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Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology)
Published by Masyarakat Iktiologi Indonesia
ISSN : 16930339     EISSN : 25798634     DOI : https://doi.org/10.32491
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Aims and Scope Aims: Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) aims to publish original research results on fishes (pisces) in fresh, brackish and sea waters including biology, physiology, and ecology, and their application in the fields of fishing, aquaculture, fisheries management, and conservation. Scope: This journal publishes high-quality articles dedicated to all aspects Aquaculture, Fish biodiversity, Fisheries management, Fish diseases, Fishery biotecnology, Moleculer genetics, Fish health management, Fish biodiversity.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 8 Documents
 1 
Search results for , issue "Vol 20 No 1 (2020): February 2020" : 8 Documents clear
Cold temperature shock tetraploidization of striped catfish Pangasianodon hypophthalmus (Sauvage, 1878) with different of temperature and age of zygote Alfis Syahril; Odang Carman; Dinar Tri Soelistyowati
Jurnal Iktiologi Indonesia Vol 20 No 1 (2020): February 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i1.454

Abstract

Striped catfish (Pangasianodon hypophthalmus) has a relatively slow growth, so the operational cost of production is high and incomparable with the selling price, thus makes the cultivation of striped catfish is inefficient. Genetic quality improvement through polyploidization is to produce sterile triploid fish (3n) that can overcome this problem. The provision of triploid fish is more efficient through tetraploidization. Tetraploidization in striped catfish using cold shock treatment has never been done before. The purpose of this study was to determine the optimum tetra-ploidization condition using cold shock with temperature and different age of zygote in striped catfish. A completely randomized factorial design with two treatments i.e. three level of temperatures (8oC, 12°C, 16oC ) and two different ages of zygote (29 and 31 minutes after fertilisation-maf) and three replications was used. In addition, a control without applying temperature shock treatment was performed, Soaking process was conducted for 30 minutes. Tetraploid identification was done by calculating the maximum total of nucleoli per cell that was confirmed by calculating the total of chromosome. The results showed that the degree of hatching rate and abnormalities has a significantly different effect (P <0.05). Number of nucleoli per cell was 4 and chromosomes was 112 (4n=112) for tetraploid), whereas for diploid fish the maximum number of nucleoli per cell was 2 and the number of chromosomes was 56 (2n=56). The highest tetraploid percentage was obtained at a treatment of 12oC at age of zygote 29 maf. Thus, the optimum condition of tetraploidization in striped catfish is using cold shock treatment of 12oC with the age of zygote of 29 maf. Abstrak Ikan patin siam (Pangasianodon hypophthalmus) memiliki pertumbuhan yang relatif lambat sehingga biaya operasional produksi tinggi tidak sebanding dengan rendahnya harga jual yang mengakibatkan budidaya ikan patin siam tidak efisien. Perbaikan mutu genetik melalui poliploidisasi yaitu untuk menghasilkan ikan triploid (3n) yang bersifat steril dapat mengatasi masalah tersebut. Penyediaan ikan triploid lebih efisien melalui tetraploidisasi. Tetraploidisasi pada ikan patin siam menggunakan perlakuan kejutan dingin belum pernah dilakukan sebelumnya. Penelitian ini bertujuan untuk menentukan kondisi optimum tetraploidisasi menggunakan kejutan dingin dengan suhu dan umur zigot berbeda pada ikan patin siam. Rancangan percobaan pada penelitian ini adalah Rancangan Acak Lengkap faktorial dengan dua faktor dan tiga ulangan, yaitu suhu 8oC, 12oC,16oC dan umur zigot 29 dan 31 msf (menit setelah fertilisasi) dengan perendaman selama 30 menit dan satu perlakuan kontrol (tanpa pemberian kejutan suhu). Identifikasi tetraploid dilakukan dengan menghitung jumlah maksimum nukleolus per sel yang dikonfirmasi dengan penghitungan jumlah kromosom. Hasil penelitian menunjukkan bahwa derajat penetasan dan abnormalitas berbeda nyata (P<0,05). Hasil pengamatan pada ikan tetraploid diperoleh jumlah maksimum nukleoli adalah 4 per sel dan jumlah kromosom yaitu 112 (4n=112), sedangkan pada ikan diploid adalah 2 per sel dan jumlah kromosom yaitu 56 (2n=56). Persentase tetraploid tertinggi diperoleh pada perlakuan suhu 12oC umur zigot 29 msf. Disimpulkan bahwa kondisi optimum tetraploidisasi pada ikan patin siam menggunakan kejutan dingin pada suhu 12oC dengan umur zigot 29 msf.
Color quality and antioxidant activity evaluation of clown loach juvenile, Chromobotia macracanthus Bleeker 1852 by addition of astaxanthin in diet Rina Hirnawati; Dedi Jusadi; Jullie Ekasari; Muhammad Agus Suprayudi
Jurnal Iktiologi Indonesia Vol 20 No 1 (2020): February 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i1.492

