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<![CDATA[Sintesis Fenil Sinamat dan 4-Fenilkroman-2-on dan Uji Sitotoksisitas Terhadap Sel Kanker Serviks HeLa ]]>
<![CDATA[TENI ERNAWATI]]>;
<![CDATA[DILA FAIRUSI]]>
<![CDATA[ JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI ]]>
Publisher : <![CDATA[Fakultas Farmasi Universitas Pancasila ]]>
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<![CDATA[Cinnamic acid is a derivative of methyl cinnamate; it is included in the shikimic acid pathway derivatives. Cinnamic acid and its natural analogue are known as a cancer treatment for several centuries.Synthesis of phenyl cinnamic and 4-phenilkroman-2-on derived from methyl cinnamate had been done by using acid as a catalyst. Methyl cinnamic was first converted into cinnamic acid via hydrolysis reaction with alkaline. After that, cinnamic acid was chlorinated by using thionyl chloride and phenol reagent to produce fenil-sinamat. Esterification reaction was carried out by using an acid catalyst p-toluen sulfonic acid and phenol resulting 4-phenilkroman-2-on. We obtained the products for the synthesis of cinnamic acid, cinnamic phenyl and 4-fenilkroman-2-on, with yields respectively of 83.6%, 14.71%, and 16.18%.Cytotoxicity test using the brine shrimp lethality test (BSLT) was conducted on both cinnamic phenyl compound and 4-phenilkroman-2-on compound. LC50 values obtained for both compounds respectively were 223.87 ppm and 112.72 ppm. The results of MTT cytotoxicity assay method for cervical cancer HeLa cells (ATCC CCL2) using phenyl cinnamic and 4-phenilkroman-2-on were found that the percentage of inhibition obtained is over 50%.]]>
<![CDATA[Formulasi dan Efektivitas Gel Antiseptik Tangan Minyak Atsiri Lengkuas (Alpinia galanga (L.) Willd.) ]]>
<![CDATA[BANU AJI WIJAYANTO]]>;
<![CDATA[DHADHANG WAHYU KURNIAWAN]]>;
<![CDATA[ISKANDAR SOBRI]]>
<![CDATA[ JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI ]]>
Publisher : <![CDATA[Fakultas Farmasi Universitas Pancasila ]]>
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<![CDATA[The use of antiseptic hand gel with regards to paradigm that is healthy as well as clean practical, as an attempt to avoid the onset of infection. The volatile oil of Alpinia galanga rhizomes are known active as antibacterial. Three formulation of gels containing of 1 mL of galangal oil were made with variations of carbopol 940 from 0.5% - 2 %. Antiseptic test were done using replica methods. Data of viscosity, spread power, and adhesion were analyzed using one way ANAVA. Data of effectiveness was analyzed using one way ANAVA and if there is any differences, it will be continued by using HSD Tukey. The results showed that viscosity of gels were pseudoplastic flows. Spread power of gel preparations increased, adhesion and viscosity decreased during storage. All gel preparation showed the effectiveness of antiseptic gels because be able to reduce the number of bacterial colonies. Effectiveness of antiseptic formula that contains of carbopol 0.5% can reduce the number of colonies 70% and did not differ from the positive control which can reduce the number of colonies up to 79.37%.]]>
<![CDATA[Homology Modeling dan Molecular Docking Senyawa Aktif dari Bengkoang (Pachyrrhizus erosus) sebagai Inhibitor Tirosinase pada Homo sapiens ]]>
<![CDATA[ENDANG LUKITANINGSIH]]>;
<![CDATA[ANNY A. MUSTIKAWATY]]>;
<![CDATA[ARI SUDARMANTO]]>
<![CDATA[ JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI ]]>
Publisher : <![CDATA[Fakultas Farmasi Universitas Pancasila ]]>
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<![CDATA[One mechanism of whitening agent is to inhibit the tyrosinase enzyme in melanogenesis process. However, since the human tyrosinase has not been determinated experimentally, therefore research on human tyrosinase can not be conducted. Homology modeling is one method that can be used to exhibit the three-dimensional structure of human tyrosinase. This model used to predict the interaction of bengkoang (Pachyrrhizus erosus) active compounds with the human tyrosinase enzyme by using a molecular docking approach (pose analysis). In addition, the strength of the bond between the ligand compounds