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PERBEDAAN KEKERASAN PERMUKAAN GIGI AKIBAT LAMA PERENDAMAN DENGAN JUS JERUK (Citrus sinensis. Osb) SECARA IN VITRO Syahrial, Aizar Agi; Rahmadi, Priyawan; Tri Putri, Deby Kania
Dentino Vol 1, No 1 (2016)
Publisher : FKG Unlam

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ABSTRACK Background: Acidic drinks that cause erosion of the teeth. If the acid continue sticking to the teeth it will decrease the surface hardness of tooth. The demineralization of the tooth surface cause the formation of porosity. Purpose: This study aimed to determine the differences in Â urface hardness of tooth due to duration of immersion with orange juice in vitro. Methods: This research design was a true experimental research postest only with control group design. The sample was consisted of 4 treatment groups, one group of teeth that was not immersedwith orange juice as a positive control group and 3 another groups teeth immersedwith orange juice for 30, 60 and 120 minutes. Each tooth surface hardness was measured using the Micro Vicker Hardness Tester. Result: The average hardness of the tooth surface that was not immersed orange juice 372.8 VHN, immersedin orange juice for 30 minutes 323.7 VHN, 60 minutes 292.5 VHN, 120 minutes 259.1 VHN. One way ANOVA test showed significant differences in the surface hardness of the tooth between 2 groups.Conclusion: The longer of immersed time may effect in enamel surface hardness decreases.Â ABSTRAK Latar Belakang: Jenis minuman yang bersifat asam dapat menyebabkan erosi pada gigi. Jika asam terus menempel pada gigi maka akan mengakibatkan penurunan kekerasan permukaan gigi, ini dikarenakan demineralisasi pada permukaan gigi sehingga menyebabkan terbentuknya porositas pada enamel. Tujuan: Penelitian ini bertujuan untuk mengetahui perbedaan kekerasan permukaan gigi akibat lama perendaman dengan jus jeruk secara invitro. Metode: Jenis penelitian ini adalah penelitian eksperimental murni dengan rancangan penelitian postest only with control group design, menggunakan rancangan acak sederhana, sampel terdiri dari 4 kelompok perlakuan, yaitu 1 kelompok gigi yang tidak direndam jus jeruk sebagai kontrol positif dan 3 kelompok gigi yang direndam jus jeruk selama 30, 60 dan 120 menit. Kekerasan permukaan gigi masing â masing diukur kekerasannya menggunakan Micro Vicker Hardness Tester. Hasil: Rata - rata kekerasan permukaan gigi yang tidak direndam jus jeruk 372,8 VHN, direndam jus jeruk selama 30 menit 323,7 VHN, 60 menit 292,5 VHN dan 120 menit 259,1 VHN. Uji one way anova menunjukkan perbedaan bermakna antara kekerasan permukaan gigi yang tidak direndam dan direndam selama 30, 60 dan 120 menit jus jeruk.Kesimpulan: Semakin lama waktu perendaman dapat mengakibatkan kekerasan permukaan enamel semakin menurun.

TOTAL COLONY CHARACTERIZATION OF AEROBIC BACTERIA OF THE ORAL CAVITY ON PEATLANDS WATER AND PDAM WATER Senggara, Bayu Yordha; Tri Putri, Deby Kania; Aspriyanto, Didit
Dentino Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar

Background: South Kalimantan peatland water is approximately 8000 km2. Barito Kuala has 40,858 hectares or 90% of the area classified as peatland water. Society uses peatlands water with acidic pH(3.5-4.5) for everyday purposes including rinsing and brushing teeth. The acidic nature of peatland water can affect the growth of bacterial colonies in the oral cavity so that the acidic conditions of the oral cavity increases and the effect on the occurrence of dental caries. In addition PDAM water is water from river water that is processed through several stages to become clean water after filtration and disinfection stages to eliminate microorganisms. Objective: this study aims to determine the characterization of the number of colonies of aerobic bacteria of the oral cavity on peatland water and PDAM water. Methods: This study used quasi experimental method with post test only control group design. The study sample consisted of 30 respondents. Research materials were 4ml of a result of the salivary gargle on peatland water and PDAM water then the number of aerobic bacteria colonies were calculated by the method of TPC (Total Plate Count) and characterization of aerobic bacteria was done by using catalase test, test MSA and coagulase test. Results: This study showed that there were 1653 CFU/ml of aerobic aerobic colony, whereas in water of PDAM there were 1264 CFU/ml of aerobic colonies. In the characterization tests of aerobic bacteria colonies showed that there were Staphylococcus aureus and Staphylococcus epidermidis bacteria on gargle water of peatland or PDAM water. Independent t-test results showed significant differences in aerobic bacterial colonies in peatland water and PDAM water (p = 0.001)(p = 0.002)(p <0.05). Conclusion: Based on this study it can be concluded that the number of Staphylococcus aureus and Staphylococcus epidermidis found in the oral cavity of children saliva who gargled with peatland water are higher than the number of Stapylococcus aureus and Staphylococcus epidermidis found in the saliva of children who gargle with PDAM water.

