cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Dr. dr. Puspa Wardhani, SpPK
Contact Email
admin@indonesianjournalofclinicalpathology.org
Phone
+6285733220600
Journal Mail Official
majalah.jicp@yahoo.com
Editorial Address
Laboratorium Patologi Klinik RSUD Dr. Soetomo Jl. Mayjend. Prof. Dr. Moestopo 6-8 Surabaya
Location
Kota adm. jakarta selatan,
Dki jakarta
INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 11 Documents
 1   2 
Search results for , issue "Vol 18, No 3 (2012)" : 11 Documents clear
COLD AGGLUTININ PADA PENDERITA COMMUNITY ACQUIRED PNEUMONIA Johanis Johanis; Juli Soemarsono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.379

Abstract

Cold agglutinins at below physiologic body temperature can cause spontaneous agglutinations of erythrocytes. Cold agglutinins result from a particular antibodies activation on erythrocytes associated with a primary disease, including infection. The generation of antibody activates complement resulting in hemolysis. A 63-year-old man suffered from shortness of breath accompanied with productive cough, fever, right chest pain, loss of appetite, nausea, and occasionally vomiting. Physical examination showed an increase of pulse rate, respiration rate, and body temperature. Lung examination showed right intercostals retraction and rales in both lungs, but no abnormality detected in other organs. Chest X-ray showed pneumonia. EDTA whole blood showed spontaneous agglutinations at room temperature, however this did not occur by maintaining temperature at 37° C. Different complete blood count results were shown between agglutinated blood and absent of agglutination blood samples. As anti-I, anti-i, and/or anti-H was suspected, agglutinations for anti-A and anti-AB occurred by using ABO forward grouping test, whereas reverse grouping showed agglutinations for A, B, and O cells. Protein electrophoresis showed increase of alpha- 1 and gamma globulin; decrease of renal function; slightly increase of indirect bilirubin; and suspected Extended Spectrum Beta-Lactamase (ESBL) Klebsiella pneumoniae. The diagnosis of this case was community acquired pneumonia and suspected ESBL. Cold agglutinins affected CBC evaluations mostly shown in the erithrocyte index, nevertheless this could prevented by maintaining at physiologic body temperature. Infection could induce activation of cold agglutinins.
MYCOBACTERIUM TUBERCULOSIS DAN PCR Yuyun Widaningsih; Ismawati Amin; Nurhayana Sennang; Uleng Bahrun; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.756

Abstract

TB’s coinfection in HIV and AIDS is on the increase together with the high mortality rate. The disseminated TB infection (in blood) of HIV and AIDS patients in Africa (2005) is about 10−25%. The aim of this study was to identify M.tb disseminated infection in HIV and AIDS patients. In this study was done in six HIV and AIDS patients suffering from suspected TB co infection. The sputum was tested using Fast Acid Bacilli and culture (using LJ and MGIT media) and DST. The identification of M.tb in blood used PCR with IS6110 primer (123 bp). The study was done at Dr. Wahidin Sudirohusodo Hospital, Labuang Baji Hospital, NEHCRI and at the Biomolecular Laboratory of the Medical Faculty, Hasanuddin University. From the six patients, four of them had CD4<50 sel/μL, two patients had FAB positive, three patients had positive LJ culture on day 41–47, three patients had positive MGIT culture on day 9–47 and four patients had positive PCR. In this study there was one case of negative FAB but LJ, MGIT and PCR were positive. There were two cases with positive FAB, culture and PCR, one case with negative FAB and culture but positive PCR. The M.tb disseminated infection was 66.7% in six HIV and AIDS patients.
CRYPTOSPORIDIOSIS PARU DI PENDERITA TBC R. Heru Prasetyo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.375