and the site protein target can be predicted by analyzing the resulting score. Homology modeling was carried out using Amber99 method of software MOE 2009.10 and Bacillus megaterium (TyrBm) as a template. The model obtained was evaluated using Ramachandran plot analysis, z-score, and the plot energy ProSA. The best model used for molecular docking using the active site ligand, ALPHA PMI placement method, and affinity DG scoring of MOE 2009.10 software. The best model is the TyrHSM (MOE default alignment) with 96.7% residues are in allowed regions of the Ramachandran plot, has the least positive region, and has a z-score ratio> 1. The isolated compound 2-butoxy-2,5-bis (hydroxymethyl)-tetrahydrofurane-3,4-diol is predicted have the best affinity to human tyrosinase with a score -0.3598 kcal/mol.]]>
<![CDATA[Senyawa Imunomodulator dari Myrmecodia pendens Merr & Perry ]]>
<![CDATA[TRIANA HERTIANI]]>;
<![CDATA[MARIA ULFAH]]>;
<![CDATA[EDIATI SASMITO]]>
<![CDATA[ JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI ]]>
Publisher : <![CDATA[Fakultas Farmasi Universitas Pancasila ]]>
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<![CDATA[Myrmecodia pendens Merr & Perry (Rubiaceae) is widely used in Papua as traditional medicine against various diseases. Preliminary study revealed that ethyl acetate fraction has potency to increase mice Balb/c lymphocyte proliferation in vitro. This study aimed to investigate the active compound responsible for the activity. Dried powders of plant tubers were macerated in ethanol 95% followed by solvent evaporation. The crude extract was further fractionated by liquid-liquid partition with n-hexane, ethyl acetate and water, successively. The ethyl acetate fraction was subjected to a silica gel column and separated by vacuum. The subfraction yields were tested on mice Balb/c lymphocytes to observe the influence on lymphocyte proliferation by MTT assay. Total phenolic content of subfractions were determined by Folin-Ciocalteau method. Chemical characteristics of active compound was identified by GC-MS, UV-Vis spectroscopy, IR and H-NMR. The result showed that total phenolic content has no significant correlation to the lymphocyte proliferation activity. Subfraction 7 yielded from ethyl acetate-methanol (7:3v/v) contained a major constituent (78% by GCMS, 83.75% by Densitometry). GCMS and UV spectra suggested a phenolic substructure. IR and H-NMR suggested that the phenolic was glycosilated and had aliphatic moiety.]]>
<![CDATA[Uji Aktivitas Fagositosis Makrofag Fraksi-fraksi dari Ekstrak Metanol Daun Sirih Merah (Piper crocatum Ruiz & Pav.) Secara In Vitro ]]>
<![CDATA[YUSTINA SRI HARTINI]]>;
<![CDATA[SUBAGUS WAHYUONO]]>;
<![CDATA[SITARINA WIDYARINI]]>;
<![CDATA[AGUSTINUS YUSWANTO]]>
<![CDATA[ JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI ]]>
Publisher : <![CDATA[Fakultas Farmasi Universitas Pancasila ]]>
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<![CDATA[Plants can be sources of immunomodulatory agents. Piper crocatum Ruiz & Pav. (red betel) leaves have been used to heal many diseases, especially to increase endurance. The aim of this research was to investigate the imunomodulatory effect of methanolic leaf extracts of Piper crocatum Ruiz & Pav. and its fractions by in vitro phagocytic macrophage activity test. The result showed that methanolic extract of Piper crocatum Ruiz & Pav. 75μg/mL could stimulate phagocytic macrophage activity equal to 100μg/mL product-X® that contained Echinacea extract. Separation of the extract by vacuum liquid chromatography using gradient solvent n-hexane - ethyl acetate yielded 5 fractions. The second fraction showed the highest activity. It contained alkaloid and terpenoid, while the extract also contained flavonoid and essential oil. Alkaloid and/or terpenoid could be the compound in the leaves of Piper crocatum Ruiz & Pav. that responsible increasing phagocytic macrophage activity.]]>
<![CDATA[Efek Stimulansia Infus Lada Hitam (Piperis nigri fructus) Pada Mencit ]]>
<![CDATA[ROS SUMARNY]]>;
<![CDATA[LESTARI RAHAYU]]>;
<![CDATA[NI MADE DWI SANDHIUTAMI]]>;
<![CDATA[LIBERT MORY]]>
<![CDATA[ JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI ]]>