Abstract

Botia or clown loach is the one of fishes was originated from Indonesia and as an ornamental fish commodity in the global market. Clown loach both nature and cultured have some problems when reared in aquaria that is the decrease of color quality. An innovation technology to improve color quality through diet formulates is needed. The aims of this research were to increase the color quality using astaxanthin in diet and to find out the role of astaxanthin as antioxidant in clown loach. The study consists of four treatments of astaxanthin dosages in the diet with isoprotein and isoenergy, namely 0, 50, 100 and 150 mg kg-1 with four replications. The fish juveniles with size of 3.44±0.51 cm and 0.56±0.02 g of weight with density of one fish per littre were used. The test diet was given three times a day during 60 days. The results showed that there were no significant differences antioxidant activity such as superoxide dismutase enzymes and malondialdehyde. However, there was a significant effect (P<0.05) to color quality of the chroma and total carotenoid, whereas no significantly different in lightness. The addition of astaxanthin was effective to improve the color quality of clown loach juvenile. Thus, 100 mg kg-1 of astaxanthin in diet is an optimal dose for clown loach juvenile. Abstrak Ikan botia adalah salah satu jenis ikan hias asli Indonesia dan merupakan komoditas perdagangan internasional. Ikan botia hasil tangkapan maupun hasil budidaya memiliki permasalahan saat dipelihara karena mengalami penurunan kualitas warna. Inovasi teknologi teknologi diperlukan untuk peningkatan kualitas warna ikan botia melalui ramuan pakan. Penelitian ini bertujuan untuk mengevaluasi pengaruh penambahan astaksantin dalam pakan terhadap kualitas warna dan peran astaksantin sebagai antioksidan pada yuwana ikan botia. Penelitian terdiri atas empat perlakuan de-ngan empat ulangan. Perlakuan berupa penambahan astaksantin dalam pakan dengan dosis yang berbeda yaitu 0, 50, 100, dan 150 mg kg-1. Pakan dibuat isoprotein dan isoenergi. Yuwana ikan botia yang digunakan berukuran 3,44±0,51 cm dan 0,56±0,02 g dengan padat tebar satu ekor per liter. Masa pemeliharaan selama 60 hari dengan frekuensi pemberian pakan tiga kali sehari secara at satiation. Hasil penelitian menunjukkan bahwa penambahan astaksantin tidak berpengaruh nyata terhadap kecerahan dan aktivitas antioksidan baik enzim superoxide dismutase maupun malondialdehyde, namun berpengaruh nyata (P<0,05) terhadap kepekatan warna dan karotenoid total. Penambahan astaksantin dalam pakan efektif untuk meningkatan kualitas warna ikan botia. Dosis astaksantin100 mg kg-1 dalam pakan merupakan dosis optimal bagi yuwana ikan botia.
Microplastics abundance in four different species of commercial fishes in Bali Strait Dara Sarasita; Agung Yunanto; Defri Yona
Jurnal Iktiologi Indonesia Vol 20 No 1 (2020): February 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i1.508

