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Aquacultura Indonesiana

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Published by Universitas Diponegoro

ISSN : 02160749 EISSN : 24776939 DOI : -

Core Subject : Science,

Earth & Planetary Sciences

Aquacultura Indonesiana (AI) is publishes original and peer-reviewed, English language papers concerned with culture of aquatic plants and animals. Subjects approriate for this journal would include, but not necessarily be limited to, nutrition, diseases, genetics and breeding, physiology, environmental quality, culture system enginering. husbandry practices, and economics and marketing. Fragmentary reports will not be considered for publication; coherent research should be published in a single paper. Preliminary studies, simple case reports, baseline data, parasite host or range extentions, and other such curiosities will not be considered for publication in the journal.

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Articles 83 Documents

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DNA Profile of Pacific White Shrimp, L. vannamei Infected by Taura Syndrome Virus Using Single Strand Conformation Polymorphism (SSCP) Analysis Permana, Gusti Ngurah; Moria, Sari Budi; haryanti, haryanti
Aquacultura Indonesiana Vol 17, No 1 (2016)
Publisher : Indonesian Aquaculture Society (MAI)

The shrimp infected by Taura syndrome virus (TSV) can survive until harvesting time. At this phase, the growth of this shrimp is relatively normal but it is referred as TSV carrier. This research is expected to provide a recommendation in effort to prevent the occurrence of TSV infection. Therefore, this study was aimed to evaluate the DNA polymorhism of Pacific white shrimp L. vannamei infected by TSV. A total of 60 samples of L. vannamei were collected from SPF (specific pathogen free)-Florida, F1-healthy, F1-tolerance (it is can life normally after infected by TSV), and F1-moribund (diying after infected by TSV). The samples were analysis using PCR with IQ-2000 KIT to determine the positive infection of samples. DNA profile of white shrimp, L. vannamei was significantly different from SPF Florida, F1-moribund, and F1-healthy shrimp. This expression may control the defense mechanism. This can be ascribed to the reason that shrimp survived from TSV infection had a higher amount of locus (3 loci) that were detected from DNA polymorphism of gene in white shrimp contributed to a gene control that was determined from allele variation. Two important alleles (200 bp and 220 bp) unique may be associated with tolerant trait. Those loci only found in F1-tolerance shrimp. However to prove that further necessary to sequences a nucleotide of three loci as responsible for resistance to TSV infection.

Growth, Total Production and Feed Efficiency of Catfish (Clarias sp.) Orally Administered with Shrimp Waste Hydrolyzate Hariyadi, Dimas Rizky; Isnansetyo, Alim; Istiqomah, Indah; Hardaningsih, Ign.; Wahyudi, Wahyudi; Kim, Sung Sam
Aquacultura Indonesiana Vol 19, No 1 (2018)
Publisher : Indonesian Aquaculture Society (MAI)

Shrimp Waste Hydrolyzate (SWH) /Shrimp Soluble Extract (SSE) is a product produced from the enzymatic process of shrimp waste. SWH contains essential and non-essential amino acids which useful for fish. This study aimed to determine effect of SWH on the growth and total production of catfish. The parameters observed were average growth rate, specific growth rate (SGR), food conversion ratio (FCR), protein efficiency ratio (PER), total production and length weight relationship from beginning until the end of rearing period. Growth performance was observed by calculating average growth rate and SGR of catfish. The results of this study showed that oral administration of SWH significantly increased SGR of catfish length at 4th sampling period with value 1.54% body length/day. Administration of SWH did not affect on the AGR, FCR, PER and total production. The growth pattern of catfish is negative allometric.

