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Effectiveness of clove oil as anesthesia of Sergeant Major Abudefduf vaigiensis (Quoy & Gaimard, 1825) Amris, Ainun Ayu Utami; Rahim, Sri Wahyuni; Yaqin, Khusnul
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 4, No 1 (2020)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.4.1.21-28

Abstract

The increasing demand for live ornamental fish for export needs triggers fishermen to start looking for alternative fishing techniques that are more effective and efficient. The technique commonly used is the anesthetic technique using potassium cyanide. The use of cyanide has been banned by the government because of the many impacts it has caused such as the degradation of coral reef ecosystems, high non-target fish mortality and pollution of coastal areas. Clove oil is an alternative anesthetic which can be utilized with many advantages compared to cyanide. The sampled fish used was sergeant major (Abudefduf vaigiensis) as many as 15 fish with a size of 9-12 cm obtained from fishermen's catch using a scoop. The purpose of this study was to determine the optimal concentration of clove oil as an anesthetic of Sergeant Major Fish (Abudefduf vaigiensis) based on induction time  and recovery time. The experimental design used was a Completely Randomized Design (CRD). Analysis of the data used is One-Way ANOVA parametric analysis and Kruskal Wallis nonparametric analysis. The results obtained are five series of concentrations (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) which have a significant effect on induction time (20 ppm and 60 ppm) of sergeant major fish (Abudefduf vaigiensis) (P<0.05) The treatment of five concentration series (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) did not have a significant effect on the recovery time of the Sergeant Major Fish (Abudefduf vaigiensis) (P>0.05).
KONSENTRASI MIKROPLASTIK PADA KERANG MANILA Venerupis philippinarum DI PERAIRAN MACCINI BAJI, KECAMATAN LABAKKANG, KABUPATEN PANGKAJEN KEPULAUAN, SULAWESI SELATAN Wahdani, Amelia; Yaqin, Khusnul; Rukminasari, Nita; ., Suwarni; ., Nadiarti; Inaku, Dwi Fajriyati; Fachruddin, Liestiaty
Maspari Journal : Marine Science Research Vol 12, No 2 (2020)
Publisher : UNIVERSITAS SRIWIJAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/maspari.v12i2.12809

Abstract

Mikroplastik di perairan menjadi permasalahan yang cukup serius bagi organisme perairan. Organisme filter feeder seperti kerang memiliki resiko yang cukup besar untuk mengakumulasi mikroplastik ke dalam tubuhnya. Salah satu jenis organisme tersebut ialah kerang manila (Venerupis philippinarum) yang banyak terdapat di Perairan Maccini Baji, Kecamatan Labakkang, Kabupaten Pangkajene Kepulauan, Sulawesi Selatan. Penelitian ini bertujuan untuk mengetahui keberadaan dan konsentrasi mikroplastik yang terdapat pada daging kerang manila (Venerupis philippinarum). Pengambilan sampel kerang dilakukan dengan metode sampling acak berlapis (stratified random sampling), sehingga diperoleh sampel sebanyak 118 ekor. 118 sampel kerang manila tersebut kemudian dibagi menjadi tiga kelompok ukuran panjang cangkang kerang yaitu kelas A (3,11 – 3,86 cm), kelas B (3,87 – 4,82 cm), kelas C (4,83 – 6,01 cm). Pengamatan partikel mikroplastik dilakukan dengan menggunakan mikroskop stereo. Hasil pengamatan menunjukkan sebanyak 61 sampel (51,69%) kerang manila mengandung partikel mikroplastik. Mikroplastik yang ditemukan berbentuk fiber dan fragmen, dengan warna dominan biru, hitam, dan transparan. Ukuran mikroplastik yang ditemukan berkisar antara 0,090 – 4,919 mm. Nilai rata-rata konsentrasi mikroplastik pada masing-masing kelompok ukuran panjang cangkang kerang secara berurutan yaitu 0,6129 item/g, 0,6303 item/g, dan 0,2198 item/g. Kata kunci: Fiber, kerang manila, konsentrasi mikroplastik, Maccini Baji, Venerupis philippinarum.
Ecotoxicological Assessment of Aquatic Genotoxicity Using the Comet Assay KHUSNUL YAQIN
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (46.994 KB) | DOI: 10.4308/hjb.13.3.124

Abstract

Comet assay is a novel biological analysis, which is a sensitive, flexible, simple, rapid, and inexpensive method to assess aquatic genotoxicant. Since Singh and co-workers developed the method in 1988, its use has increased exponentially in various fields. This review discourses on the application of this assay in aquatic ecosystems. Various types of cells from various aquatic organisms have been tested by various genotoxicant both direct- and indirect-acting using the comet assay. The applications of this assay suggest that it is a useful assay to assess aquatic genotoxicants. However, there are some factors, which should be taken into account when using this assay as aquatic ecotoxicological assessment device such as inter-animal and cell variability. Key words: comet assay, aquatic genotoxicant, aquatic organism, ecotoxicological assesment
The Use of Selected Biomarkers, Phagocytic and Cholinesterase Activity to Detect the Effects of Dimethoate on Marine Mussel (Mytilus edulis) KHUSNUL YAQIN; BIBIANA WIDIATI LAY; ETTY RIANI
HAYATI Journal of Biosciences Vol. 15 No. 1 (2008): March 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.987 KB) | DOI: 10.4308/hjb.15.1.32

