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Research Journal of Life Science

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Published by Universitas Brawijaya

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Welcome to the Research Journal of Life Science, published by the Institute of Research and Community Service UB. For further information please contact our editorial office.

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Articles 187 Documents

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The Relationship Among Four Pillars of Diabetes Mellitus Management with Blood Glucose Levels and Nutritional Status in Middle-Aged Diabetic Adults Kanthi Permaningtyas Tritisari; Ayuningtyas Dian Ariestiningsih; Dian Handayani; Inggita Kusumastuty
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Controlling the blood sugar level of DM patients is an approach to suppress morbidity and mortality. This study aims to know the relationship of four pillars of DM management with blood glucose levels and nutritional status in patients with type 2 DM in East Java. This study is a cross-sectional study with a sample of middle-aged type 2 DM outpatients in 4 cities in East Java, Indonesia. Four pillars of management include education profile, food planning, physical exercise, and drug adherence. The statistical analysis used was Partial Least Square. The results of the study show that four pillars were not associated with fasting blood glucose (FBG) level and 2-hour PPG in the total number of respondents (p>0.005). Interestingly, dietary intake links to 2-Hour PPG in 40-50 years age group (p=0.000) and was associated with body mass index in the total number of respondents (p=0.000). Furthermore, education profile and medication also related to FBG in 40-50 years age group (p=0.000, p=0,003). In conclusion, the success of the four pillars in controlling DM is affected by age. In 40-50 years age group, there is an influence of education profile and drug adherence on the level of FBG.

Biofilter Application Using Seaweed (Gracillaria verucosa) to Increase Production of Vannameii Shrimp in Traditional Pond District Bangil-Pasuruan Sri Andayani; Rani Yuwanita; Nailul Izzah
Research Journal of Life Science Vol 3, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The aim of this research to know how to operate and develop the Vannamei production by using biofiter technology of seaweed to the farmer, keeper, young people and leader of the society. The results showed that the Integration of Gracilaria verucosa as a biofilter agent in aquaculture pond can increase the production of Vanname shrimp. The results were obtained 5 tons/ha of seaweed from the initial density of seaweed 1 ton/ha after 3 months of culture with daily growth rate of 4.18%/day. Vannamei shrimp growth of 0.5 grams to 16.7 grams/fish, shrimp production from 1 ton/ha to 3,5 tons/ha. Based on the economic analysis showed gains with the value of R/C ratio of 1.83 and a profitability of 80.08%. Biofilter methods is more likely, given the application is very simple, high adaptability, easy maintenance, and economic value. Socialization and counseling about the application of environmental friendly biofilter system using G. verucosa needs to be carried out at farmer pond.

Optimization of Plant Growth and Yield Through Innovation of the Verticultural Materials and Media Sitawati Sitawati; Agus Suryanto; Euis Elih Nurlaelih
Research Journal of Life Science Vol 3, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Vertikultur is one of effort to agricultural of narrow as urban farming. Efforts to obtain plant container and plant medium on vegetables pakcoy to compare the productivity model of vertical planting and horizontally with the application of fertilizer on strawberry plants optimally. The first study aimed to determine the combination of growing medium and materials vertikultur which efficient and effective for pakchoy growth in vertikultur, then continued a second study that application of a combination of materials vertikultur and fertilizer N, P and K on the strawberry plants at planting vertical and horizontal models. Combination treatment is the first study consisted of the first factor is the material vertikultur form of carpet, gunny sacks and plastic, while the second factor is the growing media consisting of soil, humus, rice husk and cocopeat using a randomized design factorial with three replications conducted in Gresik, East Java. In the research into two, the first factor is a model of planting both horizontally and vertically, while the factor two is a dose of NPK (0%, 25%, 50%, 75% and 100%) on strawberry plants by using Design Grid Divided by 3 replications implemented in Batu, East Java. The results showed that the material vertikultur carpets and plastic with humus media have fresh weight pachoy same plant with soil media. Average penamaman with vertical and horizontal models have the same weight of strawberries where urea 3.75 g / tan, SP36 5g / tan and KCl 5 g / tan increase the yield per plant.

