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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
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Articles 20 Documents
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Search results for , issue "Vol 7, No 2 (2015): September 2015" : 20 Documents clear
Aplikasi Pupuk Kandang Kotoran Ayam pada Tanaman Kacang Tanah (Arachis Hypogeae L.) Marlina, Neni; Aminah, Raden Iin Siti; -, Rosmiah; Setel, Lusdi Ramlan
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3957

Abstract

Pupuk kandang kotoran ayam diharapkan dapat memperbaiki sifat fisik, kimia dan biologi tanah, sehingga dapat menyuburkan tanah dan membantu dalam menyumbangkan unsur hara yang dapat digunakan dalam meningkatkan hasil kacang tanah. Penelitian ini bertujuan untuk mendapatkan takaran pupuk kandang kotoran ayam yang tepat dalam meningkatkan produksi tanaman kacang tanah. Penelitian ini telah dilaksanakan di kebun petani di Desa Payakabung Kecamatan Indralaya Utara Kabupaten Ogan Ilir dari bulan Januari sampai dengan April 2014. Rancangan yang digunakan pada penelitian ini adalah Rancangan Acak Kelompok (RAK) dengan tiga perlakuan dan delapan kelompok, sehingga berjumlah 24 petak penelitian dan setiap petak diambil 10 tanaman sebagai sampel . Perlakuannya adalah takaran pupuk kandang kotoran ayam 5, 10 dan 15 ton ha-1. Hasil penelitian menunjukkan bahwa takaran pupuk kandang kotoran ayam sebanyak 10 ton ha-1 memberikan pertumbuhan dan produksi terbaik dengan ditunjukkan produksi per petak sebesar 2,73 kg petak-1.Poultry manure is expected to improve soil physical, chemical and biological properties. It can improve soil fertility and help in nutrients contribution that can be used to increase the yield of peanut. This study aimed to get the right dose of poultry manure fertilizer in increasing the production of ground peanut plants. This research was conducted in farmyard in the North Indralaya Payakabung District of Ogan Ilir from January to April 2014. The design used in this study was a randomized block design with three treatments and eight groups, thus consisting 24 research plots and each plot was taken as a sample of 10 plants. The treatments of poultry manure fertilizer rate 5, 10 and 15 ton ha-1. The results showed that poultry manure fertilizer rate as much as 10 tons ha-1 gave the best growth and production of 2.73 kg per plot.
Potensi Ekowisata di Kawasan Mangrove, Desa Mororejo, Kabupaten Kendal Fahrian, Haikal Hilman; Putro, Sapto P.; Muhammad, Fuad
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3953

Abstract

Desa Mororejo memiliki kawasan mangrove yang belum dimanfaatkan secara optimal. Salah satu upaya yang dapat dilakukan untuk mengoptimalisasi adalah dengan menjadikan kawasan ekowisata. Penelitian dilaksanakan dari bulan Agustus-November 2014 yang dilakukan melalui survei yang dilanjutkan dengan kegiatan pengamatan kondisi fisik kimia dan biologi kawasan, inventarisasi keanekaragaman fauna, analisis vegetasi, serta pengambilan data persepsi masyarakat lokal. Analisis data menggunakan analisis kesesuaian wisata mangrove dan analisis SWOT (StrengthsWeaknessOpportunityTreats). Berdasarkan hasil penelitian, kawasan mangrove Desa Mororejo didominasi oleh tiga jenis mangrove yaitu Rhizophora mucronata, Rhizophora stylosa, dan Avicennia marina. Fauna yang ditemukan antara lain burung, ikan, dan crustacea. Indeks kesesuaian untuk kegiatan wisata mangrove termasuk kategori sesuai bersyarat (63.24%). Strategi alternatif pengelolaan ekowisata mangrove yang diprioritaskan meliputi: melibatkan masyarakat lokal dalam kegiatan ekowisata (skor 2,834); meningkatkan peran serta Dinas terkait (skor 2.517); dan adanya zonasi wilayah supaya tidak terjadi gesekan dengan berbagai pihak (skor 2.25).Mangrove areas at Mororejo village have not been optimally utilized. One effort to accelerate the optimalization of the area is by empowering its ecotourism. The study was conducted in August-November 2014 using a survey method, inventory of assorted fauna, vegetation analysis, and data collection of local community perception. Data analysis was conducted by feasibility analysis and SWOT (StrengthsWeaknessOpportunityTreats) analysis to determine the alternatives strategy in exploring the potency of eco-tourism. Based on the result of this study, mangrove areas in Mororejo were dominated by three types of mangrove, i.e. Rhizopora mucronata, Rhizopora stylosa, and Avicennia marina. Fauna found in the areas were birds, fish, and crustacean. Feasibility index for mangrove tourism at mangrove areas at Mororejo village fell to category of conditionally feasible (63.24%). The alternative strategy in managing mangrove ecotourism at Mororejo village should involve the local community in handling ecotourism activities such as fishing activitity, birdwatching, and mangrove conservation (score 2.83); and also empower the related department (score 2.52), and zonation based areas should be implemented, therefore horizontal conflict could be avoided (score 2.25).
The Effect of Annona Muricata Leaves Towards Blood Levels of Cxcl9 and Lymphoblast (Study in Cerebral Malaria Phase of Swiss Mice) Gadalla, Mohamed M.Y.; Dharmana, Edi; Jamiatun, Kiss; Laksono, Budi
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.4078

