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All Journal Jurnal Kedokteran Syiah Kuala JURNAL WIDYA MEDIKA Berkala Ilmu Kesehatan Kulit dan Kelamin Journal Of Vocational Health Studies Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Layanan Masyarakat (Journal of Public Service) CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL Indian Journal of Forensic Medicine & Toxicology Jurnal ABM Mengabdi Jurnal Ilmiah Kesehatan (JIKA)
ManikRetno Wahyunitisari
Departement Of Medical Microbiology, Faculty Of Medicine, Airlangga University/Dr. Soetomo General Hospital Surabaya
Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Analysis of Staphylococcus aureus and Escherichia Coli and its Susceptibility to Antibiotic in Catheter-Associated Urinary Tract Infection Patients at Hospital in Province of West Nusa Tenggara BaiqIsti Hijriani; ManikRetno Wahyunitisari; Agung Dwi Wahyu Widodo
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 2 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i2.17945

Abstract

Objective – This study aimed to analyze the proportion of Staphylococcus aureus and Escherichia colifrom urine samples of catheter-associated urinary tract infections (CA-UTI) patients at the GeneralHospital at Province of West Nusa Tenggara. Also measured the pattern of sensitivity to severalantibiotics.Methods –This study is a descriptive observational with a cross-sectional approach. A total of 60 sampleswere used in this study. Bacterial identification was carried out according to standard bacteriologicalculture techniques. Furthermore, the antibiotic sensitivity test following the Kirby-Bauer disc diffusionmethod using several antibiotics, including amoxycillin (AMP), ciprofloxacin (CIP), ceftriaxone(CRO), and sulphamethoxazole trimethoprim (SXT).Results –The results showed that bacteria causing CA-UTI were Staphylococcus aureus (43.33%),Escherichia coli (21.67%), Staphylococcus epidermidis (10%), Proteus mirabilis (6.67%), Enterobacteraerogenes (6.67%), Serratica marcescens (5%), Klebsiella sp (3.33%), and Pseudomonas sp (3.33%).The antibiotic susceptibility test found that 4.58%, 11.25%, 9.16%, and 3% of bacteria were resistant toamoxycillin, ceftriaxone, ciprofloxacin, and sulphamethoxazole trimethoprim, respectively.Conclusion –Staphylococcus aureus and Escherichia coli were the most gram-positive and gramnegativebacteria causing CA-UTI, respectively. The most resistant antibiotic was ciprofloxacin, andthe most sensitive antibiotic is sulphamethoxazole trimethoprim.
Progress In Knowledge Of Microscopic Tuberculosis Diagnosis Laboratory Training Participants Silvia Sutandhio; Ni Made Mertaniasih; Eko Budi Koendhori; Deby Kusumaningrum; Titiek Sulistyowati; Lindawati Alimsardjono; Pepy Dwi Endraswari; Manik Retno Wahyunitisari; Rebekah Juniati Setiabudi
JURNAL WIDYA MEDIKA Vol 5, No 2 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (366.576 KB) | DOI: 10.33508/jwm.v5i2.2202

Abstract

Indonesia is the second highest tuberculosis (TB)-burden country in the world. TB affects men and women in productive ages. Early and accurate diagnosis of TB is crucial in TB management because it allows prompt treatment with appropriate regimen. Sputum smear microscopy is the most common method used in resource-limited laboratories. The aim of this study is to assess whether adult learning method with student-centered approach can improve knowledge in laboratory techniques in microscopic TB diagnosis. It was a quasi experimental research with one group pretest and posttest design. Pretest was conducted before the training started. During the training, participants attended lectures, discussion sessions, and hands-on laboratory activities. After training, participants were asked to respond to posttest. There were 33 participants of this training (n=33). Paired t-test analysis showed significant difference between pretest and posttest scores (p=6.5x10-9). Variance between pretest scores was 4.00 and variance between posttest scores were 1.34. Pearson correlation coefficient was 0.45. Regardless their level of knowledge before training, participants could achieve relatively equivalent results after training. Significant difference between pre- and post-training test scores indicated that the learning method was effective and the training could improve participants knowledge in laboratory techniques in microscopic TB diagnosis.
Ence Of ESBL Bacteria In Baby Box Handle At Dr. Soetomo Hospital Manik Retno Wahyunitisari; Martono Tri Utomo; Nicholas Fernando Purnomo
JURNAL WIDYA MEDIKA Vol 6, No 2 (2020): October
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v6i2.2789