Publisher : <![CDATA[Fakultas Farmasi Universitas Pancasila ]]>
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<![CDATA[Black pepper (Piperis nigri fructus) has been empirically used to eliminate fatigue and to increase stamina or as stimulant. The aim of this study was to determine the efficacy of black pepper infuse as stimulant on 30 male DDY mice using swimming and rotarod methods. The mice were divided into five groups. First group was given distilled water as control, second group was given caffeine of 0.36 mg/20 g bw. The other three groups were given orally with black pepper infuse of 0.32 mg/20 g bw, 0.65 mg/20 g bw, or 1.3 mg/20 g bw. Observations on mice swimming endurance and mice endurance on a rotarod, were carried out 60 minutes after oral administration of the solutions. Analysis using Kruskal-Wallis showed that there was significant difference in the stimulant efficacy between the black pepper infuse and the negative control (p<0.05). There was no significant difference in stimulant effect between the 0.32 mg/20 g bw of black pepper infuse and the caffeine positive control, however, the infuse dose of 0.65 mg/20 g bw and 1.3 mg/20 g bw have demonstrated stronger stimulant effects (p>0.05) as compared to the caffeine positive control.]]>
<![CDATA[Efektivitas Krim Anti Jerawat Kayu Secang (Caesalpinia sappan) Terhadap Propionibacterium acnes pada Kulit Kelinci ]]>
<![CDATA[SITI SA’DIAH]]>;
<![CDATA[LATIFAH KOSIM DARUSMAN]]>;
<![CDATA[WULAN TRIWAHYUNI]]>;
<![CDATA[IRMANIDA BATUBARA]]>
<![CDATA[ JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI ]]>
Publisher : <![CDATA[Fakultas Farmasi Universitas Pancasila ]]>
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<![CDATA[Sappan Wood extract (Caesalpinia sappan) is potential as an anti-acne due to its ability as an antioxidant, lipase inhibitor and anti Propionibacterium acnes. The potency is influenced by the quality of simplicia and extracts. To elevate the application, semisolid dosage formulations were made in cream forms using a standardized extract of Sappan Wood. Creams were formulated with the same base composition but different extract concentrations (range of 1-10%). In vivo assay were carried out on rabbit skin induced by intradermal injection of P. acnes. Sappan Wood was collected from Semarang and the resulting extract has met the Materia Medika Indonesia requirement with brazilin level in extract of 224.675 mg / g. In vivo tests showed that all Sappan Wood extract formulations have potency as anti-acne as indicated by curative percentage for 15 days were significantly different from the formula base. Curative effect of all formula were not significantly different with the Mediklin® positive control. Formula with the lowest concentration of the extract was the most effective and efficient with the curative percentage of more than 96% in 15 days.]]>
<![CDATA[Uji Preklinis 177Lu-DOTA-Trastuzumab: Radiofarmaseutika Potensial untuk Terapi Kanker Payudara HER-2 Positif ]]>
<![CDATA[MARTALENA RAMLI]]>;
<![CDATA[BASUKI HIDAYAT]]>;
<![CDATA[SRI AGUSWARINI]]>;
<![CDATA[KARYADI KARYADI]]>;
<![CDATA[CAHYA NOVA ARDIYATNO]]>;
<![CDATA[HAMMAD SUBUR]]>;
<![CDATA[TITIS SEKAR HUMANI]]>;
<![CDATA[RIEN RITAWIDYA]]>;
<![CDATA[ABDUL MUTALIB]]>;
<![CDATA[JOHAN MASJHUR]]>
<![CDATA[ JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI ]]>
Publisher : <![CDATA[Fakultas Farmasi Universitas Pancasila ]]>
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<![CDATA[Radiopharmaceutical 177Lu-(1,4,7,10-tetraazacyclododecane-N,N’,N”,N”’-tetra acetic acid)-trastuzumab (177Lu-DOTA-trastuzumab), based on antihuman epithelial receptor type 2 (HER-2) monoclonal antibody which is expected to be potential for diagnostic and therapeutic agent of breast cancer positive HER-2, had been successfully prepared with radiochemical purity of > 99%. Preclinical studies aimed in providing basic data for clinical trial and particularly in finding out the effectiveness of 177Lu-DOTA-trastuzumab in killing cancer cells which over express HER-2, have been performed. The data included urine and faeces clearance tests, imaging with gamma camera and cytotoxicity test. The results showed that