Abstract

Plastic wastes in marine environment will be degraded for long period of time to become microplastics. The small size of microplastics could be ingested by marine species which has lead to harmful impacts not only to the marine species but also to human. This research aimed to investigate microplastics ingestion in four commercial fishes in the Bali Strait, namely largehead hairtail (Trichiurus lepturus), Indian scad (Decapterus ruselli), Bali sardinella (Sardinella lemuru), and Indian mackerel (Rastrelliger kanagurta). Thirty fish samples from each species of fishes were obtained from the fishermen. Microplastics contents were identified visually using microscope from the gastrointestinal tracks of the fish and polymer of microplastics were identified using Fourier Transform Infra-Red (FTIR) test. The results showed that three types of microplastics (fiber, film and fragment) were found in the four species of fishes with different total abundances. The highest abundance was found in Bali sardine (7.03±0.49 particle individual-1), followed by Indian mackerel (5.03±0.50 particle individual-1), Indian scad (4.23±0.38 particle individual-1) and largehead hairtail (3.83±0.50 particle individual-1). FTIR test showed that polymer types of microplastic found were polyvinyl chloride (PVC), polyamide (PA), polycarbonate (PC), dan polyethylene (PE). Abstrak Sampah plastik dalam lingkungan laut akan terdegradasi dalam waktu yang sangat lama hingga menjadi mikroplastik. Ukuran mikroplastik yang sangat kecil dapat termakan oleh ikan dan membahayakan tidak hanya bagi ikan tersebut namun juga bagi manusia yang mengonsumsinya. Tujuan penelitian ini adalah untuk menganalisis keberadaan mikroplastik pada empat ikan ekonomis penting di perairan Selat Bali yaitu ikan layur (Trichiurus lepturus), layang (Decapterus russelli), lemuru (Sardinella lemuru) dan kembung (Rastrelliger kanagurta). Sebanyak 30 ekor ikan setiap spesiesnya didapatkan dari hasil tangkapan nelayan di Perairan Selat Bali pada Bulan Mei 2018. Kandungan mikroplastik pada saluran pencernaan ikan diidentifikasi jenisnya secara visual dan dengan menggunakan mikroskop serta uji Fourier Transform Infra-Red (FTIR) dilakukan untuk mengetahui polimer dari partikel mikroplastik tersebut. Terdapat tiga jenis mikroplastik yang ditemukan (film, fiber, dan fragmen) pada empat spesies ikan tersebut dengan kelimpahan jenis dan kelimpahan total yang berbeda-beda. Kelimpahan total mikroplastik tertinggi ditemukan pada ikan lemuru (7,03±0,49 partikel individu-1) diikuti oleh ikan kembung (5,03±0,50 partikel individu-1), ikan layang (4,23±0,38 partikel individu-1) dan ikan layur (3,83±0,50 partikel individu-1). Hasil uji FTIR menunjukkan sampel mikroplastik yang ditemukan terdiri dari beberapa polimer seperti polyvinyl chloride (PVC), polyamide (PA), polycarbonate (PC), dan polyethylene (PE).
Species composition and size distribution of sharks and rays caught in Bali Strait and its surrounding area and its relation to fisheries management Selvia Oktaviyani; Wanwan Kurniawan; Fahmi Fahmi
Jurnal Iktiologi Indonesia Vol 20 No 1 (2020): February 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i1.509

Abstract

Muncar is one of the shark production centers in Java, where the fishing ground in Bali Strait and its surrounding area. Routine data collection was carried out from May 2018 to April 2019 at the Brak Fish Market and the Muncar Coastal Fishing Port (PPP), Banyuwangi to determine the species composition and size distribution of sharks and rays caught in Bali Strait and its surrounding area. Data collection was done by census and almost of all individuals were identified to species level and and measured. During the study, 3.551 individuals of sharks and rays were landed in this port, consisting of 75 species from 25 families (48 species of sharks, one species of ghost shark and 26 rays species). The most common types of sharks belong to Carcharhinidae and for the rays was Dasyatidae. Among these, thirteen species were listed in Appendix II CITES, i.e. Carcharhinus falciformis, Alopias pelagicus, A. superciliosus, Isurus oxyrhinchus, I. paucus, Sphyrna lewini, S. zygaena, Mobula mobular, M. tarapacana, M. thrustoni, Glaucostegus typus, Rhynchobatus australiae and Rhyna ancylostoma. Most of sharks and rays caught were juvenile to adolescent and at immature or maturing stages. Abstrak Muncar merupakan salah satu sentra produksi hiu di Pulau Jawa dengan daerah tangkapan di perairan Selat Bali dan sekitarnya. Pendataan secara rutin terhadap hasil tangkapan hiu dan pari dilakukan sejak Bulan Mei 2018 hingga April 2019 di Pasar Ikan Brak dan Pelabuhan Perikanan Pantai (PPP) Muncar, Banyuwangi untuk mengetahui kom-posisi spesies dan distribusi ukuran hiu dan pari yang tertangkap dari perairan Selat Bali dan sekitarnya. Pengumpulan data dilakukan secara sensus dan hampir seluruh individu diidentifikasi sampai tingkat spesies serta diukur. Tercatat 3.551 individu hiu dan pari yang didaratkan di wilayah tersebut selama kurun waktu penelitian, yang terdiri atas 75 spesies dan 25 famili (49 spesies hiu dan 26 spesies pari). Spesies hiu yang paling umum ditangkap berasal dari famili Carcharhinidae, sedangkan kelompok pari didominasi oleh famili Dasyatidae. Tercatat ada 13 spesies hiu dan pari Apendiks II CITES yang ditangkap oleh nelayan Muncar, yaitu Carcharhinus falciformis, Alopias pelagicus, A. superciliosus, Isurus oxyrinchus, I. paucus, Sphyrna lewini, S. zygaena, Mobula mobular, M. tarapacana, M. thrustoni, Glaucostegus typus, Rhynchobatus australiae dan Rhina ancylostoma. Sebagian besar hiu dan pari yang ditangkap nelayan berada pada ukuran yuwana hingga remaja, yang belum matang kelamin atau sedang menuju dewasa.
Production of albino slayer through a combination of crossing in brushmouth (Hypostomus plecostomus, Linnaeus 1758) ornamental fish Ahmad Teduh; Dinar Tri Soelistyowati; Odang Carman; Harton Arfah
Jurnal Iktiologi Indonesia Vol 20 No 1 (2020): February 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i1.510

