cover
Contact Name
I Made Sumarya
Contact Email
sumaryaimade@yahoo.com
Phone
+6281338717933
Journal Mail Official
sumaryaimade@yahoo.com
Editorial Address
Prodi Biologi Fakultas Teknologi Informasi dan Sains Universitas Hindu Indonesia Jln. Sangalangit Tembau Penatih, Denpasar, Bali
Location
Kota denpasar,
Bali
INDONESIA
Jurnal Widya Biologi
ISSN : 20865783     EISSN : 26556456     DOI : -
Jurnal Widya Bioloogi adalah jurnal open access dan ditelaah oleh mitra bestari bidang biologi, bertuuan untuk mempublikasikan artikel hasil penelitian atau pemikiran ilmiah dan kajian pustaka dalam bidang ilmu biologi dan ilmu-ilmu lain yang berkaitan dengan ilmu biologi. Fokus dan scope yang ditelaah adalah biologi sains dan biologi terapan.
Articles 61 Documents
PENGARUH PUPUK TRICHODERMA SP. DENGAN MEDIA TUMBUH BERBEDA TERHADAP PERTUMBUHAN VEGETATIF TANAMAN CABAI MERAH BESAR (Capsicum Frutescens L.) I Wayan Suanda
JURNAL WIDYA BIOLOGI Vol 10 No 01 (2019): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.815 KB) | DOI: 10.32795/widyabiologi.v10i01.228

Abstract

This research aimed to find out the effects of Trichoderma sp. fertilizers with different growing media on the vegetative growth of chili plants and to find out the best growing media to make it grow optimally.This research was conducted in the Biologi Laboratory at FPMIPA IKIP PGRI Bali from April to June, 2018. The research was done in several growing media; treatmen P0 (without the Trichoderma sp. fertilizer), P1 (Trichoderma sp. fertilizer from taro media), P2 (Trichoderma sp. fertilizer from cassava media), P3 (Trichoderma sp. fertilizer from sweet potato media), P4 (Trichoderma sp. fertilizer from corn media), P5 (Trichoderma sp. fertilizer from potato media). These treatments were repeated four times each. The population in this study were all chili pepper seeds of F1 Megatop variety with a total of 100 plants and a sample of 72 plant that were three weeks old. Each group contained of three chili plants. The data collected in this study were about the height and number of leaves on the chili plants. The data were analyzed using the analysis o variance (ANOVA) with F test and a 5% significance level followed by the LSD test.Based on the analysis, there are several effects of Trichoderma sp. fertilizer with different growing media on the vegetative growth of chili plants. The results showed that treatment P4 (Trichoderma sp. fertilizer from corn media) have the most effective and optimal effects on the chili plants.
TROMBOSIT TERENDAH, KENAIKAN HEMATOKRIT DAN KADAR TNF-α SEBAGAI INDIKASI KEPARAHAN INFEKSI VIRUS DENGUE PADA PASIEN DD DAN DBD A.S. Siskayani; I Made Sumarya; N.L.P Kartika Sari
JURNAL WIDYA BIOLOGI Vol 10 No 01 (2019): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.195 KB) | DOI: 10.32795/widyabiologi.v10i01.233

Abstract

Clinical manifestations of dengue virus infection vary from unspecified fever, dengue fever (DF) and dengue hemorrhagic fever (DHF). The fundamental difference in the diagnosis between DF and DHF is plasma leakage, hypotension, thrombocytopenia and hemorrhagic diathesis in DHF. The objectives of the study were to determine the lowest platelet, haematocrit increase, and TNF-α levels in DF and DHF patients as an indication of severity of dengue virus infection. The cross-sectional study was conducted by taking 54 blood samples of patients aged 26-45 years who were infected by dengue virus and grouped into two groups: DF patient group (27 blood samples) and DHF group (27 blood samples). The lowest platelets and hematocrit rise were determined by Hematology Analyzer and the TNF-α levels were determined by ELISA. The data obtained were statistically analyzed by independent Mann-Whitney test. The results showed that the lowest mean platelet counts of DF patients were significantly (p <0.01) higher than those of DHF patients. The mean hematocrit increase in DF patients was significantly (p <0.01) lower than that of the DHF patients and the mean TNF-α levels of DF patients were significantly (p <0.01) lower than the DHF patients. Based on the results of this study it can be concluded that the lowest platelets, hematocrit increase and TNF-α levels are an indication of the severity of dengue virus infection.
DAYA HAMBAT EKSTRAK LIDAH BUAYA (Aloe barbadensis Miller) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus SECARA IN VITRO Pramesti Indah Prabasari; I M Sumarya; N.K.A. Juliasih
JURNAL WIDYA BIOLOGI Vol 10 No 01 (2019): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.68 KB) | DOI: 10.32795/widyabiologi.v10i01.234

