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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 17 Documents

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Search results for , issue " Vol 10, No 4 (2011)" : 17 Documents clear

KERAGAMAN GENETIKA POPULASI PELAHLAR (Dipterocarpus littoralis (Bl.) Kurz) DIPULAU NUSAKAMBANGAN BERDASARKAN PROFIL ENHANCED RANDOM AMPLIFIED POLYMORPHIC DNA Yulita, Kusumadewi Sri; Partomihardjo, Tukirin
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Dipterocarpus littoralis is a commercial timber species endemic to Nusakambangan island. Their existence is under severe threat due to habitat conversion and illegal logging. This study aimed to assess genetic diversity of this species within and among populations using E-RAPD. Three arbitrary RAPD primers were modified by addition of two nucleotides at their 5 termini.E-RAPD profiles were obtained by performing PCR amplification using the modified primers. Forty-five putative loci of E-RAPD were scored and analysed using POPGENE software. Some of the RAPD bands (OPA 9E-200 and 850 bp) were distinctively found for population 2, thus it served as population diagnostic marker for this population. Genetic diversity within population (0.1540) was higher than that of among populations (0.0418). Genetic differentiation (Gst) indicated that 21.35% of total genetic diversity in D. littoralis was attributed to the differences among populations. The highest genetic diversity was found in population 2(He:0.1923; 1:0.3158), while the lowest genetic variation was observed in population 1 (He: 0.0828; 1: 0.1209). Total genetic diversity for all population (Ht) was 0.1958 with an average value of genetic diversity within populations (Hs) was 0.1540. This fact suggested high level of genetic diversity found on these relic populations.

PENGARUH PERENDAMAN BENIH DALAM CaO DAN PEMUPUKAN P DAN K TERHADAP PENGENDALIAN KERACUNAN BESI PADA TANAMAN PADI DI LAHAN SULFAT MASAM POTENSIAL Susilawati, Ani; Khairullah, Izhar
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Tidal land large enough sour sulfate and the potential for agricultural expansion. Obstacles encountered primarily stress iron poisoning. It has the potential to cause a decline in rice yield research aims to study the effect of the influence of seed treatment and fertilizer P and K to control iron toxicity in acidic sulfate potential land. This research was carried out in KP Belandean on MK 2007. Randomized block design with 3 replications. Rice varieties used were Batanghari, planted on plot measuring 4 mx 9 m with a spacing of 20 cm x 20 cm. Package combined treatment of seeds and fertilizer P and K: 1. (25-90-75), 2. (50-90-75), 3. (75-90-75), 4. (100-90-75), 5. (125-90-75), 6. (75-30-75), 7. (75-60-75), 8. (75-120-75), 9. (75-150-75), 10. (75-90-25), 11. (75-90-50), 12. (75-90-100), 13. (75-90-125), 14. (0-90-75), 15. (0-0-0) kg / ha% CaO-kg / ha P2O5-K2O. The result showed that by giving a dose of phosphate fertilizer 90 kg/ha P2O5 and potassium at a dose of 100-125 kg/ha K2O CaO combined with the provision of 75 % of the weight of the seed, is the combination to control iron poisoning.

DIVERSITAS DAN HILANGNYA JENIS-JENIS IKAN DISUNGAI CILIWUNG DAN SUNGAI CISADANE Hadiaty, Renny Kurnia
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The fish research in Indonesian waters has been begun since 16 century ago. Most of the research collected fish around Batavia.Many new species was described and the type specimens deposited at the museums in Europe or America.The study of fish diversity and the lost of fish species was conducted at River Ciliwung and R. Cisadane in 2009. The aim of this study is to describe the recent fish diversity in both river drainages, then make a comparison with the number of species recorded based on the references data.The differences between the two datas show the number of species lost. The result of the study showed a total of 20 species of 13 families were collected in R. Ciliwung, while from the references data there were about 187 species, so the fish diversity lost in the Ciliwung river about 92.5%. The fish research in R. Cisadane were collected 39 fish species of 24 families. There were 135 species of 38 families of the references data, so the fish diversity lost in R. Cisadane is about 75.6%.

KARAKTERISASI GENETIK IKAN KELABAU (Osteochilus kelabau) DARI BERBAGAI LOKASI DI KALIMANTAN BARAT MENGGUNAKAN METODE RAPD (Random Amplified Polymorphism DNA) Kusmini, Irin Iriana; Gustiano, Rudy; Mulyasari, Mulyasari
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Kelabau fish (Osteochilus sp.) is an endemic fish to Kalimantan inland waters that is potential to be developed. The aim of this research was to characterize Kelabau fish and to study the data base (genetic character) of kelabau as well as its relationship. The result showed that the highest polymorphism and heterozigosity was on Kelabau Sintang and the lowest was on Kelabau Kapuas Hulu. The closest genetic distance value were between Kelabau Pontianak-Kapuas Hulu (0.5351) and the furthest were between Kelabau Pontianak-Sintang (0.6852).

