Garuda - Garba Rujukan Digital

Formulation and Physical Characterization of Essential Oil Bangle (Zingiber cassumunar Roxb.) Nanoemulsion Gel Purwanto, Ungsari Rizki Eka; Sholikhah, MarÃ¢â‚¬â„¢atus; Munisih, Siti
Journal of Science and Technology Research for Pharmacy Vol 1 No 1 (2021)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jstrp.v1i1.43500

Background: Bangle rhizome (Zingiber cassumunar Roxb) essential oil as long been used by Indonesian and often used by postpartum women, as a treatment for abdominal obesity. The formulation of bangle essential oil into topical preparations has not been widely reported. So far, bangle essential oil is not available in pharmaceutical dosage form. The users immediately apply essential oil to the abdominal area and previous study showed that the terpinene-4-ol only diffused 0,39% from 2 mg/cm2 of Bangle essential oil through skin membrane. The development of topical bangle essential oil is necessary to increase the effectiveness of the delivery of active substances through the skin and the comfort of the user. In order to increase the penetration of the terpinen-4-ol, the Bangle essential oil was formulated in the form of a nanoemulsion and then the nanoemulsion was dispersed into a gel base to increase user convenience. Aim: The aim of the present study was to investigate the potential of nanoemulsion formulations for Bangle essential oil and to know the optimum the composition of surfactants and cosurfactants in nanoemulsion system. This study also aims to determine the effect of base gel used in the nanoemulsion gel formulation on the appropriate physical properties. Method: The pre-emulsion was made beforehand with oil and a mixture of surfactant and cosurfactant. With the help of a Design ExpertÂ® ver. 10.0.1 software, component of sufactant and co-surfactant were optimized. The design formula was divided into seven nanoemulsion formulas with a ratio of Polysorbate 80: PEG 400, F1 (1: 8), F2 (8: 1), F3 (6,25: 2,75), F4 (8: 1), F5 (1: 8), F6 (1: 8), F7 (4,5: 4,5). The optimum consentration of Polysorbate 80 as surfactant and PEG 400 as co-surfactants in Bangle essential oil nanoemulsion was analyzed using simplex lattice design methode. The optimal formula was further evaluated for its distribution, droplet size, and zeta potential. Distilled water was used as the aqueous phase in nanoemulsion. The amount of aqueous phase: the amount of pre-emulsion is 9 : 1. The optimum nanoemulsion formula then dispersed in carbophol hydrogel to be nanoemulsion gel. Physical characterization of Bangle nanoemulsion gel like a pH, adhesion power, spreadability, viscosity before and after cycling test had been investigated. Result: The optimum composition of surfactant Tween 80 and co-surfactan PEG 400 was 7.659: 1.34. The results showed that the optimal formula with 2% Bangle Essential Oil Loaded in Nanoemulsion had the Z-average 95,2 nm with Index Polidispersity 0,176 and Zeta Potensial -0,1 mV. Bangle Nanoemulsion-Gel had a homogenous organoleptic, spreadability in 6,69 + 0.85 cm, adhesion power in 2.14 + 0.29 seconds, viscosity 5969 + 32.03 cps, and pH 6,58 + 0,29. Conclusion: Bangle Essential Oil Nanoemulsion-Gel formulation has the good physical characteristics and potentially be used as topical dosage forms. A further study is needed to determine the effectiveness of the treatment of abdominal obesity by topical administration of this preparation.