Abstract

Aloe vera (Aloe barbadensis Miller) contains anthraquinone compounds, flavonoids and saponins which are thought to have antibacterial effects so as to inhibit bacterial growth. The purpose of the study was to determine the inhibitory power of Aloe barbadensis Miller on the growth of Staphylococcus aureus bacteria. The pure experimental study was conducted with The Randomized Posttest Only Control Group Design using six (6) treatments, namely aloe vera extract with a concentration of 25%, 50%, 75%, 100%, Chloramphenicol 30 mcg antibiotics as a positive control, and sterile aquadest as a control negative for Staphylococcus aureus bacteria grown on Mueller Hinton media. After being treated, it was incubated for 24 hours and measured its inhibitory power. The growth inhibition data of Staphylococcus aureus bacteria in the form of inhibition zone diameter was analyzed statistically by Kruskal Wallis test, at the level of confidence α = 0.05. The results showed that the average inhibitory effect of Aloe vera extract on the growth of Staphylococcus aureus bacteria in concentrations of 25%, 50%, 75% and 100% were 0 ± 0 mm; 7.125 ± 0.1250 mm; 8.375 ± 0.23936 mm; and 9.0 ± 0.40825 mm. Based on the results of the study concluded that aloe vera extract can inhibit the growth of Staphylococcus aureus bacteria. The higher the concentration of Aloe vera extract the greater the inhibitory effect on the growth of Staphylococcus aureus bacteria.
EFEKTIVITAS PEMBERIAN HYDROTHERAPI RENDAM KAKI PADA PENDERITA HIPERTENSI DI BANJAR SRI MANDALA, KELURAHAN DAUHWARU KABUPATEN JEMBRANA N.L.G. Sudaryati; I P. Sudiartawan; Dwi Mertha Adnyana
JURNAL WIDYA BIOLOGI Vol 10 No 01 (2019): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.404 KB) | DOI: 10.32795/widyabiologi.v10i01.235

Abstract

The aim of the study was to determine the effectiveness of giving hydrotherapi foot soak in hypertensive patients. The study was conducted with one group pretestposttest design without a control group by measuring blood pressure (pretest) before being given an intervention in the form of foot soak hydrotherm against 15 people with hypertension in Banjar Sri Mandala, Dauhwaru Village, Jembrana Subregency. Then do the blood pressure measurement again (posttest) after finishing the intervention. After the study was completed, the results showed that before the hydrotherapi foot bath intervention, there were 0% of patients classified as normal, 13.32% in prehypertension category, 60.08% in hypertension category I and 26.60% in hypertension category II. After the intervention was given, there were 13.32% of the patients classified as normal, 66.68% in the prehypertension category, 20.00% in the first category of hypertension and no patients belonging to the second grade hypertension category. There is a decrease of 20-30 mmHg for systolic blood pressure and 0-10 mmHg for diastolic blood pressure after intervention. Based on the results of the study it can be concluded that the hydrotherapi foot bath is effectively used to reduce blood pressure in hypertensive patients in the Banjar Sri Mandala, Dauhwaru Village, Jembrana District.
DAYA HAMBAT EKSTRAK RIMPANG BANGLE (Zingiber purpureum Roxb.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus A Citradewi; I M Sumarya; N.K.A. Juliasih
JURNAL WIDYA BIOLOGI Vol 10 No 01 (2019): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.562 KB) | DOI: 10.32795/widyabiologi.v10i01.236

Abstract

The rhizome of the bangle contains antibacterial compounds including saponins, flavonoids, essential oils, and tannins. The aim of the study was to determine the inhibitory power of bangle rhizome extract on the growth of Staphylococcus aureus bacteria. True research - experiment with posttest design with control group was carried out by giving 6 treatment groups, namely negative control group (K-) using aquadest, positive control group (K +) using 30 mcg Chloramphenicol antibiotics, 25% ( PE1), 50% (PE2), 75% (PE3), and 100% (PE4) of concentration of rhizome extract treatment. After the treatment is complete, the inhibition is measured. The research data were analyzed statistically by Kruskall Wallis non parametric test and Sperman Rang correlation test. The results showed that the average inhibition of bangle rhizomes in each group in groups K-, K+, PE1, PE2, PE3, and PE4 were 0.000 ± 0.000, 25.000 ± 0.408, 14.250 ± 0.479, 16.500 ± 0.289, 17.750 ± 0.250, 19,500 ± 0.289 differ significantly (p <0.05). There was a very significant positive correlation between the concentration of rhizome bangle extract and the growth inhibition of Staphylococcus aureus bacteria (r count of 0.967 (**)> r table which was 0.503 at α = 0.05 and n = 24 or p <0.01). Conclusion: bangle rhizome extract at concentrations of 25%, 50%, 75%, and 100% has a strong inhibitory power against the growth of Staphylococcus aureus bacteria. The higher the concentration the greater the inhibitory power.
KONTAMINASI SOIL TRANSMITTED HELMITHS PADA HELAIAN DAUN DAN CARA PENCUCIAN SAYURAN KUBIS DI PASAR TRADISIONAL KABUPATEN TABANAN N.K. Tri Widianingsih; E.D. Yuliana; A.A.K. Suardana
JURNAL WIDYA BIOLOGI Vol 10 No 01 (2019): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.751 KB) | DOI: 10.32795/widyabiologi.v10i01.237