Abstract

Cerebral malaria (CM) forms part of the spectrum of severe malaria, with a case fatality rate ranging from 15% in adults in southeast Asia to 8.5% in children in Africa. A.Muricata was used to cure Malaria in traditional medicine. The research will examine the effect of it in the chemokine (C-X-C motif) receptor 3 (CXCR3) binding chemokines, including chemokine (C-X-C motif) ligand 4 (CXCL4), CXCL9. The intervented mice group were infected then the its spleen were cultured , incubation 72 hours and then analyzed the result. The CXCL9 level of PbA-infected mice treated with A. muricata are lower than group of infected mice without treatment. Lymphoblast level of PbA-infected mice treated with A. Muricata are higher than group of infected mice without treatment. A. Muricata treatment cure in the CM in the mice and may be a potential treatment in human CM.Cerebral malaria (CM) adalah keadaan infeksi malaria yang berat dengan tingkat kefatalan dari 15% di Asia tenggara dan 8% di Afrika. A. Muricata secara tradisional dipakai mengobati CM. Riset ini meneliti pengaruh A. Muricata pada ikatan chemokine (C-X-C motif) reseptor 3 (CXCR3)termasuk chemokine (C-X-C motif) ligand 4 (CXCL4) dan CXCL9. Kelompok mice intervensi diinfeksi dan limfanya di culture dalam inkubator 72 jam untuk dianalisis. Kadar PbA CXCL9 pada mencit intervensi yang diberi A. Muricata lebih rendah dari pada kontrol. Kadar PbA limfoblast intervensi lebihtinggi dari pada kontrol. A. Muricata memperbaiki CM pada mencit dan berpotensi sebagai pengobat pada CM manusia.
Preliminary Study on the Single Nucleotide Polymorphism (SNP) of XRCC1 Gene Identificationto Improve the Outcomes of Radiotherapy for Cervical Cancer Tetriana, Devita; Mailana, Wiwin; Kurnia, Iin; Syaifudin, Mukh
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3949