Abstract

Health Associated Infection (HAI) in neonates can increase neonatal infection risk, which is a fairly frequent cause of neonatal death. Microorganisms that are quite often found to contaminate include gram-negative bacteria such as Escherichia coli and Klebsiella pneumoniae. Gram-negative bacteria are also quite often found to have resistance to antibiotic therapy that is usually given, especially actlactam, and will increase the degree of disease to mortality, this bacterium is called ESBL. The study was conducted to determine the level of ESBL bacterial contamination in health facilities at Dr. Soetomo general hospital. Swabs are taken and biochemical tests were done to identify pathogen species. These isolates were also tested for ESBL production by the double-disc synergy test (DDST). There were 30 samples that contaminate the handle of the baby box, 2 of which (6.67%) tested positive for ESBL. With good hand hygiene, the use of disinfectants in medical devices, floors, walls, and doors, maintenance and replacement of tap water filters, and regular monitoring can reduce the number of bacterial contamination.
The Antibacterial Effect of Ethanol Extract of Garlic (Allium sativum L.) on Methicillin Resistant Staphylococcus aureus (MRSA) In Vitro Utami Meilanie Putri; Maftuchah Rochmanti; Manik Retno Wahyunitisari; Rebekah J. Setiabudi
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14918

Abstract

Objective: To determine the antibacterial activity of ethanol extract of garlic against Methicillin-ResistantStaphylococcus aureus. Method: Dilution test was performed on 9 different concentration of ethanol extractof garlic (1024, 512, 256, 128, 64, 32, 16, 8, 4 mg/mL) dissolved in DMSO 10% and MRSA suspensionon Mueller Hinton broth medium to determine the Minimum Inhibitory Concentration. The MinimumBactericidal Concentration was determined by the result of streaking of MRSA inoculation on the nutrientagar plate. Results: The observation through Mueller Hinton broth medium has shown turbidity both beforeand after incubation because of garlic ethanol extract color which is blackish-brown and thick, while in thenutrient agar medium, MRSA bacteria grew on the media with concentrations 4, 8, 16, 32, 64, & 128 mg/mL and unable to grow at concentrations of 256, 512, and 1024 mg/mL. Conclusion: The MIC of ethanolextract of garlic on MRSA could not be determined, and the MBC in this study was 256 mg/mL.
THE CORRELATION BETWEEN SEX, AGE, NUTRITIONAL STATUS AND DIABETES MELLITUS WITH CLINICAL SYMPTOMS MDR PULMONARY TB AT RSUD DR SOETOMO Ni Putu Anggita Medyantari; Soedarsono Soedarsono; Manik Retno Wahyunitisari
Jurnal Kedokteran Syiah Kuala Vol 19, No 2 (2019): Volume 19 Nomor 2 Agustus 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v19i2.18063