Abstract

The pulmonary cryptosporidiosis cases had been reported for immunocompromised persons, most all of whom were secunder infected with HIV and AIDS patients. Tuberculosis is a chronic respiratory disease and tending to cause a weakened immune system (immunocompromised). However, pulmonary cryptosporidiosis has not been previously reported as secunder infection in tuberculosis patients. The objective of this study was to know the prevalence of pulmonary cryptosporidiosis determination in tuberculosis patients. This research was carried out by a cross sectional study utilitzing waste sputum samples from tuberculosis patients. The detection of Cryptosporidium oocyst used modified version acid fast stain of Ziehl Neelsen technique. Three sputum samples among 44 sputum samples (6.8%) had Cryptosporidium oocyst positive. These findings suggest that there was a potential for respiratory transmission of cryptosporidiosis. Although the prevalence of pulmonary cryptosporidiosis in tuberculosis patients are low, the researcher suggest that the possibility of pulmonary cryptosporidiosis as a secondary infection in tuberculosis patients existed, and there for a laboratory examination of pulmonary cryptosporidiosis becoming routinely laboratory for tuberculosis patients.
PEMERIKSAAN PROTHROMBIN TIME DAN ACTIVATED PARTIAL THROMBOPLASTIN TIME DENGAN HUMACLOT VA SERTA SYSMEX CA 500 Misnah Misnah; Agus Alim Abdullah; Mansyur Arif; Burhanuddin Bahar
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.382

Abstract

Prothrombin Time (PT) and an activated Partial Thromboplastin Time (aPTT) are routine coagulation tests used for pre-operative screening. The analytical step as one of the laboratory test’s stage that plays the role in the determination of the test is influenced by several factors, one of them is choosing its proper devices. The aim of this study was to know the correlation of the PT and aPTT test’s result using Humaclot VA and Sysmex CA 500 devices. A cross sectional study has been done at the Clinical Pathology Laboratory of Wahidin Sudirohusodo Hospital Makassar started from May 2009 until June 2009. The data were analyzed with T and Pearson’s Correlation test. From the 50 samples were obtained the percentage of the corresponding frequency of the PT results between Humaclot VA and Sysmex CA 500 about 84%, whereas the frequency of the corresponding results aPPT between Humaclot VA and Sysmex CA 500 is 76%, the Pearson correlation test for PT=0.58, and aPTT=0.38. There were found the suitability of PT, aPTT of Humaclot VA with CA 500 and both tools have a positive correlation.
BAKTERI AEROB PATOGEN DAN UJI KEPEKAAN ANTIMIKROBA DI RUANGAN PERAWATAN PENYAKIT DALAM Fedelia Raya; Nurhayana Sennang; Suci Aprianti
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.374

Abstract

Pathogenic bacteria are the major causes of airborne infection at the hospital ward. Nosocomial infection can occur at the opened as well as at the closed room. Nosocomial infection influences the morbidity and mortality in the hospital and need an extra attention, because of the increased number of hospital patients, micro organism mutation and increased of bacteria resistance to antibiotics. The aim of this study was to quantify the number of aerobic bacteria, and to know the pathogenic bacteria identification and its determination on the susceptibility of the antimicrobial problems at the internal medicine ward. This research was carried by a cross sectional study, which performed by collecting air samples in eight internal medicine ward of Dr. Wahidin Sudirohusodo Hospital using Microbiology Air Sampler 100 (MAS 100). The bacterial identification and the antimicrobial susceptibility test (AST) were conducted at the Balai Besar Laboratorium Kesehatan (July to August 2009). In this study were found the numbers of bacteria colonies about 580–6040 CFU/m3. The pathogenic bacteria that identified were Acinetobacter calcoaceticus, Staphylococcus saprohpyticus, Enterobacter hafniae and Stomatococcus mucilaginosus that were sensitive to Amikasin, Gentamicyn, Azitromycin and Norfloxacyn but resistant to Ampicillin. The number of bacterial colonies exceeded the established number standard by Decree of the Indonesian Health Minister. The pathogenic bacteria showed the most sensitive result of AST were Acinetobacter calcoaceticus, Enterobacter hafniae, Stomatococcus mucilaginosus and Staphylococcus saprohpyticus.
ASOSIASI HLA-DRB1* DAN HLA-DQB1* DENGAN IGM-RF SERUM PADA ARTRITIS REUMATOID Joewono Soeroso; Ferdinandus Maria Judajana; Handono Kalim
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.373