Hydroxyl Radical Scavenging Activity of Stachytarpheta jamaecensis Root Extract using In Vitro Deoxyribose Degradation Assay Juliyatin Putri Utami; Yusrinie Wasiaturrahmah; Deby Kania Tri Putri
Majalah Obat Tradisional Vol 26, No 2 (2021)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.61746

Use and demand of traditional medicinal plants currently growing in the direction that research in traditional medicines have also increased. One of the plants that is efficacious as a medicine is S. jamaecensis. This plant is usually used by people as a traditional medicine to treat various diseases. This study aims to determine the antioxidant activity of porterweed root. Antioxidant activity assays performed using hydroxyl radical scavenger that begins with the extraction by maceration. Dry roots of S. jamaecensis (250 g) was soaked with ethanol 96% for 3x24 hours at room temperature. The liquid extract obtained is evaporated by rotary evaporator and waterbath till viscous extracts is obtained. Then to extract performed phytochemical screening by quantitative and qualitative method. The antioxidant test was carried out using the hydroxyl radical scavenger method. The extract concentrations were varied, namely 125, 250, 500 and 1000 ppm. The data obtained was performed one-way ANOVA test. The results of the phytochemical screening showed the extract contains flavonoids, alcaloids, saponins, phenols and tannins. The results obtained were significant values from the antioxidant test with the antiradical scavenger method, the largest average value of root extract was 60.606% at a concentration of 1000 ppm. The results of spectrophotometric measurements showed that the extract has IC50 was 683.5294 µg/ml, while vitamin C has IC50 lower value (251.700 µg/ml). Q

ANTIBACTERIAL ACTIVITY OF CHITOSAN FROM HARUAN (Channa striata) FISH SCALES AGAINST THE GROWTH OF Porphyromonas gingivalis Rahma Dania A.J; Deby Kania Tri Putri; Irham Taufiqurrahman
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8122

Background: Periodontal disease is one of the dental and oral diseases that is often found in people in Indonesia. RISKESDAS (2018) stated that the prevalence of people who have dental and oral health problems in Indonesia is 57.6%. The prevalence of oral and dental problems in South Kalimantan is 60%. Periodontal disease is damage to the supporting tissues of the teeth caused by specific microorganisms such as Porphyromonas gingivalis which are gram negative bacteria. The growth of these bacteria can be inhibited and killed by chitosan from haruan fish scales. Chitosan is a natural product of chitin, chitosan is the basic ingredient of fish scales. Chitosan has advantages and more safety levels, because it has an active group that will bind to microbes so chitosan can inhibit and kill microbial growth. Objective: to analyze the antibacterial activity of chitosan scales (Channa striata) on the growth of Porphyromonas gingivalis. Methods: This research used true experimental with randomized pre and post test with only control group design with eight treatments. Chitosan of haruan fish scales with liquid dilution method to obtain MIC and solid dilution test to obtain MBC. Results: The results showed that Minimum Inhibitory Concentration (MIC) of chitosan haruan scales was found at a concentration of 2.5% characterized by a decrease in absorbance value of 0.092 and Minimum Bactericidal Concentration found at a concentration of 20% characterized by the absence of Porphyromonas gingivalis. Conclusion: There is an inhibitory activity and a bactericidal activity from chitosan of haruan fish scales on Porphyromonas gingivalis.

