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International Journal of Biosciences and Biotechnology
Published by Universitas Udayana
ISSN : 23033371     EISSN : 26559994     DOI : -
Core Subject : Science, Social,
Biochemistry, Genetics & Molecular Biology Environmental Science
International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences
Arjuna Subject : -
Articles 6 Documents
 1 
Search results for , issue "Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY" : 6 Documents clear
KCl FERTILIZER AND GOAT Urine EFFECT ON PRODUCTION OF JAPANESE CUCUMBER PLANTS (Cucumis sativus var. Japonese) Efrida Lubis; Aisar Novita; Khoirul Bahri; Seca Gandaseca
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p03

Abstract

The research was carried out from June to completion, at the Deli Tobacco Research Institute (BPTD) Sampali PTPN II. This study aims to determine the effect of KCl fertilizer and goat urine on the growth and yield of Japanese cucumbers. This study used a factorial randomized block design (RAK) with 2 factors. The first factor is KCl with 4 levels, namely S0 (Control), S1 (10 g/plant), S2 (20 g/plant), S3 (30 g/plant) the second factor is Goat Urine with 4 levels, namely K0 (Control), K1 (200 ml/plant), K2 (400 ml/plant) and K3 (600 ml/plant). Parameters measured were vine length, leaf number, leaf area, number of fruit per plant, fruit length, number of fruit per plot, fruit weight per plant and fruit weight per plot. The results showed that KCl fertilizer had an effect on the number of fruits per plant and the number of fruits per plot but the administration of goat urine had no effect on all parameters. The interaction of KCl fertilizer and goat urine did not affect all parameters.
THE EFFECTIVENESS OF RHIZOBACTERIA AS BIOPROTECTANTS TO MITIGATE FUSARIUM WILT DISEASE AND AS BIOSTIMULANTS TO IMPROVE THE GROWTH OF CHILI (Capsicum annuum) Khamdan Khalimi; Gusti Ngurah Alit Susanta Wirya; I Putu Wirya Suputra; Ni Luh Putu Citra Innosensia
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p04

Abstract

The aims of this study were to examine the potential of Rhizobacteria as bioprotectants and biostimulants on chili. The potential as bioprotectants was tested by the ability of these Rhizobacteria to inhibit the growth of the pathogenic fungus Fusarium oxysporum f.sp. capsici in vitro. Moreover, as biostimulants was tested by the ability of these rhizobacteria to produce IAA compounds and their ability to increase the plant growth parameter of chili. Four rhizobacteria were challenged for antagonistic activity against F. oxysporum f.sp. capsici. Chili seeds in this experiment were soaked in four different Rhizobacteria suspension for 15 minutes. For control, seeds were soaked with sterile water instead of Rhizobacteria suspension. Results of this experiment showed that four isolates of Rhizobacteria showed strong inhibitory activity against F. oxysporum f.sp. capsici on PDA medium. Percentage of inhibitory activity varied from 77,33% to 89,79%. The application of four isolates recorded significantly increased the plant growth parameters of chili. The plant height, number of leaves, leaf area, root length, shoot dry mass, root dry mass, and chlorophyll content on treated plants significantly higher than those of untreated control plants according to the Duncan’s multiple range test (P<0.05%). These results indicate that the tested Rhizobacteria can be used as bioprotectants because they can inhibit the growth of F. oxysporum f.sp. capsici and simultaneously as a biostimulant for the ability to produce IAA compounds and stimulate the growth of chili.
RISK ASSESSMENT OF NITRAT CONTAMINATED IN GROUNDWATER IN GIAO THUY DISTRICT, NAMDINH PROVINCE, VIETNAM Ha Thi Thu Ly; An The An; Ha Thi Nguyen; Bang Nguyen Tran
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p05

Abstract

Environmental pollution due to agricultural production activities, and the use of fertilizers and pesticides is a matter of great concern due to its harmful effects on the soil environment, water environment, and ecosystems. In this study, the risk of nitrate contamination in shallow groundwater related to agricultural land was evaluated using DRATIC and IPNOA models with 66 soil samples and 132 groundwater samples. The results showed that hazard was found at very low with HI values ranging from 3.5-6.06. The RI of nitrate-contaminated groundwater from agricultural activities is in the range of 539 - 1058. The assessment results show that the vulnerability index has a significant impact on the RI index, and areas with a high vulnerability index have a higher risk of groundwater pollution. The NO3- concentration seems to be stable, ranging from 0.02 to 0.11 mg/l, and all meet with the QCVN 09:2018/BNTMT for groundwater quality. No clear correlation between vulnerability factor and risk factor with NO3- concentration in groundwater.
The impact of bagworm (Metisa plana) attack on the production of oil palm (Elaeis guineensis Jacq.) at Tanjung Beringin Plantation PT. Langkat Nusantara Kepong Arie Hapsani Hasan Basri; Stephanie Angelica Girsang; Aisar Novita
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p01