KERAGAAN HIBRIDAINTRA SPESIFIK DARIEMPAT STRAIN IKAN NILA (Oreochromis niloticus) DIKARAMBA JARING APUNG, DANAU LIDO, BOGOR Mahardhika, Prana; Gustiano, Rudhy; Soelistyowati, Dinar Tri; Ath-thar, MH Fariduddin
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The objectives of the study was to evaluate hybrid performance of sixteen reciprocal cross resulted from three strains (Red NIFI,Nirwana, BEST) and one population (Lido Lake population) of nile tilapi. The study was conducted in experimental floating netcages in Lido Lake. Fish of 25 days old ranged 0,4 Â± 0.07 g were used in the experiment. Fish were reared in floating net cages sized 2x1x1 m for each cross with the density of 50 fish/cage for eight weeks (56 days). Feed were given 10% of body weight with frequency three times a day. The results showed that the highest average larvae production was 1216 from Nirwana female crossed with four different male. Growth rate of hybrids were higher than the parents (P < 0.05) and the highest was .ed NIFI x c? BEST (6,440Â±0,0712% ).The highest survival rate was $ Red Lido x S BEST hybrid (77.0 Â±2,78%")). For heterosis, Specific Growth Rate (SGR) was 5.8188% on hybrid between Nirwana and Red Lido. While for survival rate was 15.5888% on Nirwana x BEST hybrid. Crossing between fenotipe of light color background (red) and dark color background (black) showed that red color was dominant to the black one. Pleiotric effect of color gen was detected on the SGR and survival rate.

PENGARUH BAP DAN NAA TERHADAP INDUKSI KALUS DAN KANDUNGAN ARTEMISININ DARI Artemisia annua L. Purnamaningsih, Ragapadmi; Ashrina, Misky
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Malaria is a global health problem that threatens 300-500 million people and kills more than one million people annually.Artemisinin, a sesquiterpen secondary plant metabolite extracted from Artemisia annua L., is a promising and potent antimalarial drug which has a remarkable activity against chloroquine resistant to Plasmodium falciparum. To counter the present low content(0.01-0.5%) of artemisinin in A. annua L.is a limitation to commercial production of the drug and uneconomical chemical synthesis. A research was conducted to induce callus production by using Murashige-Skoog (MS) medium added with NAA (0, 0.5 and 1 mg/1) and BAP (0, 0.5 dan 1 mg/1) and also to produce artemisinin from the calli. Complete Randomized Design was used in the research. Callus cultures were induced from leaf explants of A. annua. The research reports succesful approach for production of artemisinin by callus cultures of A. Annua. Medium formulation of MS basal media added with plant growth regulators BAP 0.5 mg/1 and NAA 0.5 mg/1 give the best result for callus induction than others, with callus fresh weight 844,4 mg, artemisinin content 0.73%, dry weight 216.6 mg and total weight of artemisinin 1.58 mg.

KEONG HAMA Pomacea DI INDONESIA: KARAKTER MORFOLOGI DAN SEBARANNYA(MOLLUSCA, GASTROPODA: AMPULLARIIDAE) Isnaningsih, Nur Rohmatin; Marwoto, Ristiyanti M
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.761

The golden apple snail Pomacea is an invasive species not only in Indonesia but mostly in Southeast Asia. The snail caused serious damaged on more than thousands hectares of rice-fields. The study was based on the specimens deposited at the Museum Zoologicum Bogoriense (MZB)aimed to evaluate the occurrence of Pomacea and its distribution in Indonesia.Based on the shell characters there are four species recognized which are P. canaliculata, P.insularum, P. scalaris, P.paludosa that found from many places, and noted that P. canaliculata has wider distribution from Sumatra to Papua. The description of each species was presented at this paper as well as the map of the occurrence of Pomacea in Indonesia.