ANTIBACTERIAL ACTIVITIES OF CHITOSAN IN HARUAN FISH SCALES (Channa striata) TO THE GROWTH OF Staphylococcus aureus Dewi Retno Wahyu Widyaningrum; Deby Kania Tri Putri; Irham Taufiqurrahman
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7045

Background: Haruan fish scales (Channa striata) contain chitin which can be used as antibacterial ingredients by processing it into chitosan. Chitosan contains an amino polysaccharide group which can inhibit the growth of Staphylococcus aureus, one of the opportunistic bacteria, which becomes a pathogen if there is an imbalance within oral environment and one of the causes of periodontal disease. Besides, these bacteria are also reported to be resistant to conventional antibacterial agents. Objective: The purpose of this study was to find out and elaborate the difference in antibacterial activity of chitosan from haruan fish scales (Channa striata) at the concentration of 0.5%; 1%; 1.5%; 2%; 2.5%; 3%; 3.5%; 4% on the growth of Staphylococcus aureus. Methods: This study used a randomized pretest-posttest with control group design and One Way ANOVA statistical test was performed and was followed by Post Hoc Dunnet test. The procedure of this study consisted of chitosan fabrication scales from fresh fish by deproteination, demineralization, and deacetylation, followed by an anti-bacterial test with 10 treatment groups namely 0.5%; 1%; 1.5%; 2%; 2.5%; 3%; 3.5%; 4% on the growth of Staphylococcus aureus to obtain Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results: The results of this study presented chitosan at 1.5% concentration as the Minimum Inhibitory Concentration (MIC) and 3.5% concentration as the Minimum Bactericidal Concentration (MBC) on the growth of Staphylococcus aureus. Evaluated from the difference in absorbance, 2.5% chitosan concentration showed superior activity in inhibiting Staphylococcus aureus compared to 0.2% chlorhexidine gluconate. Conclusion: It can be concluded that there are differences in the antibacterial activity of chitosan scales (Channa striata) with a concentration of 0, 5%; 1%; 1.5%; 2%; 2.5%; 3%; 3.5%; 4% on the growth of Staphylococcus aureus.

TOTAL COLONY CHARACTERIZATION OF AEROBIC BACTERIA OF THE ORAL CAVITY ON PEATLANDS WATER AND PDAM WATER Bayu Yordha Senggara; Deby Kania Tri Putri; Didit Aspriyanto
Dentino : Jurnal Kedokteran Gigi Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i1.4619

Background: South Kalimantan peatland water is approximately 8000 km2. Barito Kuala has 40,858 hectares or 90% of the area classified as peatland water. Society uses peatlands water with acidic pH(3.5-4.5) for everyday purposes including rinsing and brushing teeth. The acidic nature of peatland water can affect the growth of bacterial colonies in the oral cavity so that the acidic conditions of the oral cavity increases and the effect on the occurrence of dental caries. In addition PDAM water is water from river water that is processed through several stages to become clean water after filtration and disinfection stages to eliminate microorganisms. Objective: this study aims to determine the characterization of the number of colonies of aerobic bacteria of the oral cavity on peatland water and PDAM water. Methods: This study used quasi experimental method with post test only control group design. The study sample consisted of 30 respondents. Research materials were 4ml of a result of the salivary gargle on peatland water and PDAM water then the number of aerobic bacteria colonies were calculated by the method of TPC (Total Plate Count) and characterization of aerobic bacteria was done by using catalase test, test MSA and coagulase test. Results: This study showed that there were 1653 CFU/ml of aerobic aerobic colony, whereas in water of PDAM there were 1264 CFU/ml of aerobic colonies. In the characterization tests of aerobic bacteria colonies showed that there were Staphylococcus aureus and Staphylococcus epidermidis bacteria on gargle water of peatland or PDAM water. Independent t-test results showed significant differences in aerobic bacterial colonies in peatland water and PDAM water (p = 0.001)(p = 0.002)(p <0.05). Conclusion: Based on this study it can be concluded that the number of Staphylococcus aureus and Staphylococcus epidermidis found in the oral cavity of children saliva who gargled with peatland water are higher than the number of Stapylococcus aureus and Staphylococcus epidermidis found in the saliva of children who gargle with PDAM water.

PERBEDAAN KEKERASAN PERMUKAAN GIGI AKIBAT LAMA PERENDAMAN DENGAN JUS JERUK (Citrus sinensis. Osb) SECARA IN VITRO Aizar Agi Syahrial; Priyawan Rahmadi; Deby Kania Tri Putri
Dentino : Jurnal Kedokteran Gigi Vol 1, No 1 (2016)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v1i1.406

