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Edy Kurnianto
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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 14 Documents
 1   2 
Search results for , issue "Vol 43, No 2 (2018): June" : 14 Documents clear
Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle Maharani, D.; Fathoni, A.; Sumadi, S.; Hartatik, T.; Khusnudin, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.87-93

Abstract

MC4R gene is known as an important candidate gene for the growth trait. The purpose of this research was to identify the MC4R gene in Kebumen Ongole grade cattle and examine its association with growth traits. Data of birth weight (BW), weaning weight (WW), birth body length (BBL), birth chest circumference (BCC), birth shoulder height (BSH), weaning body length (WBL), weaning chest circumference(WCC), weaning shoulder height (WSH) and average daily gain (ADG) were collected and used for analysis of MC4R gene. Sixty blood samples were collected for DNA isolation and PCR amplification. The single nucleotide polymorphisms (SNP) g.1133 C>G was used for genotyping by using PCR-RFLP methods. The frequenciy of G allele (0.59) was greater than C allele (0.41). The highest genotype frequencies have been detected in CG heterozygote animals (0.52) followed by GG (0.33) and CC (0.15) in homozygote animals. The results of Pearson ‘s Chi-square test indicated that the population was not deviate (P>0.05) from the Hardy-Weinberg equilibrium (HWE). The SNP g. 1133 C>G of MC4R gene indicated affecting high birth body length with GG genotype (P<0.05). In conclusion, the SNP g. 1133 C>G may can be a marker for birth body length of calf selection.
Survival of Lactobacillus plantarumU40 on the in vitro rumen fermentation quantified with real-time PCR Astuti, W.D.; Widyastuti, Y.; Wina, E.; Suharti, S.; Ridwan, R.; Wiryawan, K.G.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.184-192

Abstract

The objective of this study was to evaluate the survival of L. plantarumU40 quantified with real-time PCR during in vitro rumen fermentation. The experiment was arranged in a randomized block design with 3 treatments and 4 replications. Treatments were control, rumen fermentation inoculated with L. plantarumU40and L. plantarumU40 + glucose solution. Population of L. plantarum U40 was higher at inoculation treatment. After 8 hours incubation, glucose addition tended to decrease L. plantarum U40 population. Control treatment showed lowest population of L. plantarum U40 along in vitro fermentation compared with other treatment. Inoculation of L. plantarumU40 significantly (p<0.05) increased population of LAB until 12 hours incubation compared with control. Control treatment had highest pH at all incubation time. Glucose addition significantly (P<0.05) decreased final rumen pH (24 hours) (6.30), compared with control treatment (6.85). Inoculation of L. plantarum U40 with glucose addition significantly (P<0.05)increased propionic acid, decreased acetic acid and A/P ratio compared with other treatments. Lactobacillus plantarum U40 without glucose addition did not affect propionic acid production significantly. As conclusion, Lactobacillus plantarum U40 can survive in rumen fluid and changes rumen fermentation when glucose is added as carbon source. 
Front-matter (June 2018) Kurnianto, Edy
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.i-v

Abstract

The prevalence of pathogenic bacteria and antimicrobial resistance in milk of Ettawa Grade goat Andriani, A.; Suwito, W.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.140-148

Abstract

Ettawa Grade (PE) are potentially developed goats to produce milk and meat. Milk is food of animal that is rich in nutrients, but it is a perishable food easily contaminated by microorganisms. Contaminated pathogenic bacteria in milk can decrease the quality and has an organoleptic effect on milk, as well as endangers human health. Milk contaminated with bacteria antimicrobial resistance (AMR) in which is resistant to antibiotics, may adversely affect the response to treatment with antibiotics in humans when suffering from infectious diseases and using antibiotics in therapy. In this study Ettawa Grade's samples of fresh milk and other dairy products were taken from some of the goat farms in Yogyakarta Sleman district. The samples were tested for the presence of pathogenic bacteria and for its resistance to several kinds of antibiotics. In this study 35 Ettawa Grade's samples of fresh milk and other dairy products (fresh milk, milk powder, ice cream, and yoghurt) were taken from some of the goat farms in Sleman district-Yogyakarta. The samples were tested for the presence of pathogenic bacteria and for its resistance to several kinds of antibiotics. The result of the prevalence of pathogenic bacteria in goat fresh milk and other dairy products was 15% Escherichia coli and had multi resistance to multiple antibiotics, namely ampicillin, colistin sulphate, cefixime, kanamycin, oxytetracycline, tetracycline and sulfonamide.
Non-genetic factor and genetic parameter analysis for growth traits in Sumba Ongole (SO) cattle Putra, W.P.B.; Agung, P.P.; Said, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.94-106