Abstract

This study aimed to determine the types of STH that contaminate cabbages in the Tabnan traditional market, and to determine the effect of leaf position and washing method on STH contamination. This study using completely randomized experimental design. The research was carried out in the Tabanan traditional market. A total of 28 cabbage were taken in 10 traditional markets in Tabanan Regency. Laboratory tests were conducted to determine the presence of STH were carried out by 0.2% NaOH sedimentation technique and centrifugation. The STH examination method uses the Kato-Katz method. The results of the study found soil transmitted helminth larvae on the strands of the outer cabbage leaves without washing and stagnant water washing. STH larvae found were filariform larvae of Necator americanus and Ancylostoma duodenale on the outer strands without washing. Whereas in the outermost strands with stagnant water washing only filariform larvae of Necator americanus were found. When compared to the average number of three replications, in the outermost cabbage strands without washing found more larvae compared to stagnant water washing. In the outermost strands by washing the running water there was no STH contamination nor did the middle strands and inner strands, either without washing, washing in stagnant water or washing water flow. However before consumption, cabbage should be washed with running water, to avoid infection with STH through the oral route.
GAMBARAN HISTOPATOLOGI HATI TIKUS WISTAR (Rattus norvegicus) PASCA PEMBERIAN MONOSODIUM GLUTAMAT Angga Baskara; I.N. Arsana; N.K.A. Juliasih
JURNAL WIDYA BIOLOGI Vol 10 No 01 (2019): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (382.749 KB) | DOI: 10.32795/widyabiologi.v10i01.238

Abstract

Monosodium glutamate (MSG) has been used very widely in the community as a food flavoring, and is thought to have a detrimental effect. This study aims to determine the effect of MSG on liver histopathology. 28 Wistar rats were used in this study. Rats were grouped into four groups: control (K), MSG-1 (MSG dose 1.5 mg / g bodyweight), MSG-2 (MSG dose 3.5 mg / g bw), and MSG-3 (MSG dose 4, 5 mg / g bw). MSG is given every day for 30 days. At the end of the treatment, the rat were euthanized and dissected for taking the liver. Histology preparation were made using the paraffin method and stained with Hematoxyclin Eosin (HE) staining. Kruskal Wallis and Mann Whitney test were used for statistical analysis. The results showed that the dose of MSG was 1.5; 3; and 4.5 mg / g bw causes liver damage with the same level of damage in the form of focused degeneration in one place. The higher the dose, the more degeneration of focused on one place which were found. Liver tissue damage scores in group K, MSG-1, MSG-2, and MSG-3 were 0; 0.4; 0.42857, and 0.45714. Conclusion, the administration of MGS has an effect on liver histopathology.
HIPERURISEMIA SEBAGAI FAKTOR RISIKO PENYAKIT KARDIOVASKULAR MELALUI MEKANISME STRES OKSIDATIF I Made Sumarya
JURNAL WIDYA BIOLOGI Vol 10 No 02 (2019): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.628 KB) | DOI: 10.32795/widyabiologi.v10i02.406