ABSTRACK Background: Acidic drinks that cause erosion of the teeth. If the acid continue sticking to the teeth it will decrease the surface hardness of tooth. The demineralization of the tooth surface cause the formation of porosity. Purpose: This study aimed to determine the differences in urface hardness of tooth due to duration of immersion with orange juice in vitro. Methods: This research design was a true experimental research postest only with control group design. The sample was consisted of 4 treatment groups, one group of teeth that was not immersedwith orange juice as a positive control group and 3 another groups teeth immersedwith orange juice for 30, 60 and 120 minutes. Each tooth surface hardness was measured using the Micro Vicker Hardness Tester. Result: The average hardness of the tooth surface that was not immersed orange juice 372.8 VHN, immersedin orange juice for 30 minutes 323.7 VHN, 60 minutes 292.5 VHN, 120 minutes 259.1 VHN. One way ANOVA test showed significant differences in the surface hardness of the tooth between 2 groups.Conclusion: The longer of immersed time may effect in enamel surface hardness decreases. ABSTRAK Latar Belakang: Jenis minuman yang bersifat asam dapat menyebabkan erosi pada gigi. Jika asam terus menempel pada gigi maka akan mengakibatkan penurunan kekerasan permukaan gigi, ini dikarenakan demineralisasi pada permukaan gigi sehingga menyebabkan terbentuknya porositas pada enamel. Tujuan: Penelitian ini bertujuan untuk mengetahui perbedaan kekerasan permukaan gigi akibat lama perendaman dengan jus jeruk secara invitro. Metode: Jenis penelitian ini adalah penelitian eksperimental murni dengan rancangan penelitian postest only with control group design, menggunakan rancangan acak sederhana, sampel terdiri dari 4 kelompok perlakuan, yaitu 1 kelompok gigi yang tidak direndam jus jeruk sebagai kontrol positif dan 3 kelompok gigi yang direndam jus jeruk selama 30, 60 dan 120 menit. Kekerasan permukaan gigi masing – masing diukur kekerasannya menggunakan Micro Vicker Hardness Tester. Hasil: Rata - rata kekerasan permukaan gigi yang tidak direndam jus jeruk 372,8 VHN, direndam jus jeruk selama 30 menit 323,7 VHN, 60 menit 292,5 VHN dan 120 menit 259,1 VHN. Uji one way anova menunjukkan perbedaan bermakna antara kekerasan permukaan gigi yang tidak direndam dan direndam selama 30, 60 dan 120 menit jus jeruk.Kesimpulan: Semakin lama waktu perendaman dapat mengakibatkan kekerasan permukaan enamel semakin menurun.

EFFECTIVENESS OF KARAMUNTING FLOWER EXTRACT (Melastoma malabathricum L) ON THE STREPTOCOCCUS MUTANS GLUCOSYLTRANSFERASE ENZYME ACTIVITY Muhammad Zainal Fikri; Deby Kania Tri Putri; Muhammad Yanuar Ichrom Nahzi
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7046

Background: Caries is a chronic disease of mineral ions continuous loss caused by bacteria in oral cavity. One of the main bacteria is Streptococcus mutans. This bacterium is able to synthesize the glucosyltransferase enzyme (GTF), which function is to catalyze polysaccharides in the form of sucrose into sticky glucans. This pathogenic property also has function in helping bacteria to stick and accumulate on the tooth surface, so it can increase the occurrence of the demineralization process and dental caries. Karamunting flower extract, as a natural ingredient, has antibacterial property. This property is caused by the content of phenol, flavonoid and tannin, which can reduce the GTF S. mutans enzyme activity. Objectives: This study aimed to analyze the effectiveness of karamunting flower extract (Melastoma malabathricum L) on the activity of the Stretococcus mutans glucosyltransferase enzyme. Methods: This study used a post-test only with control group design with karamunting flower extract samples in various concentration, including 40%, 50%, 60%, 70%, measuring enzyme activity with 4 repetitions in each group. Results: The data were analyzed using One Way ANOVA, which got p = 0,000 (p <0.05) and followed by Post Hoc LSD test that showed significant difference between each group (p <0.05). Data showed that concentrations of 40%, 50%, 60%, and 70% can reduce GTF S. mutans enzyme activity. Conclusion: Karamunting flower extract has the effectiveness of reducing the GTF enzyme activity of S. mutans.

