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Pengaruh Olahraga Aerobik Tipe Closed Skill Terhadap Konsentrasi Mahasiswa Kedokteran Universitas Airlangga Andira, Dzulqarnain; Argarini, Raden; Maramis, Margarita Maria
Journal Sport Area Vol 4 No 1 (2019): Juni
Publisher : UIR Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25299/sportarea.2019.vol4(1).2195

This research was conducted to determine the effect of using aerobic of closed-skill type toward students’ concentration of Pediatric Department of Universitas Airlangga. This research was experimental design which randomized pre-test post-test control group with 36 students who seldom doing sport as samples. These students were divided into two groups; experimental group and control group. Experimental group did aerobic with closed technique type training which medium to high intensity around 75 minutes a week for 3 weeks. On the otherwise, control group did not do it. The research instruments were xbox with kinetic sensor and attention network test. The students’ concentration was measured three aspects such as alerting, orienting and conflict effect. This analysis technique was done by using statistical technique through SPSS. The result shown that there was no significant different between experimental group and control group by comparing average score of pre-test and post-test for alerting aspect (0.895), orienting (0.572), and conflict effect (0.019). Therefore, there was significant different from pre-test and post-test of control group for conflict effect (0.019) but there were not significant different for alerting (0.072) and orienting (0.735) and for experimental group, there was significant different in alerting (0.02) and conflict (0.001) but there was not significant different for orienting effect (0.653). It can be concluded that there was not significant effect on students’ concentration between control group (0.778) and experimental group (0.571). It means that aerobic cannot influence students’ concentration.

PERBANDINGAN LATIHAN KONTINU INTENSITAS SEDANG DAN LATIHAN INTERVAL INTENSITAS TINGGI TERHADAP KONTROL GLUKOSA DARAH Argarini, Raden; Atsari, Nadhila; Susanto, Hermawan
Majalah Kedokteran Bandung Vol 48, No 4 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Pencegahan diabetes melitus tipe 2 (DMT2) dapat dilakukan dengan memperbanyak latihan fisik. Latihan kontinu intensitas sedang (LKIS) dan latihan interval intensitas tinggi (LIIT) diketahui dapat meningkatkan kontrol glukosa pada orang dengan resistensi insulin/DMT2. Tujuan penelitian ini adalah membandingkan efek LIIT dan LKIS pada orang sehat terhadap kontrol glukosa darah, yaitu glukosa darah puasa (GDP) dan tes toleransi glukosa oral (TTGO). Laki-laki (n=27), usia (20,07±0,62), IMT (23,94±3,59) kg/m2 berpartisipasi dalam kelompok LKIS, LIIT, atau kontrol. Latihan Ergocycle dilakukan 3 sesi/minggu selama 4 minggu; LKIS dengan total durasi 46 menit/sesi (intensitas sedang 50-60% denyut jantung cadangan [HRR] selama 40 menit); LIIT dengan total durasi 24 menit/sesi (6 siklus; 2 menit intensitas tinggi 80?90% HRR + 1 menit intensitas sedang 50?60% HRR). Kontrol tidak mendapat intervensi latihan. GDP dan TTGO (120? setelah beban glukosa) diperiksa 3 hari sebelum dan setelah program latihan. Hasil penelitian menunjukkan GDP menurun pada semua kelompok, tetapi hanya LKIS yang menunjukkan penurunan signifikan (p=0,048). TTGO ditemukan tidak berubah di semua kelompok (p>0,05). LKIS memiliki potensi untuk meningkatkan kontrol glukosa darah pada subjek dewasa muda. Penelitian lanjutan dengan modifikasi dosis latihan diperlukan untuk mengetahui efek LIIT lebih lanjut. [MKB. 2016;48(4):194?9]Kata kunci: kontrol glukosa darah, latihan interval intensitas tinggi, latihan kontinu intensitas sedangComparison of Moderate Intensity Continuous Training and High Intensity Interval Training on Blood Glucose ControlAbstractType 2 diabetes mellitus (T2DM) can be prevented by intensive physical exercise/training. Moderate intensity continuous training (MICT) and high intensity interval training (HIIT) are known to improve glucose control in people with insulin resistance and T2DM. The purpose of this study was to compare the effects of MICT and HIIT in healthy people on blood glucose levels, which was measured through fasting blood glucose (FBG) and oral glucose tolerance test (OGTT). Healthy men (n=27), aged (20.07 ± 0.62), BMI (23.94 ± 3.59) kg/m2 participated in either MICT, HIIT, or Control group (n=9 each group). Ergocycle exercise were performed 3 times/week for 4 weeks; MICT with a total duration of 46 minutes/session (moderate intensity 50-60% heart rate reserve [HRR]) for 40 minutes); HIIT with a total duration of 24 minutes/session (6 cycles; 2 mins high intensity 80-90% HRR + 1 min moderate intensity 50-60% HRR). Controls did not receive any programmed training. FBG and OGTT (120? after glucose load) were checked 3 days before and after the exercise program. This study found that FBG decreased in all groups but only the MICT group showed a significant reduction (p=0.048). OGTT was found unaltered in all groups (p>0.05). MICT has the potential to improve blood glucose control in healthy young adult subjects. However, further research with exercise dose modification is required to elucidate the effects of HIIT. [MKB. 2016;48(4):194?9]Key words: Blood glucose control, high intensity interval training, moderate intensity continuous training