Analysis Tolerance of Monosodium Glutamate (MSG) In instant noodles With Uv-Vis Spectrophotometry Rachma, Firstca Aulia; Saptawati, Tunik
Journal of Science and Technology Research for Pharmacy Vol 1 No 1 (2021)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jstrp.v1i1.43568

Background: MSG has become one of the most widely used food flavorings in the world, the FDA (Food and Drug Administration) reported an average per capita intake of MSG in the United States of 550 mg / day in 1973. The use of MSG as a food additive has been regulated by its use. WHO. This rule stipulates that daily consumption of MSG per person should not exceed the safe threshold of 120 mg / kg / day. MSG is proven to be a risk factor for obesity. The increase in body weight in mice occurred after MSG was given as much as 15-30 mg / kg or the equivalent of 1-2 g / person. Based on the description above, it is known that there is a significant influence of MSG in the health sector if consumed in excess. Therefore, researchers are interested in conducting further research related to MSG levels in instant noodle samples in the Semarang city area. Aim: The purpose of this study was to test the MSG levels in instant noodles with a spectrophotometer. Method: The tools used are a UV-Vis spectrophotometer (Shimadzu), NaCl and MSG salts. MSG used in instant noodles, 0.5% Ninhydrin Reagent. Result: MSG levels from each of the 10 samples of instant noodles that are traded in Semarang City, the highest levels are found in sample J of 4.70% and the lowest MSG levels are in sample G of 2.90%. Conclusion: Instant noodles sold in the city of Semarang are positive for Monosodium glutamate (MSG) and The results of quantitative analysis, where the MSG levels contained in each sample of instant noodles in Semarang City show that it is still below the standard for adults.

Formulation and Evaluation of Antioxidant Peel-Off Mask Ethanol Extract Sarang Semut (Myrmecodia sp.) Using DPPH 2,2-Diphenyl-1-picrylhydrazyl Method Ayuningtyas, Nurista Dida; Febrianto, Yahya; Prasetyo, Alno
Journal of Science and Technology Research for Pharmacy Vol 1 No 1 (2021)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jstrp.v1i1.44159

Background: Myrmecodia sp extract is known to be effective as an antioxidant on a concentration of 5-50 ppm. Antioxidants are substances that can scavenge free radicals. Free radicals can cause skin damage. Peel off mask is a cosmetic product that function as a dead skin peel mechanically containing synthetic polymers that are quite safe and effective. Aim: The purpose of this study was to determine the antioxidant effectiveness of the Myrmecodia sp extract. Method: The research used variations in the Myrmecodia sp extract of 2 %, 3%, and 4 %. The evaluation was carried out on the physical properties of the peel off mask and the antioxidant activity test. The antioxidant activity test used the 2,2-Diphenyl-1-picrylhydrazyl method. Statistical tests were carried out on the physical properties of the preparation and IC50 peel off mask using the one-way ANOVA test with a 95% confidence level. Result: Based on this research, it was found that the gel peel-off mask preparation of Myrmecodia sp. extracts has organoleptic of a bit thick, brown and odourless. The value of adhesion is between 10.33-13.04 seconds, pH 6.27-6.75, viscosity 17.00-22.67 dPas, and drying time 17.33-24.33 minutes. The test for the antioxidant activity of IC50 peel off mask at F 2, F 3 and F 4 was 49.4 ppm, 39.41 ppm and 35.25 ppm respectively. Conclusion: The peel-off gel mask of Myrmecodia sp. extracts has the potential as an antioxidant with the strong ability category.

Effect of Variation Conditions of The Extraction Process of Morinda Citrifolia L Leaves Using Ultrasound-Assisted Extraction Method (Uae) Buanasari, Buanasari; Dhamayanti, Sonia Murniana Sri; Suryaningsih, Suryaningsih
Journal of Science and Technology Research for Pharmacy Vol 1 No 1 (2021)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jstrp.v1i1.44348