Abstract

The objectives of this research was to evaluate the inheritance of albino slayer phenotype in brushmouth (Hypostomus plecostomus, Linn 1758) through the crosses and reproduction performance. The crossing scheme consisted of reciprocal crosses between albino slayer and albino non slayer (SN, NS) and between albino slayer (SS), each spawning in pairs (1:1) with three replications and twice spawning. The fish used in this study were brushmouth fish (Hypostomus sp.) albino Slayer males 9.07 ± 0.5 cm, albino slayer females 8.96 ± 0.4 cm, albino non slayer males 8.87 ± 0.17 cm, and albino non slayer female 8.86 ± 0.16 cm. Slayer phenotype category was identified based on the caudal length fin with 4.43 ± 0.64 cm. Parameters determined included the phenotype distribution of albino slayer, total number of eggs, fertilization rate, hatching rate and survival rate. The results showed that all crossing schemes produced four phenotypes classes including normal slayer, normal non slayer, albino slayer and albino non slayer. The slayer phenotype of albino brushmouth was highest percentage (75%) in crossing with male albino slayer (SN, SS) and highest number of eggs, while the reciprocal crossing with female albino slayer (NS) produced albino slayer 55% and lower egg count. The ratio of tail fin length to body length in albino slayer phenotype was lower than normal phenotype of broom fish. The fertilization and hatching rates were not significantly different in all crosses; however the number of eggs decreased in the second spawning. The similar phenotype cross between albino slayer phenotypes (SS) resulted the best average of survival rate (92%). Abstrak Muncar merupakan salah satu sentra produksi hiu di Pulau Jawa dengan daerah tangkapan di perairan Selat Bali dan sekitarnya. Pendataan secara rutin terhadap hasil tangkapan hiu dan pari dilakukan sejak Bulan Mei 2018 hingga April 2019 di Pasar Ikan Brak dan Pelabuhan Perikanan Pantai (PPP) Muncar, Banyuwangi untuk mengetahui kom-posisi spesies dan distribusi ukuran hiu dan pari yang tertangkap dari perairan Selat Bali dan sekitarnya. Pengumpulan data dilakukan secara sensus dan hampir seluruh individu diidentifikasi sampai tingkat spesies serta diukur. Tercatat 3.551 individu hiu dan pari yang didaratkan di wilayah tersebut selama kurun waktu penelitian, yang terdiri atas 75 spesies dan 25 famili (49 spesies hiu dan 26 spesies pari). Spesies hiu yang paling umum ditangkap berasal dari famili Carcharhinidae, sedangkan kelompok pari didominasi oleh famili Dasyatidae. Tercatat ada 13 spesies hiu dan pari Apendiks II CITES yang ditangkap oleh nelayan Muncar, yaitu Carcharhinus falciformis, Alopias pelagicus, A. superciliosus, Isurus oxyrinchus, I. paucus, Sphyrna lewini, S. zygaena, Mobula mobular, M. tarapacana, M. thrustoni, Glaucostegus typus, Rhynchobatus australiae dan Rhina ancylostoma. Sebagian besar hiu dan pari yang ditangkap nelayan berada pada ukuran yuwana hingga remaja, yang belum matang kelamin atau sedang menuju dewasa.
Records of the rare deep-sea threetooth puffer fish, Triodon macropterus (Tetraodontiformes: Triodontidae) from Molucca and Sawu seas, Indonesia Kunto Wibowo; Miftahul Huda; Rachmad Sholeh Wicaksono; Monica Bataona; Teguh Peristiwady
Jurnal Iktiologi Indonesia Vol 20 No 1 (2020): February 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i1.511