Cuttlefish (Sepia Spp) Identification and Biological Analysis of A Dominant Cuttlefish Species Landed in Muncar, Banyuwangi Regency, East Java. Daduk Setyohadi; Sunardi Sunardi; Nurul Mukhlis; Citra Nilam Cahya
Research Journal of Life Science Vol 3, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Identification and biological analysis of Cuttlefish (Sepia spp) this could be the basis of information for cuttlefish resource management for sustainability. The aims of this study were to determine the species composition of cuttlefish, determine similarity in morphology among cuttlefish species, and length-weight relationship of dominant species cuttlefish landed in Muncar, Banyuwangi, East Java. The analytical method used is species composition analyze, Hierarchical clustering analysis to determine similarity among cuttlefish species and length-weight relationship analysis of dominant cuttlefish species with the following equation W = aLb. There are three species of cuttlefish were landed in Muncar, Banyuwangi, Sepia pharaonis with 373 individuals (92.6%), Sepia latimanus 19 individuals (4.7%), and Sepia esculenta 11 individuals (2.7%). The results of length-weight relationship analysis cuttlefish dominant species, Sepia pharaonis, the b values obtained in the total sample, males and females respectively are 2.55, 2.56, and 2.57. It shows that the growth properties of S. pharaonis is negative allometric. It means the population has thin body shape where the increase in length faster than weight gain.

Identifying and assessing Spice and herbs resources for tourism attractions: A case study from Banyuwangi, East Java Luchman Hakim; Soemarno Soemarno; Bagyo Yanuwiyadi
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The aims of the study was to analyze the potentiality of spices and herbs in agrofotestry garden in Banyuwangi, Indonesia, for further rural tourism development. Fields study was set up at villages in Banyuwangi. Floristic survey, ethnobitanical studies and focus group discussion were performed to evaluate the value of spices and herbs in agroforestry gardens an its potentiality to develop as tourism product. This study found that at least 60 species of spice and herbs with its special value, in which these species has opportunities to developed as tourism attraction in agroforestry garden. There are something to see, something to buy and something to do has been described by group discussion participant. It is raging from farm tourism product, culinary tourism product and heath-wellness tourism product.

Immunomodulation Effects of Bryophyllum Pinnatum on Pregnant Pristane-Induced Lupus Mice Model Nurdiana Nurdiana; Elvira Sari Dewi; Yuni Sari; Rahmawati Wahyuni; Aminah Maya; Nursari Abdul Syukur; Mirza Zaka Pratama; Wisnu Barlianto; Umi Kalsum; Kusworini Handono
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Objective: To determine the effect of Bryophyllum pinnatum treatment in modulating immune response and the pregnancy outcomes of pregnant pristane-induced lupus mice model.Methods: Sixteen Balb/c mice were intraperitoneally injected with single 0.5 cc pristane to induce lupus manifestations. After 12 weeks of injection, mice were mated and considered as gestational day 0 (GD0). Mice were divided into 4 groups based on the dosages of Bryophyllum pinnatum: control (no treatment), B1 (10.5 mg/kg), B2 (21 mg/kg), and B3 (42 mg/kg). The treatment was given orally every day started from GD9 until 9 days. At the end of the study, blood pressure and fetal size were measured. Serum anti-dsDNA and urine albumin levels were measured by ELISA. Spleen T helper (Th) and mature B cells percentages were measured by flow cytometry.Results: Administration of Bryophyllum pinnatum reduced the percentages of Th1 (p=0.006), Th2 (p=0.005), Th17 (p=0.000), and mature B cells (p=0.007) in dose-dependent manner. B1 and B2 had significantly lower of systolic blood pressure compared to control (p=0.026 and p=0.022 respectively). Significantly lower of anti-dsDNA levels were found in B1 group compared to control (p=0.014). However, no significantly different of urine albumin levels were found between groups. Bryophyllum pinnatum also significantly increased the fetus body weight in dose-dependent manner (p=0.000).Conclusion: Treatment of Bryophyllum pinnatum could improve the pregnancy outcome and modulate the immune response in pregnant pristane-induced lupus mice. Therefore, Bryophyllum pinnatum is a potential herb which can be developed as an immunosuppressive agent in the future.