PENGARUH EKSTRAK BAYAM MERAH (AMARANTHUS GANGETICUS) TERHADAP MORFOLOGI STRATUM HIPOKAMPUS MODEL ANAK MENCIT PASCASAPIH INDUK YANG TERPAPAR TIMBAL SELAMA MASA KEHAMILAN Kalanjati, Viskasari; Pratiwi, Made Pury; Syakdiyah, Noer Halimatus; Widiasi, Etha Dini; Anggraeni, Mayang Rizki; Pratiwi, Intan Anggun; Argarini, Raden
Majalah Kedokteran Bandung Vol 46, No 3 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1418.333 KB)

Kandungan antioksidan ekstrak bayam merah (Amaranthus gangeticus) diduga dapat melawan efek toksik timbal asetat pada sistem saraf pusat. Penelitian ini bertujuan untuk mengamati pengaruh pemberian ekstrak bayam merah terhadap morfologi hipokampus model anak mencit (Mus musculus) pascasapih yang induknya terpapar timbal asetat per oral selama masa kehamilan. Penelitian dilakukan di Laboratorium Hewan Coba Fakultas Kedokteran, Universitas Airlangga (FKUA) pada bulan April?Juni 2013. Dua puluh tujuh ekor anak mencit pascasapih dibagi dalam 3 kelompok: M0 (sodium carboxymethyl cellulose atau CMC Na 0,5%), M1 (CMC Na 0,5%+timbal asetat 1,16 mg/10 g BB/hari) dan M2 (CMC Na 0,5%+timbal asetat 1,16 mg/10 g BB/hari+ekstrak bayam merah 382,2 mg/10 g BB/hari). Sediaan otak diambil untuk dibuat preparat histologi dengan pewarnaan hematoxylin-eosin (HE). Lebar stratum granulare girus dentatus (GD), stratum oriens-piramidale (SOP) CA1, CA2, dan CA3 dianalisis dengan analysis of variance (ANOVA) dan least significant different (p<0,05). SOP CA1 dan CA2 pada kelompok M2 lebih lebar dibandingkan dengan kelompok M1 (p<0,05). SOP CA1 kelompok M2 lebih lebar pula bila dibandingkan dengan M0 (p=0,001). Lebar SG dari GD dan SOP CA3 kelompok M2 menunjukkan nilai yang lebih tinggi (p>0,05). Simpulan, pemberian ekstrak bayam merah dapat mengurangi efek negatif timbal asetat yang merusak struktur hipokampus model anak mencit pascasapih yang induknya terpapar timbal selama kehamilan. Kata kunci: Bayam merah, hipokampus, timbal asetatEffect of Red Spinach (Amaranthus gangeticus) Extract on Hippocampus Morphology of Post-Weaning Mice Infant Model from Lead-Acetate Exposed Pregnant Mice Antioxidants in red spinach (Amaranthus gangeticus) extract are proposed to combat the toxicity of lead acetate in the central nervous system. The effect of red spinach extract to the morphology of post weaning mouse hippocampus model (Mus musculus) in pregnant mice that received oral lead acetate during pregnancy was analyzed. Twenty seven post-weaning mice offsprings were grouped into 3 groups: M0 (sodium carboxymethyl cellulose or CMC Na 0.5%), M1 (CMC Na 0.5%+lead acetate 1.16 mg/10 g of body weight/day) and M2 (CMC Na 0.5%+lead acetate 1.16 mg/10 g of body weight/day+red spinach extract 382.2 mg/10 g of body weight/day). Brains were histologically processed and stained with hematoxylin-eosin (HE). Width of stratum granulare (SG) of the dentate gyrus (GD) and each of stratum oriens-pyramidale (SOP) of CA1, CA2, CA3 from each mouse hippocampus were obtained and analyzed statistically using analysis of variance (ANOVA) and least significant difference (LSD) (p<0.05). The CA1 and CA2 SOPs in M2 were significantly wider compared to those of M1 (p<0.05). Meanwhile, the SOP of CA1 in M2 was significantly wider compared to that of M0 (p=0.001). The SG width of GD and the CA3 SOP in M2 were wider than those of M0 and M1 (p>0.05). In conclusion, red spinach extract might dampen the adverse effects of oral lead acetate in post-weaning mouse hippocampus model from pregnant mice orally exposed to lead acetate during pregnancy. Key words: Hippocampus, lead acetate, red spinach DOI: 10.15395/mkb.v46n3.116