Abstract

Abstract. Multi-drug resistance is a condition where drugs rifampicin and isoniazid is not effective in killing bacteria Mycobacterium tuberculosis. Some of the causes of resistance to OAT are the use of a single drug in the treatment of tuberculosis, the use of drugs is inadequate and the irregular drug administration. In RSUD Dr. Soetomo, 25% of MDR Pulmonary TB patients do not want to be treated because symptoms are mild, no pain and feel the treatment is not comparable with the symptoms. This research is to determine the correlation between sex, age, nutritional status and diabetes mellitus with the clinical description of MDR pulmonary TB patients in RSUD Dr. Soetomo. Hence, it is expected  people can make the earliest possible prevention against this disease. In addition, clinicians are more aware of the symptoms of MDR Pulmonary TB. Method in this research is  descriptive observational study research. There were  103 patients taken as the sample of the study. Research variables consist of sex, age, nutritional status, and diabetes mellitus. The obtained data were analyzed by administering Chi- square analysis. The results of this study showed that each  variable tested in this study (sex, age, nutritional status and diabetes mellitus) associated with the symptoms of MDR Pulmonary TB  has  p 0.05 which means no significant relationship. There are no relation between sex, age, nutrition status and comorbidities with symptom of MDR pulmonary TB.  Keywords: multidrug resistance, sex, age, nutritional status, diabetes mellitus Multi-drug resistance adalah suatu kondisi dimana obat rifampisin dan isoniazid tidak efektif dalam membunuh bakteri Mycobacterium tuberculosis. Beberapa penyebab resistensi terhadap OAT adalah penggunaan obat tunggal dalam pengobatan tuberkulosis, penggunaan obat tidak memadai dan pemberian obat tidak teratur. Di RSUD Dr. Soetomo, 25% pasien TB paru MDR tidak mau diobati karena gejalanya ringan, tidak terasa sakit dan rasakan pengobatannya tidak sebanding dengan gejalanya. Penelitian ini bertujuan untuk mengetahui hubungan antara jenis kelamin, umur, status gizi dan diabetes mellitus dengan gambaran klinis pasien TB paru MDR di RSUD Dr. Soetomo. Sehingga diharapkan masyarakat bisa melakukan pencegahan paling dini terhadap penyakit ini. Selain itu, dokter lebih sadar akan gejala TB paru MDR. Metode yang digunakan dalam penelitian ini adalah penelitian observasional deskriptif. Terdapat 103 pasien yang diambil sebagai sampel penelitian. Variabel penelitian terdiri dari jenis kelamin, umur, status gizi, dan diabetes mellitus. Data yang diperoleh dianalisis dengan analisis Chi-square. Hasil penelitian ini menunjukkan bahwa setiap variabel yang diuji (jenis kelamin, usia, status gizi dan diabetes mellitus) yang dihubungkan dengan gejala TB paru MDR memiliki p 0,05 yang berarti tidak ada hubungan yang signifikan. Kesimpulannya, tidak ada hubungan antara jenis kelamin, usia, status gizi dan komorbiditas dengan gejala TB paru MDR.
EFEKTIVITAS EKSTRAK SELEDRI (Apium graveolens L. var. secalinum Alef.) TERHADAP PERTUMBUHAN BAKTERI Streptococcus mutans SEBAGAI ALTERNATIF OBAT KUMUR Marani Bafianti Suwito; Manik Retno Wahyunitisari; Sri Umijati
Jurnal Kedokteran Syiah Kuala Vol 17, No 3 (2017): Volume 17 Nomor 3 Desember 2017
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v17i3.9150

