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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,183 Documents
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VANCOMYCIN RESISTANT STAPHYLOCOCCUS AUREUS IN DR. WAHIDIN SUDIROHUSODO HOSPITAL MAKASSAR Fatmawaty Ahmad; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1385

Abstract

Vancomycin Resistant Staphylococcus aureus (VRSA) refers to bacteria that have complete resistance to Vancomycin. The increasing prevalence of VRSA can be a significant clinical problem with the limited therapeutic options. Therefore, the identification of  VRSA is necessary to improve the management of antibiotic therapy, infection control and to prevent the occurrence of wider resistance. This retrospective descriptive study used secondary data of antibiotic susceptibility test on Staphylococcus aureus identified using VITEK 2 and patient medical records from January 2015 to December 2016. The data is processed using SPSS. Of 387 Staphylococcus aureus isolates obtained, 45 (11%) were VRSA. The most common VRSA isolates were male patients (57.8%) with the mean age being 41-60 years old (35.6%). The treatment ward with the most VRSA isolates were from surgical ward (20%), and internal medicine ward (15.6%) whereas the  VRSA isolates from outpatient record were from surgical  department (8.9%), dermatology department and ENT department (2.2%). The most common specimens were blood (28.9%), pus (26.7%), and sputum (20.0%). The highest prevalence of VRSA was found in the use of ≥ 3 invasive medical devices and from clinical outcome was found 11 (24.4%) of the patients died. Linezolid, Quinupristin / Dalfopristin, Tigecycline, and Nitrofurantoin as a therapeutic option have > 90% sensitivity. Identification of  VRSA in Dr. Wahidin Sudirohusodo Hospital Makassar emphasized the need for  immediate action by infection control division, especially in related units to prevent the transmission.
PERBANDINGAN ANTARA KADAR KALIUM SERUM DENGAN ATAU TANPA TERAPI INSULIN PADA PASIEN DIABETES MELLITUS TIPE 2 Andi Syamsudduha; S. V Sembiring; R DN Pakasi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.972

Abstract

Diabetic patients can suffer from hipokalemia because of prolonged osmotic diuretic and management diabetic mellitus with insulinif does not associate with kalium intake. The aimed of this study was to evaluate kalium serum level on type 2 DM, and comparisonof kalium serum level on type 2 DM patients with or without insulin therapy. A cross-sectional study was done on 33 patiens type 2diabetic mellitus that admission on Stella Maris Hospital who examined kalium serum level. The data were colected from Medical Recordperiode January to December 2007. Data were analyzed with Fisher Exact test using SPSS versi 11.5. From 33 samples found normalkalium level 9 samples (27.3%) and decreased 6 samples (18.2%) and without insulin therapy found decresed 3 samples (9.1%) andnormal Kalium level 15 samples (45.5%). Comparison of Kalium serum level of type 2 DM patiens with or without insulin therapydid not statistically significant with P = 0.135. Kalium serum level of type 2 DM patiens with or without insulin therapy commonly inreference value.
PERBANDINGAN CYSTATIN C DENGAN PARAMETER UJI FUNGSI GINJAL LAINNYA Pusparini .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 1 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i1.919

Abstract

The Gold standard for the evaluation of the glomerular filtration rate (GFR) is inulin clearance, but in widespread use is prevented by several technical difficulties. The most commonly used marker for GFR is serum creatinine alone or in conjunction with 24 hoururine collection for determination of creatinine clearance, but these marker have several limitation include following: influence of age,sex, muscle mass on endogenous creatinine production, dietary intake and the difficulties of 24 hour urine collection. Fifty six patientwith chronic renal failure and 53 control had analyze for serum creatinin, creatinine clearance and serum cystatin C. The chronic renalfailure patient aged range from (64 + 14.54) year and the control group aged range from (62.5+ 17.5) year. The proposed of this studywas to compare cystatin C with another parameter for renal function test. The result showed that in control group serum creatinineand creatinine clearance had influence with age, sex and body mass index, but serum cystatin C was not. The normal value of cystatinC was (0.85 + 0.13) mg/dL In chronic renal failure group there were significant correlation between level of cystatin C with creatininclearance (p = 0.000, r = 0.69). The level of cystatin C increase higher than serum creatinine in patient with low clearance creatinine.In control group we were determined low creatinine clearance in patient with normal serum creatinine and cystatin C.
ANALYSIS OF PLATELET COUNT ON FIBROSIS DEGREE IN CHRONIC HEPATITIS B PATIENTS Hairul Anwar; Mutmainnah Mutmainnah; Ibrahim Abdul Samad
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 2 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i2.1318