Abstract

Cervical cancer is the most fatal disease among Indonesian women. In recognition of the substantial variation in the intrinsic response of individuals to radiation, an effort had been done to identify the genetic markers, primarily Single Nucleotide polymorphisms (SNPs), which are associated with responsiveness of cancer cells to radiation therapy. One of these SNPs is X-ray repair cross-complementing protein 1 (XRCC1) that is one of the most important genes in deoxyribonucleic acid (DNA) repair pathways. Meta-analysis in the determination of the association of XRCC1 polymorphisms with cervical cancer revealed the potential role of XRCC1 polymorphisms in predicting cell response to radiotherapy.Our preliminary study with real-time polymerase chain reaction (RT-PCR) showed that radiotherapy affected the XRCC1 gene analyzed in blood of cervical cancer patient. Other published study found three SNPs of XRCC1 (Arg194Trp, Arg280His, and Arg399Gln) that cause amino acid substitutions. Arg194Trp is only SNPs that associated with high risk of cervical cancer but not others. Additionally, structure and function of this protein can be altered by functional SNPs, which may lead to the susceptibility of individuals to cancers. Anotherstudy found G399A polymorphisms. We concluded that SNP of this DNA repair genes have been found to be good predictors of efficacy of radiotherapy.Kanker serviks adalah penyakit yang paling fatal pada perempuan di Indonesia. Untuk memahami variasi substansial respon intrinsik individual terhadap radiasi, suatu usaha telah dilakukan untuk mengidentifikasi petanda genetik, terutama Single Nucleotide polymorphism (SNP), yang berkaitan dengan responsel kanker terhadap terapi radiasi. Satu dari SNP tersebut adalah X-ray repair cross-complementing protein 1 (XRCC1) yang merupakan satu dari gen paling penting dalam lajur perbaikan asam deoksiribonukleat (DNA). Meta-analysis dalam penentuan hubungan polimorfisme XRCC1 dengan kanker serviks menemukan adanya peranan potensial polimorfisme XRCC1 dalam memprediksi respon sel terhadap radioterapi. Studi awal kami menggunakan real-time polymerase chain reaction (RT-PCR) menunjukkan bahwa radioterapi mempengaruhi gen XRCC1yang dianalisis dalam darah pasien kanker serviks. Studi yang telah dipublikasi menemukan tiga SNP dari XRCC1 (Arg194Trp, Arg280His, dan Arg399Gln) yang menyebabkan substitusi asam amino. Arg194Trp merupakan satu-satunya SNP yang berkaitan dengan tingginya risiko kanker serviks, tetapi tidak pada yang lain. Di samping itu, strukturdan fungsi protein ini dapat berubah oleh SNP fungsional, yang mengarah ke kerentanan individu untuk menderita kanker. Studi lain menemukan polimorfisme G399A. Kami menyimpulkan bahwa SNP dari gen perbaikan DNA ini merupakan prediktor yang baik dari keberhasilan radioterapi.
Kajian Efektifitas Rhizoctonia SP Mikoriza Dataran Rendah dan Sedang pada Tingkat Keparahan Penyakit (Dsi) Anggrek Phalaenopsis amabilis terhadap Fusarium sp. Soelistijono, R.
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3954

Abstract

Anggrek Phalaenopsis amabilis atau yang lebih dikenal sebagai anggrek bulan merupakan plasma nutfah dan tersebar diberbagai tempat di Indonesia. Keberadaan anggrek ini semakin berkurang karena serangan jamur patogen. Fusarium sp. merupakan patogen yang paling banyak menyerang anggrek P. amabilis (Chung et al., 2011) dibandingkan dengan jamur patogen lainnya. Serangan Fusarium sp. akan menyebabkan daun bewarna kuning dan membusuk. Pada penelitian ini dilakukan penggunaan Rhizoctonia mikoriza yang diisolasi dari P. amabilisdi wilayah Sleman dan Surakarta menurut metode Bayman et al. (Otero, 2002), untuk mengetahui perbedaan efektifitasnya didalam menghambat pertumbuhan Fusarium sp. yang diisolasi menurut metode Barnet dan Hunter (1972). Penelitian menggunakan metoda RAL dengan 2 perlakuan dan 6 ulangan selama 8 bulan, masing-masing diberi dan tanpa Rhizoctonia mikoriza dan Fusarium sp. Hasil penelitian menunjukkan terjadi peningkatan pertumbuhan vegetatif pada anggrek P. amabilis yang diprainokulasi Rhizoctonia mikoriza yang diisolasi dari P. amabilis di Surakarta (M2) terhadap Fusarium sp. Tingkat keparahan penyakit (DSI) anggrek P. amabilis terhadap Fusarium sp. yang diprainokulasi Rhizoctonia mikoriza dari P. amabilis di Sleman lebih tinggi dibanding dari SurakartaPhalaenopsis amabilis or better known as orchids in the germplasm and scattered in various places in Indonesia. The existence of this orchid wane since the attacks of pathogenic fungi. Fusarium sp. is the most attacking pathogen P. amabilis orchid (Chung et al., 2011) compared to other pathogenic fungi. Fusarium sp. attack will cause the leaves to rot and yellow colored. In this study the use of mycorrhizal Rhizoctonia isolated P. amabilis from Sleman and Surakarta in the green house UTP to determine differences in effectiveness in inhibiting the growth of Fusarium sp. Research using the method of RAL with 6 replications for 8 months.The results showed an increase in the vegetative growth of P. amabilis orchid mycorrhiza which prainoculated with Rhizoctonia isolated from P. amabilis in Surakarta (M2) against Fusarium sp. The severity of disease (DSI) P. amabilis orchid against Fusarium sp. which prainoculated with mycorrhizal Rhizoctonia from P. amabilis in Sleman higher than from Surakarta
Analysis of Proximate and Protein Profile of Kefir from Fermented Goat and Cow Milk Hidayat, Erwin; Kinayungan W, Irna; Irhas, Muhammad; Sidiq, Fathurrahman
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3950