EVALUASI KONDISI HUTAN BERDASARKAN KERAGAMAN KUMBANG SUNGUT PANJANG (COLEOPTERA, CERAMBYCIDAE) 1 DI KAWASAN GUNUNG SLAMET Noerdjito, Woro Anggraitoningsih
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Evaluation of forest conditions using longhorn beetles (Coleoptera: Cerambycidae) fauna have been carried out at 3 parts of Mt. Slamet which are, northern part through Guci, southern part through Kalipagu, Baturraden and easthern part through Bambangan. In northern parts, research activities were conducted at about 1200-2500m above sea level (m.asl.), in southern part, research activities was conducted at about 700-1000m m.asl and in eastern part research activities was conducted at about 1500-2400m asl.Out of the 37 species of longhorn beetles were collected during this research, dominated by Sybra fuscotriangularis (55 specimens). The longhorn beetles collected in southern part, indicated lowest individual number (36 spesimen) but indicating hightest species number. In the contrary, the highest number of spesimens collected but lowest species number (10 species) was collected in the eastern part, while in northern part, it was collected 86 individu (17 species).The species compositions and the number of individuals of longhorn beetles collected from each parts (northern, southern and easthern part) were different with specific groups. Longhorn beetles species collected from secondary forest was similar to the primary forest.Distribution pattern of longhorn beetles in Mt. Slamet indicated that some forest species were found at certain altitude such as Batocera spp and Gnoma thomsoni which only found at 700-1000m asl. The forest area which this altitute (700-1000m asl.) was located at the southern part of Mt Slamet (Kalipagu, Baturraden) where known as the main support of water reservoir for electric power and seven spout water (Pancuran Tujuh). Thus this area has to be conserved from illegal logging and human activities.

IDENTIFIKASI GALUR-GALUR PADI MUTANINSERSI TOLERAN DAN RENTAN CEKAMAN SALINITAS BERDASARKAN KARAKTER MULTIVARIAT PERTUMBUHAN DAN BIOKIMIA PADA FASE VEGETATIF Situmorang, Apriadi; Zannati, Anky; Widyajayantie, Dwi; Nugroho, Satya
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Growth and biochemical characters of thirty-three Nipponbare-based rice mutant lines independently harboring activation-tag in salt stress condition were investigated. Rice cv Nipponbare wild type, IR29 and Pokkali were used as isogenic, susceptible, and tolerant cultivars, respectively. Plants were grown in Yoshida nutrient solution containing NaCI (6 g/1) as the stress treatment and in Yoshida nutrient solution without NaCI as the control. Evaluation of salt tolerance was conducted at 21 days after salinization.The results showed that there was a significant difference in growth characters among genotypes in reduction of the plant height,root length, shoot fresh weight, root fresh weight, shoot dry weight, as well as in the root dry weight. Biochemical characters of the genotypes also showed significant difference in their reduction of chlorophyll-a and chlorophyll-b concentrations,total carotenoids and proline accumulation. Based on multivariate growth and biochemical characters,T3.PMO.VI.63.5a.30.9,T3.PMO.VI.30.1a.21.1, T3.PMO.V1.63.5a.33.7F3.PUR.IX.49.1d.l.6.5,T3.PMO.VI.81.3a.4.4, F3.PUR.IX.49.1d.l.6.5,and F3.PUR.VIII.5.1f.l.4.8 were assumed as potential salt-tolerant lines, respectively, while mutant lines T3.PMO.VI.30.1a.l07.7,T3.PMO.VI.63.5a.20.6,T3.PMO.III.4.4c.7.2,andT3.PMO.VI.30.1a.51.1 were assumed as salt-susceptible lines respectively.

STATUS INFEKSI VIRUS INFLUENZA PADA BEBERAPA SPESIES HEWAN SEBELUM WABAH AVIAN INFLUENZA H5N1 PADA UNGGAS DI INDONESIA Sendow, Indrawati; Adj id, RM Abdul; Selleck, Paul
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

After outbreak of Avian Influenza HPAI in chicken in mid 2003 in Indonesia, there was a question whether Avian Influenza HPAI was already presence in animals before the outbreak. A retrospective study was conducted to gain information on the presence of Influenza A virus infection in a range of animal species that could be infected by the virus. A total of 1529 animal sera, from 8 species from 12 different provinces which were stored at the Bbalitvet (Research Institute for Veterinary Science) Serum Bank were tested against matrix antigen of Influenza A using the Agar Gel Immunodiffusion (AGID) test. The results indicated that only 0.6% of animal tested which consisted of 4% of duck sera and 0.4% of pig sera were reacted in the AGID test with weak reaction. Those sera were then tested against Influenza A group viruses using an Enzyme Linked Immunosorbent Assay (ELISA), indicated that Influenza A viruses were not detected in either duck and pig positive sera. Those sera which were also tested by HI test against antigens of HI, H3 and H7, also indicated that none of those sera were reacted. In addition, 134 lung of pigs from an abattoir were collected for virus isolation. The viral isolation on chicken embryonated eggs resulted in 12 samples that contained viruses with agglutinated goose and chicken red blood cells. Identification of viruses isolated was done by agglutination test and ELISA. The results showed that none of those isolates were Influenza Type A virus. This study showed that influenza A virus group infection was not detected in animal species sampled before outbreak of AI H5N1 in 2003 in Indonesia.

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