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RISK FACTORS OF ANGULAR CHEILITIS AT GUSTI HASAN AMAN DENTAL HOSPITAL SOUTH KALIMANTAN Aulia Azizah; Selviana Rizky Pramitha; Maharani Laillyza Apriasari
Dentino : Jurnal Kedokteran Gigi Vol 6, No 2 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i2.12002

Background: The second highest burden of all diseases in world population includes infectious disease with a total of 28% cases. Angular cheilitis is an infectious condition on the corner of the mouth that may initiate masticatory, facial expression and speech dysfunction due to the involvement of orbicularis oris muscle surrounding the lips. The management of angular cheilitis comprises the elimination of causative microbes using antifungal agent. Therapeutic failure reveals the disregard of any related risk factor. Purpose: To analyze the risk factors of angular cheilitis in Gusti Hasan Aman Dental Hospital year 2019. Method: This study resorted analytical descriptive design of quantitative approach using cross-sectional method. Samples were 65 dental statuses of patients in Oral Medicine Clinic of Gusti Hasan Aman Dental Hospital year 2019. Result: Univariate data analysis presented the distribution of children category aged ≤ 18 years old (75.8%), men (54.8%), not consuming vegetable and fruit behaviour (46.8%), duration of treatment 1-7 days (56.4%), and incidence of angular cheilitis (75.8%). Fisher exact test unveiled the relationship between age and angular cheilitis (p<0.05) in which age ≤ 18 years old was 4.266 fold higher to experience angular cheilitis, while the relationship between gender and angular cheilitis (p<0.05) demonstrates the predilection of male with 4.853 fold at risk than female. Conclusion: Age and gender are risk factors in the incidence of angular cheilitis at Gusti Hasan Aman Dental Hospital year 2019. Keywords : Age, Angular Cheilitis, Behavior, Gender

THE RELATIONSHIP BETWEEN THE SEVERITY OF MALOCCLUSION AND GINGIVAL STATUS IN STUDENTS OF SMPN 5 MARABAHAN Akhmad Aufayed Ma’rifatullah; Diana Wibowo; Aulia Azizah
Dentino : Jurnal Kedokteran Gigi Vol 6, No 1 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i1.10633

ABSTRACTBackground: South Kalimantan experienced dental and mouth problems with a prevalence of 59,6%, so did Barito Kuala Regency with 68,66%. Malocclusion is a dental and oral health problem related to the disharmony between jaws and teeth. Many cases of malocclusion occur in children aged 12-14 years by 15,6% in the form of crowded teeth in South Kalimantan. Malocclusion is a factor that can cause food debris to stick to the interdental area between the crowded teeth, thus forming plaque accumulation. Plaque is the primary etiological factor that influences gingival status. Objective: This study aimed to determine the relationship between the severity of malocclusion and gingival status in students aged 12-14 years at SMPN 5 Marabahan. Method: This study used analytic survey research with cross sectional research design. The research population was all students of SMPN 5 Marabahan aged 12-14 years who born in March 2005 - January 2008, amounting to 88 people. The minimum sample size which determined by the proportion estimation formula was 47 people. The used sampling technique was simple random sampling technique with inclusion and exclusion criteria. Results: The distribution frequency of the study showed mild malocclusion of 55,3% and mild inflammation of 57,4%. Spearman analysis test results on the severity of malocclusion based on Mal I and gingival status based on GI showed a significance value of p = 0,045 (p < 0,05). Conlclusion: It could be concluded that there was a relationship between the severity of malocclusion and gingival status in students aged 12-14 years at SMPN 5 Marabahan.Keywords:Gingival Index, Gingival Status, Malalignment Index, Malocclusion, Permanent Tooth Occlusion Stage 2.

