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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,183 Documents
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DIAGNOSTIC TEST ON THE FOURTH GENERATION HUMAN IMMUNODEFICIENCY VIRUS IN HIV SUSPECTS Sofitri Sofitri; Ellyza Nasrul; Almurdi Almurdi; Efrida Efrida
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1142

Abstract

Diagnosis dini infeksi Human Immunodeficiency Virus (HIV) dapat mengurangi kebahayaan transmisi. Infeksi akut dapat ditetapkanberdasarkan pemeriksaan antigen atau asam ribonukleat (RNA/proviral DNA) HIV. Enzyme Immunoassay (EIA) generasi keempatadalah immunoassay yang dapat mendeteksi antigen p24 dan antibodi HIV. Tujuan penelitian adalah mengetahui nilai diagnostik ujiHIV generasi keempat di terduga HIV. Penelitian ini merupakan uji diagnostik dengan desain potong lintang. Sampel penelitian adalahsemua pasien terduga HIV yang datang ke poliklinik Volunters Counselling and Testing (VCT) RSUP Dr. M. Djamil Padang masa waktuMaret 2015–Maret 2016. Penelitian ini dilakukan untuk menilai ketepatan diagnostik (kepekaan, kekhasan, nilai peramalan positif,nilai peramalan negatif) uji HIV generasi keempat menggunakan Enzyme Linked Fluorescent Assay (ELFA) terhadap deteksi RNA HIVmenggunakan Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) serta dianalisis menggunakan tabel 2×2. Subjek penelitiansebanyak 70 orang terduga HIV terdiri dari 46 laki-laki (65,7%) dan 24 perempuan (34,3%) dengan rerata umur 27,7 tahun. TransmisiHIV terbanyak adalah perilaku heterokseksual (45,7%). Nilai diagnostik uji HIV generasi keempat terhadap RNA HIV didapatkankepekaan 95%, kekhasan 96%, nilai peramalan positif 97% dan nilai peramalan negatif 92%.
NILAI DIAGNOSTIK MALARIA ANTIGEN CASSETTE PENYAKIT MALARIA Binawati .; Prihatini .; M.Y Probohoesodo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.1031

Abstract

Malaria is an endemic disease in many countries. In 103 endemic countries with around 2.5 billion population, 1−3 million death cases were reported every year. Clinical criteria and blood smear established the diagnosis of malaria. ICT (imuno chromatographytest) is needed in peripheral areas where there are no experienced laboratory technicians. The procedure is simple, practical, easy, aswell as quicker than the conventional method, and no experienced technicians are needed this ICT advantages. The aim of this study isto know the diagnostic value of Malaria Antigen Cassette with microscopic examination as the gold standard examination of malaria.This research used observational cross-sectional method This study was done in Nusa Tenggara Barat during May−June 2008. Bloodsamples were taken by finger prick in patients with Malaria symptoms: fever, chill and sweating, followed by examining the blood smearby Malaria Antigen Cassette from Focus Diagnostic. The diagnostic value was then evaluated by calculating the sensitivity, specificity,positive predictive value as well as the negative predictive value. The diagnostic value of Malaria Antigen Cassette in patients withmalaria falciparum was found as follows: sensitivity 95.2%, specificity 100%, positive predictive value 100%, and negative predictivevalue 97.6%. The diagnostic value of Malaria Antigen Cassette in patients other types than malaria falciparum was as follows: sensitivity94.8%, specificity 100%, positive predictive value 100%, and negative predictive value 95.3%. The diagnostic value of Malaria AntigenCassette was very high in diagnosing malaria falciparum compared to other types than malaria falciparum.
ANALYSIS OF SOLUBLE FIBRIN MONOMER AS DIAGNOSTIC MARKER FOR ACUTE MYOCARDIAL INFARCTION AND ITS CORRELATION WITH CARDIAC TROPONIN I Maimun Zulhaidah Arthamin; Lydiana Parmadi; Dwi Priyadi Djatmiko; Elvin Richela Lawanto
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 3 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i3.1505