Abstract

ABSTRAKStreptococcus mutans merupakan bakteri yang paling penting dalam proses terjadinya karies gigi dan juga merupakan bakteri Gram positif yang memiliki kemampuan untuk menghasilkan bau nafas tak sedap. Berbagai tindakan telah dilakukan untuk menjaga kesehatan rongga mulut, salah satunya adalah menggunakan obat kumur. Chlorhexidine gluconate telah menjadi gold standard sejak 1940 karena efektif dan mempunyai spektrum antimikroba yang luas. Meskipun demikian, penggunaan chlorhexidine gluconate dalam jangka panjang tidak dianjurkan karena efek samping yang dapat terjadi. Berdasarkan hal tersebut penulis ingin memberi solusi alternatif dengan memanfaatkan ekstrak seledri (Apium graveolens L. var secalinumAlef) yangmengandung flavonoid, saponin, dan tannin yang merupakan senyawa bersifat antibakteri. Jenis penelitian yang digunakan adalah eksperimental laboratorium dengan metode dilusi untuk menentukan Konsentrasi Hambat Minimal (KHM) dan Konsentrasi Bunuh Minimal (KBM). Penelitian ini menggunakan 6 tabung dan 2 tabung kontrol dengan konsentrasi 100%, 50%, 25%, 12,5%, 6,25%, dan 3,125%. Berdasarkan hasil pengamatan, Konsentrasi Hambat Minimal (KHM) yang didapatkan adalah 3,125%, sedangkan untuk Konsentrasi Bunuh Minimal (KBM) tidak dapat ditentukan. Tidak dapat ditentukan diduga terkait dengan rendahnya senyawa aktif pada sampel penelitian ini. serta diduga terdapat degradasi senyawa aktif dalam ekstrak seledri akibat paparan sinar matahari, panas, dan pH. Berdasarkan hal tersebut, dapat disimpulkan bahwa ekstrak seledri (Apium graveolens L. var secalinum Alef)  mampu menghambat pertumbuhan bakteri Streptococcus mutans namun tidak dapat membunuh bakteri tersebut.Kata Kunci :Ekstrak Seledri (Apium graveolens L. var secalinum Alef) – Streptococcus mutans – metode dilusiStreptococcus mutans is the most important bacteria in the process of dental caries and also Gram positive bacteria  bakteri that has ability to produce bad odor. Various measures have been taken to maintain oral health, one of them is using mouthwash. Chlorhexidine gluconate has became the gold standard since 1940 because it’s effectiveness and has a broad antimicrobial spectrum. However, the long-term use of chlorhexidine gluconate is not recommended because of possible side effects that can occur later on. Based on this, the author wanted to show an alternative solution by utilizing celery extract (Apium graveolens L. var secalinum Alef) containing flavonoids, saponins, and tannins which are antibacterial compounds. This research is designed as an  experimental  laboratory with dilution method  to determine Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC). This study using 6 tubes and 2 control tubes with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, and 3.125%. The Minimal Inhibition Concentration (MIC) is 3.125%, while for Minimum Bactericidal Concentration (MBC) there is no result. This result might be related to the use of crude extract and minimal amount of active compound in this sample. Besides, the amount of active compound can be degraded by exposure of light, heat, and pH.Based on the result, celery extract (Apium graveolens L. var secalinum Alef)   able to inhibit the growth of Streptococcus mutans bacteria but can not kill the bacteria. Keywords :Celery Extract (Apium graveolens L. var secalinum Alef) – Streptococcus mutans – dilution method
Profile of Tinea Corporis and Tinea Cruris in Dermatovenereology Clinic of Tertiery Hospital: A Retrospective Study Selvia Yuliani Dwi Ratih Sanggarwati; Manik Retno Wahyunitisari; Linda Astari; Evy Ervianti
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 33 No. 1 (2021): APRIL
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikk.V33.1.2021.34-39

Abstract

Background: Tinea corporis and cruris are dermatophytes that infect the skin, and they are caused by Trichophyton sp., Microsporum sp., and Epidermophyton sp. Dermatophyte fungal-infect keratin-containing skin classified by the body’s location, such as tinea corporis (skin besides haired area, body, hands, or feet) and tinea cruris (groin & perineal region). Purpose: To evaluate the clinical profiles and treatments of tinea corporis and cruris at Dermatology and Venereology (DV) outpatient clinic Dr. Soetomo General Academic Hospital in 2018. Methods: A retrospective-descriptive study based on medical records of DV outpatient clinic Dr. Soetomo General Academic Hospital, Surabaya. Data collected from August to December 2019. Tinea corporis and cruris profiles evaluation based on age, sex, occupation, precipitating factors, complaints, illness duration, family history, lesion’s location and description, and therapy. Result: A total of 164 patients (35 tinea corporis, 76 tinea cruris, and 53 tinea corporis and cruris), predominantly female, age 46–55 years, came treatment less-than-a-month cases, excessive sweating, itching, tinea corporis lesion’s location on the face and body, tinea cruris in  groin, tinea corporis and cruris on body and groin, erythematous macules, firm borders, active margins, scales, central healing, positive KOH 10–20% examination, miconazole cream therapy 2%, griseofulvin therapy, griseofulvin oral and ketoconazole cream 2% combination therapy. Discussion: Tinea corporis and cruris are common in females as their history and symptoms supported by the characteristics of laboratory examination, the presence of hyphae in potassium hydroxide (KOH) 10–20%. Most patients were prescribed with oral griseofulvin, especially in large lesions cases.
The Characteristics of Children with UTI Due to ESBL-producing Bacteria at Dr. Soetomo General Hospital, Surabaya Ida Fitriawati; Manik Retno Wahyunitisari; Risky Vitria Prasetyo; Dwiyanti Puspitasari; Tuksin Jearanaiwitayakul
Biomolecular and Health Science Journal Vol. 4 No. 1 (2021): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v4i1.25392