Abstract

Chronic hepatitis B is an infectious liver disease caused by hepatitis B virus that persist for more than 6 months. Fibrosis is a result of fibrogenesis which is the formation of connective tissue (scarring) caused by liver tissue damage. Liver damage will affect the production of thrombopoetin causing disturbances in the balance between destruction and production of platelet resulting in decreased platelet counts. This study was a retrospective cross-sectional study by taking the data from medical records of chronic hepatitis B patients who were tested for complete blood count and fibroScan at the Dr.Wahidin Sudirohusodo Hospital Makassar from January 2014 to July 2016. The result showed a total of 323 chronic B hepatitis patients, 99 with severe fibrosis, 84 with moderate fibrosis and 140 with mild fibrosis. The Spearman correlation test showed a significant correlation between the platelet count and the degree of fibrosis (p <0.001) and showed a positive correlation between both of them with a very strong correlation (r = 0.802). The Kruskal-Wallis test showed a significant difference between platelet count and the degree of fibrosis (p<0.001). The conclusion is that a decreased platelet count is a sign of an increase in the degree of fibrosis in chronic hepatitis B patients. It is suggested to perform another study with larger samples based on the degree of fibrosis. 
PENEMUAN (DETEKSI) ANTIBODI UNTUK ANTIGEN TUBERKULOSIS MENGGUNAKAN METODE IMUNOKROMATOGRAFI DI PENDERITA TUBERKULOSIS PARU Kadek Mulyantari; Aryati .; M.Y. Probohoesodo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 14, No 3 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v14i3.938

Abstract

The gold standard for TB still has some drawbacks, such as a long duration for culture examination and the rolated facilities are notalways available in all laboratories. One of methods in diagnosing tuberculosis infection is by immunochromatography (ICT). MYCOTECTB xp (recombinant) is one of serologic tests using immunochromatography principle. MYCOTEC TB xp uses recombinant antigens 38kDa, 16 kDa, 6 kDa and Early Secreted Antigen Target (ESAT-6). This method is expected so far diagnose TB in a short time and has ahigh accuracy. Evaluating the immunochromatography method in detecting antibody by tuberculosis antigen in lung TB patients as willthose with nonTB lung disease (lung tumor, bronchial asthma, pneumonia, chronic obstructive lungdisease). Serum samples of 30 TBpatients in BP4/Karang Tembok Hospital Surabaya and 30 non TB patients in the Dr. Soetomo Hospital Surabaya. Detection of antibodyto tuberculosis antigen was done with MYCOTEC TB xp. In this study found is prond 30 TB patients using MYCOTEC TB xp was positivein 23 samples and negative in 7 samples. From the 30 nonTB patients MYCOTEC TB xp was positive in 4 samples and negative in 26samples. It can be uncloaded so far that the diagnostic sensitivity of MYCOTEC TB xp was 76.7% (23/30) and diagnostic specificity was86.7% (26/30). MYCOTEC TB xp has an intermediate diagnostic sensitivity of 76.7% and a high diagnostic specificity of 86.7%.
ANALISIS KADAR ALBUMIN SERUM DENGAN RASIO DE RITIS PADA PENDERITA HEPATITIS B AT. Lopa; B. Rusli; M. Arif; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 2 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i2.884

Abstract

Hepatitis is a inflammation process which may present in an acute or chronic phase. A decrease of serum albumin level may befound in liver disease. Ratio de Ritis in mild hepatitis is < 1 while in alcoholic hepatitis is >1. To analyze serum albumin level withratio de Ritis in hepatitis B patients. A cross sectional study was carried out comprising 46 subjects from October 2005 to August 2006measuring albumin level SGOT and SGPT levels using Lyasis autoanalyzer. Thirty eight males and eight females, the level of serumalbumin decreased with an average of albumin level 2.98 gr/dL (p<0.05). Twenty two subjects showed a ratio de Ritis ≤ 1 with theaverage albumin level 3.00 gr/dL and 24 subjects were included in group ratio de Ritis > 1 with average albumin level 2.96 gr/dL(p value = 0.658). There was a decrease of serum albumin level in hepatitis B patient but no significant difference between decrease ofserum albumin level with ratio of de Ritis ≤ 1 and ratio of de Ritis >1.
UJI GLUKOSA DARAH ANTARA METODE HEKSOKINASE DENGAN GLUKOSA OKSIDASE DAN GLUKOSA DEHIDROGENASE DI DIABETES MELITUS Baharuddin Baharuddin; Asvin Nurulita; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1102