THE EFFECTIVENESS OF THE ROLL TOOTH-BRUSHING TECHNIQUE TOWARD GINGIVAL HEALTH STATUS OF TOTALLY BLIND PEOPLE Jessica Laurent Citradi; Isnur Hatta; Aulia Azizah
Dentino : Jurnal Kedokteran Gigi Vol 7, No 1 (2022)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v7i1.13094

Background: Gingivitis is an inflammatory reaction that occurs in gingival tissue. Based on 2018 RISKESDAS data, gingivitis case that occurs in Indonesia reaches 13,9%. The prevalence of gingivitis in South Kalimantan is higher than Indonesia’s, that is 14,2%. The bad habit of mantaining oral health causes plaque accumulation on the marginal gingiva, which resulting in gingivitis. The said condition also affects disabled people, one of which is blind people. Tooth brushing intervention can be done in various kind. One of the recommended methods is the roll technique that effectively cleans plaque and can mantain gum health. Purpose: To analyze the effectiveness of the roll tooth-brushing technique toward the gingival health status (gingival index) of totally blind people. Methods: This research used quasi-experimental research method with non-equivalent control group design. The sampling technique was using purposive sampling with total samples were 15 people. This research was conducted in 21 days with 9 times encounter. The time span of each encounter is once in every 2 days. Gingival index examination used gingival index. The data analysis in this research was using Mann Whitney Test. Result: The Mann Whitney test result shows that p-value is 0,018, which is smaller than the alpha value that is 0,05 (p<0,05) which means that there is a difference between the case group that was given with socialization and roll brushing technique and the control group that was not given the said socialization and treatment. Conclusion: The roll tooth-brushing technique is effective for gingival health status (gingival index) of totally blind people.

HUBUNGAN KECEMASAN DENTAL TERHADAP PERFORMANCE TREATMENT INDEX PADA ANAK KELAS 5-6 SDN BERANGAS TIMUR 1 KECAMATAN ALALAK KABUPATEN BARITO KUALA Muhammad Aulia Rifa Syarafi; Rosihan Adhani; Aulia Azizah
Dentin Vol 5, No 1 (2021)
Publisher : Universitas Lambung Mangkurat

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ABSTRACTBackground: Dental anxiety is cause of psychological symptoms, as depression, fear, and discomfort regarding dental treatment. Prevalence of dental anxiety from population in several countries mostly in children with rates 5-20%. Feelings fear dental care become obstacle for dentists their efforts improve public dental health. This condition affects Performance Treatment Index, which is percentage number of teeth still filled with DMF-T number. PTI describes person's motivation to fill cavities in effort maintain permanent teeth. Based Riskesdas data, PTI in Barito Kuala is only 0.48%. Purpose: Analyzing relationship between dental anxiety Performance Treatment Index in children5-6 at SDN Berangas Timur 1, Barito Kuala Regency. Methods:This study used analytic observational study with cross sectional approach to 28 respondents. Research instrument for measuring anxiety using CFSS-DS questionnaire and PTI measurement using DMF-T index then calculated using PTI formula. Results: Research found results of dental anxiety with 22 children not anxious and 6 children anxious. Results with the good PTI category obtained 1 child and poor 27 children. Result Spearman correlation test shows value p = 0.611. Conclusion: There was no relationship between dental anxiety and Performance Treatment Index in children at SDN Berangas Timur 1, Alalak District, Barito Kuala Regency.Keywords: Children, Dental Anxiety, Performance Treatment Index.ABSTRAKLatar Belakang: Kecemasan dental adalah penyebab dari gejala psikologis, seperti depresi, ketakutan, dan perasaan tidak nyaman terhadap perawatan dental. Prevalensi kecemasan dental dari populasi pada beberapa negara paling banyak pada anak-anak dengan angka 5-20%. Perasaan takut terhadap perawatan gigi menjadi hambatan dokter gigi dalam usaha peningkatan kesehatan gigi masyarakat. Kondisi ini berpengaruh pada Performance Treatment Index, yaitu presentase jumlah gigi tetap ditambal terhadap angka DMF-T. PTI menggambarkan motivasi seseorang untuk menambalkan gigi berlubang dalam upaya mempertahankan gigi tetap. Berdasarkan data Riskesdas, PTI di Barito Kuala hanya 0,48%. Tujuan: Menganalisis hubungan antara Kecemasan dental terhadap Performance Treatment Index pada anak-anak Kelas 5-6 di SDN Berangas Timur 1 Kecamatan Alalak. Metode: Penelitian ini menggunakan penelitian observasional analitik dengan pendekatan Cross Sectional pada 28 responden. Instrumen penelitian pada pengukuran kecemasan menggunakan kuesioner CFSS-DS dan pengukuran PTI menggunakan indeks DMF-T kemudian dihitung menggunakan rumus PTI. Hasil: Penelitian ini mendapatkan hasil kecemasan dental dengan 22 anak tidak cemas dan 6 anak cemas. Hasil dengan kategori PTI baik diperoleh 1 anak dan buruk 27 anak. Hasil uji korelasi Spearman menunjukkan nilai p=0,611. Kesimpulan: Tidak terdapat hubungan antara Kecemasan dental terhadap Performance Treatment Index pada anak-anak di SDN Berangas Timur 1 Kecamatan Alalak Kabupaten Barito Kuala.Kata kunci: Anak-anak, Kecemasan dental, Performance Treatment Index