Abstract

This research aims to analyze the characteristics of proximate and protein profile in kefir from fermented goat milk and cow milk with different concentration of kefir grains. The research design was true experimental with Completely Randomized Design (CRD) of 3 repetitions. The research procedures consisted of kefir production, proximate analysis and protein profile characterization. Proximate assay result was analyzed by using LSD, whereas the protein profile was analyzed by descriptive qualitative method. Based on the analysis of kefir proximate levels, the kefir grain (5%) showed the highest proximate level of both kefirs from goat milk and cow milk. The analysis of protein profile of cow milk kefir showed 75 kDa of protein ribbon, while the goat milk kefir showed 48 kDa, 60 kDa and 75 kDa. Therefore it can be concluded that the proximate level of goat and cow milk kefir with different concentration of kefir grains showed significant differences in the nutrition content as well as its protein profiles.Tujuan dari penelitian ini adalah menganalisis karakteristik proksimat dan profil protein pada kefir hasil fermentasi susu kambing dan susu sapi dengan konsentrasi biji kefir yang berbeda-beda. Penelitian ini adalah eksperimen murni, dengan Rancangan Acak Lengkap (RAL) 3 kali ulangan. Prosedur penelitian meliputi pembuatan kefir, analisis proksimat dan profil protein. Data hasil proksimat dianalisi uji BNT, sedangkan profil protein dianalisis deskriptif kualitatif. Berdasarkan analisis kadar proksimat kefir, kefir grains 5% menunjukan kadar proksimat paling tinggi baik pada kefir susu kambing dan susu sapi. Sedangkan analisis profil protein kefir susu sapi menunjukan pita protein 75 kDa, pada kefir susu kambing yaitu 48 kDa, 60 kDa dan 75 kDa. Simpulan dari penelitian ini bahwa kadar proksimat kefir susu kambing dan susu sapi dengan konsentrasi kefir grains yang berbeda menunjukan perbedaan kandungan yang berbeda secara signifikan dengan konsentrasi kefir grains yang paling optimal yaitu 5%. Sedangkan profil protein kefir susu sapi ditemukan pita protein yaitu 75 kDa, dan kefir susu kambing yaitu 48 kDa, 60 kDa dan 75 kDa.
Potensi Sumberdaya Alam Hayati Kawasan Mangrove Pasar Banggi Kabupaten Rembang Sebagai Objek Ekowisata -, Kusaeri; Putro, Sapto P.; Wasiq, Jafron
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3955

Abstract

Kawasan Mangrove Pasar Banggi merupakan salah satu tegakan mangrove yang masih baik dan sudah direboisasi sejak tahun 1960-an. Kawasan tersebut memiliki sentra persemaian mangrove yang cukup dikenal oleh masyarakat luas di Pulau Jawa. Penelitian ini bertujuan untuk mengetahui kondisi vegetasi dan kualitas lingkungan mangrove serta menyusun rekomendasi strategi pengembangan ekowisata mangrove di kawasan tersebut. Metode penelitian menggunakan deskriptif analitik melalui pendekatan kuantitatif. Hasil penelitian menunjukkan bahwa hutan mangrove Pasar Banggi seluas 14,88 ha dalam kondisi cukup baik dengan identifikasi jenis mangrove yang ditemukan Avicennia marina, Rhizophora apiculata, Rhizophora mucranata, Rhizophora stylosa, Sonneratia alba, dan Sonneratia caseolaris. Indeks Nilai Penting (INP) untuk vegetasi mangrove tertinggi terdapat pada jenis Rhizophora mucronata sebesar 281,82%, dan Indeks Kesesuaian Wisata Mangrove pada 9 lokasi (plot) diperoleh data 61,54% yang masuk kedalam kategori sesuai bersyarat. Strategi pengembangan potensi mangrove berdasarkan Matriks Grand Strategy SWOT berada pada kuadran I yang berarti menggunakan strategi agresif. Dalam strategi ini yang diprioritaskan adalah penentuan master plan zonasi pemanfaatan secara terpadu, manajemen pengelolaan objek ekowisata yang profesional serta membangun komitmen semua pihak dalam menjaga kelestarian lingkungan.Mangrove areas of Pasar Banggi is one of the mangrove stands has been reforested since the 1960s. The region has a mangrove nursery centers well known by the public on Java Island. This study aimed to determine the condition of mangrove vegetation and environmental quality. It also aimed to make a recommendation for mangrove eco-tourism development strategy. The research method used was descriptive qualitative analytical approach. The results showed that mangrove forests of Pasar Banggi was 14.88 ha in good condition with the identification of mangrove species found were Avicennia marina, Rhizophora apiculata, Rhizophora mucranata, Rhizophora stylosa, Sonneratia alba, and Sonneratia caseolaris. The importance value index for the highest mangrove vegetation found in Rhizophora mucronata by 281.82%, and travel suitability index of mangrove at 9 locations (plots) acquired 61.54% of data that goes into the category of conditionally appropriate. Mangrove potency development strategy was based on SWOT Matrix Grand Strategy was in quadrant I, which means using aggressive strategy. The strategy priority was the determination of the zoning master plan for integrated data collection, the professional management of ecotourism object and to raise the commitment of all parties to keeping environment sustainability.
Proteases from Latex of Euphorbia spp. and Its Application on Milk Clot Formation Fibriana, Fidia; Upaichit, Apichat
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3951