The Morphometric Character and Mitochondrial 16S rRNA Sequence of Portunus pelagicus Fujaya, Yushinta; Hidayani, Andi Aliah; Asphama, Andi Ivo; Trijuno, Dody Dharmawan; Tenriulo, Andi; Parenrengi, Andi
Aquacultura Indonesiana Vol 16, No 1 (2015): Volume 16 Issue 1 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

Blue swimming crab (Portunus pelagicus) colors have variations from different habitats and they have different vitality. The purpose of this study was to analyze the diversity of the P. pelagicus using morphometric and 16SrRNA mitochondrial sequence. There were three groups of blue swimming crab characterized by differences in color and density patterns of spots. The result of Canonical discriminant analysis showed that there were morphometric differences among the three groups. The main distinguishing morphometric characteristics are the ratio of carapace width and length as well as the ratio of the length and width of cheliped merus. The third group of crab is also has similarity about 98-99% by the Portunus pelagicus 16S rRNA sequences available in gene banks. These studies had concluded that differences in color and pattern of the spot density have also morphometric and genetic differences. At least there were two groups based on genetic variation of Portunus pelagicus from Barru Regency waters which may have a variety of different properties. Further research is needed to study the adaptation abilities of the two groups of crab on the condition of aquaculture as a potential domestic animal.Keywords: Crab; Portunus pelagicus; Morphometric; 16S rRNA.

Oral Administration of Alginate From A Tropical Brown Seaweed, Sargassum sp. To Enhance Non-Spesific Defense In Walking Catfish (Clarias sp.) Isnansetyo, Alim; Irpani, Husni Mubarok; Wulansari, Tyas Ayu; Kasanah, Noer
Aquacultura Indonesiana Vol 15, No 1 (2014): Volume 15 Issue 1 Year 2014
Publisher : Indonesian Aquaculture Society (MAI)

Alginate that is usually extracted from brown seaweeds, is a bioactive substance with immunomodulator activity. The purpose of this research was to evaluate the effect of oral administration of alginate on the non-specific immune system of walking catfish (Clarias sp.). An experiment was conducted in a Completely Randomized Design with five treatments in triplicates. Alginate was supplemented in feed at doses of 0 (control treatment), 2, 4, 6 and 8 g/kg of feed. The walking catfish was fed twice daily at feeding rate of 5%. Non-specific defense parameters evaluated were Nitroblue Tetrazolium (NBT) activity, phagocytic activity (PA), phagocytic index (PI), leukocyte differentiation, hematocrit and leucocrit. The parameters were observed prior to be treated, 5th, 10th and 15th days after treatments. Oral administration of alginate at 4 g/kg of feed increased NBT and PA activities significantly (P<0.05) in 5 days, while alginate at a dose of 6 g/kg increased NBT activity and monocyte percentage significantly (P<0.05) in 5 days. These results suggested that oral administration of alginate at 4 g/kg effectively increased non-specific immune system of walking catfish.

Water Quality Assessment Using Remote Sensing and GIS for In-shore Marine Environment Suitability Ruslisan, Ruslisan; Kalam, Nur H; Dwininta, Aglis C; Habibi, Muhammad H; Rahayu, Ernawati T; Dewi, Nur khovia; Henny K, Eleonora E; Widyatmanti, Wirastuti
Aquacultura Indonesiana Vol 17, No 2 (2016)
Publisher : Indonesian Aquaculture Society (MAI)

In-shore marine environment and its adjoining estuary has a potential susceptibility to water pollution due to the continuous discharge of its unhealthy catchment. Seawater quality studies commonly require a very detailed water sampling and analysis, leading to high expenditures on time and energy. This study aims to examine the water quality of the Dodokan Estuary using remote sensing and geographic information system (GIS) approaches, and to determine the most appropriate water environment mapping unit for marine environment suitability studies. Landsat 8 and QuickBird® were used to extract water quality parameters using related spectral transformations. Field surveys were conducted concurrently to the Landsat 8 acquisition time on the study area, to collect water samples for laboratory analysis including sea surface temperature (SST), chlorophyll-a, Dissolved Oxygen (DO), and Total Suspended Solid (TSS). Mapping units were generated based on visual interpretation on QuickBird® for estuarine plumes, to identify the possible distribution of suspended material from its catchment. The statistical analyses present that the parameter extracted from the satellite imagery and from laboratory analysis produces R values of 0.7 in average, despite its low value on chlorophyll-a. By utilizing assorted sea-water marine suitability criteria, it can be concluded that the inshore marine environment in the Dodokan Estuary and its surrounding area are suitable for various biota conservation e.g. coral, sea-grass, and mangrove, and in contrast, marine aquaculture. The knowledgeable uses of remote sensing and GIS also assist the sea-water qualities assessment mapping in term of providing boarder understanding of a water environment condition to effectively minimize the cost of sampling.