Abstract

Rheumatoid arthritis (RA) is a chronic progressive polygenic autoimmune disease with a high socio-economic burden. Genetic polymorphisms expressed as a shared epitope (SE) at hypervariable regions of HLA-DRB1*01/*04/*10/*14 and linkage disequilibriums of HLA-DRB1*-HLA-DQB1* with some microorganisms, increase the risk of RA. Shared epitopes are also related with the increase of serum IgM-RF as well. A cross-sectional study on 48 RA patients diagnosed by ACR Criteria 1987 from 6 hospitals in Java and Bali was done to determine the associations of HLA-DRB1* and HLA-DQB1* alleles with serum IgM-RF levels. The presence of HLA-DRB1* and HLA-DQB1* alleles were assessed through PCR-SSO. Serum IgM-RF levels (IU/mL) were assessed through ELISA. Man-Whitney U tests were used to measure the associations. Value of p<0.05 is considered to be statistically significant. The results showed that mean ranks IgM-RF levels of subjects who are bearing HLA-DRB1*04 compared to subjects who are not bearing HLA-DRB1*04 were 34.10 IU/ml (n=10) vs 21.97 IU/ml (n=38), p=0.014. While, the mean ranks of IgM-RF levels of subjects who are bearing haplotype combination of HLA-DRB1*04-HLA-DQB1*03 vs subjects who are not bearing haplotype combination of HLA-DRB1*04- HLA-DQB1*03 were 32.89 IU/mL (n=9) vs 22.56 IU/mL (n=39) (p=0.046). It is concluded that there are association of HLA-DRB1*04, and haplotype combination of HLA-DRB1*04-HLA-DQB1*03 with increased levels of IgM-RF among Indonesians with RA.
KORELASI FUNGSI HATI TERHADAP DERAJAT PENYAKIT DEMAM BERDARAH DENGUE ANAK Ani Kartini; Mutmainnah Mutmainnah; Ibrahim Abdul Samad
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.378

Abstract

Dengue Haemorrhagic Fever (DHF) may result acute liver failure. Although these cases are rare but is more common in children, and early identification of these infections is important to reduce the morbidity as well as mortality. The aim of this study was to know the determinations and the correlation of liver functions with the grade of Dengue Haemorrhagic Fever in children. This study was conducted by cross sectional, using data from the medical records of DHF patients who had liver function tests (AST, ALT, PT, aPTT and albumin) in the Laboratory of Clinical Pathology Dr. Wahidin Sudirohusodo Hospital Makassar from the period of July 2008 to June 2010. AST, ALT and albumin were tested using Cobas Integra 400, PT and aPTT were tested using Humaclot VA, and the data were analyzed with Spearman correlation test. There were fifty-five samples: 26 (47.3%) were males and 29 (52.7%) were females. We found 15 (27.3%) grade I, 13 (23.6%) grade II, 16 (29.1%) grade III and 11 (20%) grade IV patients. In this study are found that AST and ALT increased and there is a tendency of extending aPTT value but there was no significant correlation with the grade of DHF. The mean of albumin was lower in grade IV, there was a significant correlation between albumin and the grade of DHF (r=–0.375, p<0.05). In conclusion, there was a significant correlation between albumin and the grade of DHF. It is suggested that the routine albumin examination in DHF patients should be carried on to know whether a prevention of complications is needed to reduce the possibility of mortality.
NILAI DIAGNOSTIK ANTIGEN TB DENGAN RAPID TEST DEVICE (TB AG) UNTUK TUBERKULOSIS PARU Sri Kartika Sari; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.381

