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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 24 Documents

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Search results for , issue "Vol 15, No 3 (2016)" : 24 Documents clear

PERTUMBUHAN, PRODUKSI DAN POTENSI GIZI TERONG ASAL ENGGANO PADA BERBAGAI KOMBINASI PERLAKUAN PEMUPUKAN [The growth, production and nutrition potential of Enggano eggplant on various combinations of fertilizer treatments] Juhaeti, Titi; Lestari, Peni
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

This research was carried out to study growth, production and nutritional potential of two accessions of Enggano eggplant on various combinations of organic and anorganic fertilizer. The experiment was designed using to Factorial Randomized Complete Block with 5 replications. The first factor was two eggplant accessions (Meok accesion/round fruit and Malakoni accession/long finger fruit). The second factor was 9 combinations of organic and inorganic (NPK) fertilizer. The result showed that long finger eggplant more productive than rounded eggplant. The total fruit fresh weight of long finger eggplant (2383.3 g) higher than rounded eggplant (1016.4 g). The Â½ NPK + Â½ StarTmik fertilization treatment showed better vegetative growth than other treatments. Meanwhile, for total plant production, NPK treatment showed the highest fruit production followed by Â½ NPK +Â½ POKomersial and Â½ NPK +Â½ starTmik treatments. Â Nutritional analysis showed that long finger eggplant contained of vitamin C 15.887 g/100g, phosphor 25.983-27.440 mg/100g; calcium 12.340-13.274 mg/100g; fat 0.120-0.277%, carbohydrate 6.244-8.315% and crude fiber 0.872-1.110%. Meanwhile, round eggplant, Â contained of vitamin C 10.354 g/100g, phosphor 34.919-37.012 mg/100g; calcium 14.355-15.240 mg/100g; fat 0.275-0.338%, Â carbohydrate 6.733-7.896% and crude fiber 2.005-2.211%.

KUALITAS NUTRISI ANEKA TEPUNG DAN KUE TALAM BERBASIS BAHAN PANGAN PULAU ENGGANO DENGAN PENAMBAHAN Lactobacillus plantarum B110 [Nutrition Quality of Various Flour and Talam Cake Based on Enggano Island Food Material Added with Lactobacillus plantarum B110] Khusniati, Tatik; Sulistiani, Sulistiani; Choliq, Abdul; Nanta, Dhea Loka; Wardani, Dita Kusuma; Saraswati, Dahniar
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2222

Nine foodstuffs from Enggano island were processed to flour as wheat flour alternative. To increase flour quality and its derivative product, Lactobacillus-plantarum B110 was added those flour and talam cake made from such flour. They were forest cassava (Dioscorea sp.), ararut sago (Marantha arundinacea), tacca (Tacca Leontopetaloides), egg taro (Alocasia sp.), oil taro (Alocasia sp.), gadung (Dioscorea hispida), gogo rice (Oryza sativa), corn (Zea mays), and belinjo (Gnetum gnemon).Â The quality assessment consisted of HCN detection (qualitative), nutrition contents (proximate analysis), acidÂ (titration method), glucose (GOD-POD kit), and organoleptic tests (20 panelists). The results show that acid and glucose contents of the nine flours increased after L. plantarum B110 addition. Acid content ofÂ the flours was 0.0144-0.2475%, whileÂ glucose wasÂ 0.056-0.449%.Â Carbohydrate, energy and acid contents of L. plantarum B110 talam cake were higher than control, which those were 34.13%, 190.33 cal/100gr, 0.00082%, while protein, lipid, water, ash were 1.75%, 5.22%, 57.9 %, 1.01%, respectively. The talam cake was accepted by panelists with values: 5.50 (taste), 5.6 (colour), 4.55 (flavour), 4.00 (texture), 4.35 (homogeneous). It was concluded that the flour and talam cake quality increased with addition of L. plantarum B110.Â

AKTIVITAS ANTIBAKTERI AKTINOMISETES LAUT DARI PULAU ENGGANO [Antibacterial activity of marine actinomycetes from Enggano Island] Ratnakomala, Shanti; Apriliana, Pamella; Fahrurrozi, Fahrurrozi; Lisdiyanti, Puspita; Kusharyoto, Wien
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Marine actinomycetes were isolated from mangrove sediment from the coast in Enggano, Bengkulu Province, Indonesia using the medium NBRC No. 802 modified by the addition of 2% NaCl. A total of 29 isolates of actinomycetes were isolated from three mangrove sediment samples and evaluated their potential to produce bioactive metabolites. Screening of 29 isolates marine actinomycetes isolates were performed against three bacterial pathogens had been done. Bacteria test used was Escherichia coli NBRC 14 237, Staphylococcus aureus NBRC13 276, and Bacillus subtilis NBRC 3134. Screening result showed that seven isolates have inhibitory effects against bacteria test and 22 other isolates have no inhibition. Of the seven isolates, one isolate has inhibitory effect against the growth of Gram-negative bacteria Escherichia coli, while six other isolates inhibit Gram-positive bacteria Bacillus subtilis and Staphilococcus aureus. It was concluded that, of the 29 isolates conducted in the experiment, seven isolates produce antibacterial compounds on agar medium. Molecular identification of 23 isolates were identified based on Â the gene 16S RNA sequences showed that 22 isolates belong to the genus Streptomyces and one strain belongs to the genus Dermacoccus.