EFEKTIVITAS PENYULUHAN MENGGUNAKAN LAGU “GIGI SEHAT” TERHADAP PENURUNAN PLAK DI BARITO KUALA (Tinjauan SDN Barangas Timur 1 Kecamatan Alalak Kabupaten Barito Kuala) Faulina Windiyana; Rosihan Adhani; Aulia Azizah
Dentin Vol 4, No 1 (2020)
Publisher : Universitas Lambung Mangkurat

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ABSTRAK Background: Basic Health Research Results (Riskesdas) in 2018 showed the dental and oral health problems of the people in Indonesia reached 57.6%, while the dental and mouth problems in the province of South Kalimantan in children aged 5-9 years amounted to 71.17%, the daily brushing behavior amounted to 96.04% but the correct brushing time is only 4.97%. This indicates that there is still a lack of knowledge about how and when to brush the teeth properly and correctly so it needs an innovation in providing information in this is necessary. The counseling by using the songs is expected to change a person's behavior in ways and when they’re brushing their teeth properly. Objective: To analyze the effectiveness of the counseling using the "Healthy Teeth" song in students at the East Java 1 East Elementary School, Alalak District, Barito Kuala Regency. Method: Using an experimental and using the non equivalent control group design by dividing the respondents into two groups. The research was conducted at Barangas Blanket 1 Elementary School in Alalak Sub-district, Barito Kuala District, using the technique of probability sampling with positive sampling of 38 respondents. Results: The Whitney Mann test was found to have a significant significance of 0.018 <0.05, meaning that the effectiveness of using the "Healthy Teeth" song indeed decrease the index. Conclusion: Counseling using "Healthy Teeth" songs has effective effect in reducing plaque.Keywords: Counseling, Dental Plaque, SongABSTRAK Latar belakang: Hasil Riset Kesehatan Dasar (Riskesdas) tahun 2018, masalah kesehatan gigi dan mulut masyarakat Indonesia sebesar 57,6%, sedangkan masalah gigi dan mulut di Provinsi Kalimantan selatan pada anak usia 5-9 tahun sebesar 71,17%, perilaku menyikat gigi setiap hari sebesar 96,04% tetapi waktu menyikat gigi yang benar hanya sebesar 4,97%. Hal tersebut menandakan bahwa masih kurangnya pengetahuan tentang cara dan waktu menyikat gigi yang baik dan benar sehingga perlunya suatu inovasi dalam memberikan informasi dalam hal tersebut. Penyuluhan menggunakan lagu diharapakan dapat merubah perilaku seseorang dalam cara dan waktu menyikat gigi yang baik dan benar. Tujuan: Menganalisis efektivitas penyuluhan menggunakan lagu “Gigi Sehat” pada siswa di SDN Barangas Timur 1 Kecamatan Alalak Kabupaten Barito Kuala. Metode: Menggunakan quasi eksperimental dengan rancangan non equivalent control grup design dengan membagi responden menjadi dua kelompok. Penelitian ini di laksanakan di SDN Barangas Timut 1 Kecamatan Alalak Kabupaten Barito Kuala dengan teknik pengambilan sampel menggunakan teknik non probability sampling dengan purposive sampling yaitu sebanyak 38 responden. Hasil: Uji Mann Whitney didapatkan nilai signifikansi sebesar 0,018 < 0,05, artinya terdapat efektivitas penyuluhan menggunakan lagu “Gigi Sehat” terhadap penurunan indeks plak. Kesimpulan: Penyuluhan menggunakan lagu “Gigi Sehat” efektif dalam menurunkan plak. Kata kunci:, lagu, penyuluhan, plak

HUBUNGAN ANTARA KEBIASAAN BURUK BERNAFAS MELALUI MULUT DENGAN TINGKAT KEPARAHAN MALOKLUSI PADA ANAK SEKOLAH DASAR Dinda Chesya; Diana Wibowo; Aulia Azizah
Dentin Vol 5, No 3 (2021)
Publisher : Universitas Lambung Mangkurat