Survey of Hawksbill Turtle (Eretmochelys imbricate) Health Condition in Terms of Parasites and Microbes in Alas Purwo National Park, Indonesia Qurrota A'yunin; Happy Nursyam; Sri Andayani; Maftuch Maftuch; Lukluk Intan Maftuchah
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Indonesian waters have six types of turtles that can live, spawn and breed. Sea turtle conservation becomes an important and urgent program to be done in order to protect and save sea turtle population in Indonesia. One of the factors that most affect the turtle population is the cause of degradation of pathogenic factors. Alas Purwo National Park, East Java, there is some communities that have activities turtle conservation. Conservation is done by securing and protecting turtle eggs. Turtle eggs that have hatched are released into the sea once it is ready. This study aims was to determine the type of bacteria and fungi that infect hatchlings and environmental factors that influence. This research is descriptive method to Hawksbill turtle (Eretmochelys imbricate) is by way of random sampling. Sampling of microbes in sea turtle was conducted using cotton swab method and then microbes was cultured and indentified in laboratory. The results showed The kind of parasites and microbes which were indentified in hatching and adult Hawksbill sea turtles were fungus with genus Aspergillus sp., Geotrichum sp., Fusarium sp., and Gliocladium sp. ; bacteria are Pseudomonas aeruginosa and Enterobacter cloaceae; and parasite is Chelonibia testudinaria barnacles. The parameter average value of water in pond indicated 28.1 – 29.2°C for temperature, 32 - 34 ‰ for salinity, 7.78 – 8.2 for pH, and 3.86 – 4.21 mg/L for DO.

Development of an Experimental Model of Metabolic Syndrome in Sprague Dawley Rat Mohammad Saifur Rohman; Mifetika Lukitasari; Dwi Adi Nugroho; Widodo Nashi; Nur Ida Panca Nugraheini; Teguh Wahyu Sardjono
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The study was designed to establish an experimental models of metabolic syndrome that adequately mimic metabolic syndrome criteria as determined by NCEP ATP III.Eighteen Male Sprague dawley rats, 2 - 3 months old were used in the study. Combination of high fat and high sucrose (HFHS) diet for eight weeks and streptozotocin (STZ) injection in the second week was administered to induce metabolic syndrome. The body weight and biochemical parameters (blood glucose, triglyceride, HDL-cholesterol) were measured every 2 weeks. The rats with blood glucose (>126mg/dL), triglyceride (TG) (>150mg/dL), high systolic blood pressure (≥140 mmHg), and reduced HDL levels (<40 mg/dL) confirmed presence NCEP-ATP III criteria of metabolic syndrome. The adiponectin level was analyzed by ELISA methods.Fasting blood glucose, triglyceride, and systolic blood pressure increased significantly (p<0.05) in HFHS group compared to that of NC group. Moreover, after 8 week a significant lower HDL level was observed in HFHS group compared to that of NC group. In addition, HFHS group showed a significantly lower adiponectin level compared to that of NC group.The combination of low doses of STZ (30mg/kg) and HFHS administration for 8 weeks could induce metabolic syndrome mimicking human criteria of metabolic syndrome.

Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates Nur Maulida Safitri; Endang Yuli Herawati; Jue Liang Hsu
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The aim of this study is to isolate the antioxidative peptide from Spirulina platensis. Peptide was obtained by proteolytic digestion, ultrafiltration, fractionation by RP-HPLC, identified by LC-MS/MS—MASCOT Distiller and measured its antioxidant activity by DPPH (2.2-Diphenyl-1-picrylhydrazyl) assay. Results showed that thermolysin was the most effective enzyme to digest this algae. The active peptide Phe-Ser-Glu-Ser-Ser-Ala-Pro-Glu-Gln-His-Tyr (m/z 1281.51) was identified and synthetized, which exhibited 45.98 ± 1.7% at concentration 128.15 µg/mL. Therefore, S. platensis is indicated as a potential therapeutic source for combating oxidative stress.

The Effect of Different Seasons and Land Types Towards The Performance of Bali Cattle Production in Southeast Sulawesi La Wangi; Woro Busono; Moch Nasich
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The Bali Cattle in Southeast Sulawesi its productivity is influenced by the seasonal environmental conditions. The objective of this study was to analyze the performance production of cows Bali Cattle and its calves that were born on different season (rainy season and dry season) as well as on different types of land. The research methods applied were survey and observation method. The variables being observed follows: body weight (BWT), daily body weight gain (PBBH), body condition score (BCS) and birth weight (BW). Data were analyzed by using the Analysis of Variance (ANOVA) of nested design. The result of the research indicated that the average of body weight, daily body weight gain, BCS and birth weight was higher during the rainy season compared to the dry season. The average of body weight, daily body weight gain, and BCS was higher on paddy field. On the contrary, the birth weight on dry area was higher than it was on wetlands. The average of male cattle birth weight was higher than the average of female cattle. It could be concluded that the cow’s working performance production was affected by the feed consumption and environments (season and the type of land). On the other hand, calf birth weight was affected by sexes, in addition it was also related to cow’s feed intake on late pregnancy phase.

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