the excretion of radioactivity post injection of 177Lu-DOTA-trastuzumab in normal rats were more rapid through urine as compared to the excretion through faeces. The gamma camera image on normal rat 144 hours post injection of 177Lu-DOTA-trastuzumab showed that there was remaining a trace of radioactivity in hepatic area. The residue of radioactivity (< 5%, quantified by biodistribution test) was found to be relatively lower than reported for 111In-NSL-trastuzumab. However, this residue of radioactivity has to be seriously considered when 177Lu-DOTA-trastuzumab is going to be applied for treatment of cancer. Cytotoxicity test showed that 177Lu-DOTA-trastuzumab was far more effective in killing cancer cells positive HER-2 (SKOV-3 cell lines) than that of trastuzumab.]]>
<![CDATA[Aktivitas Antimikroba dan Analisis Gen Plantarisin F dari Isolat Lactobacillus Asal Buah-buahan Tropis ]]>
<![CDATA[TITIN YULINERY]]>;
<![CDATA[NOVIK NURHIDAYAT]]>
<![CDATA[ JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI ]]>
Publisher : <![CDATA[Fakultas Farmasi Universitas Pancasila ]]>
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<![CDATA[Lactobacillus isolated from tropical fruits can be used as probiotic and potential to prevent diseases in the digestive tract due to bacteriocin content. One of the bacteriocin called plantaricin F, is produced by Lactobacillus plantarum. Plantarisin F has antimicrobial activity against Gram positive and Gram negative bacteria. The aim of the research was to test the antibacterial activities of several Lactobacillus species on 5 bacteria isolates, and analyzed the gene expression of plantaricin F. Seven isolates of ten selected isolates could inhibit the growth of Gram positive and Gram negative bacteria. Lactobacillus TB (NK) produced the largest inhibition area of 2.05 cm on E.coli, and twice stronger than chloramphenicol control. As confirmation, the plantaricin F gene was analyzed using RT-PCR. The analysis result showed that the size of plantaricin F gene was 70 bp. The highest relative expression of plantaricin F gene was shown by the Lactobacillus Mar A2 isolate of 10,676 Au, followed by Lactobacillus TB (NK) of 2.9207 Au, and the lowest was Lactobacillus TB (CK) of 0.5141 Au. Lactobacillus TB (NK) can be used as candidate of probiotic and alternative antibiotic in preventing of gastrointestinal disease.]]>
<![CDATA[Pengembangan Getah Pepaya, Ekstrak Pepaya (Carica papaya L.) dan Ekstrak Umbi Bengkuang (Pachyrrhizus erosus (L.) Urb.) untuk Lotion Pencerah Kulit Berdasar Aktivitas Antioksidan dan Inhibisi Tirosinase ]]>
<![CDATA[LEONARDUS BROTO SUGENG KARDONO]]>;
<![CDATA[LIANDHAJANI LIANDHAJANI]]>;
<![CDATA[NINA ARTANTI]]>;
<![CDATA[YETI MULYATI ISKANDAR]]>;
<![CDATA[SITI MASRURAH BROTO SUTARYO]]>
<![CDATA[ JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI ]]>
Publisher : <![CDATA[Fakultas Farmasi Universitas Pancasila ]]>
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<![CDATA[Whitening or skin lightening products are well known as cosmetic. In this development, papaya latex (Carica papaya L.), papaya fruit extract and yam bean tuber extracts (Pachyrrhizus erosus (L.) Urb.) were combined for skin lightening body lotion formula. Antioxidant and tyrosinase inhibitory activities were evaluated in the starting materials and the products. Antioxidant activity evaluation was conducted using DPPH free radical scavenging activity method. Tyrosinase inhibitory evaluation was performed based on tyrosinase enzymatic inhibitory reaction to tyrosine as substrate. Papaya latex showed the highest antioxidant activity (32.01%), followed by yam bean tuber extract (27.04%) and papaya extract (26.09%). The tyrosinase inhibitory activities of papaya latex, papaya fruit extract and yam bean tuber extracts were 68.42 %, 36.80% and 52.63 % respectively, as compared to that of arbutin (100%). Based on product appearance, color, odor, pH and viscosity, the product were stable for 128 weeks. The expired date calculation on its antioxidant activity, however, the product was stable for 77 weeks. It is recommended that the product should be stored in non transparent bottle at low temperature or at about 25-28oC.]]>