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ABSTRACTBackground: In Indonesia, malocclusion is a very high dental and oral health problem, accounting for about 80% of the population, ranks third after dental caries and periodontal disease. According to Basic Health Research (Riskesdas) data, South Kalimantan is an area with a high incidence of dental and oral health problems, which is around 59.6%, and cases of malocclusion in South Kalimantan are around 12%. This habit is most often done in elementary school children. Elementary school children aged 6-12 years need early diagnosis, because the permanent and primary teeth of this age group are fused in the oral cavity, and the occlusion is still temporary. If a malocclusion is found, it is easier to treat. Purpose: The purpose of the literature study was to determine the relationship between bad mouth breathing habits and the severity of malocclusion in elementary school children. Methods: All reviewed articles were obtained from searching Google Scholar, Pubmed and Science Direct data sources which have a maximum journal publication time of 10 years. Results: Bad habit of breathing through the mouth in elementary school children has a high percentage of 64.52%, and in children who breathe through the nose is 35.48%. The severity of malocclusion in children who have bad habits of mouth breathing is moderate malocclusion. The most cases of malocclusion are cases of class II division 1. Conclusion: There is a relationship between bad mouth breathing habits and the severity of malocclusion in elementary school children. Keywords: Bad habits, mouth breathing, malocclusion ABSTRAKLatar belakang : Di Indonesia, maloklusi merupakan masalah kesehatan gigi dan mulut yang sangat tinggi, terhitung sekitar 80% dari populasi, menempati urutan ketiga setelah karies gigi dan penyakit periodontal. Menurut data Riset Kesehatan Dasar (Riskesdas), Kalimantan Selatan merupakan daerah dengan insiden masalah kesehatan gigi dan mulut yang tinggi yaitu sekitar 59,6%, dan kasus maloklusi yang terjadi di Kalimantan Selatan sekitar 12%. Kebiasaan ini paling sering dilakukan pada anak sekolah dasar. Anak SD usia 6-12 tahun perlu diagnosis dini, karena gigi tetap dan gigi sulung kelompok usia ini menyatu dalam rongga mulut, dan oklusinya masih bersifat sementara. Jika ditemukan maloklusi, lebih mudah untuk dirawat. Tujuan: Tujuan studi literature untuk mengetahui hubungan antara kebiasaan buruk bernafas melalui mulut dengan tingkat keparahan maloklusi pada anak sekolah dasar. Metode: Semua artikel yang direview diperoleh dari pencarian sumber data Google Scholar, Pubmed dan Science Direct yang memiliki rentang waktu penerbitan jurnal maksimal 10 tahun terakhir. Hasil: Kebiasaan buruk bernafas melalui mulut pada anak sekolah dasar memiliki persentase tinggi yaitu 64,52%, dan pada anak yang bernafas melalui hidung yaitu 35,48%. Tingkat keparahan maloklusi pada anak yang memiliki kebiasaan buruk bernafas melalui mulut yaitu maloklusi tingkat sedang. Kasus maloklusi yang paling banyak adalah kasus klas II divisi 1. Kesimpulan: Terdapat hubungan antara kebiasaan buruk bernafas melalui mulut dengan tingkat keparahan maloklusi pada anak sekolah dasar. Kata kunci: Bernafas melalui mulut, kebiasaan buruk, maloklusi

PENGARUH MINUMAN ENERGI DAN SUSU FERMENTASI TERHADAP PELEPASAN ION FOSFAT DAN FLUOR RESIN KOMPOSIT BIOAKTIF Irpansyah Irpansyah; Sherli Diana; Aulia Azizah
Dentin Vol 4, No 3 (2020)
Publisher : Universitas Lambung Mangkurat