Abstract

The aim of this study was to evaluate non-genetic factors and genetic parameters of the growth traits in Sumba Ongole (SO) cattle. The growth traits were consisted of birth weight (BW), weaning weight (WW), yearling weight (YW), average daily gain of pre-weaning (ADG1) and post-weaning (ADG2). Data from 143 heads of SO cattle (year 2011 to 2016) which raised at PT KAR were used in this study. Generalized Linear Model (GLM) analysis was performed to evaluate non-genetic effect including sex, year of birth, generation and season. Therefore, to evaluate genetic parameters, the heritability (h2) and genetic correlation (rg ) were performed using Paternal Halfshib Correlation method. The results showed that sex of calf had no significant effect (P>0.05) on BW, WW and YW, but year of birth had significant effect on those traits. The factor of season had significant effect on WW. The estimation of h2 values of growth traits were included high category (h2>0.30) and accurate (h2>SE) on BW (0.66±0.42), WW (0.65±0.44), YW (0.67±0.42), ADG1 (0.68±0.45) and ADG2 (0.70±0.43). The estimation of rg values were included high category (rg>0.50) and accurate (rg>SE) on BW-WW (0.87±0.63); BW-YW (0.95±0.87); ADG1-WW (0.99±0.34); WW-YW (0.98±0.48) and ADG1-YW (0.95±0.51). It was concluded that trait of WW could be used as selection criteria to increase YW trait in SO cattle. 
Back-matter (June 2018) Kurnianto, Edy
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.App.1-App.8

Abstract

Cholesterol Assimilation of Saccharomyces cerevisiae B-18 isolated from gastrointestinal tract of Javanese duck Istiqomah, L.; Anwar, M.; Anggraeni, A.S.; Damayanti, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.149-158

Abstract

This study had a purpose of obtaining potential indigenous yeasts for assimilating cholesterol and assessed the in vitro activity of Bile Salt Hydrolase (BSH) using Chloramphenicol Yeast Glucose (CYG) media supplemented CaCl2 and Taurodeoxycholic Acid (TDCA). Yeasts were collected from the gastrointestinal tract of Indonesian native chicken (Gallus javanicus), Javanese duck (Anas javanicus), and Muscovy duck (Anas moschata). The BSH assay was performed to determine secretion of BSH from yeast strain to conjugate bile salts into cholic acid-free by measuring precipitation zone in a specific medium. The quantitative measurement to assimilate cholesterol in yeast using CYG broth contained soluble cholesterol (500 ppm) and incubated at 30°C for 72 hours. Microplate reader used to analyze cholesterol content in the supernatant. Data were analyzed using Analysis of Variance (ANOVA) with one way completely randomized. Precipitation zone found among isolates did not significantly different (P>0.05). Isolate B-18 from Javanese duck performed the highest percentage of assimilating cholesterol with the value of 51.83% and identified as S. cerevisiae. This isolate was closely related to S. cerevisiae mt 21s (accession number X00149.1)based on phylogenetic tree analysis. It could be concluded that S. cerevisiae B-18was potential for assimilating cholesterol in vitro.
Effect of binder mineral in batchery waste based feed pellet on its proximate component and energy values Sulistiyanto, B.; Utama, C.S.; Sumarsih, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.107-114