Abstract

Diabetes mellitus is a group of metabolic diseases characterized by chronic hyperglycemia that occurs because of abnormalities insulinsecretion, insulin or both. Diabetes mellitus diagnosis is established on the basis of examination of blood glucose levels. The recommendedblood glucose test is enzymatic method by hexokinase using venous blood plasma, while for monitoring the outcomes of treatment: bloodglucose test can be done by rapid test using capillary blood. The purpose of this study was to know the test result of blood glucose betweenhexokinase method (ABX Pentra 400), glucose oxidase (StatStrip Xpress, Super Glucocard II) and glucose dehydrogenase methods(Accu-Chek Performa) by comparing them Cross sectional study was conducted on 50 samples of patient hospitalized at Dr. WahidinSudirohusodo Hospital Makassar. The blood glucose test were performed at the Departement of Clinical Pathology from June up to July2012. Each plasma sample was tested using ABX Pentra 400, StatStrip Xpress, Super Glucocard II and Accu-Chek Performa. The datawere analyzed using Kolmogorov Smirnov and Mann Whitney test. Based from this study was showed that there was no difference in bloodthe level using a related (glucose) oxidase (StatStrip Xpress, Super Glucocard II) and its dehydrogenase method (Accu-Chek Performa)compared with hexokinase method (ABX Pentra 400) with p value > 0.05. The result obtained in this study showed that StatStrip Xpress,Super Glucocard II and Accu-Chek Performa can be used as a rapid test for monitoring blood glucose in hospitalized patients.
RELATIONSHIP BETWEEN D-DIMER LEVEL AND CLINICAL SEVERITY OF SEPSIS Yessy Puspitasari; Aryati Aryati; Arifoel Hajat; Bambang Pujo Semedi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1196

Abstract

D-dimer merupakan tolok ukur laboratorium yang menunjukkan derajat keparahan pada sepsis. Selama tahapan sepsis terjadiaktivasi prokoagulan yang tidak diimbangi aktivitas antikoagulan (depresi protein C dan meningkatnya pelepasan Plasminogen activatorinhibitor) sehingga dapat meningkatkan hasilan fibrin polimer. Fibrin polimer yang telah mengalami cross-linked akan difibrinolisis olehplasmin membentuk formasi D-dimer. Tujuan penelitian untuk menganalisis hubungan D-dimer dengan derajat keparahan klinis darisepsis. Metode penelitian bersifat potong lintang observasional. Sampel darah sitrat dari 52 pasien sepsis yang dirawat di IRD, ICU, ROI,Ruang penyakit dalam RSUD. Dr. Soetomo Surabaya, dikumpulkan selama Februari 2016–Juni 2016. Kadar D-dimer diukur denganmetode ELFA (Enzyme Linked Fluorescent Assay). Proses dan tafsiran data menggunakan analisis deskriptif, One sample Kolmogorovsmirnovdan uji Pearson digunakan untuk menganalisis kenasaban. Didapatkan rerata kadar D-dimer 3879,46±2800,29 ng/mL.D-dimer pada non-survivors sepsis menurut skor APACHE II dan SOFA lebih tinggi daripada survivors sepsis. Terdapat kenasabanpositif yang bermakna antara kadar D-dimer dengan skor APACHE II dan skor SOFA r=0,513 dan r=0,580 (p=0,01). Berdasarkantelitian ini dapat disimpulkan D-dimer memiliki kenasaban dengan derajat keparahan klinis dari sepsis, semakin tinggi nilai D-dimermenunjukkan keparahan sepsis.
KADAR HEMOGLOBIN RETIKULOSIT DI ANEMIA DAN NONANEMIA AKIBAT DEFISIENSI BESI ABSOLUT DI GAGAL GINJAL TERMINAL TERKAIT HEMODIALISIS (Reticulocyte Hemoglobin Level of Absolute Iron Deficiency Anemia and NonAbsolute Iron Deficiency Anemia In End State Renal Disease Undergoing Maintenance Hemodialysis) Amelia Rachmiwatie; Noormartany Noormartany; Rubin Surachno Gondodiputro; Delita Prihatni
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 1 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i1.1256