Background: Morinda citrifolia L. is widely used as traditional medicine for various diseases. The benefits of noni are studied from the seeds, fruit, leaves and root bark. This leaf active compound is rich in flavonoids, so an effective extraction process is needed to extract it. Conventional extraction generally takes a long time and involves a thermal process that can damage the compound, so it requires extraction with the latest methods, one of which is the use of ultrasonic waves. Aim: This study aims to examine the effect of variations in extraction process conditions on present yield, DPPH scavenging activity, flavonoid content and phenol content of Morinda citrifolia L leaves by varying the solids-solvent ratio (1:10, 1:20, 1:30, dan 1:40 g/mL), and extraction temperature (25, 35, 45, and 55ÂºC). Method: The process uses the ultrasonic assisted extraction method with 50 %V ethanol for 60 minutes. Result: The highest yield was obtained in the extraction with a solids-solvent ratio of 1:40 g/mL, at an extraction temperature of 55ÂºC, which was 32.29Â±0.066%. The highest flavonoid content (173.41Â±0.615 mg quercetin equivalent/g extract), phenol content (197.00Â±0.148 mg gallic acid equivalents/g extract) and DPPH scavenging activity (97.65Â±0.912%) was obtained in the extraction with a solids-solvent ratio of 1:30 g/mL, at an extraction temperature of 25ÂºC. The best extract measured antioxidant activity and IC50 values obtained with 23.21 Âµg/mL. Conclusion: The use of the ultrasonic assisted extraction method by selecting the optimal operating conditions greatly increases the amount of active compound uptake required.

Quality Test of Palm Cooking Oil Used Repeatedly Based on Free Fatty Acid Content, Moisture Content, Peroxide Number Dewi, Pevy Syntia; Ulfah, Maria
Journal of Science and Technology Research for Pharmacy Vol 1 No 1 (2021)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jstrp.v1i1.44461

Background: Cooking oil used repeatedly can increase levels of free fatty acids and the number of peroxides in the blood, so that the risk of heart disease and diabetes. Aim: The purpose of the study was to find out the quality of palm cooking oil used repeatedly in tempeh mendoan against organoleptic, moisture content, free fatty acid content and peroxide number content. Method: Samples of palm cooking oil are used to fry tempe mendoan at 105oC for 10 and 60 minutes as much as 1 frying pan per day for 3 days, then the oil is cooled at room temperature and filter the oil so that it does not mix from the rest of the fried tempeh mendoan. Filtered oils are tested for quality with test parameters including organolepty including shape, color, odor, moisture content, peroxide levels, and free fatty acid levels. The data results were compared with sni 01-3741-2002 cooking oil quality standard and each level was analyzed using nonparametric statistics (Kruskall-Wallis) with a confidence level of 95%. Result: Organoleptic test results obtained the form of cooking oil in light yellow to yellow gold at the 10th minute on the 2nd day after 2 frying pans and orange in the 60 minutes after 3 frying pans for 3 days. Moisture content obtained results 2,086Â± 2,142 (10th minute) and 0.369Â± 0.304 (60th minute), free fatty acid content obtained results 0.317Â± 0.044 (10th minute) and 0.337Â± 0.076 (60th minute), the rate of peroxide numbers obtained resulted in 0.281Â± 0.090 (10th minute) and 0.386Â±0.158 (60th minute). Based on statistics tests there are significant differences (<0.05) to the decrease in water content quality, free fatty acid content and peroxide levels in palm oil cooking oil used repeatedly in tempe mendoan.

The Role of Mesenchymal Stem Cell in Controlling Liver Function on Liver Fibrosis Antari, Arini Dewi; Prasetio, Ardi; Nugroho, Baskoro Adi
Journal of Science and Technology Research for Pharmacy Vol 1 No 2 (2021)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jstrp.v1i2.45699