Abstract

Introduction: Urinary tract infection (UTI) by extended-spectrum beta-lactamase-producing bacteria often results in a delay in obtaining appropriate antibiotics. The information on patients’ clinical characteristics is necessary for early recognition and the selection of empiric antibiotic therapy. This study aims to investigate the clinical characteristics and the length of therapy of patients with urinary tract infections by those resistant bacteria.Method: This study utilizes a cross-sectional design. Medical records of hospitalized children aged 1-18 months with UTI due to ESBL-producing bacteria at Dr. Soetomo general hospital between January 1, 2017 - July 20, 2020, were reviewed retrospectively. Variables of interest were the demographic data, underlying diseases, causative organism, clinical presentation, maximal body temperature, and length of antibiotic therapy.Results: Among 37 patients enrolled, 25 patients were female. The incidence of urinary tract infection in children was dominated by age 1-12 months old (37.8%). Urological abnormalities were presented in 62.2% of patients. ESBL-producing Escherichia coli was the most common isolated uropathogen (62.2%). High fever was found in 10/28 patients (35.7%). In 17 patients (45.9%), the total duration of antibiotic therapy was 8-14 days.Conclusion: In children with UTI, especially in the infant group, who had urological abnormality or present with a high fever, and who do not respond to empiric therapy should be suspected of developing UTI due to ESBL-producing bacteria.
The Antibacterial Activity of Amber Honey and White Honey on Pseudomonas aeruginosa Multi Resistant (PaMR) and Methicillin Resistant Staphylococcus aureus (MRSA) Faisal Yusuf Ashari; Setio Harsono; Manik Retno Wahyunitisari
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 11 No. 2 (2020): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V11I22020.74-78

Abstract

Introduction: Infection of burn wound is a chronical disturbance to the skin, which is caused by Staphylococcus aureus and Pseudomonas aeruginosa, but lately many cases found that both bacteria have been resistant to the antibiotic. To overcome the infection caused by those bacteria, it is important to search for natural substance that has antibacterial activity to both bacteria. The power of natural substance is underestimated. Studies reveal that honey can be more effective for healing burns than modern medicine. The previous research revealed that amber honey and white honey were used as an alternative treatment against Pseudomonas aeruginosa Multi Resistant (PaMR) and Methicillin Resistant Staphylococcus aureus (MRSA). The aim of this study was to evaluate the antibacterial activity of amber honey and white honey on PaMR and MRSA.Methods: This research was conducted by performing antibacterial test phase, determining Minimum Inhibitory Concentration (MIC), and determining comparison value of antibacterial activity from amber honey and white honey to tetracycline.Results: The result of this research showed that both amber honey and white honey have antibacterial activity to both bacteria, by way of stronger antibacterial activity from white honey than amber honey. Amber honey and white honey have the same PaMR activity. MIC of white honey was 7.1% (to MRSA) and 12.3% (to PaMR), while MIC of amber honey was 10% (to MRSA) and 12.5% (to PaMR). The comparison value of antibacterial activity between amber honey and white honey to tetracycline for MRSA was equal to 1:1.08 x 10-4 and 1:1.62 x 10-4. The comparison value of antibacterial activity between amber honey and white honey to tetracycline for PaMR was equal to 1:5.62 x 10-6 and 1:1.03 x 10-5 .Conclusion: Amber honey and white honey have antibacterial activity against MRSA and PaMR. 
PENINGKATAN PENGETAHUAN DAN KEAHLIAN DIAGNOSIS MIKROSKOPIS BTA PETUGAS LABORATORIUM DI SORONG PADA ERA PANDEMI COVID-19 Rebekah J Setiabudi; Ni Made Mertaniasih; Manik Retno Wahyunitisari
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 5 No. 1 (2021): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v5i1.2021.111-115

