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All Journal INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Veteriner Ecotrophic, Journal of Environmental Science Jurnal Kajian Bali JURNAL PETERNAKAN Jurnal Widya Biologi Journal of Tropical Ethnobiology Jurnal Bali Membangun Bali
I Nyoman Arsana
Program Studi Biologi, Fakultas Teknologi Informasi Dan Sains, Universitas Hindu Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Social Sciences Transportation Veterinary Other

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GARCINIA MANGOSTANA L. RIND EXTRACT AND PHYSICAL TRAINING REDUCE OXIDATIVE STRESS IN WISTAR RATS DURING MAXIMAL PHYSICAL ACTIVITY Arsana, I N.; Adiputra, N.; Pangkahila, J. A.; Putra-Manuaba, and I. B.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol 7 No 2 (2013): IJBS Vol2 No2
Publisher : Udayana University

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Abstract

Oxidative stress is a condition of imbalance between the production of free radicals or reactive oxygen species (ROS) and antioxidants, in which the levels of free radicals higher than antioxidants. One causes of oxidative stress is the maximum physical activity. Oxidative stress can be reduced by antioxidants. One source of antioxidants is mangosteen rind (Garcinia mangostana L). This study aimed to investigate the role of mangosteen rind extract and physical training in reducing Malondialdehyde (MDA, increasing Superoxide dismutase (SOD) and Glutathione Peroxidase (GPx). This study used a randomized block design with factorial pattern of 6 x 2 with four repetitions. The first treatments are mangosteen rind extract with doses; 0, 50, 100; 200; 300, and 400 mg/kg bodyweight/day for four weeks. The second treatments are the physical training consists of; without and with physical training. MDA, SOD and GPx were assessed in the end of treatment. Data were analyzed with GLZ. The results showed that average of MDA, SOD and GPx different significantly (p<0.05) after administration of extracts as well as physical training. Extracts and physical training concurrently also showed effect significant (p<0.05). However, at doses 0 until 300 mg/kg bodyweight, MDA levels more higher while SOD and GPx lower significantly (p<0.05) at training group compared with without training. Whereas at dose 400 mg/kg bw, MDA lower at training (p>0.05), while SOD and GPx were recorded significantly (p<0.05) higher than the group without training. Overall, it could be concluded that physical training with administration of mangosteen rind extract reduce oxidative stress through reduction of MDA, as well as increased both SOD and GPx.
Titer Hemaglutinasi dan Kematian Embrio pada Telur Spesific Antibody Negative (SAN) dengan Usia yang Berbeda Saat Inokulasi Virus Avian Influenza Fauzi Rahmat Kurniawan; I Nyoman Arsana; I Gede Ketut Adiputra
Jurnal Peternakan Vol 19, No 1 (2022): Februari 2022
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v19i1.15101