Pemberian Ekstrak Bayam Merah (Amarantus Tricolor linn) Memperbaiki Ekspresi BDNF dan GLUR1 pada Area Hipokampus Tikus Muda yang Terpapar Anestesi Inhalasi Halothane dan Sevoflurane Argarini, Raden; Wigati, Kristanti Wanito; Herawati, Lilik; Riawan, Wibi; Rehatta, Nancy M.
Majalah Kedokteran Bandung Vol 48, No 3 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Otak dalam tahap perkembangan memiliki tingkat kerentanan lebih tinggi terhadap zat anestesi dibanding dengan otak orang dewasa. Penelitian ini bertujuan mengamati pengaruh pemberian ekstrak bayam merah terhadap penanda biologis fungsi kognitif dan neuroplastisitas, yaitu Brain Derived Neurotrophic Factor (BDNF) dan AMPA Glutamate Receptor subunit 1 (Glur1) pada area hipokampus tikus yang terpapar anestesi inhalasi halothane dan sevoflurane. Rancangan penelitian adalah randomized posttest only control group. Penelitian ini dilakukan di Departemen Biokimia Fakultas Kedokteran Universitas Airlangga dan prosedur anestesi dilakukan di Rumah Sakit Hewan Fakultas Kedokteran Hewan Universitas Airlangga pada bulan Mei 2014â€“Oktober 2014. Subjek penelitian adalah 23 ekor tikus putih jantan (Rattus norvegicus) galur Wistar, umur 1â€“1,5 bulan, bobot kurang lebih 60â€“100 gram dengan kondisi sehat fisik. Subjek dibagi menjadi 5 kelompok, yaitu K (kontrol); K1 (halothane 1 MAC); K2 (bayam merah 800 mg/kgBB+Halothane 1 MAC); K3 (sevoflurane 1MAC) dan K4 (bayam merah 800 mg/kgBB+ Sevoflurane 1 MAC). Hasil penelitian menunjukkan adanya penurunan ekspresi BDNF pada pemberian halothane dan sevoflurane serta peningkatan kembali ke normal pada kelompok yang sebelumnya telah diberikan bayam merah sebelum terpapar gas anastesi (p=0,000). Ekspresi GLUR1 meningkat pada pemberian halothane dan sevoflurane dan penurunan kembali ke normal pada kelompok yang sebelumnya telah diberikan bayam merah sebelum terpapar gas anestesi (p=0,000). Simpulan penelitian ini adalah pemberian ekstrak bayam merah memiliki potensi untuk mencegah neurotoksisitas pada fungsi kognitif tikus yang terpapar gas anastesi. [MKB. 2016;48(3):148â€“54]Kata kunci: AMPA Glutamate Receptor subunit 1 (GLUR1), Brain Derived Neurotrophic Factor (BDNF), ekstrak bayam merah, halothane, sevofluraneRed Spinach Extract Administration (Amarantus Tricolor linn) in Young Rats Exposed to Inhalation Anesthetic Halothane and Sevoflurane Improves BDNF and GLUR1 Expressions in Hippocampus AreaIn the development stage, brain has a higher level of vulnerability than the adult brain to anesthetic agents. The aim of this study was to investigate the effects of red amaranth extract to brain derived neurotrophic factor (BDNF) and AMPA glutamate receptor subunit 1 (Glur1) expressions as biomarkers of cognitive and neuroplasticity in rat hippocampus areas exposed to inhaled anesthetics halothane and sevoflurane. The design of this study was a randomized posttest only control group. This study was conducted at the Department of Biochemistry Faculty of Medicine, Universitas Airlangga, and the anesthetic procedures were performed at the Veterinary Hospital, Faculty of Veterinary Medicine Universitas Airlangga during the period of Mayâ€“October 2014. The subjects of this study were 23 white male rats (Rattus norvegicus) wistar strain, aged 1â€“1.5 months, weighted approximately 60â€“100 grams in a healthy physical condition. The subjects were divided into 5 groups, K (control); K1 (1 MAC halothane); K2 (red amaranth 800 mg/kgBW + 1 MAC halothane); K3 (1 MAC sevoflurane); and K4 (red amaranth 800mg/kgBW+1 MAC sevoflurane). The results of this study showed a decrease in the expression of BDNF when halothane and sevoflurane were administered. Red amaranth treatment prior to anesthetic gases exposure preserved and increased BDNF expression (p=0.000) while GLUR1 expression increased in the group that received halothane and sevoflurane administration and decreased back to normal in the group that received red amaranth treatment prior to exposure to anesthetic gases (p=0.000). In conclusion, red amaranth extract has the potential effect to prevent cognitive neurotoxicity on the cognitive function in rats exposed to anesthetic gases. [MKB. 2016;48(3):148â€“54]Key words: AMPA Glutamate Receptor subunit 1 (GLUR1), Brain Derived Neurotrophic Factor(BDNF), halothane, sevoflurane, red amaranth extractÂ