Abstract

ABSTRAKPenyakit Tuberkulosis (TB) Paru merupakan salah satu penyebab morbiditas dan mortalitas penting di dunia. Indonesia masih merupakan salah satu negara yang mempunyai beban TB yang terbesar diantara 5 negara yaitu: India, China, Nigeria dan Pakistan. Kota dan Kabupaten Sorong merupakan salah satu daerah di Propinsi Papua Barat dimana TB masih menjadi salah satu permasalahan kesehatan. Pemeriksaan mikroskopis TB dengan metode Ziehl Neelsen, menurut WHO, masih merupakan sarana diagnostik yang sensitif, spesifik, praktis, dan juga murah. Belum terstandardisasinya kinerja petugas laboratorium TB juga menjadi kendala tersendiri dalam diagnosis TB dengan metode Ziehl Neelsen. Munculnya pandemi Covid-19 yang melanda dunia termasuk Indonesia, membuat berbagai kegiatan menjadi tertunda. Kegiatan Pengabdian Kepada Masyarakat di tengah pandemi Covid-19 ini terpaksa dilakukan dalam bentuk “workshop online”. Dalam meningkatkan pengetahuan digunakan aplikasi zoom untuk kuliah penyegaran, memberikan informasi terbaru terkait TB di Indonesia dan pengetahuan tambahan lainnya. Sedangkan dalam meningkatkan keahlian dilakukan praktek pembuatan sediaan mikroskopis TB dan latihan pembacaan sediaan mikroskopis TB yang dilaksanakan secara offline di laboratorium Mikrobiologi Rumah Sakit JP Wanane, yang dimonitor dan dibimbing oleh Dokter Spesialis Mikrobiologi Klinik yang bertugas di sana. Melalui kegiatan Pengabdian Kepada Masyarakat yang dilakukan oleh Universitas Airlangga ini, diharapkan dapat membangkitkan lagi semangat, melakukan penyegaran serta menambah pengetahuan dan ketrampilan bagi tenaga kesehatan di daerah untuk dapat melakukan tugasnya dengan baik sehingga Program Pengendalian Infeksi Tuberkulosis dapat kembali berjalan sebagaimana seharusnya. Kata kunci : tuberkulosis, pemeriksaan mikroskopis, sorong, pandemi Covid-19 ABSTRACTPulmonary Tuberculosis (TB) is one of the most important causes of morbidity and mortality in the world. Indonesia is still one of the countries with the largest burden of TB among 5 countries, namely: India, China, Nigeria and Pakistan. Sorong City is one of the areas in Western Papua, Indonesia, where TB is still a health problem. According to WHO, microscopic examination of TB by the Ziehl Neelsen method is still a sensitive, specific, practical, and inexpensive diagnostic tool. The unstandardized performance of TB laboratory personnel has also become an obstacle in itself in diagnosing TB with the Ziehl Neelsen method. The emergence of the Covid-19 pandemic has delayed various activities. Community Service Activities in the midst of the Covid-19 pandemic had to be carried out in the form of an "online workshop". In increasing knowledge, the zoom application is used for refresher lectures, providing the latest information related to TB in Indonesia and other additional knowledge. Meanwhile, in increasing expertise, the practice of making TB microscopic preparations and reading exercises for TB microscopic preparations was carried out offline in the Microbiology laboratory of the JP Wanane Hospital, which was monitored and guided by the Clinical Microbiology Specialist who served there. Through this Community Service activity carried out by Airlangga University, it is hoped that it can revive enthusiasm, refresh and increase knowledge and skills for health workers in the regions to be able to carry out their duties properly so that the Tuberculosis Infection Control Program can resume running as it should.Keyword : tuberculosis, microscopic examination, Sorong, Covid-19 pandemic
Co-Authors Agung Dwi Wahyu Widodo Alimsardjono, Lindawati Anindita Rehana BaiqIsti Hijriani Deby Kusumaningrum Dwiyanti Puspitasari, Dwiyanti Eko Budi Koendhori, Eko Budi Erwin Astha Triyono Evy Ervianti Faisal Yusuf Ashari Feriawan Tan Firnanda Iptita Dewi Hasan, Helmia Ida Fitriawati Ivan Sofian Wibowo Linda Astari Lynda Hariani Maftuchah Rochmanti Marani Bafianti Suwito Marsha Zahrani Martono Tri Utomo Melisa Indah Mustikasari Ni Made Mertaniasih Ni Putu Anggita Medyantari Nicholas Fernando Purnomo Pepy Dwi Endraswari, Pepy Dwi Rebekah Setiabudi, Rebekah Risky Vitria Prasetyo S. Soedarsono Selvia Yuliani Dwi Ratih Sanggarwati Setio Harsono Silvia Sutandhio Sri Sumijati, Sri Sulistiawati Sulistiawati Titiek Sulistyowati Tuksin Jearanaiwitayakul Umiastuti, Pirlina Utami Meilanie Putri Wiwin Retnowati