Abstract

ABSTRAK. Penyakit Avian Influenza (AI) tidak hanya menginfeksi berbagai spesies unggas tetapi juga dapat menular ke manusia. Penelitian ini bertujuan untuk mengkaji titer hemaglutinasi dan kematian embrio pada telur Spesific Antibody Negative (SAN) dengan usia yang berbeda saat inokulasi virus Avian Influenza (AI). Penelitian menggunakan rancangan acak lengkap dengan empat perlakuan yaitu telur SAN usia 7 hari, 9 hari, 11 hari dan 13 hari. Perlakuan diulang sebanyak 6 kali sehingga total sampel telur SAN yang digunakan adalah sebanyak 24 telur. Hasil penelitian menunjukkan bahwa usia telur SAN berpengaruh secara signifikan terhadap titer hemaglutinasi dan waktu kematian embrio ayam. Rerata titer hemaglutinasi telur SAN usia 9; 11, dan 13 hari berturut-turut adalah 597,33 ± 85,33 HAU/25µl; 1.322,67 ± 339,73 HAU/25μl, dan 256 ± 85,86 HAU/25µl. Pada telur SAN usia 7 hari tidak ada titer hemaglutinasi pascainokulasi virus. Waktu kematian embrio pada telur SAN usia 7 terjadi satu hari pascainokulasi virus AI, sedangkan usia 9; 11, dan 13 hari terjadi dua hari pascainokulasi virus AI. Kesimpulan, titer hemaglutinasi dan kematian embrio telur SAN berbeda tergantung pada usia telur SAN, dan usia telur SAN yang paling baik digunakan untuk inokulasi virus AI adalah usia 11 hari. Kata kunci: Avian influenza, kematian embrio, titer hemaglutinasi, telur SAN. Hemagglutination Titers and Embryonic Death in Specific Antibody Negative (SAN) Eggs with Different Ages at The Time of Avian Influenza (AI) Virus Inoculation ABSTRACT. Avian influenza (AI) not only infects various species of aves but also can be transmitted to humans. This study was aim to examine hemagglutination titers and embryonic death in Specific Antibody Negative (SAN) eggs with different ages at the time of Avian Influenza (AI) virus inoculation. The study used a completely randomized design with four treatment groups, namely SAN eggs 7 days old, 9 days, 11 days, and 13 days. The treatment was repeated 6 times so the total sample of SAN eggs used was 24 eggs. The results showed that the age of SAN eggs had a significant effect on the hemagglutination titer and the time of death of chicken embryos. Average hemagglutination titer SAN eggs age 9; 11, and 13 days respectively were 597.33 ± 85.33 HAU/25µl; 1,322.67 ± 339.73 HAU/25μl, and 256 ± 85.86 HAU/25µl. There was no hemagglutination titer in SAN eggs aged 7 days post-inoculation AI virus. The time of embryonic death in SAN eggs aged 7 occurred one-day post-inoculation AI virus, while age 9; 11, and 13 days occurred two days post-inoculation AI virus. In conclusion, hemagglutination titers and embryonic mortality differed depending on the age of the SAN eggs, and the best age to be used for AI virus inoculation was 11 days.
Keragaman Tanaman Obat dalam Lontar “Taru Pramana” dan Pemanfaatannya untuk Pengobatan Tradisional Bali I Nyoman Arsana
Jurnal Kajian Bali (Journal of Bali Studies) Vol 9 No 1 (2019): WACANA KRITIS BUDAYA BALI
Publisher : Pusat Kajian Bali Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (817.913 KB) | DOI: 10.24843/JKB.2019.v09.i01.p12

Abstract

“Taru Pramana” lontar manuscripts contain medicinal plant diversity and it uses for traditional system of Balinese medicine. This study aims to describe medicinal plant diversity in “Taru Pramana” lontar manuscripts and its use for traditional system of Balinese medicine. This research uses the library method. The unit of analysis is the “Taru Pramana” lontar manuscript from collection of the Leiden University Library, Netherlands. The data obtained were analyzed descriptively. The ethnosystem approach is used as the main approach in this analysis, which places an emic perspective on the relationship between humans and plants. A total of 182 plant species are used in traditional Balinese medicine, mostly including family members of euphorbiaceae, moraceae, fabaceae, and zingiberaceae. The parts used were leaves, shoots, stem bark, roots, fruit, sap, tuber and used them into various forms of medicine such as loloh, boreh, sembar, tutuh, tempel, and ses. The practice of the use of medicinal plants in traditional Balinese medicine is based on a strong belief and knowledge system.
Pemulihan Kadar Glikogen Serta Peningkatan Konsumsi Glukosa dan Trigliserida Saat Aktivitas Fisik Pascapemberian Ekstrak Kulit Buah Manggis (GLYCOGEN RECOVERY AND INCREASE CONSUMPTION OF GLUCOSE AND TRIGLYCERIDE DURING PHYSICAL ACTIVITIES AFTER ADMINISTRA I Nyoman Arsana; Ni Ketut Ayu Juliasih
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was aimed to investigate the effect of mangosteen rind on the glycogen recovery of themuscle and the liver, and the glucose and the triglyceride consumption during physical activities. ARandomized Block Design was applied with four treatments: control (K), physical activity (KF), physicalactivity and extract (FE),extract (E). The extract dosage was 400 mg/kg bodyweight/day administered forfour weeks. The assessed variables were the muscle glycogen, the liver glycogen, the blood glycogen, and thetriglyceride in the end of the treatments. Based on the research results, it was found that the muscleglycogen, the blood glucose, and the triglyceride of the E group were lower, whereas the liver glycogen wassignificantly higher than that of the control (K) group. The means of the muscle glycogen, the liver glycogen, the blood glucose, and the triglyceride of the control (K) group were respectively 0.41±0,01 ?g/25mg, 0.22±0.01?g/25mg, 85.89±2.45 mg/dL, and 32.00±3.38 mg/dL whereas of the E group were 0.39±0.01 ?g/25mg,0.26±0.02 ?g/25mg, 75.84±2.29 mg/dL, and 23.39±2.08 mg/dL. After a physical activity of the KF group,the muscle glycogen and the liver glycogen decreased, however the blood glucose and the triglycerideincreased significantly if compared to those of the control (K) group. The means of the KF group wererespectively 0.14±0.01 ?g/25mg, 0.09±0.01 ?g/25mg, 164.73±11.07 mg/dL, and 66.31±2.96 mg/dL. Afterthe administration of the extract to the FE group, their muscle and liver glycogen increased, but the glucoseand the triglyceride decreased more significant than those of the KF. The means of the FE group were0.35±0.01 ?g/25mg, 0.19±0.01 ?g/25mg, 107.05±7.79 mg/dL, and 40.00±2.30 mg/dL respectively. Theseresults showed that during the physical activities, the energy was taken from the muscle and liver glycogen,whereas the glucose and the triglyceride were mobilized into the blood as the reserve source. After theextract administration, the source of the energy was taken back to the muscle and the liver, and then theglucose and the triglyceride were utilized. The compound within the extract is assumed to have influenceon the activities of the enzymes involved in the metabolism of the carbohydrate and the fat. It can beconcluded that the mangosteen rind extract recovers the muscle and the liver glycogen as well as increasingthe glucose and the triglyceride consumption during the physical activities.
GAMBARAN HISTOPATOLOGI HATI TIKUS WISTAR (Rattus norvegicus) PASCA PEMBERIAN MONOSODIUM GLUTAMAT Angga Baskara; I.N. Arsana; N.K.A. Juliasih
JURNAL WIDYA BIOLOGI Vol 10 No 01 (2019): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (382.749 KB) | DOI: 10.32795/widyabiologi.v10i01.238