KERAGAMAN AKTINOMISETES ASAL SERASAH, SEDIMEN, DAN TANAH PULAU ENGGANO, BENGKULU [Deversity of Actinomycetes From Soil, Sediment, and Leaf Litter Samples of Enggano Island, Bengkulu] Putri, Ade Lia; Nurkanto, Arif
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A total of 344 isolates of actinomycetes were isolated from leaf litter and soil samples collected from four locations of Enggano Island, Bengkulu. eighty eight isolates were selected using morphological characteristics and identified using 16S rRNA gene sequence analysis. Three families (Micromonosporacea, Streptomycetaceae, and Streptosporangiaceae) and eight genera were found from these samples. Approximately 80% of these strains belonged to Streptomyces group and 20% of isolates were similar to rare actinomycetes. Based on homology search (BLAST) and phylogenetic tree analysis, isolates of rare actinomycetes (EgA15, EgA30, EgA85, EgA386, EgA236, EgA243, EgA335, EgA112, EgA41,EgA312, EgA314) were identified as Kitasatosporia paracochleata (100%), Kitasatospora azatica (99%), Kitasatospora griseola (99%), Sphaerisporangium album (99%), Actinoplanes nipponensis (99%), Pseudosporangium ferrugineum (99%), Nonomuraea rosela (99%), Nonomuraea guangzhouensis (98%), Micromonospora chaiyaphumensis (99%),Micromonospora chaiyaphumensis (99%), and Couchioplanes caeruleus (99%) respectively.

SKRINING BEBERAPA JAMUR ENDOFIT TUMBUHAN DARI PULAU ENGGANO, BENGKULU SEBAGAI ANTIBAKTERI DAN ANTIOKSIDAN [Screening of Plant Endophytic Fungi from Enggano Island, Bengkulu for Antibacterial and Antioxidant Activites] wulansari, dewi; Putra, Aldho Pramana; Ilyas, Muhammad; Praptiwi, Praptiwi; Fathoni, Ahmad; Palupi, Kartika Dyah; Agusta, Andria
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Fungal endophytes have been known as sources for bioactives with high chemical structure variability. This study aimed to screen extracts of some endophytic fungi associated with plants from Enggano Island as antioxidant and antibacterial using thin layer chromatography (TLC) bioautography method. Antibacterial ativity was performed against Escherichia coli and Staphylococcus aureus. Seventeen out of 22 extracts showed growth inhibition activity against both bacteria, while 16 extracts were active as DPPH free radical scavenging agents. Further determination on IC50 value of four prominent extracts revealed that fungal endophytes AK3018-1, FC-1, KC-4, and SO-3 have IC50 value of 85, 84, 704, and 347 ?g/ml, respectively in DPPH radical scavenging method. Extracts of fungal endophytes CR-3, CS-2, and SM-2 showed prominent antibacterial activity among other extracts, indicated by wide clear white zone around the spot. Further evaluation on minimum inhibitory concentration (MIC) of those three extracts by microdilution method showed that CR-3, CS-2, and SM-2 have MICs value of 512, >512, and 64Âµg/ml, respectively against S. aureus.Fungal endophytes AK3018-1, FC-1, KC-4, SO-3, CR-3, CS-2, and SM-2 were respectively isolated from Dioscorea bulbifera tuber, Fibraurea chloroleuca twig, Knema cinerea twig, Smilax odoratissima stem, Cryptocarya sp. twig, Calophyllum soulattri twig, and Smilaxmacrophylla stem.

EVALUASI ANTIBAKTERI DAN ANTIOKSIDAN EKSTRAK SMILAX spp. DARI PULAU ENGGANO [Evaluation of Antibacterial and Antioxidant of Smilax spp. Extracts Collected from Enggano] Tiwi, Praptiwi; Palupi, Kartika Dyah; Fathoni, Ahmad; Keim, Ary P; Royani, M Fathi; Effendi, Oscar; Agusta, Andria
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Three species of Smilax spp. (Smilax macrophylla, S. odoratissima and S. zeylanica) collected from Enggano island were evaluated for their potential as an antibacterial and antioxidant. Stems and leaves of three species of Smilax spp. were extracted successively with nhexane,chloroform, ethyl acetate and methanol. Antibacterial activity was evaluated by TLC-bioautography against Eschericha. coli InaCC B5 and Staphylococcus. aureus Ina-CC B4. The antioxidant activity was analyzed by DPPH free radical activity by bioautography method. The value of minimum inhibitory concentration (MIC) and IC50 of active extracts were done by serial dilution in 96- well microplate. The results showed that 20 extracts have antioxidant activity, 13 extracts inhibited the growth of S. aureus Ina-CC B4, and 14 extracts inhibited the growth of E. coli Ina-CC B5. MIC values of active extracts against S. aureus Ina-CC B4 were in the range of 128 - > 512 Âµg / ml, while the values of MIC against E. coli B5 Ina-CC were > 512 Âµg / ml. IC50 values of extracts that has antioxidant activity were in the range of 184.11-4549.34 mg/L.