Abstract

In Indonesia, the diagnosis of pulmonary tuberculosis relies primarily on an identification of acid-fast bacilli on sputum smears. However, microscopic device has several limitations. The sensitivity of microscopic examination is variable. The quality of smear microscopic results is heavily depend on the workload, and the skill of the technician’s reading the slide. TB antigen rapid test device (TB Ag) is fast, easy and does not either need skillness of the operator. The kit detects specific secreted antigen M.tuberculosis coded by: RD (Region of Difference) 1, RD2 and RD3. These RD1−3 were found deleted from BCG (Bacille Calmette-Guerine) vaccine strain. In the present study, the diagnostic value of TB Ag was assessed. Sputum samples were examined from 59 suspected tuberculosis patients and 22 non tuberculosis patients. The samples of the suspected tuberculosis patients were collected as three consecutive sputum specimens (spot, morning, spot). The total 199 specimens were examined by sputum smear microscopy and TB Ag. M.tuberculosis culture by using Lowenstein Jensen media, which was used as a gold standard. The sensitivity and specificity of microscopic sputum smear were 83.8% (95% CI: 70.0–89.4) and 96.3% (95% CI: 89.8–98.7), respectively. While, the sensitivity and specificity of TB Ag were 72.6% (95% CI: 63.9–79.9) and 90.9% (95% CI: 72.2–97.5), respectively. The concordance between microscopic sputum smear and TB Ag was 70.8%. TB Ag can be considered as a new diagnostic tool for the diagnosis of pulmonary tuberculosis, especially at the health services where there is no expert technician available for microscopic sputum smear examination.
HITUNG TROMBOSIT DI SINDROM KORONER AKUT TERKAIT LOW MOLECULAR WEIGHT HEPARIN (LMWH) Cyntia Kornelius; Darwati Muhadi; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.376

Abstract

Heparin and Low molecular weight heparin (LMWH) had been used widely for prevention and treatment of thrombosis in the patients with acute coronary syndrome (ACS). Actually, the administration of heparin may caused some adverse effect, such as heparin inducedthrombocytopenia (HIT). This study was aimed to reveal thrombocytes count in patients with ACS who underwent LMWH therapy. An observational study with cohort prospective design was performed in 30 patients with ACS. The thrombocytes count was obtained from complete blood count (CBC) by using haematology analyzer (Sysmex XT 2000i) that performed at Clinical Pathology Laboratory, Dr. Wahidin Sudirohusodo Hospital, Makassar. The obtained data was analyzed with Wilcoxon test. The mean thrombocytes count before the administration of LMWH was 236.800/μL, while mean thrombocytes count after the administration of LMWH was 280.270/μL. Suprisingly this data showed that in general thrombocytes count was increased significantly (P=0.004) five day after starting LMWH therapy. In this study only one (3.3%) of 30 patients who received LMWH had trombocytes count less than 150.000/μL. Based on this study an antibody test to PF4-heparin complex was needed to determined occurance of HIT in this patient. The thrombocytes count was increased significantly (P=0.004) in patients with ACS who had given LMWH therapy.
IMUNISASI PROTEIN ADHESIN 38-KDA MYCOBACTERIUM TUBERCULOSIS LEWAT RONGGA MULUT TERKAIT SEL T CD8+ DI PARU Arthamin, Maimun Z; Gani, Agus A; Issiyah, Nurani; Santoso, Sanarto
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.377