Abstract

Crude proteases were extracted from Euphorbiaceae family, i.e. E. milii var imperata, E. trigona, and E. maculata. Among those three crude proteases, the activity of protease from E. trigona was the highest (812.50 U/ml), whereas E. milii and E. maculata crude proteases activity were 298.60 U/ml and 95.80 U/ml, respectively. E. maculata protein concentration was the highest among those three crude enzymes (1.206 mg/ml). The optimum pH and temperature of the enzymes were pH 7.0, pH 6.0, pH 6.5 and 60 C, 50 C, and 50 C, respectively. Crude protease from E. milii var imperata, E. trigona, and E. maculata retained proteolytic activity over a wide range of pH (5.09.0) and temperature (up to 65 C) with casein as substrate. All crude proteases showed milk clotting activity ranged from 0.58 U/ml to 1.01 U/ml. Thus, these crude proteases are potential to be applied in dairy industries. However, further study on enzyme purification and characterization are necessary to obtain high purity of proteases before its application.Protease kasar berhasil diekstrak dari tanaman family Euphorbiaceae, yaitu E. milii var imperata, E. trigona, dan E. maculata. Diantara ketiga protease tersebut, aktivitas protease tertinggi diperoleh dari E. trigona (812,50 U/ml), sedangkan aktivitas protease dari E. milii dan E. maculata adalah 298,60 U/ml dan 95,80 U/ml, berturut-turut. Konsentrasi total protein tertinggi terdapat pada protease kasar E. maculata (1,206 mg/ml). pH dan suhu optimum ketiga enzim tersebut adalah pH 7.0, pH 6.0, pH 6.5 dan suhu 60 C, 50 C, and 50 C, berturut-turut. Protease kasar dari E. milii var imperata, E. trigona, dan E. maculata menunjukkan aktivitas proteolitik pada rentang pH 5.09.0 dan rentang suhu sampai 65 C menggunakan kasein sebagai substrat. Semua protease kasar menunjukkan aktivitas penggumpalan susu dengan rentang dari 0,58 U/ml sampai 1,01 U/ml. Berdasarkan hasil yang diperoleh, protease kasar dari ketiga jenis tanaman ini berpotensi untuk diaplikasikan dalam industri olahan susu. Meskipun demikian, studi lanjut mengenai purifikasi dan karakterisasi sangat diperlukan untuk memperoleh protease murni sebelum aplikasi dalam industri makanan, khususnya pada industri olahan susu.
Aktivitas Antibakteri Ekstrak Kerang Pisau (Solen sp.) dan Kerang Simping (Placuna placenta) Rochmawati, Ika; Ibrahim, Muslimin; Ambarwati, Reni
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3956