Abstract

Monosodium glutamate (MSG) has been used very widely in the community as a food flavoring, and is thought to have a detrimental effect. This study aims to determine the effect of MSG on liver histopathology. 28 Wistar rats were used in this study. Rats were grouped into four groups: control (K), MSG-1 (MSG dose 1.5 mg / g bodyweight), MSG-2 (MSG dose 3.5 mg / g bw), and MSG-3 (MSG dose 4, 5 mg / g bw). MSG is given every day for 30 days. At the end of the treatment, the rat were euthanized and dissected for taking the liver. Histology preparation were made using the paraffin method and stained with Hematoxyclin Eosin (HE) staining. Kruskal Wallis and Mann Whitney test were used for statistical analysis. The results showed that the dose of MSG was 1.5; 3; and 4.5 mg / g bw causes liver damage with the same level of damage in the form of focused degeneration in one place. The higher the dose, the more degeneration of focused on one place which were found. Liver tissue damage scores in group K, MSG-1, MSG-2, and MSG-3 were 0; 0.4; 0.42857, and 0.45714. Conclusion, the administration of MGS has an effect on liver histopathology.
KOMPOSISI UKURAN DAN JENIS KELAMIN IKAN HIU KARANG SIRIP HITAM (CARCHARHINUS MELANOPTERUS) KOMODITAS EKSPOR BALI Arisandi Arisandi; Arsana I N.; Sudaryati N.L.G.
JURNAL WIDYA BIOLOGI Vol 11 No 01 (2020): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.763 KB) | DOI: 10.32795/widyabiologi.v11i01.570

Abstract

Penelitian bertujuan untuk mengetahui komposisi ukuran dan jenis kelamin ikan hiu karang sirip hitam (Carcharhinus melanopterus). Penelitian dilaksanakan menggunakan metode observasi lapangan. Sampel didapat dari sepuluh perusahaan eksportir ikan hiu di Provinsi Bali. Variabel yang diukur adalah panjang tubuh dan jenis kelamin ikan hiu. Panjang tubuh diukur dari ujung mulut sampai dengan ujung ekor. Jenis kelamin dibedakan berdasarkan adanya claspers. Data yang diperoleh kemudian dianalisis secara deskriptif. Hasil penelitian mendapatkan sebanyak 208 ekor ikan hiu karang sirip hitam (Carcharhinus melanopterus) komoditas ekspor Bali. Sebanyak 149 ekor (71,63 %) ikan berjenis kelamin betina, dengan panjang tubuh rata-rata 48 cm. Sedangkan ikan hiu berjenis kelamin jantan sebanyak 59 ekor (38,37 %) dengan rata-rata berukuran 50 cm. Kesimpulan, komposisi ukuran tubuh dan jenis kelamin ikan hiu karang sirip hitam (Carcharhinus melanopterus) komoditas ekspor Bali bervariasi berdasarkan jenis kelamin.
KADAR GULA DARAH SEBAGAI FAKTOR RISIKO PENYAKIT GINJAL PADA PASIEN DIABETES MELITUS TIPE 2 DI POLI DALAM RSUD BANGLI Melia-Arisanti N. K.; Sumarya I M.; Arsana I N.
JURNAL WIDYA BIOLOGI Vol 11 No 01 (2020): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.349 KB) | DOI: 10.32795/widyabiologi.v11i01.571