Abstract

The efficacy of Bacillus Calmette-Guerin (BCG), vaccine against tuberculosis (TB), varies widely, from 0 to 90%; and BCG mainly activates CD4+ T cells, but it fails to activate CD8+ T cells. From the previous study, 38-kDa protein is an adhesin protein. CD8+ T cells play the role in controlling Mycobacterium tuberculosis (M.tb) infection and contribute to the memory immunity. The objective of this study was to determine effect of oral immunization by 38-kDa adhesin protein of M.tb to increase the level of CD8+ T cells in the lung of BALB/c mice. This study used an experimental with post test control group design. The mice were divided into six groups (each group consist of 4 samples), where Group 1: were immunization orally with 100 μg 38-kDa adhesin protein of M.tb and 12 μg ISCOMs. Followed by group 2: 100 μg 38-kDa adhesin protein of M.tb, group 3: 50 μg 38-kDa adhesin protein of M.tb and 12 μg ISCOMs, and group 4: 50 μg 38-kDa adhesin protein of M.tb. Group 5: 12 μg ISCOMs. Group 6: Control. In this study was found increased level of CD8+ T cells in the lung of BALB/c mice after orally immunization with 38-kDa adhesin protein of M.tb. The highest level of CD8+ T cells was on group 1, p=0.000. Also there were found significant differences among the immunized groups, except group 2 and 3, as well as group 5 and 6 also. It can be concluded in this study that oral immunization with 38-kDa adhesin protein of M.tuberculosis could increase the level of CD8+ T cells in the lung of BALB/c mice.

Page 1 of 2 | Total Record : 11

 1   2 

Filter by Year

2012 2012


Reset
Filter By Issues
All Issue Vol. 30 No. 1 (2023) Vol. 29 No. 3 (2023) Vol. 29 No. 2 (2023) Vol. 29 No. 1 (2022) Vol 29, No 1 (2022) Vol 28, No 3 (2022) Vol. 28 No. 3 (2022) Vol. 28 No. 2 (2022) Vol 28, No 2 (2022) Vol. 28 No. 1 (2021) Vol 28, No 1 (2021) Vol 27, No 3 (2021) Vol. 27 No. 3 (2021) Vol. 27 No. 2 (2021) Vol 27, No 2 (2021) Vol. 27 No. 1 (2020) Vol 27, No 1 (2020) Vol. 26 No. 3 (2020) Vol 26, No 3 (2020) Vol 26, No 2 (2020) Vol. 26 No. 2 (2020) Vol 26, No 1 (2019) Vol. 26 No. 1 (2019) Vol. 25 No. 3 (2019) Vol 25, No 3 (2019) Vol. 25 No. 2 (2019) Vol 25, No 2 (2019) Vol 25, No 1 (2018) Vol. 25 No. 1 (2018) Vol 24, No 3 (2018) Vol. 24 No. 3 (2018) Vol. 24 No. 2 (2018) Vol 24, No 2 (2018) Vol. 24 No. 1 (2017) Vol 24, No 1 (2017) Vol 23, No 3 (2017) Vol. 23 No. 3 (2017) Vol 23, No 2 (2017) Vol. 23 No. 2 (2017) Vol 23, No 1 (2016) Vol 22, No 3 (2016) Vol 22, No 2 (2016) Vol 22, No 1 (2015) Vol 21, No 3 (2015) Vol 21, No 2 (2015) Vol 21, No 1 (2014) Vol 20, No 3 (2014) Vol 20, No 2 (2014) Vol 20, No 1 (2013) Vol 19, No 3 (2013) Vol 19, No 2 (2013) Vol 19, No 1 (2012) Vol. 19 No. 1 (2012) Vol 18, No 3 (2012) Vol. 18 No. 3 (2012) Vol 18, No 2 (2012) Vol 18, No 1 (2011) Vol. 18 No. 1 (2011) Vol 17, No 3 (2011) Vol 17, No 2 (2011) Vol 17, No 1 (2010) Vol 16, No 3 (2010) Vol 16, No 2 (2010) Vol 16, No 1 (2009) Vol 15, No 3 (2009) Vol 15, No 2 (2009) Vol 15, No 1 (2008) Vol 14, No 3 (2008) Vol 14, No 2 (2008) Vol 14, No 1 (2007) Vol 13, No 3 (2007) Vol 13, No 2 (2007) Vol 13, No 1 (2006) Vol 12, No 3 (2006) Vol 12, No 2 (2005) Vol 12, No 1 (2005) More Issue