Transflutrin 3000 ppm and permot leaf extract 4000 ppm. Therefore, the safe and effective dose of mosquito coils made from permot leaves is less than 4000 ppm, because exposure to doses of more than 4000 ppm can cause degenerative changes in the cerebellar cortex.