Abstract

Absolute Iron Deficiency Anemia (IDA) is one of the complications in the End State Renal Disease (ESRD) patients undergoingmaintenance hemodalysis, with an incidence of 76.4%. If this condition is not properly addressed, it can lead to impaired cardiac functionand increased mortality. The incidence of this case is between 30−45%. The determination of the iron status in ESRD patients wqs setby the Perhimpunan Nefrologi Indonesia 2011 using serum ferritin and transferrin saturation, but they do not provide a meaningfulchange in the value of the initial state of the iron deficiency. This condition should be examined with other parameters combination aswell as those influenced by diurnal variation and infection-inflammation condition. Reticulocyte hemoglobin (Ret-He) parameter canbe used as a marker of bone marrow iron availability because these reticulocytes are young erythrocytes released from the bone marrowinto the circulation. These conditions were circulated only within a short time about 1-2 days before becoming mature erythrocytes. Theaim of this study was to determine the Ret-He level diferentiation between absolute IDA and non absolute IDA states in ESRD patientswhom underwent maintenance hemodialysis. This research was conducted in the Laboratory of Clinical Pathology-RSHS-Bandung fromSeptember 2012 to June 2013. The study design was cross-sectional. All subjects were ESRD patients undergoing hemodialysis maintenancefor at least three (3) months and consisted of absolute IDA and non-absolute IDA based on the results of the ferritin and transferrinsaturation calculations according to the criteria of Pernefri 2011 Reticulocyte hemoglobin levels were checked using a fluorescenceflowcitometry principle in the automated hematology analyzer. In this study it was found that the Ret-He mean in the absolute IDA was26.1 pg/cell and 35.9 pg/cell in non absolute IDA. Statistical analysis was performed using Independent T-test. A total of 61 undergoingmaintenance hemodialysis subjects participating in this study comprised patients with absolute IDA and non-absolute IDA who met theinclusion and exclusion criteria. It can be concluded that the Ret-He level in statistical analysis showed absolute IDA which was meaningfullower than nonabsolute IDA in ESRD patients undergoing maintenance hemodialysis (p<0.001).
GAMBARAN MIKROBIOLOGI ISPA (INFEKSI SALURAN PERNAPASAN ATAS) DI SEKELOMPOK JAMAAH HAJI SURABAYA TAHUN 2004 Prihatini Prihatini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 1 (2005)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i1.834

Abstract

Upper respiratory tract infection usually has been presence on hajj pilgrims after they spent at the holy Mecca. They are known by long duration cough until they were come home. The pilgrims have been given health education how to live in Mecca and Medina before they go to Saudi Arabia and had meningitis vaccination as well. The purpose of this study is to know what the cause of the upper respiratory tract infection. If the pathogens have been found, before departure the infected pilgrims have been given antibiotics to prevent the pilgrimage ceremony to be disturbed.. Regarding the infection problems this study will be done, to give information whether the pathogenic that cause URI is from Indonesia or Saudi Arabia. About 118 people partially from Surabaya’s pilgrims were divided into 1st and 2nd groups (53 and 65 persons). Each group have been examined their pharyngeal swab before the departure to Mecca and after arrival in Surabaya. The samples were kept in transport media, than sent to the Clinical Pathologic Laboratory at Dr Soetomo Hospital. The swab samples were isolated and identificated after the cultivation in the incubator at the laboratory. From the118 pilgrims, only 95 persons completed the laboratory examination before the departure to Mekah and after they arrived in Surabaya. It is found before departure 5 person (5%) contaminated by pathogenic microorganism, four from K. pneumoniae and one A betahemolytic Streptococcus group. After their arrival about 97% have normal flora, but two of them contaminated by Gamma Streptococcus regarding to these results it is concluded that URI may cause by the environment, difference of weather or viral infection origin Because in the town at Saudi Arabia the pilgrim lived together with other peoples which came from various countries of the world.

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