Abstract

The Indo-West Pacific species of rare deep-sea threetooth puffer fish, Triodon macropterus Lesson 1931 was recorded on the basis of two voucher specimens from southern coasts of Lembeh Island, North Sulawesi, Molucca Sea and Solor Island, Sawu Sea and a photograph of fresh individual after captured from Sawu Sea. In Indonesia waters, the species has previously been known only from Banda Sea, thus the specimens and a photograph in this study represent the first records of T. macropterus from Molucca Sea and Sawu Sea. Abstrak Spesies ikan langka laut dalam Triodon macropterus Lesson 1831 yang mempunyai distribusi di perairan Indo Pasifik Barat, dijumpai di perairan lepas pantai selatan Pulau Lembeh, Sulawesi Utara, Laut Maluku dan lepas pantai selatan Pulau Solor, Laut Sawu, Indonesia berdasarkan identifikasi dari dua koleksi ilmiah spesimen dan sebuah foto individu sesaat setelah tertangkap. Di perairan Indonesia, spesies ini sebelumnya hanya diketahui tercatat dari Laut Banda, sehingga spesimen dari selatan Pulau Lembeh dan selatan Pulau Solor serta koleksi foto dalam studi ini merupakan catatan pertama distribusi T. macropterus dari Laut Maluku dan Laut Sawu.
Reproduction aspect of green swordtail, Xiphophorus hellerii Heckel 1848 in Tamblingan Lake, Bali I Nyoman Yoga Parawangsa; M. Fadjar Rahardjo; Charles P. H. Simanjuntak
Jurnal Iktiologi Indonesia Vol 20 No 1 (2020): February 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i1.512

Abstract

Eco-biological information of green swordtail in Tamblingan Lake is unknown. This research aimed to reveal sex ratio, gonadosomatic index, viviparity, spawning time and location of green swordtail in Tamblingan Lake. Fish was collected by gill nets with a mesh size of 0.5 and 1.0 cm in five sampling stations from January to June 2019. Total length and body weight of all fish samples were measured then dissected to observe the level of gonad maturity and viviparity in female fish. A total of 373 green swordtail with total length ranged and body weight ranged from 30.4-69.2 mm and 0.7935 – 7.6612 g, respectively. The results showed that males outnumberd of females in all sampling periods. Gonadosomatic index of male and female ranged from 0.159-1.239 and 0.204-13.592, respectively. The mature individuals were captured in all sampling stations and observation times. This species has a good reproductive potential with viviparity ranges between 6-54 juveniles. Abstrak Informasi ekobiologi ikan ekor pedang di Danau Tamblingan belum pernah ada. Penelitian ini bertujuan mengungkap nisbah kelamin, indeks kematangan gonad, viviparitas serta waktu dan lokasi pemijahan ikan ekor pedang di Danau Tamblingan. Ikan ditangkap menggunakan jaring insang dengan ukuran mata jaring 0,5 dan 1,0 cm di lima stasiun pengambilan contoh dari bulan Januari-Juni 2019. Seluruh contoh ikan diukur panjang dan ditimbang bobotnya lalu dibedah untuk melakukan pengamatan terhadap tingkat kematangan gonad serta jumlah telur pada ikan betina. Sebanyak 373 ikan ekor pedang ditemukan selama penelitian dengan kisaran panjang total 30,4-69,2 mm dan bobot 0,7935 - 7,6612 g. Hasil analisis menunjukkan bahwa proporsi jumlah ikan jantan lebih banyak daripada ikan betina pada setiap bulan pengamatan. Indeks kematangan gonad ikan jantan berkisar antara 0,155-1,239 dan ikan betina antara 0,203-13,592 . Ikan yang matang gonad ditemukan di seluruh stasiun pengambilan contoh pada setiap bulan pengamatan. Ikan ini memiliki potensi reproduksi yang baik dengan viviparitas antara 6-54 anak.
Preliminary study of closed system transportation, rearing and observation of the eggs Oryzias javanicus (Bleeker 1854) from Tunda Island Muh Herjayanto; Mas Bayu Syamsunarno; Nugroho Agung Prasetyo; Annisa Misykah Mauliddina; Lukman Anugrah Agung; Esa Rama Widiyawan; Novita Rahmayanti; Novitasari Irianingrum; Etin Nurkhotimah; Abdul Gani; Vianka Nafisa Salsabila
Jurnal Iktiologi Indonesia Vol 20 No 1 (2020): February 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i1.513