EFFECT OF KARAMUNTING LEAF EXTRACT (Melastoma malabathricum L.) ON GLUCOSYLTRANSFERASE ENZYME OF Streptococcus mutans Ferdy Juliannor Fajar; Deby Kania Tri Putri; Bayu Indra Sukmana
Dentino : Jurnal Kedokteran Gigi Vol 5, No 2 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i2.8946

ABSTRACTBackground: Karamunting leaf extract has secondary metabolite compounds such as tannin, flavonoid, and phenol that have antibacteria potential to be used as an alternative to mouthwash in addition to Chlorhexidine 0.12% to lower the incidence of tooth caries. Caries is caused by several important virulence factors, including Streptococcus mutans Glucosyltransferase (GTF) enzyme. Karamunting leaf extract can inhibit the work of GTF enzyme by precipitating, denaturing protein and damaging cell walls. Inhibition of Streptococcus bacteria enzyme activity will decrease plaque formation, thus decreasing the potential of dental caries. Objective: The purpose of this study was to determine the effect of Karamunting leaf extract and 0,12% Chlorhexidine on Glucosyltransferase enzyme activity of Streptococcus mutans. Methods: This study used true experimental design with post test only with control group design using 7 groups, including Karamunting leaf extract group with 5%, 10%, 15%, 20%, and 25% concentrations to determine the activity of S. mutans GTF enzyme. Results: The results showed that Karamunting leaf extract with 20% concentration was able to decrease the activity of Glucosyltransferase enzyme in Streptococcus mutans. Conclusion: Karamunting leaf extract affects Streptococcus mutans Glucosyltransferase enzyme activity. Keywords: Chlorhexidine 0,12%, Glucosiltransferase, Melastoma malabathricum L., Streptococcus mutans.

ANTIOXIDANT ACTIVITY POTENCY OF CHITOSAN FROM HARUAN (CHANNA STRIATA) SCALES Deby Kania Tri Putri; Beta Widya Oktiani; Candra Candra; Rosihan Adhani
Dentino : Jurnal Kedokteran Gigi Vol 5, No 2 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i2.8951

ABSTRACTBackground: Stimulation of inflammatory cells such as polymorphonuclear cells (PMN) will initiate to release free radicals in destroying bacteria. Excessive free radicals can damage cells in the body. Antioxidants as one of the body's defense systems will neutralize existing free radicals. Chitosan from Haruan scales in addition to being antimicrobial turned out to also be antioxidants. Objective: To know the antioxidant potential of chitosan from haruan (Channa striata) scales. Methods: This study is a pure experimental (true experimental design) with a post-test only with control group design using two treatment groups, with quantitative tests to calculate the amount of antioxidant activity of chitosan from fish scales using DPPH radical reduction method. The first treatment is 4, 6, 8 and 10 ppm standard ascorbic acid concentration with DPPH solution as positive control, and the second treatment is 200, 250, 300 and 350 ppm chitosan from Haruan scales concentration with DPPH solution. Results: The results showed that chitosan from Haruan fish scales have antioxidant activity with 50.513% percentage of inhibition at a maximum concentration of 350 ppm with an IC50 value of 356.98 ppm. The results of the independent T-test showed that there was a significant difference between the inhibition percentage of chitosan from haruan fish scales and the inhibition percentage of ascorbic acid (p = 0,000) (p <0.05). Conclusion: Chitosan from Haruan fish scales proved to have antioxidant activity.Keywords: Antioxidants, Chitosan, Haruan fish scalesABSTRACTBackground: Stimulation of inflammatory cells such as polymorphonuclear cells (PMN) will initiate to release free radicals in destroying bacteria. Excessive free radicals can damage cells in the body. Antioxidants as one of the body's defense systems will neutralize existing free radicals. Chitosan from Haruan scales in addition to being antimicrobial turned out to also be antioxidants. Objective: To know the antioxidant potential of chitosan from haruan (Channa striata) scales. Methods: This study is a pure experimental (true experimental design) with a post-test only with control group design using two treatment groups, with quantitative tests to calculate the amount of antioxidant activity of chitosan from fish scales using DPPH radical reduction method. The first treatment is 4, 6, 8 and 10 ppm standard ascorbic acid concentration with DPPH solution as positive control, and the second treatment is 200, 250, 300 and 350 ppm chitosan from Haruan scales concentration with DPPH solution. Results: The results showed that chitosan from Haruan fish scales have antioxidant activity with 50.513% percentage of inhibition at a maximum concentration of 350 ppm with an IC50 value of 356.98 ppm. The results of the independent T-test showed that there was a significant difference between the inhibition percentage of chitosan from haruan fish scales and the inhibition percentage of ascorbic acid (p = 0,000) (p <0.05). Conclusion: Chitosan from Haruan fish scales proved to have antioxidant activity. Keywords: Antioxidants, Chitosan, Haruan fish scales.

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