Abstract

Background. The diagnosis of non-ST-segment elevation myocardial infarction (NSTEMI) is required early and accurate to avoid missing diagnosis and improve the rule out of AMI patients. There is a relationship between AMI and the state of hypercoagulation and/or thrombosis process. sFM is a protrombotic marker that is found to be associated with early AMI incidence compared to cTnI that increases after mionecrosis. The aim of this study is to determine that sFM can be used as biomarker for AMI and the correlation between sFM and cTnI.Methods. A cross-sectional analytic observational study was conducted among 23 AMI patients and 27 healthy controls. AMI were established using clinical, ECG and laboratory findings. sFM levels were measured with Stago Compact Max analyzer. Statistical analysis was performed using the Spearman’s correlation coefficient, ROC curve analysis, and 2x2 contingency table.Results. A significant correlation were found between the sFM and the cTnI (r=0.422, p<0.05). With a sFM cutoff level of 2.56 µg/mL, AMI could be diagnosed with sensitivity and specificity of 82.6% and 40.7%, respectively (AUC=0,638).Discussion. sFM is a new biomarker for systemic thrombus events, both cardiac and non-cardiac.Conclusions and Suggestions. sFM can be considered as an parameter of AMI. Similar studies with cohort method involving large number may be needed in the future study. 
LACTATE DEHYDROGENASE (LDH) SELAMA PENYIMPANAN Teguh Triyono; Umi Solekhah Intansari; Caesar Haryo Bimoseno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 3 (2013)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i3.416

Abstract

During storage, erythrocytes suffered from biomechanical alterations called the “storage lesion”, which may caused hemolysis. The hemolysis released LDH into the plasma. The LDH that was released during hemolysis made it an adequate instrument to assess the quality of in vitro blood products. The aims of this study were to analyse the alteration of LDH level at day 1, 3, 7, 14, and 28 in the WB and PRC, to analyse the correlation between LDH level with storage duration, and also to analyse enhancement differences of LDH level between WB and PRC.This research was an observational study with a cross-sectional design. As the samples there were 11 bags of WB and 10 bags of PRC. Blood products were kept in bloodbank with the temperature range of 2–6° C. The LDH level was measured with the Beckman Chemistry Analyzer. There were statistically significant alterations of LDH level started from day 7 of storage in both blood products (p<0.05). The significant strong correlation between LDH level with the storage duration were found r=0.772; r=0.835 (p<0.05) in WB and PRC respectively. The enhancement differences were found to be higher and significant in the PRC than in the WB started from day 7 of storage (p<0.05). As conclusion, LDH in WB and PRC were signifantly increased during storage, and correlate with storage duration.
NEUTROPHIL-LYMPHOCYTE COUNT RATIO IN BACTERIAL SEPSIS Danny Luhulima; Marwito Marwito; Eva O
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1204

Abstract

Sepsis akibat infeksi bakteri merupakan masalah kegawatdaruratan medik yang serius sehingga memerlukan penanganan cepat dantepat. Saat ini C-RP (C- reactive protein) dan PCT (procalcitonin) sering digunakan sebagai petanda sepsis bakterial. Sepsis adalah infeksiyang disertai inflamasi sistemik. Respons fisiologis terhadap inflamasi sistemik adalah peningkatan jumlah neutrofil dan penurunanjumlah limfosit, sehingga gabungan perbandingan neutrofil dan limfosit Neutrophil Lymphocyte Count Ratio (NLCR)) dapat digunakansebagai petanda sepsis. Penelitian ini bertujuan untuk mengetahui kepekaan dan kekhasana dari uji NLCR di pasien sepsis akibatinfeksi bakteri. Terdapat 70 pasien SIRS dengan rentang usia 14–70 tahun di RS Mitra Keluarga Bekasi Timur dan RS FK - UKI Jakartamasa waktu bulan Juli–September 2015. Penelitian ini merupakan studi observasional komparatif dan potong lintang. Hasil penelitianmenunjukkan uji NLCR terhadap sepsis bakterial berdasarkan kurva ROC memiliki kepekaan 97,8% dan kekhasan 84,0% pada cutoff ≥6,4 (AUC: 0,94, nilai p<0,05). Neutrophil lymphocyte count ratio dapat diandalkan sebagai petanda sepsis bakterial dengan ujikepekaan dan kekhasan yang baik.
ASSOCIATION BETWEEN SPESIFIC ENOLASE SERUM LEVELS AND OUTCOME ACUTE ISCHEMIC STROKE ONSET 1-MONTH Yuri Haiga; Darwin Amir; Yuliarni Syafrita
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1377