ABSTRACT Background: Liver fibrosis (LF) is the end-stage of liver damage characterized by the increase concentration of SGOT and SGPT. A previous study, revealed that liver transplantation as the most effective therapy for LF has limited availability and remains a major challenge. Mesenchymal stem may control inflammation and regenerate damaged tissue leading to accelerated liver function repair. Aim: The purpose of this study was to determine the optimum doze for MSC administration to recover LF. Method: The research used variations doze of MSCs administration of 1x106 and 2x106. Liver firbosis was induced by twice a week intraperitoneal injection of carbon tetrachloride (CCl4) (Sigma-Aldrich) dissolved with olive oil in 1:1 ratio for eight weeks. The concentration of SGPT SGOT was measured at day 9 post MSC administration. One Way Anova was performed to analyze statictical data. Result: This study showed that there was a significant decrease in SGOT concentration (P < 0,05). P2 group treatment showed the lowest concentration of SGOT among the treatment (Figure B). In the same time, the administration of UC-MSCs reduce SGPT concentration insignificantly (P > 0,05). Conclusion: The transplantation of MSCs ameliorated LF by reducing SGPT and SGOT concentration

Potential of Ginger (Zingiber officinale), as anti-hypercholesterolemia to prevent metabolic syndrome: Systematic Literature Review Melani, Beta; Vikasari, Suci Nar
Journal of Science and Technology Research for Pharmacy Vol 1 No 2 (2021)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jstrp.v1i2.49573

Abstract Background: One of the symptoms of metabolic syndrome is an increase in cholesterol levels in the blood. Ginger (Zingiber officinale) is a herbal plant that has several active compounds that can help improve lipid levels in the blood. Aim: This systematic study was conducted to examine the potential of ginger as an anti-hypercholesterolemia to prevent metabolic syndrome. Methods: The study was conducted on Indonesian-language articles through the Google Scholar Indonesia database. The inclusion criteria used were articles in Indonesian, preclinical and clinical experimental research, and complete articles that were accessible free of charge. Results: The results of the study show that the anti-cholesterol effect of ginger has been proven both preclinically and clinically. To reduce blood cholesterol levels, ginger can be used combined with other herbs. Some of the active ingredients in ginger are phenolic compounds, including gingerols and shogaols. The suspected mechanism of action is to affect the HMG-CoA reductase enzyme by inhibiting the oxidation pathway and as antiinflammation. Conclusion: Ginger has the potential to prevent metabolic syndrome by reducing cholesterol levels in the blood Keywords: Ginger, Zingiber officinale, hypercholesterolemia, metabolic syndrome

The Antibacterial Activity of Acanthus ilicifolius L. n-Hexane Fraction warsinah, warsinah; Wijaya, Triyadi Hendra; Ekowati, Nuraeni
Journal of Science and Technology Research for Pharmacy Vol 1 No 2 (2021)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jstrp.v1i2.49615

Background: Recently, with the high prevalence of diarrhoea caused by bacterial infection, the usage of antibiotics has increased. Antibiotic overuse might lead to several side effects and resistance, suggesting the development of an alternate antibacterial agent. A mangrove plant, Acanthus Illinois, contained triterpenoid, which has antibacterial properties. Aim: This study aimed to evaluate the antibacterial effect of the Acanthus Illinois n-hexane fraction against Escherichia coli and Shigella dysenteriae. Method: The Acanthus Illinois was fraction using n-Hexane, identification of secondary metabolite compound using GC-MS, and evaluation of antibacterial activity against Escherichia coli and Shigella dysenteriae under paper disc methods. This study was designed using a fully randomized design (CRD) with concentrations of fractions 1%, 2%, and 4%. The GC-MS results were compared to the WILEY 9 library and analysed. A prism graph was used to measure the inhibition zone of antibacterial activity. Result: The n-hexane fraction yield is 3.3% and contains sesquiterpene compounds (trans (beta.)-caryophyllene, alpha humulene, naphthalene decahedron-4A-methyl), terpenoid alcohol (3,7,11,15-tetramethyl -2-hexadecane-1-ol), and fatty acids (hexadecanoic acid methyl ester. Acanthus Illinois n-hexane fraction has antibacterial activity against Escherichia coli and Shigella dysenteriae in a dose-dependent manner. Conclusion: The n-hexane fraction of leaves Acanthus ilicifolius contains sesquiterpene, alcohol terpenoids, and fatty, and has antibacterial activity against Escherichia coli and Shigella dysenteriae