Abstract

Penelitian ini bertujuan menguji aktivitas antibakteri ekstrak kerang pisau dan kerang simping dalam menghambat pertumbuhan bakteri uji berdasarkan zona jernih yang terbentuk dan menentukan jenis ekstrak kerang yang paling optimal dalam menghambat pertumbuhan bakteri uji. Kerang pisau yang diperoleh dari Pantai Talang Siring Madura dan kerang simping yang diperoleh dari Lamongan diekstrak dengan menggunakan metode ekstraksi bertingkat dengan tiga pelarut,. Pengujian aktivitas antibakteri dilakukan dengan menggunakan rancangan acak lengkap dengan delapan perlakuan dan tiga ulangan, yaitu pemberian kontrol negatif (akuades), kontrol positif (kloramfenikol), ekstrak heksana, ekstrak etil asetat, ekstrak metanol kerang pisau serta ekstrak heksana, ekstrak etil asetat, ekstrak metanol kerang simping. Data yang diperoleh berupa diameter zona jernih, dianalisis dengan analisis varian satu arah dan dilanjutkan dengan uji Duncan. Berdasarkan hasil uji diketahui bahwa ekstrak etil asetat dan metanol kerang pisau serta ekstrak heksana, etil asetat, dan metanol kerang simping menunjukkan aktivitas antibakteri dan mampu menghambat pertumbuhan bakteri uji Staphylococcus aureus FNCC 0047 dan Escherichia coli FNCC 0091. Ekstrak etil asetat kerang pisau terbukti menunjukkan aktivitas antibakteri paling baik, yaitu menunjukkan diameter zona jernih paling besar dibanding dengan ekstrak-ekstrak yang lain. Ekstrak etil asetat kerang pisau mampu menghambat pertumbuhan bakteri Staphylococcus aureus dengan membentuk zona jernih sebesar 32,10 0,17 mm, sedangkan pada bakteri Escherichia coli ekstrak etil asetat kerang pisau mampu menghambat pertumbuhannya dengan zona jernih yang terbentuk sebesar 32,06 1,07 mm.This research aimed to test the antibacterial activities of the extracts of razor clams and window-pane oyster and determine the extract that can inhibit the growth of tested bacteria optimally based on clear zones formed. Razor clams collected from Talang Siring Beach, Madura and window-pane oyster collected from Lamongan. They were extracted using multilevel extraction method using three organic solvents. Antibacterial activities assay were carried out using completely randomized design with eight treatments. All assays were conducted in triplicate. The treatments were negative control (aquades), positive control (100% of chloramphenicol in aquades), hexane extract, ethyl acetate extract, and methanol extract of each razor clams and window-pane oyster. Diameters of inhibiton zone (clear zone) were analyzed using one way analysis of variance and followed by Duncans test. The results revealed that ethyl acetate and methanol extracts of razor clams as well as hexane, ethyl acetate, and methanol extracts of window-pane oyster showed antibacterial activities. These mean they can inhibit the growth of Staphylococcus aureus FNCC 0047 dan Escherichia coli FNCC 0091. Ethyl acetate extract of razor clams showed the highest antibacterial activity, the maximum zone of inhibition (32.10 0.17 mm) was observed against Staphylococcus aureus and the maximum zone of inhibition (32.06 1.07 mm) was observed against Escherichia coli.
Characteristics of Cells from Five Exotic Bamboos after Drywood Termites Incisitermes minor (Hagen) Attack Subekti, Niken; Yoshimura, Tsuyoshi
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3952

Abstract

This study examined the relationship between and the characteristics of five bamboos exotic in the cell walls. For this purpose, the bamboosexotic from Indonesia were separated into cells after drywood termites Incisitermes minor (Hagen) attack. SEM observations revealed that bamboos cells. Moreover, SEM analysis showed that the cellulose isolated from parenchyma cells. These results suggest that all the cellulose micro?brils in five bamboos different characteristics of cell function after drywood termite attack.Penelitian ini menguji hubungan antara karakteristik dan lima bambu eksotis di dinding sel. Untuk tujuan ini, bamboosexotic dari Indonesia dipisahkan ke dalam sel setelah rayap Drywood Incisitermes minor (Hagen) serangan. Pengamatan SEM menunjukkan bahwa sel-sel bambu. Selain itu, analisis SEM menunjukkan bahwa selulosa yang diisolasi dari sel parenkim. Hasil ini menunjukkan bahwa semua brils fi selulosa mikro di lima bambu karakteristik yang berbeda dari fungsi sel setelah serangan Rayap Kayu Kering.

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