Abstract

Pendahuluan: Stroke adalah masalah kesehatan global, karena penyebab kematian kedua dan kecacatan utama pada hampir seluruh negara di dunia. Penyakit ini memiliki dampak yang besar terhadap penderita, lingkungan sosial dan beban ekonomi. Sehingga diperlukan penanda spesifik yang berperan dalam menegakkan diagnosis, penentuan faktor risiko, serta tingkat keparahan stroke iskemik. Salah satu penanda yang diteliti adalah kadar NSE serum, yang diperkirakan dapat menggambarkan beratnya kerusakan otak pada pasien stroke.Metode: Penelitian ini menggunakan metode Cross sectional study pada 77 pasien stroke iskemik , dilaksanakan mulai Juli 2016 sampai Agustus 2017, di ruang rawat inap di Bagian Penyakit Saraf RS. DR. M. Djamil Padang. Setiap pasien dilakukan pemeriksaan kadar NSE serum dan  penilaian skor mRS pada bulan-1 setelah onset stroke iskemik. Dan dilakukan uji Spearmen untuk menilai korelasi antara dua variabel. Nilai p <0.05 dianggap signifikan secara statistikHasil: Berdasarkan jenis kelamin didapatkan laki-laki 42 orang (54.54%).  Median  usia 58.21 (16-88). Median kadar NSE  5.94 ( 2.77-36.75) µg/L. Skor  mRS onset bulan-1 dengan  median 3 (1-6). Terdapat hubungan antara kadar serum NSE dengan fungsi luaran stroke iskemik onset bulan-1 (r = 0.286, p value= 0.012, R2= +8.2%)Diskusi: Terdapat hubungan antara kadar serum NSE dengan fungsi luaran stroke iskemik onset bulan-1.Kesimpulan: Terdapat hubungan antara kadar serum NSE dengan fungsi luaran stroke iskemik onset bulan-1.
, DALAM KEADAAN (STATUS) IMUNOLOGIS DAN KLINIS PENGOBATAN ANTIRETROVIRAL PENDERITA HIV/AIDS CD38 LIMFOSIT CD8 + , TAMPANG (PROFIL) CD4 + Ira Puspitawati; Umi S. Intansari
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.994

Abstract

ABSTRACTThe relationship between immune activation and pathogenesis of Human Immunodeficiency Virus (HIV) infection is unproven. It has been hypothesized that state that HIV-induced activation enhances the magnitude of HIV replication other study shown that viralreplication impaired immune activation. One of the best immune activation marker is CD38 expression on CD8because it has thestrongest significant prognostic markers. It was previously shown that increased CD8+ T-cell activation has predictive value for diseaseprogression but the relation is still controversial. One hypothesis state that HIV-induced activation enhances the magnitude of HIVreplication, other said that viral replication impaired immune activation. To know the profile of immunology and clinical state of HIV/AIDS patients by determining the CD38 molecule expression on CD8+ profiles and clinical state.Crosssectonal, observasional study was done. Twenty nine HIV/AIDS patients who routinely had medical check up and having routine +cells as an activation marker, CD4+antiretroviral therapy at Sardjito hospital and 8 healthy people as normal controls were involved in this study. The count of CD4 +cells counts had significantnegative correlation with WHO stage (p = 0.012). Expression of CD38 molecule on CD8absolute counts and expression of CD38 molecule on CD8 cells were measured using flowcytometry. The CD4+cells of HIV/AIDS patients were highercompared to normal controls, 209.29 ± 76.56, 109.61 + 32.29 respectively (p < 0.05). That expression was not correlated with CD4 + +counts and CD4incrementafter therapy was statistically significant (p = 0.003). The CD4+increment. It might be caused by the time of measurement was not at the begining of diseases. The CD4 + count was negatively correlated with the WHO stage. Expression ofCD38 molecule on CD8+ cells of HIV/AIDS patients were significantly higher compared to normal controls but it wasn’t correlated withthe CD4++ number and CD4+increment after therapy.
AUDIT SYSTEM FOR USAGE OF BLOOD PRODUCT IN SANGLAH GENERAL HOSPITAL DENPASAR BALI Kadek Mulyantari; Teguh Triyono; Usi Sukorini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 3 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i3.1382