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ABSTRACTBackground: Bioactive composite resin is a restoration material which has the ability to release calcium, phosphate, and fluorine ions. Bioactive composites release the ion at an acidic pH. The release of ions causes reduced activity of protease enzymes such as metalloproteinases (MMPs) and cathepsins which play a role in collagen degradation. This enzyme is one of the causes of decreasing the life of the bioactive composite resin with dentin. Purpose: The purpose of this study was to determine the effect of energy drinks and fermented milk on the release levels of phosphate ions and fluorine ions of bioactive composite resins. Methods: The bioactive composite resin specimens (ACTIVATM BioACTIVE-Restorative / USA) with a diameter of 15mm and 1mm thick (ISO 4149) were divided into 3 immersion groups. The release of phosphate ions was measured using a UV-VIS spectrophotometer and the release of fluorine ions was measured using a Lutron pH meter. Results: The largest release of phosphate ions occurred in the energy drink immersion group and the largest release of fluorine ions occurred in the fermented milk immersion group. Conclusion: The immersion group of energy drinks and fermented milk had an effect on the release of phosphate ions and fluorine ionsKeywords : Bioactive Composite Resin, Energy Drink, Fermented Milk, Fluorine Ion, Phosphate IonABSTRAK Latar Belakang: Resin komposit bioaktif merupakan bahan restorasi yang memiliki kemampuan melepaskan ion kalsium, ion fosfat, dan ion fluor. Komposit bioaktif mengeluarkan ion pada pH yang asam. Pelepasan ion menyebabkan berkurangnya aktivitas enzim protease seperti metalloproteinases (MMPs) dan cathepsin yang berperan dalam degradasi kolagen. Enzim tersebut merupakan salah satu penyebab penurunan usia ikatan resin komposit bioakif dengan dentin. Tujuan: Tujuan penelitian ini adalah untuk mengetahui pengaruh minuman energi dan susu fermentasi terhadap kadar pelepasan ion fosfat dan ion fluor resin komposit bioaktif. Metode: Spesimen resin komposit bioaktif (ACTIVATM BioACTIVE-Restorative/USA) diameter 15mm dan tebal 1mm (ISO 4149) yang dibagi menjadi 3 kelompok perendaman. Pelepasan ion fosfat diukur menggunakan spektrofotometer UV-VIS dan pelepasan ion fluor diukur menggunakan pH meter Lutron. Hasil: Pelepasan ion fosfat terbesar terjadi pada kelompok perendaman minuman energi dan pelepasan ion fluor terbesar terjadi pada kelompok perendaman susu fermentasi. Kesimpulan: Kelompok perendaman minuman energi dan susu fermentasi memiliki pengaruh terhadap pelepasan ion fosfat dan ion fluor.Kata kunci: Ion Fluor, Ion Fosfat, Minuman Energi, Resin Komposit bioaktif, Susu Fermentasi.

UJI TOKSISITAS EKSTRAK KULIT BATANG ULIN (Eusideroxylon zwageri) TERHADAP SEL FIBROBLAS BHK-21 SECARA IN VITRO Ismi Natasya Salwa; I Wayan Arya Krishnawan Firdaus; Aulia Azizah
Dentin Vol 5, No 3 (2021)
Publisher : Universitas Lambung Mangkurat