Abstract

Hiperurisemia sudah dikenal sejak dahulu, dan mendapat perhatian medis karena berhubungan dengan berbagai penyakit seperti tekanan darah tinggi, aterosklerosis, penyakit ginjal, diabetes melitus dan penyakit kardiovaskular. Hubungan ini mungkin memiliki penyebab yang signifikan namun, tidak ada mekanisma yang jelas hiperurisemia menyebabkan penyakit kardiovaskular. Hiperurisemia sebagai faktor risiko penyakit kardiovaskular dengan memberikan efek vaskular secara langsung dan menyebabkan kerusakan vaskular. Berdasarkan hasil-hasil penelitian diketahui bahwa Asam urat menyebabkan proliferasi endotel, produksi angiotensin II dan peningkatan stres oksidatif (ROS). Asam urat pada konsentrasi tinggi (hiperurisemia) menimbulkan stress oksidatif dengan meningkatkan pembentukan ROS, menginduksi perubahan vaskular melalui aktivasi jalur sinyal sensitif redoks mengaktifkan mitogen activated protein kinase (MAPKs), tirosin kinase dan faktor transkripsi (NFKB, AP-1, dan HIF-1) merangsang Volume 10 Nomor 02 Oktober 2019 P ISSN : 2086-5783 E ISSN : 2655-6456 WIDYA BIOLOGI 88 pembentukan monocyte chemoattractant protein-1 (MCP-1), inflamasi dan meningkatkan siklooksigenase 2 (COX-2). Hiperurisemia sebagai faktor risiko penyakit kardiovaskular terkait dengan aktivitas inflamasi, aktivasi sistim rennin angiotensin aldosteron (RAAS), disfungsi endotel, proliferasi vascular smooth muscle cell (VSMC) dan tingginya tekanan darah. Simpulan: hiperurisemia sebagai faktor risiko penyakit kardiovaskular yaitu dengan menyebabkan kerusakan vaskular melalui mekanisma peningkatan pembentukan ROS, mengaktifkan mitogen activated protein kinase (MAPKs), tirosin kinase dan faktor transkripsi (NFKB, AP-1, dan HIF-1) merangsang pembentukan monocyte chemoattractant protein-1 (MCP-1), inflamasi dan meningkatkan siklooksigenase 2 (COX2).
KARAKTERISASI MORFOLOGIS Trichoderma sp. ISOLAT JB DAN DAYA HAMBATNYA TERHADAP JAMUR Fusarium sp. PENYEBAB PENYAKIT LAYU DAN JAMUR AKAR PUTIH PADA BEBERAPA TANAMAN I Wayan Suanda
JURNAL WIDYA BIOLOGI Vol 10 No 02 (2019): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (819.193 KB) | DOI: 10.32795/widyabiologi.v10i02.407

Abstract

Penelitian ini bertujuan untuk mengetahui karakterisasi morfologis Trichoderma sp. isolat JB dan daya hambatnya terhadap jamur Fusarium sp. penyebab penyakit layu dan jamur akar putih (JAP) pada tanaman cengkeh dan tanaman asem. Penelitian dilakukan di Laboratorium Hama dan Penyakit Tumbuhan Fakultas Pertanian, Universitas Udayana. Variabel observasi berupa karakteristik makroskopik, meliputi warna koloni, elevasi, bentuk tepi koloni dan karakteristik mikroskopis termasuk bentuk konidiofor, fialid dan konidia. Hasil penelitian menunjukkan bahwa Trichoderma sp. isolat JB secara in-vitro pada umur 5 HSI mampu menghambat pertumbuhan koloni jamur Fusarium sp. penyebab penyakit layu pada tanaman stroberi = 63,33%, tanaman kacang panjang = 50,66%, tanaman cabai 66,67%, tanaman tomat = 89,47% dan mampu menekan pertumbuhan koloni JAP pada tanaman cengkeh sebesar 90,14 % serta JAP pada tanaman asem sebesar 81,48%.
STUDI POTENSI DAUN SUJI (DRACAENA ANGUSTIFOLIA) SEBAGAI BAHAN OBAT : SEBUAH KAJIAN Putri Sri Andila; Tri Warseno
JURNAL WIDYA BIOLOGI Vol 10 No 02 (2019): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.182 KB) | DOI: 10.32795/widyabiologi.v10i02.408

Abstract

Daun Suji (Dracaena angustifolia) merupakan tanaman asli Indonesia yang secara turun temurun telah dimanfaatkan sebagai pewarna makanan alami. Akan tetapi kajian etnobotani dan farmakologi mengungkapkan bahwa tanaman ini memiliki potensi sebagai bahan obat. Tujuan dari studi ini adalah untuk mengungkap literasi potensi Daun Suji (D. angustifolia) sebagai bahan obat. Studi etnobotani menunjukkan bahwa Daun Suji telah digunakan sebagai obat tradisional di beberapa Negara di Asia Tenggara yaitu sebagai obat gigitan serangga, obat sakit perut, asma, sesak nafas, kencing nanah, beri beri, nyeri haid, gastritis, anti konstipasi, penambah nafsu makan dan obat untuk orang yang menderita pengurangan berat badan. Sedangkan kajian farmakologi menunjukkan bahwa Daun Suji memiliki potensi sebagai penurun kolesterol, penurun reaksi anafilaksis, antioksidan, antiinflamasi, antiproliferasi, antibakteri, dan antijamur.