Abstract

Diabetes Melitus adalah penyakit metabolik menahun akibat pankreas tidak dapat memproduksi cukup insulin atau tubuh tidak dapat menggunakan insulin yang diproduksi secara efektif sehingga terjadi kelebihan gula di dalam darah (hiperglikemia). Diabetes Melitus merupakan penyebab utama penyakit ginjal stadium akhir. Tujuan dari penelitian adalah untuk mengetahui kadar gula darah merupakan faktor risiko penyakit ginjal pada pasien Diabetes Melitus Tipe 2 di poli dalam RSUD Bangli. Penelitian Survei Case Control dengan pendekatan retrospektif dilaksanakan pada bulan Mei - Juli 2018 di bagian rekam medik RSUD Bangli terhadap 326 pasien Diabetes Melitus Tipe 2 yang dikelompokkan menjadi dua kelompok yaitu Kelompok Kasus dengan penyakit ginjal dan Kelompok Kontrol tanpa penyakit ginjal dengan mencek kadar gula darahnya. Analisis data secara statistik dengan uji Chi-Square pada tingkat kepercayaan 95% untuk menentukan faktor resiko Odd Ratio. Hasil penelitian menunjukkan bahwa ada hubungan yang sangat kuat antara kadar gula darah yang tinggi dengan penyakit ginjal pada pasien Diabetes Melitus Tipe 2 dan kadar gula darah yang tinggi merupakan faktor risiko terjadinya penyakit ginjal sebesar 3,480 kali lebih besar dibandingkan dengan kadar gula darah rendah pada pasien Diabetes Melitus Tipe 2 (OR=3,480; CI 95% = 1,889-6,412; p=0,0001). Simpulan kadar gula darah yang tinggi merupakan faktor risiko terjadinya penyakit ginjal pada pasien Diabetes Melitus Tipe 2 di RSUD Bangli
FITOREMEDIASI DENGAN TANAMAN AIR UNTUK MENGOLAH AIR LIMBAH DOMESTIK Pande Kadek Yusika Ryanita; I Nyoman Arsana; Ni Ketut Ayu Juliasih
JURNAL WIDYA BIOLOGI Vol 11 No 2 (2020): Widya Biologi
Publisher : UNHI Press

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Abstract

Kepadatan jumlah penduduk meningkatkan bahan buangan, termasuk bahan buangancair berupa air limbah domestik. Air limbah domestik mengandung bahan organik yangtinggi sehingga dapat mencemari perairan. Oleh karena itu perlu dilakukan pengolahanyang efisien. Salah satu pengolahan air limbah domestik tersebut adalah fitoremediasi.Penelitian ini bertujuan untuk mengetahui perbedaan kadar pH,COD, BOD, TSS danMinyak-Lemak pada keempat perlakukan fitoremediasi dengan menggunakan tanamaneceng gondok, kapu-kapu, kangkung air dan paku air. Penelitian ini menggunakan desainpenelitian pre-test dan post-test dengan Rancangan Acak Lengkap (RAL). Perlakuan padapenelitian ini dengan memberikan empat jenis tanaman dengan massa 250 gram dan lamafitoremediasi selama satu hari dengan pengulangan sebanyak 5 kali. Sehingga, totalsampel menjadi 25 buah. Setelah fitoremediasi, prosentase penurunan pH tertinggi terjadipada tanaman kapu-kapu sebesar 18.62%. Selanjutnya prosentase penurunan TSS,COD,BOD dan minyak-lemak paling tinggi terjadi pada paku air secara berturut-turut sebesar69.80%, 77.42%, 82.69% dan 38.81%. Simpulan, fitoremediasi dengan tanaman air(eceng gondok, kapu-kapu, kangkung air dan paku air) dapat menurunkan kadar pH, TSS,COD, BOD dan minyak-lemak dengan efektifitas yang berbeda dimana, paku air memilikiprosentase tertinggi pada parameter TSS, COD, BOD, dan minyak-lemak.
KERAGAMAN TUMBUHAN DALAM UPAKARA CARU PANCA SATA Ni Komang Madiasih; I Nyoman Arsana; I Gusti Ayu Ketut Artatik
JURNAL WIDYA BIOLOGI Vol 11 No 2 (2020): Widya Biologi
Publisher : UNHI Press