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ABSTRACTBackground: Antioxidants can come from plants that contain flavonoids such as ironwood. The bark of ironwood is declared effective to be used as a mouthwash because of the content of flavonoids, phenolics, tannins, saponins, alkaloids, and terpenoids. Ironwood bark extract to be used as an alternative material must be safe for oral tissues, so it is necessary to do a toxicity test. Objective: To analyze the toxic effect of ironwood bark extract through IC50 on BHK-21 fibroblast cells. Methods: This study was a pure laboratory experimental study with posttest-only control group design, consisting of 12 groups with 10 treatment groups given ironwood bark extract at concentrations of 5%, 15%, 25%, 35%, 45%, 55%. , 65%, 75%, 85%, and 95% and 2 control groups, namely cell control and media control. It was repeated 3 times so that the total sample was 36 samples. Toxicity test media used MTT assay which produced color absorbance and cell viability was calculated. Results: The results showed that the cell viability of the entire treatment group was >60% so it had no toxic effect. In addition, based on the IC50> 0.1%, which is 3.746%, it has no toxic effect on BHK-21 fibroblast cells. Conclusion: Ironwood bark extract at concentrations of 5%, 15%, 25%, 35%, 45%, 55%, 65%, 75%, 85%, and 95% had no toxic effect on BHK-21 fibroblast cells. Keywords: BHK-21 fibroblast cells, Ironwood bark extract, , toxicity ABSTRAKLatar Belakang: Antioksidan dapat berasal dari tanaman yang mengandung flavonoid seperti ulin. Kulit batang ulin dinyatakan efektif untuk dijadikan obat kumur karena adanya kandungan flavonoid, fenolik, tanin, saponin, alkaloid, dan terpenoid. Ekstrak kulit batang ulin untuk dijadikan sebagai bahan alternatif harus bersifat aman untuk jaringan rongga mulut sehingga perlu dilakukan uji toksisitas. Tujuan: Menganalisis efek toksik ekstrak kulit batang ulin melalui IC50 terhadap sel fibroblas BHK-21. Metode: Penelitian eksperimental laboratoris murni dengan posttest-only with control group design, terdiri dari 12 kelompok dengan 10 kelompok perlakuan yang diberi ekstrak kulit batang ulin konsentrasi 5%, 15%, 25%, 35%, 45%, 55%, 65%, 75%, 85%, dan 95% dan 2 kelompok kontrol, yaitu kontrol sel dan kontrol media. Dilakukan 3 kali pengulangan sehingga total sampel berjumlah 36 sampel. Media uji toksisitas menggunakan MTT assay yang menghasilkan absorbansi warna dan dilakukan perhitungan viabilitas sel. Hasil: Viabilitas sel seluruh kelompok perlakuan adalah >60% sehingga tidak memiliki efek toksik. Selain itu, berdasarkan nilai IC50>0,1% yaitu sebesar 3,746% tidak memiliki efek toksik terhadap sel fibroblas BHK-21. Kesimpulan: Ekstrak kulit batang ulin pada konsentrasi 5%, 15%, 25%, 35%, 45%, 55%, 65%, 75%, 85%, dan 95% tidak memiliki efek toksik terhadap sel fibroblas BHK-21. Kata kunci: Esktrak kulit batang ulin, sel fibroblas BHK-21, toksisitas.

HUBUNGAN USIA, JENIS KELAMIN DAN ELEMEN GIGI DENGAN ANGKA KEJADIAN LESI PERIAPIKAL Muhammad Luthfi Azzuhdi; Isyana Erlita; Aulia Azizah
Dentin Vol 5, No 1 (2021)
Publisher : Universitas Lambung Mangkurat

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ABSTRACKBackground: Pulp necrosis is the final stage of pulpitis and is the beginning of periapical lesions. Periapical lesions that result from pulp necrosis are acute apical periodontitis, periapical abscess, and chronic apical periodontitis. Age and sex are related to the incidence number of periapical lesions where young people are more susceptible and behavior patterns in sex affect oral hygiene. Molar elements often occur periapical lesions because the molars have deep fit and fissure. Purposes: To determine the incidence number of periapical lesions. To determine the relationship between age and the incidence number of periapical lesions, the relationship between sex and the incidence number of periapical lesions and the relationship between dental elements and the incidence number of periapical lesions. Methods: This study used a descriptive analytic method with a cross-sectional design. The sample used is medical records. The number of samples is 86 with sampling using purposive sampling. Results: The results of the Kolmogorov-Smirnov test for the category of age (0.038<0.05), sex (0.993>0.05) and dental element (0.932>0,05). Conclusion: There is a relationship between age and the incidence number of periapical lesions, but there is no relationship between sex and dental elements and the incidence mumber of periapical lesions.Keywords: Age, dental elements, gender, periapical lesions, pulp necrosis.ABSTRAKLatar Belakang: Nekrosis pulpa merupakan tahap akhir dari pulpitis dan merupakan awal terjadinya lesi periapikal. Lesi periapikal yang terjadi akibat nekrosis pulpa adalah periodontitis apikalis akut, abses periapikal dan periodontitis apikalis kronis. Usia dan jenis kelamin berhubungan dengan angka kejadian lesi periapikal dimana usia muda lebih rentan dan pola perilaku pada jenis kelamin mempengaruhi oral hygiene. Elemen gigi molar sering terjadi lesi periapikal karena gigi molar memiliki fit and fissure yang dalam. Tujuan: Mengetahui angka kejadian lesi periapikal. Mengetahui hubungan antara usia dengan angka kejadian lesi periapikal, hubungan antara jenis kelamin dengan angka kejadian lesi periapikal dan hubungan antara elemen gigi dengan angka kejadian lesi periapikal. Metode: Penelitian ini menggunakan metode deskriptif analitik dengan desain cross-sectional. Sampel yang digunakan adalah rekam medik. Jumlah sampel sebanyak 86 dengan pengambilan sampel menggunakan purposive sampling. Hasil: Hasil uji Kolmogorov-smirnov untuk kategori usia (0.038<0.05), jenis kelamin (0.993>0.05) dan elemen gigi (0.932>0.05). Kesimpulan: Ada hubungan antara usia dengan angka kejadian lesi periapikal, tetapi tidak ada hubungan antara jenis kelamin dan elemen gigi dengan angka kejadian lesi periapikal. Kata kunci:Elemen gigi, jenis kelamin, lesi periapikal, nekrosis pulpa, usia.