Abstract

Wild Oryzias javanicus from Tunda Island has potential as a native Indonesian aquatic ornamental fish and a model for research in the laboratory. The preliminary stage for raising wild fish is transporting from nature to the aquacul-ture environment. Generally, to minimize fish stress during transport in a closed system, anesthetics are added to the transport media. Furthermore, good adaptation in the aquaculture environment will lead to the process of reproduction as the ultimate goal of domestication of wild fish. The research objective is to analyze the transportation of closed systems and the rearing of O. javanicus post-transportation from Tunda Island. Research observations were carried out on behavior, survival, the number of eggs, and male: female sex ratio. Transport using an additional anesthetic treatment of 0.4 mL L-1 and without anesthesia, with fish density of 16 L-1. Anesthesia used is the commercial product Ocean Free® Special Arowana Stabilizer. Transportation is carried out for 11 hours. After that, rearing post-transportation is carried out for 16 days. The results showed that the transportation of O. javanicus can use a closed system without the addition of anesthesia with a density of 16 L-1 for 11 hours. In post-transport maintenance, fish begin active swimming on day 5, swim in groups continuously on day 6, start responding to natural food on day 3 and artificial feed on day 7, and produce a final survival of 91.67%. During maintenance, O. javanicus produces 104 eggs, 0.94 ± 0.06 mm in diameter and has an attaching filaments and a non-attaching filaments in the chorion. Oryzias javanicus is an egg depositor that can spawn with a 1: 1 and 1: 2 sex ratio. Abstrak Oryzias javanicus liar asal Pulau Tunda memiliki potensi sebagai ikan hias akuaskap asli Indonesia dan model untuk penelitian di laboratorium. Tahap awal untuk memelihara ikan liar yaitu melakukan pengangkutan dari alam ke lingkungan budi daya. Umumnya, untuk meminimalkan stres ikan selama pengangkutan sistem tertutup, dilakukan penambahan anestesi ke media pengangkutan. Selanjutnya, adaptasi yang baik dalam lingkungan budidaya, akan menyebabkan terjadinya proses reproduksi sebagai tujuan akhir domestikasi ikan liar. Tujuan penelitian yaitu meng-kaji pengangkutan sistem tertutup dan pemeliharaan pascapengangkutan O. javanicus asal Pulau Tunda. Pengamatan penelitian meliputi tingkah laku, sintasan, jumlah telur dan nisbah kelamin jantan : betina. Pengangkutan mengguna-kan perlakuan penambahan anestesi 0,4 mL L-1 dan tanpa anestesi dengan kepadatan 16 ekor L-1. Anestesi yang digunakan yaitu produk komersial Ocean Free® Special Arowana Stabilizer. Pengangkutan dilakukan selama 11 jam. Setelah itu, dilakukan pemeliharaan selama 16 hari. Hasil penelitian menunjukkan bahwa pengangkutan O. javanicus dapat menggunakan sistem tertutup tanpa penambahan anestesi dengan kepadatan 16 ekor L-1 selama 11 jam. Pada pemeliharaan pascapengangkutan, ikan mulai aktif berenang pada hari ke 5, berenang berkelompok secara kontinu pada hari ke 6, mulai merespons pakan alami pada hari ke 3 dan pakan buatan pada hari ke 7, dan menghasilkan sintasan akhir sebesar 91,67%. Selama pemeliharaan, O. javanicus menghasilkan 104 butir telur, diameter 0,94±0,06 mm dan dilengkapi filamen dan fili pada korion. Oryzias javanicus adalah egg depositor yang dapat memijah dengan nisbah kelamin 1:1 dan 1:2.

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