ANALISIS FRONT SALINITAS BERDASARKAN MUSIM DI PERAIRAN PANTAI BARAT SUMATERA [Analysis of Salinity Front by Season in the Coastal West of Sumatra] Supiyati, Supiyati; Suwarsono, Suwarsono; Astuti, Nissa
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Technology of the marine sciences, especially the potential Â resources of fish can be evalute from front area identification. The aim of this research is to identify front salinity area based on seasonal variation in the west coastal of Sumatra. The method involved modeling by ODV Â (Ocean Data View), Fortran, Transform, and Matlab software. We used salinity data what obtained from NOAA (National Oceanic and Atmospheric Administration). The result showed that vertically, front areas in coastal West of Sumatera for all off season was in depth of Â± 41.6 meters, 83.2 meters and 124.8 meters. In the North West Monsoon, salinity front area was around of the Nias, Simeulue, Northwest and southeast Â of Enggano islands. On the first of transition period was in West of Simeulue, southeast of Nias, and East Enggano Islands. In the South East Monsoon was in the Northeast of Simeulue, the East of Mentawai, Southeast and Northwest of Enggano Islands. On the second transition period was in the Eastern of Nias and Mentawai Islands. Salinity changes of fourth monsoons on this front area is Â± 0,5 psu.

VARIASI DAN DEGRADASI SUARA PANGGILAN KODOK JANGKRIK [HYLARANA NICOBARIENSIS (STOLICZKA, 1870)] ASAL PULAU ENGGANO (ANURA: RANIDAE) [Variation and degradation on advertisement calls of Cricket Frog, Hylarana nicobariensis (Stoliczka, 1870) from Enggano Island] Kurniati, Hellen; Hamidy, Amir
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Frogâs group of Hylarana nicobariensis (Stoliczka, 1870) is a complex species that has diverse genetic populations and variations on advertisement calls. Variations on advertisement calls among populations have been reported in Sumatra, Java and Bali. The population of frog in Enggano has different phenomenon that was not found in Sumatra and Java. Â Enggano population was genetically identical, but the advertisement calls were very diverse. There were four types of advertisement calls on the population in Enggano Island, namely: (1) advertisement call type 1 which has pulse wave with the dominant frequency of 2855.13 Â± 135.22 Hertz; (2) advertisement call type 2 which has pure tone wave with the dominant frequency of 2720.29 Â± 97.69 Hertz; (3) advertisement call type 3 which has pulse wave with dominant frequency 2634.61 Â± 292.81 Hertz; (4) advertisement call type 4 which has pure tone wave with dominant frequency 1618.79 Â± 83.05 Hertz. The degradation of the dominant frequency and the lower frequency was likely due to the increase of snout to vent length (SVL) of adult male that were followed by increasing the size of the head.

DIVERSITY OF XYLOSE ASSIMILATING YEAST FROM THE ISLAND OF ENGGANO, SUMATERA, INDONESIA [Keragaman Khamir Pengguna Xilose yang Diisolasi dari Pulau Enggano, Sumatera, Indonesia] Kanti, Atit; Sumerta, I Nyoman
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Naturally occurring yeasts (wild type) are important genetic resources for many industrial interests. Though biodiversity exploration of Enggano has been done since 1944, but there is no report for yeast diversity from this island. Due to its industrial interest, xylose fermenting yeast is target for many scientists. This present study aims to isolate xylose assimilating yeast from island of Enggano. The samples were collected from varying sources included soil, leaf litter, decay wood, fruit and sediment. Xylose enrichment culture technique was performed to enrich xylose fermenting yeast. While for the isolation of yeast several methods which include dilution, ballistopore falling, direct isolation, membrane filtration were done. Of 200 isolates, 76 strains (38 %) were xylose assimilating yeast which mostly belong to Candida which include C. insectorum, C. tropicalis, C. boidinii, C. pseudolambica, C. yuanshanica, C. silvae, Cyberlindnera saturnus, Williopsis saturnus and Sporobolomyces poonsookiae. One strain Candida sp. that was isolated from soil could be assigned as a candidate of novel species on base on its morphological and biochemical characteristics along with analyses of gene sequence from D1/D2 domain. This study confirms that yeast survey is very important to obtain new genetic resources for industrial interest as well as for taxonomic study.

KEANEKARAGAMAN JAMUR ARBUSKULA DI PULAU ENGGANO [Diversity of Arbuscular Fungi in Enggano Island] Kramadibrata, Kartini
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A study on diversity of arbuscular fungi (AF) in forest area and small plantation in eastern part of Enggano island was done. Twelve species of AF were identified from this island. Eleven species had been reported from Java and associated with different kinds of plant, but three species i.e. Â Gigaspora gigantea, Glomus multicaule and Scutellospora heterogama were not common, while Sclerocystis taiwanensis is a new record for Indonesia. All species of AF recorded from this island were described by their morphological characters and their Â distribution in Enggano island were presented.

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