Abstract

The Experiment was conducted to study effects of binder mineral addition to the hatchery waste based pelletprocessingon its proximate component and energy value. The study may be beneficial to emerge a new feed resource for local farm businesses. The hatchery wastes those comprised of 30% of egg shells, 60% of un-hatched eggs, and 10% of culled DOC were blended, and mixed with 10% of cassava cake meal(W/W), then mixed well with mineral adsorbent (bentonites or zeolites) at 0, 1, 2, 3, 4 and 5% (W/W). The mixture was conditioned at 80 ºC for 15 min, and extruded to make a pellet with 6 mm of diameter and 30 mm in length, and dried into warm air flow dryer. The moisture, crude protein (CP), ash, ether extract (EE), crude fiber (CF), apparent metabolizable energy (AME), true metabolizable energy (TME), metabolizability(MET) and nitrogen retention (NR) were observed in each pellet. Result of the experiment showed that moisture of pellet was decreased (P<0.05) by zeolites or bentonites addition. Even though the effect ofbinder mineral addition gave different patterns, concentrations of ash, crude protein, ether extract and crude fiber of pellet product were increased by increasing level of mineral adsorbent (P<0.05). Nutrient availability as was represented by AME, TME, MET, and NR were increased by zeolites and bentonites additions (P<0.05). In conclusion, supplementations of bentonites and zeolites in the hatchery waste based pelletcould improve its proximate component concentration and nutrient availability.
The improvement of functional food in yogurt enriched with purple sweet potato (Ipomea batatas var. Ayamurasaki) Afiati, F.; Priadi, G.; Setiyoningrum, F.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.159-168

Abstract

he research was conducted to examine the use of purple sweet potato (Ipomoea batatas var. Ayamurasaki) to improve functional food of yogurt. This experiment has 2 factors of treatments. The first factor (i) was concentration of skim milk, i.e. 0%, 3% and 6%; the second factor (ii) was concentration of purple sweet potato, i.e. 0%, 2% and 4% of purple sweet potato. The parameters observed were viability of lactic acid bacteria (LAB), pH, moisture content, protein, fat, carbohydrate, ash and crude fiber and organoleptic. The results showed that there was no interaction between the concentration of skim-milk and the purple sweet potato on total LAB, moisture content and crude fiber. However, the interaction occurred on the levels of fat, protein, carbohydrate and ash. The organoleptic test results that yogurt enriched by 2% purple sweet potato without addition of skim was more preferable by panelist with a value of 3.65.
The potential of Bacillus strains isolated from the rumen content of dairy cows as natural antibacterial and antioxidant agents for broilers Sugiharto, S.; Yudiarti, T.; Isroli, I.; Widiastuti, E.; Wahyuni, H.I.; Suprijatna, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.115-123

Abstract

The study was conducted to investigate the in vitro antibacterial activity and antioxidant-enhancing effect of Bacillus strains isolated from the rumen content of dairy cows. The antibacterial activity of Bacillus strains was assessed against Escherichia colibased on the agar-well diffusion method. To assess the antioxidant-enhancing effect, the Bacillusstrains was mixed (prepared as multi-strains) and used to ferment herbal medicine waste (HMW). The effect was compared with that of commercial probiotic Bacillus subtilis and non fermented HWM. Results showed that each Bacillus strain was able to inhibit the growth of E. coli. Fermentation with mixed Bacillus strains tended (P=0.07) to enhance the 2,2-diphenylpicrylhydrazyl (DPPH) radicals scavenging activity and total phenolics content of HMW. In contrast, fermentation with commercial Bacillus subtilis tended (P=0.07) to decrease DPPH radical scavenging activity and total phenol of HMW. Fermentation with both Bacillus preparations increased (P<0.05) the contents of crude protein and ash in HMW, while crude fat increased  (P<0.05) in HMW fermented with Bacillus subtilis. Fermentation either with Bacillus subtilis or mixed Bacillus strains increased (P<0.05) the fiber content of HMW. In conclusion, Bacillus strains isolated from the rumen content of dairy cows showed antibacterial activity and antioxidant-enhancing effect, therefore, they are potential as feed additive to substitute synthetic antibiotics and antioxidants for broilers raised under tropical conditions.

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