Red Dragon Fruit Extract (Hylocereus polyrhizus) Restores Learning Ability and Memory on Test Animals Post Lead Exposure: Experimental Study on Test Animals Made Pury Pratiwi; Raden Argarini; Sudarno Sudarno
ANIMA Indonesian Psychological Journal Vol. 30 No. 1 (2014): ANIMA Indonesian Psychological Journal (Vol. 30, No. 1, 2014)
Publisher : Laboratory of General Psychology, Faculty of Psychology, Universitas Surabaya

The aim of this study was to analyze whether red dragon fruit (Hylocereus polyrhizus) administration affect learning ability and memory after lead exposure. A randomized control-group pretest-posttest was conducted with 24 mice (Mus musculus), which was divided through a random assignment into four groups: G1, G2, G3, and G4. Group G1 received 1.16 mg/10 g BW lead acetate. Group G2, G3, and G4 received 1.16 mg/10 g BW lead acetate and red dragon fruit extract 250 mg/kg BW (G2), 500 mg/kg BW (G3), and 1000 mg/kg BW (G4). Results revealed that some groups that has been given red dragon fruit extract (group G2, G3, and G4) showed significant improvement of latency time, frequency, and combined scoring value of latency time and frequency in posttest by using Morris Water Maze compared to the control group (p = .001; α = .05). The improvement of group G3 and G4 was better than group G2. These finding indicates that administration of red dragon fruit extract have a protective effect and improves learning ability and memory after lead exposure with 500-1000 mg/kg BW as the best dose.