Isolation and Identification of Jellyfish Alkaloid (Bougainvillia Sp) as Immunostimulant to Profile of Protein and Fagosite Activity of Tiger Grouper (Epinephelus Fuscoguttatus) Andayani, Sri; Fadjar, M.; Rahman, M. Farid
Aquacultura Indonesiana Vol 18, No 2 (2017)
Publisher : Indonesian Aquaculture Society (MAI)

The aim of this research was to know the profil protein and phagocyte activity of tiger grouper (Epinephelus fuscoguttatus). The method used in this research was experimental method with Completely Randomized Design (RAL) consisting of 5 treatment levels ie. K=control group without giving alkaloids and feeding pellets mixed with Jellyfish alkaloid Bougainvillia sp., which were treatment A = 0.5 g, B = 0.75 g, C= 1 g and D = 1.25 g alkaloid/kg of feed conducted in 2 replications. It was challenge tested with Vibrio harveyi of 107 cfu/mL for 5 days. Blood plasma for protein profile and phagocyte activity was performed after giving immunostimulant on day 28 and after being infected on day 34. The results of the study were: (1) alkaloid characteristic was N-1 Benzylalcohol, 4-Octyl Piperidine) and (2) protein profile after immunostimulant addition resulted in 9 bands and sample after infection showed 7 protein bands. The phagocyte activity increases 11.4% to 80.6%. It is suggested to use 1 g of alkaloid/kg of feed as immunostimulant.

Fatty Acid Profile of Hepatopancreas and Oocyte of Tiger Shrimp, Penaeus monodon, Fed Modified-commercial Diet by Supplementing Vitamin C and E Usman, Usman; Kamaruddin, Kamaruddin; Laining, Asda; Trismawanti, Ike
Aquacultura Indonesiana Vol 20, No 1 (2019)
Publisher : Indonesian Aquaculture Society (MAI)

Vitamin C and E have important roles in the process of reproduction, immune system, and anti-oxidants to protect cell membrane lipid, and the circulation of essential fatty acid in the body of the aquatic organism including shrimp. The aims of this study was to analyze the effect of vitamin C and E supplementation in modified-commercial diet on the fatty acids profile of hepatopancreas and oocyte of tiger shrimp, Penaeus monodon. The experimental treatments were: modified-commercial diet by supplementing with vitamin C (500 mg/kg) and E (300 mg/kg) (PS), and modified-commercial diet without vitamins supplementation (PK). Cultured tiger shrimp were used as the animal with initial weight of 43.1 ± 5.1 g (mean±STD) for females and 41.9 ± 4.4 g for males. The shrimps were cultured in two of 1000 m2 concrete ponds with a density of 100 shrimp / pond. During three months culture in the pond, they were fed the test at rate of 3% biomass/day. When the tiger shrimp reached the weight over 90 g for females and > 70 g for males, then the shrimp were transferred to concrete tanks for oocyte maturation evaluation. In maturation tank, the shrimp fed test diets (pellet) combined with fresh food (squid) as much as 3% (dry basis) at combination ratio of 50% : 50%. Shrimp reaching oocyte development stage III were dissected and sampled for hepatopancreas and oocytes for analysis of fatty acid profile. The results showed that the hepatopancrea and oocyte fatty acids contents were higher in the tiger shrimp fed PS compared to the tiger shrimp fed PK. The poly unsaturated fatty acid (PUFA) especially for arachidonic, docosahexaenoic, and eicosapentaenoic acid were higher in shrimp fed with PS than the shrimp fed PK. Despite higher total fatty acid contents in hepatopancreas than in oocyte for the both treatments, the esensial fatty acid arachidonic, docosahexaenoic, and eicosapentaenoic acid were higher in oocyte than in hepatopancreas of the shrimp. This suggested that these three types of PUFA played important role for oocyte development for female reproduction. Supplementation of vitamin C and E in the test diet had an effect on increasing PUFA content in hepatopancreas and oocytes of tiger shrimp.