Total Phenolic, Total Flavonoid Content and in vitro Sun Protection Factor test of Arabica Coffee Leaves Extract (Coffea arabica L) Fatmawati, Sofia; Sjahid, Landyyun Rahmawan; Utami, Nadiyya Maulida; Kartini, Kartini
Journal of Science and Technology Research for Pharmacy Vol 1 No 2 (2021)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jstrp.v1i2.51374

Background: Arabica coffee leaf (Coffea arabica L.) is a well-known plant by the Indonesian people because it has many benefits apart from being a drink. Coffee leaves are known to have good antioxidant activity. The use of antioxidants in sunscreen preparations can prevent various diseases caused by UV radiation from UV rays’ sunlight. Aim: This study aims to determine the phenol content, the flavonoid content and the value of Sun Protection Factor (SPF) from differences between solvent extracts of arabica coffee leaves (Coffea arabica L.). Method: The extraction method used was maceration with solvents: 70% ethanol, 96% ethanol and methanol. Determination of phenol content was carried out using the Folin ciocalteu method. Determination of flavonoid content was done by the AlCl3 method. SPF value is based on the Mansur equation from absorbance scanning using UV Spectrophotometry. Result: The results obtained were the highest phenol and flavonoid content is in the methanolic extract of arabica coffee namely 62.371 ± 0.47 mgGAE/g and 8.6707 ± 0.04 mgQE/g. In the SPF test, the highest value was obtained from 70% ethanol extract of 5.0593 ± 1.28 at 100 ppm extract concentration with intermediate protection category. Conclusion: Arabica coffee leaves have the potential as an active ingredient in herbal sunscreens

Alpinia galanga Extract Inhibits MCF-7/HER2+ Cells by Inducing Apoptosis Ibrahim, Sugeng
Journal of Science and Technology Research for Pharmacy Vol 1 No 2 (2021)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jstrp.v1i2.51512

Background: Cancer is a disease caused by abnormal growth of body tissue cells. Cancer treatment strategy by induce apoptosis and inhibit proliferation. Aim: This research aims to examine the anticancer effect of Alpinia galanga extract on MCF-7/HER2+ breast cancer cells. Method: The MTT Assay cytotoxic test was carried out to determine the growth inhibitory activity. Apoptotic assay to determine the activity of compounds in apoptosis, as well as through a structural approach utilizing various virtual platforms to monitor its activity. Result: Based on the MTT assay Alpinia galanga extract possessed cytotoxic effect in dose-dependent manner with IC50 value of 330.79 μg/mL. The extract induces apoptosis of MCF-7 cells up to 25.56%. Conclusion: It can be concluded that Alpinia galanga extract demonstrated cytotoxic activity toward MCF-7/HER2+ breast through apoptosis induction. This extract had an opportunity to be developed as a potential anticancer agent to overcome breast cancer diseases.

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
jstrp@mail.unnes.ac.id

Phone
-

Journal Mail Official
jstrp@mail.unnes.ac.id

Editorial Address
D8 Building 2nd Floor, Sekaran, Kec. Gunung Pati, Kota Semarang, Jawa Tengah 50229

Location
Kota semarang,

Jawa tengah

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email jstrp@mail.unnes.ac.id

Phone -

Journal Mail Official jstrp@mail.unnes.ac.id

Editorial Address D8 Building 2nd Floor, Sekaran, Kec. Gunung Pati, Kota Semarang, Jawa Tengah 50229

Location Kota semarang, Jawa tengah INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
jstrp@mail.unnes.ac.id

Phone
-

Journal Mail Official
jstrp@mail.unnes.ac.id

Editorial Address
D8 Building 2nd Floor, Sekaran, Kec. Gunung Pati, Kota Semarang, Jawa Tengah 50229

Location
Kota semarang,

Jawa tengah

INDONESIA