LATIHAN INTERVAL INTENSITAS TINGGI MENURUNKAN TEKANAN DARAH SISTOL ISTIRAHAT TETAPI TIDAK MENURUNKAN TEKANAN DARAH DIASTOL DAN DENYUT NADI ISTIRAHAT PADA DEWASA MUDA SEHAT NORMOTENSIF Naesilla -; Raden Argarini; Indri Safitri Mukono
Sport and Fitness Journal Volume 4, No. 1, 2016
Publisher : Program Studi Magister Fisiologi Keolahragaan, Fakultas Kedokteran, Universitas Udayana

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Kebugaran kardiorespiratori adalah sebuah penentu kuat morbiditas dan mortalitas. Tujuan dari studi ini adalah untuk mengamati pengaruh dari latihan interval intensitas tinggi (HIIT) pada tekanan darah dan denyut jantung pada kondisi istirahat pada dewasa muda normotensif. Desain studi adalah pretestposttest experimental design. Subyek penelitian adalah 10 orang dewasa muda. Subyek melakukan HIIT sebanyak 6 siklus (tiap siklus terdiri dari 2 menit latihan intensitas tinggi 80-90% reserve heart ratedan 1 menit latihan intensitas sedang 50-60% reserve heart rate). Tiap latihan, subyek melakukan pemanasan selama 3 menit, diikuti sesi HIIT, dan diakhiri dengan latihan pendinginan selama 3 menit. Subyek melakukan sesi latihan selama 3x seminggu selama 4 minggu dengan ergocycle. Data pretest diambil 3 hari sebelum sesi latihan dimulai dan data posttest 3 hari sesudah sesi latihan berakhir. Data dianalisis dengan SPSS menggunakan paired t-test. Hasil signifikan didapatkan pada tekanan darah sistol istirahat (p= 0,020). Tekanan darah diastol dan denyut nadi istirahat didapatkan tidak signifikan (p=0,470 and p=0,185). Kesimpulan, HIIT selama 4 minggu mampu menurunkan tekanan darah sistol istirahat tetapi tidak memiliki efek pada tekanan darah diastol dan denyut nadi istirahat pada dewasa muda sehat normotensif.

PENGARUH PEMBERIAN GLUTATHIONE PRA LATIHAN SUBMAKSIMAL TERHADAP JUMLAH TROMBOSIT DAN MASA PERDARAHAN : STUDI EKSPERIMENTAL PADA HEWAN COBA Hesty Rohmatul Fajri; Raden Argarini; Choesnan Effendi
Sport and Fitness Journal Volume 3, No.1, 2015
Publisher : Program Studi Magister Fisiologi Keolahragaan, Fakultas Kedokteran, Universitas Udayana

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Submaximal activity leads to oxidative stress that causes cellular damage, including platelet. Glutathione as a potential antioxidant has cellular protective effect. The purpose of this research was to analize the effect of glutathione pre submaximal exercise on platelet count and bleeding time. The study used separate post test only control group design with 28 male rats (Rattus norvegicus, Wistar strain) which were divided into four groups : P1(6) without intervention; P2(7), P3(8), and P4(7) with administration of placebo (aquabidest), glutathione 54 mg/kgBB, and glutathione 108 mg/kgBB intraperitoneally for each group that were given 5 times, continued with submaximal exercise (swimming with 4% of weight load). The result of ANOVA and LSD test showed that submaximal exercise had tendency to decrease platelet count (p=0,407; not significant) and prolonged bleeding time (p=0,017). Glutathione pre submaximal exercise had tendency to increase platelet count (p=0,407; not significant) and shortened bleeding time (p=0,017). Glutathione with a dose of 54 mg/kg had protective effect on platelet better than a dose of 108 mg/kg. In conclusion, the administration of glutathione pre submaximal exercise has protective effect on platelet in male rats (Rattus norvegicus).

COMPARISON EFFECT OF CV 12, ST 36 AND ST 40 EA ON SHORT TERM ENERGY BALANCE REGULATION IN HIGH FAT DIET RAT Purwo Sri Rejeki; Harjanto Harjanto; Raden Argarini; Imam Subadi
Folia Medica Indonesiana Vol. 52 No. 3 (2016): JULY - SEPTEMBER 2016
Publisher : Faculty of Medicine, Universitas Airlangga