Water Quality and Productivity of Dryland Catfish Pond Based on Existing Technology and Land Priority in Gunungkidul Septriani, Nur Indah; Rustadi, Rustadi
Aquacultura Indonesiana Vol 16, No 2 (2015): Volume 16 Issue 2 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

This research is aimed to examine the water quality and productivity of dryland catfish pond based on the existing technology and land priority in Gunungkidul and to determine the suitable technology based on the land priority. Samples from the ponds employing intensive, extensive, and semi-intensive technology were analysed to find out the water quality such as the ammonia, nitrate, and nitrite contents; pH; DO; temperature and turbidity; land productivity; and profit. Existing analysis used scoring of sample ponds which included water quality, productivity and profit analysis. The highest score of the sample ponds becomes the sample of technology applied in the certain area. The highest score in high priority land was PTI (79%), medium priority land was PSS (72%), and non-priority land was NPE (68%). The water quality, productivity and profit in high priority land reached optimal result when it used intensive technology. The water quality, productivity and profit in medium priority land reached optimal result when it used semi-intensive technology. The water quality, productivity and profit in non-priority land reached optimal result when it used extensive technology.Keywords: Water quality; productivity; scoring; dryland catfish pond; existing technology; land priority

The Impact of Component Modification of “Double Bottom Filter” Recirculation System to Concentration of Ammonia in Water and Survival Rate at Rearing Media of Blue Devil Fish (Chrysiptera cyanea) F. Sahetapy, Jacqueline M; Louhenapessy, Daniel G; Riry, Elna C
Aquacultura Indonesiana Vol 17, No 1 (2016)
Publisher : Indonesian Aquaculture Society (MAI)

Blue devil (Chrysiptera cyanea), blue damselfish, is a variety of marine ornamental fish. In rearing ornamental fish, water quality management is required to maintain the optimal condition of water quality. Filtration and recirculation are proven as preeminent ways to manage the optimal condition of water quality in aquarium as the vessel for rearing ornamental fish. Simple recirculation system double bottom filter was applied in this research with treatments of components modification of the system. The objective of the research was to identify the concentration of ammonia in rearing media of blue devil (C. cyanea) using recirculation system double bottom filter with several modification components. The treatments are i.e. (1) no recirculation/aeration only (treatment A), (2) system with components of sand and dacron (treatment B), (3) sand and sponge (treatment C). The result showed that the ammonia concentration in system A (control) was 0.890 mg/L, system B (sand and dacron) was 0.129 mg/L, and system C (sand and sponge) was 0.7 mg/L. The highest value of survival rate was in system B (components sand and dacron) which valued 90%. As the conclusion, component sand and dacron in recirculation system double bottom filter provided better performance in reducing ammonia in rearing media of blue devil (C. cyanea) which also confirmed with the survival rate value.

Growth Performance of Eucheuma Cotonii by Immersing in Several Macroalgae Extract Cokrowati, Nunik; Setyowati, Dewi Nur’Aeni; Kurnianingsih, Rina
Aquacultura Indonesiana Vol 18, No 1 (2017)
Publisher : Indonesian Aquaculture Society (MAI)

Macro algae in West Nusa Tenggara are potential in types and quantities. One of the types that have only been economically used is seaweed, such as Eucheuma cottonii, Eucheuma spinosum, Eucheuma striatum and Gracillaria sp. In general, all macro algae can be used for various purposes such as pharmacy, agriculture, livestock, and food ingredient. Macro algae commonly have the growth hormones and contain primary and secondary metabolites. The growth hormones in macro algae are called cytokinin, auxin and absicic acids. The objective of this study was to determine the effect of macro algae extracts to the growth of Eucheuma cottonii. This study was carried out in Ekas Bay, Ekas Buana Village, Jerowaru District, of East Lombok, West Nusa Tenggara, between May to September 2016. Eucheuma cottonii was cultivated on the 8 m X 8 m size of longline and 40 ris of load. Spacing between ris was 1 m and distance between clumps was 25 cm. Cultivation was carried out by using Eucheuma cottonii seed with average weight of 100 g and weighing was carried out every 7 days. Macro algae used as extract included Sargassum aquifolium, Ulva sp. and Turbinaria sp. Extracts were put into immersion at 5% concentration. The parameters measured were the absolute growth and Specific Growth Rate of Eucheuma cottonii, and the water quality. The results showed that the addition of macro alga extracts significantly affected the growth of Eucheuma cottonii.

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