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Abstract

Penelitian bertujuan untuk mengetahui jenis dan bagian tumbuhan serta bahan lain selaintumbuhan yang digunakan sebagai bahan upakara caru panca sata. Penelitian dilakukandi Desa Pejeng, Kecamtan Tampaksiring, Kabupaten Gianyar. Metode yang digunakanadalah metoda Purposive sampling dengan cara wawancara, observasi, dokumentasibahan banten. Tumbuhan diidentifikasi untuk mengetahui nama jenis. Wawancaradilakukan terhadap responden diantaranya sulinggih, serati, dan masyarakat yang sedangmelaksanakan upacara caru panca sata. Hasil penelitian menemukan 63 jenis tumbuhan(34 famili). Tumbuhan yang paling banyak digunakan adalah dari suku Poaceae. Bagiantumbuhan yang digunakan adalah daun, buah, biji, umbi, bunga dan batang. Bagian yangpaling banyak digunakan adalah daun. Sebanyak 22 jenis bahan lain selain tumbuhanjuga digunakan dalam caru panca sata.
KONSUMSI TABLET FE MENURUNKAN PREVALENSI ANEMIA DAN MENINGKATKAN KADAR HEMOGLOBIN IBU HAMIL DI UPTD PUSKESMAS TAMPAKSIRING II Dewa Ayu Made Novia Dewi; I Nyoman Arsana; I Wayan Suarda
JURNAL WIDYA BIOLOGI Vol 12 No 02 (2021): Widya Biologi
Publisher : UNHI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32795/widyabiologi.v12i02.2141

Abstract

Anemia dapat diartikan sebagai kurangnya kadar haemoglobin (Hb) dalam darah terutama akibat kurangnya zat besi (Fe). Penelitian ini bertujuan untuk mengetahui prevalensi anemia serta pengaruh konsumsi tablet Fe terhadap kadar hemoglobin pada ibu hamil di UPTD Puskesmas Tampaksiring II. Penelitian menggunakan rancagan cross sectional. Sampel penelitian adalah semua ibu hamil yang mendapat asupan tablet Fe dan melakukan pemeriksaan hemoglobin di UPTD Puskesmas Tampaksiring II dalam periode Januari 2019 sampai dengan April 2021. Prevalensi anemia dihitung dengan menentukan prosentase anemia dari seluruh sampel. Data yang diperoleh dianalisis dengan analisis wilcoxon rank test. Hasil penelitian menunjukkan bahwa prevalensi ibu hamil yang mengalami anemia sebelum konsumsi tablet Fe sebesar 18,8% atau 73 orang dari 389 ibu hamil, sedangkan prevalensi ibu hamil yang mengalami anemia setelah konsumsi tablet Fe menurun menjadi sebesar 0,3% atau hanya terjadi pada 1 orang dari 389 ibu hamil. Konsumsi Table Fe mampu meningkatkan kadar haemoglobin secara signifikan (p<0,01; Wilcoxon Signed Rank Test), dimana rata-rata kadar Hb sebelum konsumsi tablet Fe sebesar 11,18 g/dl, sedangkan rata-rata kada Hb sesudah konsumsi tablet Fe meningkat menjadi 12,31 g/dl. Kesimpulan, Konsumsi tablet Fe menurunkan prevalensi anemia ibu hamil periode Januari 2019 sampai dengan April 2021 dan meningkatkan kadar hemoglobin pada ibu hamil di UPTD Puskesmas Tampaksiring II.
Co-Authors and I. B. Putra-Manuaba Angga Baskara Arisandi Arisandi Ayu Meiliana Laksmi Dewi Dewa Ayu Made Novia Dewi Fauzi Rahmat Kurniawan I Gede Ketut Adiputra I Gusti Ayu Ketut Artatik I K. P Astawa I Made Agus Gelgel Wirasuta I Putu Sudiartawan I W. Wahyudi I Wayan Martadi Santika I Wayan Suarda Ida Bagus Putra Suta Ida Bagus Wiryanatha J. A. Pangkahila Luh Putu Arishanti W. Melia-Arisanti N. K. N. Adiputra N.K.A. Juliasih Ni Kadek Warditiani Ni Ketut Ayu Juliasih Ni Ketut Ayu Juliasih Ni Ketut Ayu Juliasih Ni Komang Madiasih Ni Luh Gede Sudaryati Ni Made Widiastuti Ni Nyoman Sri Puspa Adi Ni Nyoman Triyani Pande Kadek Yusika Ryanita Pande Made Nova Armita Putu Alit Wardana Putu Lakustini Cahyaningrum Sudaryati N.L.G. Sumarya I M.