The aim of this study was to determine the comparative effects of EA (EA) on the CV12, ST36 and ST40 to weight gain prevention over the short-term regulation of energy balance. The study was conducted with a completely randomized design. Rats were divided into five groups: negative control group (no treatment, n=5), positive control (sham EA/back, n=5), EA CV 12 (n=6), EA ST 36 (n=6) and EA ST 40 (n=7). Rats were exposed to high-fat diet for two weeks and EA was simultaneously performed once daily, five days a week for two weeks with 2 Hz, for 10 minutes with continuous wave. Body weight, BMI, front limb circumference and rear were measured during study. Levels of blood glucose, cholesterol, triglycerides, LDL and HDL were measured at the end of the study; which reflects the short-term regulation of energy homeostasis. For weight loss, EA CV12, ST36 and ST40 group have lost weight significantly compared to the negative and positive control group. The ST40 group has a significant decrease than ST36 and CV12. The most significant decrease in BMI found in the ST40 group. EA did not affect blood glucose levels, but modulated blood lipid profile. In ST 40 group there was a significant decrease in cholesterol, LDL and triglycerides. EA at point ST 40 is potential in preventing increased body weight and BMI in rats exposed to high-fat diet compared to the CV 12 and ST 36. ST 40 is a point with a potential of lowering LDL and triglycerides serum so that it can play a role in the short term regulation of energy homeostasis but also in the prevention of dyslipidemia.

High Calorie Diet with a Combination of Intermittent Restriction Affects the Reproductive Cycle and The Uterine Weight of Mice (Mus Musculus) Anggraeni Dyah Kumalasari; Lilik Herawati; Raden Argarini; Lina Lukitasari; Zulhabri Othman; Astika Gita Ningrum
Folia Medica Indonesiana Vol. 57 No. 4 (2021): December
Publisher : Universitas Airlangga

Highlight:High-calorie diet influence with interval restrictions combination on the reproductive cycle and weight of the uterus in mice were analyzed.Calories balance impact for female reproductive health. Abstract :Balanced calories affected for body health and daily activities. Lose of energy can disturb the health and daily activities, while over calorie for diets also causes disorders such as metabolic. When there are excess calories in the body will be involved. It can be an obesity risk, diabetes mellitus, fertility disorder, cancer, and cardiovascular disease. Furthermore, in women there can be an increase in menstrual disorders and fertility disorder risk (Silvestris et al., 2018). This study aims to analyze highcalorie diet influence with interval restrictions combination on the reproductive cycle and weight of the uterus in mice. Experimental laboratory is a mouse (mus musculus) female’s balb/c as object population. Sampling techniques using probability sampling with simple random sampling type. The study results on fisher's exact test gave a result of p > 0.05 so there was no significant difference between control group, high calorie diet group, and high calorie diet with interval restrictions group on the results of vaginal swabs /post-treatment reproductive cycle. The study outcome on robust tests between control, high calorie diet, and high calorie diet with interval restrictions groups. Obtained results (p < 0.05) that mean there are some significant discrepancies in the weight of uterine organ between groups. Calories balance in the body can impact female reproductive health.

Pengaruh Olahraga Aerobik Tipe Closed Skill Terhadap Konsentrasi Mahasiswa Kedokteran Universitas Airlangga Dzulqarnain Andira; Raden Argarini; Margarita Maria Maramis
Journal Sport Area Vol 4 No 1 (2019): June
Publisher : UIR Press

This research was conducted to determine the effect of using aerobic of closed-skill type toward students’ concentration of Pediatric Department of Universitas Airlangga. This research was experimental design which randomized pre-test post-test control group with 36 students who seldom doing sport as samples. These students were divided into two groups; experimental group and control group. Experimental group did aerobic with closed technique type training which medium to high intensity around 75 minutes a week for 3 weeks. On the otherwise, control group did not do it. The research instruments were xbox with kinetic sensor and attention network test. The students’ concentration was measured three aspects such as alerting, orienting and conflict effect. This analysis technique was done by using statistical technique through SPSS. The result shown that there was no significant different between experimental group and control group by comparing average score of pre-test and post-test for alerting aspect (0.895), orienting (0.572), and conflict effect (0.019). Therefore, there was significant different from pre-test and post-test of control group for conflict effect (0.019) but there were not significant different for alerting (0.072) and orienting (0.735) and for experimental group, there was significant different in alerting (0.02) and conflict (0.001) but there was not significant different for orienting effect (0.653). It can be concluded that there was not significant effect on students’ concentration between control group (0.778) and experimental group (0.571). It means that aerobic cannot influence students’ concentration.

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