UJI TOKSISITAS KITOSAN SISIK IKAN HARUAN (Channa striata) TERHADAP SEL VERO Azwar Fida Maulana; Deby Kania Tri Putri; Aulia Azizah
Dentin Vol 6, No 2 (2022)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v6i2.6390

ABSTRACTBackground: Periodontal disease is one of the oral disease problems that still need attention. These diseases are among the diseases with the second highest number of sufferers after caries. Periodontitis is a multifactorial that causes inflammation of the periodontal tissue. Periodontitis is caused by bacteria that trigger infectious diseases in the supporting tissues of the teeth. Haruan fish scale chitosan has potential as an antimicrobial, because it contains lysozyme enzymes and aminopolysacharida groups that can inhibit microbial growth. Haruan fish scale chitosan in suppressing bacterial growth is due to its positively charged polycation. Objective: To analyze whether haruan fish scale chitosan is toxic at concentrations of 20%, 40%, 75% and 100% against vero cells. Methods: This study used a true experimental type with a post-test-only design with a control group design to test the toxicity of chitosan do vero cell with concentrations 20%, 40%, 75% and 100% using MTT assay method. The study used 4 treatment groups and 2 control groups with 4 repetitions. Data analyzed with probit analyisis. Results: The largest cell viability values were concentrations of 100% and 75% of 100%, 40% of 81.4% and 20% concentration of 68.1%. Chitosan with concentrations of 20%, 40%, 75% and 100% was said to be non-toxic due to cell viability 60%. Value IC50 93103,354 μg/mL > 1000 μg/mL categorized non-toxic. Conclusion: Haruan fish scale chitosan with concentrations of 20%, 40%, 75% and 100% against vero was tested to vero cells was not have toxic effect. Keywords: Chitosan, Toxicity, Vero cells. ABSTRAKLatar Belakang: Penyakit periodontal termasuk penyakit dengan penderita tertinggi kedua setelah karies. Periodontitis merupakan penyakit peradangan jaringan periodontal. Periodontitis disebabkan oleh bakteri pemicu infeksi pada jaringan penyangga gigi. Kitosan sisik ikan haruan memilki potensi sebagai antimikroba, karena mengandung enzim lysosim dan gugus aminopolysacharida yang dapat menghambat pertumbuhan mikroba. Kitosan sisik ikan haruan menekan pertumbuhan bakteri karena memiliki polikation bermuatan positif. Tujuan: Menganalisis apakah kitosan sisik ikan haruan bersifat toksik pada konsentrasi 20%, 40%, 75% dan 100% terhadap sel vero. Metode: Penelitian ini menggunakan jenis true experimental dengan rancangan post test-only with control group design untuk menguji toksisitas kitosan sisik ikan haruan terhadap sel vero demam konsentrasi 20%, 40%, 75% dan 100% dengan metode MTT assay. Penelitian menggunakan 4 kelompok perlakuan dan 2 kelompok kontrol dengan 4 pengulangan. Data dianalisis menggunakan analisis probit. Hasil: Nilai viabilitas sel terbesar adalah konsentrasi 100% dan 75% sebesar 100%, 40% sebesar 81,4% dan konsentrasi 20% sebesar 68,1%. Kitosan dengan konsentrasi 20%, 40%, 75% dan 100% dikatakan tidak toksik dikarenakan viabilitas sel ≥ 60%. Nilai IC50 93103,354 μg/mL > 1000 μg/mL termasuk kategori tidak toksik. Kesimpulan: Kitosan sisik ikan haruan dengan konsentrasi 20%, 40%, 75% dan 100% yang diujikan terhadap sel vero tidak memiliki sifat toksik. Kata kunci: Kitosan, Sel Vero, Toksisitas.

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