Abstract

Blood transfusion is one of very important therapy components. One indicator of the blood services quality in Sanglah  General Hospital Denpasar Bali Indonesia is achieving the target of unused blood order ≤ 10% per month. However, these targets have not been achieved. The number of unused blood orders reached 6,719 bags per year (600 bags per month or 21.5%). Unused blood orders is mostly from Department of Internal Medicine (40.9%), followed by Department of Surgical (35.6%),  Department of Obstetrics and Gynecology (15.5%) , and Department of Pediatrics (8%). The high number of unused blood orders can causes several problems such as the disorder of blood supply, inefficiency of pre-transfusion testing and decreased quality of blood product. Considering the consequences of high unused blood order, it is necessary to conduct a research on audit system for usage of blood product in Sanglah Hospital.The aim of study are to analyze the influence of audit system to reduce unused blood order and to decrease the number of C/T ratio. The study design was a pre and post test intervention study. The population in this study were all forming of blood requests in Sanglah Hospital. The sample is blood request form Department of Internal Medicine of the last two months. Intervention in this study was the development and dissemination of guidelines for usage of blood products, followed by concurrent audit and prospective audit of the sample.The study show there are decline of unused blood orders and C/T ratio before and after implementation of the audit system for usage of blood products. Reduction of unused blood order in Sanglah Hospital is 5.4%, and in the Department of Internal Medicine is 5.2%. The causes of unused blood orders were patient died, overestimation of blood order, transfusion delays because waiting for the schedule of hemodialysis, the patient has a fever, blood product has expired, and the occurrence of administration errors. Reduction of C/T ratio in Sanglah Hospital is 0,094 and in the Department of Internal Medicine is 0,072. Audit system for usage of blood products can reduce unused blood orders and decrease C/T ratio.
PHYLLANTHUS NIRURI L TERHADAP IMUNITAS SELULER TIKUS Ima Arum L; Purwanto AP; Henna Rya
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 1 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i1.358

Abstract

Typhoid fever is a problem in the developing country. Phyllanthus niruri L (meniran) is a traditional plant that can be used as an immunomodulator on cellular or humeral immunity. By giving Phyllanthus niruri L extract to patient who suffers typhoid fever can increase cellular immunity responses in intracellular bacteria infection (S.typhimurium).This research was an experimental study using the post test-only control group design to proof of the effects of Phyllanthus niruri L on cellular immunity balb/c infected by salmonella typhimurium. The samples were taken from thirty male Balb/c mice which were divided into five groups including P1 (infected by S.typhimurium and PnL extract 125 g), P2 (infected by S.typhimurium and PnL extract 250 g), P3 (infected by S.typhimurium and PnL extract 500 g), K1 (infected by S.typhimurium) and K2 (healthy mice). In this study was applied parametric test of one way ANOVA and post hoc test Bonfferoni. It was known that phyllanthus niruri L increased the number of activated lymphocytes and phagocyte ability of macrophage (p=0.003 and p=0.001, respectively). By Bonfferoni post hoc test resulted that no significantly difference between the treated groups (increased of dose). There were no significant difference between the number of leukocyte, the difference count of lymphocyte and the nitrite oxide produced among the treatment groups and controls. Phyllanthus niruri L dose 250 μg increased the activated lymphocyte and index phagocytes. To find out the effects of Phyllanthus niruri L compare to antibiotic and Phyllanthus niruri L combination, continuing/advancing studies are needed
DETERMINATION OF REACTIVE HBsAg CUT-OFF THAT NEED CONFIRMATORY TEST Sherly Purnamawaty; Irda Handayani; Asvin Nurulita; Uleng Bahrun
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 3 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i3.1335

Abstract

Hepatitis B surface antigen (HBsAg) is the earliest and most important serological marker for the diagnosis of HBV infection. The availability of new methods with a high sensitivity to detect HBsAg results in the increase of false reactive results so that a confirmatory test is needed,but this will increase the total test cost. A reactive cut-off value for a confirmatory test is needed to make the use of this test more efficient. This study was a cross-sectional. All the specimens with HBsAg >0.17 Cut-Off Index (COI) were confirmed with HBsAg confirmatory test. HBsAg test used a sandwich ELFA method while HBsAg confirmatory test used an antibody neutralization method. Analysis of the ROC curve obtained HBsAg cut-off value that need confirmatory test. Total samples were 80 with 51 (63.8%) confirmed reactive and 29 (36.2%) non-reactive. There was a statistically significant difference between HBsAg that confirmed reactive (median 2.76 COI) and non-reactive (median 0.32 COI) (p<0.001). ROC curve showed an AUC of 0.805 which meant a good diagnostic performance for HBsAg test based on a confirmatory test. The specificity of 89.66% and sensitivity 64.71% were obtained from the cut-off 1.08 COI and considered the best cut-off. Some possible causes of false reactive results were Hepatitis B vaccine, G-CSF therapy and limitation of the HBsAg methods. HBsAg cut-off with ELFA method that need HBsAg confirmatory test was <1.08 COI. The researchers suggests further studies with different sampling methods so a better data distribution can be obtained.

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