Abstract

The impact of bagworm (Metisa plana) attack on the production of oil palm (Elaeis guineensis Jacq.) at Tanjung Beringin Plantation PT. Langkat Nusantara Kepong. This study aims to assess the extent of attack by bagworms (Metisa plana) and the impact of bagworm attacks (Metisa plana) on oil palm production in Tanjung Beringin Plantation PT. Langkat Nusantara Kepong. The study method used was descriptive statistics by collecting data from the estate office and direct interviews. The study was carried out at Afdeling III Tanjung Beringin Plantation, PT. Langkat Nusantara Kepong. The assessment was carried out from February to July 2022. The statistical analysis method used to determine the impact of bagworm (Metisa plana) attacks on oil palm production was using tables, graphs and Descriptive Statistics analysis. The results of tables, graphs and Descriptive Statistics analysis show that the attack area of the bagworm (Metisa plana) in 2019-2021 has decreased by 66.62% and the attack of the bagworm (Metisa plana) has an impact on oil palm production. The wider the pest attack, the smaller the production. Pest attacks in 2019 of 680 Ha experienced a decline in production in 2020 by 2.33% and pest attacks in 2020 of 374 Ha experienced an increase in production in 2021 by 11.60%.
PHYTOCHEMICAL ANALYSIS OF Mucuna pruriens L. LEAVES EXTRACT AND ITS INHIBITION TEST AGAINST Curvularia lunata (WAKK). BOED. THAT CAUSED LETTUCE LEAF SPOT I Made Ary Putra Nata; I Ketut Suada; Trisna Agung Phabiola
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p06

Abstract

Curvularia lunata is a pathogenic fungi that attack various plant species including lettuce. Its control needs to be carried out in an environmentally friendly manner, namely using botanical fungicides. M. pruriens is a legume plant that is widespread in Indonesia that has a potential as a botanical fungicide, beside as a traditional medicine. The purpose of this study was to study the chemical content of M. pruriens leaves and its potency to inhibit the growth of C. lunata. The determination of the compounds was carried out by Gas Chromatography-Mass Spectrophotometry and the inhibition test was carried out using diffusion well, and agar dillution method. The result showed that the crude extract of M. pruriens leaves contained ethyl isothiocyanate that act as an antifungal with AUC of 20,78%. The MIC of the extract was 0,9%. The extract at 5% concentration was capable to inhibit C. lunata growth, mycelium mass, and conidia production respectively 38,33%; 67,14%; 99,88% compared to control.
Callus Induction In Leucaena (Leucaena leucocephala (Lam.) de Wit) As An Effort To Provide Target Transformation Through Agrobacterium tumefaciens Ni Nyoman Nila Arieswari; Ida Ayu Astarini; Junita Hardini; Austin Ryan Garrido; Debora Margareth; Jennifer Crismonika; Sebastian S. Cocioba
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p02

Abstract

Leucaena is a plant that produces biomass productivity in the form of hardwood for fuel with low humidity and high calorific value. However, Leucaena is also classified as an invasive plant which can cause the urgency of native plant species and ecosystems in Indonesia. Therefore, the formation of sterile Leucaena needs to be done, one of which is through genetic transformation using Agrobacterium tumefaciens. Callus is used as a target for transformants in the genetic transformation process, so it is necessary to use appropriate media and PGR. This study aimed to determine the type of media and the concentration of 2,4-D on callus induction. This research is an experimental study with a completely randomized design (CRD) method with two factors. The first factor is the type of media (MS and WPM) and the second factor is the concentration of 2,4-D (0; 0.25; 0.50; 0.75; 1.00; 1.25 and 1.50 mgL-1 ). Each treatment was repeated three times so that 42 experimental units were obtained. Parameters observed were callus initiation, callus fresh weight (gram), callus texture and color. Quantitative data is analyzed by analysis of variance (ANOVA). The results showed that the use of media had a significant effect (P<0.05) on callus fresh weight. The use of 2,4- D concentration had a significant effect (P>0.05) on callus texture. The use of WPM media resulted in the fastest callus emergence time (6.67±0.57), the best callus texture (crumb callus type 2) and the best callus color (green). Meanwhile, the highest fresh weight (2,48±0.83) was in the use of MS media. The fastest callus emergence time occurred in the control (without the addition of 2,4-D) (7.33±0.57 and 6.67±0.57), the highest average fresh callus weight (2,48±0.83 and 2.35±0.32) occurred in the treatment with the addition of 1.00 mgL-1 2,4-D with a crumb callus texture of type 2 and callus green color only appeared in the treatment with a concentration of 0.25 mgL-1.

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