Abstract

Effects of organophosphorous pesticide, dimethoate on blue mussels, Mytilus edulis using selected biomarkers have been studied. Mussels were exposed to serial dilutions of dimethoate, 7.88, 15.75, 31.35, and 63.00 µg/l including positive and negative controls for 14 days. The suppression effects of dimethoate on phagocytic activity significantly occurred at two lowest concentrations of dimethoate (7.88 and 15.75 µg/l), but stimulation effects significantly emerged at the following highest concentrations (31.35 and 63.00 µg/l). The declining tendency of the cholinesterase (ChE) activity (23% lower than the control) appeared when mussels exposed to 7.88 and 15.75 µg/l dimethoate. Moreover, the significant inhibition of the ChE activity occurred at 31.35 µg/l dimethoate exposure. This study suggested that the phagocytic and the ChE activity are useful biomarkers for assessing the affects of organophosporous pesticide, dimethoate on neuro-immune system of blue mussels, M. edulis. Key words: dimethoate, cholinesterase, phagocytic, blue mussels
Effectiveness of clove oil as anesthesia of Sergeant Major Abudefduf vaigiensis (Quoy & Gaimard, 1825) Ainun Ayu Utami Amris; Sri Wahyuni Rahim; Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 4, No 1 (2020)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (936.264 KB) | DOI: 10.29239/j.akuatikisle.4.1.21-28

Abstract

The increasing demand for live ornamental fish for export needs triggers fishermen to start looking for alternative fishing techniques that are more effective and efficient. The technique commonly used is the anesthetic technique using potassium cyanide. The use of cyanide has been banned by the government because of the many impacts it has caused such as the degradation of coral reef ecosystems, high non-target fish mortality and pollution of coastal areas. Clove oil is an alternative anesthetic which can be utilized with many advantages compared to cyanide. The sampled fish used was sergeant major (Abudefduf vaigiensis) as many as 15 fish with a size of 9-12 cm obtained from fishermen's catch using a scoop. The purpose of this study was to determine the optimal concentration of clove oil as an anesthetic of Sergeant Major Fish (Abudefduf vaigiensis) based on induction time  and recovery time. The experimental design used was a Completely Randomized Design (CRD). Analysis of the data used is One-Way ANOVA parametric analysis and Kruskal Wallis nonparametric analysis. The results obtained are five series of concentrations (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) which have a significant effect on induction time (20 ppm and 60 ppm) of sergeant major fish (Abudefduf vaigiensis) (P<0.05) The treatment of five concentration series (20 ppm, 30 ppm, 40 ppm, 50 ppm, 60 ppm) did not have a significant effect on the recovery time of the Sergeant Major Fish (Abudefduf vaigiensis) (P>0.05).
Nutrient Absorption Rate of Oryzias celebensis Embryo Yulia Indah Sari Lalombo; Khusnul Yaqin; Sharifuddin bin Andy Omar
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 2 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.2.67-71

Abstract

Oryzias celebensis is a species of medaka fish in South Sulawesi. During incubation, the growth and development of these fish embryos depend on the nutrients stored in the eggs. Therefore, this study aimed to determine the volume size of egg yolks and oil droplets and the absorption rate of egg yolks of O. celebensis embryos that reared in different incubation media. The study was conducted using a completely randomized design (CRD) with four rearing media treatments, namely A (Early Rearing Media), B (bottled water), C (Pattunuang river water) and D (well water) with five replications . The results showed that the volume of egg yolk and O. celebensis embryo oil in each medium showed a decrease in volume size along with the embryonic development stage. The absorption rate of yolk in O. celebensis embryos in media A had the biggest yolk absorption with an average 0.015±0.038 mm3 while media D had the smallest yolk absorption with an average 0.011±0.022 mm3.
Microplastic: pollution issue and seafood security Khusnul Yaqin
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 1 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.1.25-27

Abstract

At the first time, plastic materials were produced to facilitate various activities of human life. Plastic materials that are flexible and durable have been used by humans to meet various needs to support their daily activities. Starting from human activities from waking up to going back to sleep, nowadays it cannot be separated from the use of plastic materials. This then makes humans "addicted" to plastic materials. It is as if human life cannot be separated from the use of plastic materials. Various research results in the field of pollution both on land and the sea, plastic materials that are not managed properly can contaminate human life, either directly or indirectly, to food sources, especially food from the sea.
Microplastics contamination in green mussels Perna viridis in Pangkajene Kepulauan Waters, South Sulawesi, Indonesia Ramli Ramli; Khusnul Yaqin; Nita Rukminasari
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 5, No 1 (2021)
Publisher : Sekolah Tinggi Ilmu Pertanian Wuna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.5.1.1-5

Abstract

Microplastics is a problem that has been concerning, especially in marine habitat. The presence of microplastics in large quantities will have an impact on the environment and marine organisms. Marine organisms that are vulnerable to contamination by microplastics pollutants namely green mussels (Perna viridis). This study aims to determine the microplastics forms and concentration found in green mussels (Perna viridis) in Maccini Baji Waters, Labakkang District, Pangkejene Kepulauan Regency, South Sulawesi. The sampling of the mussels was carried out by the method of purposive random sampling with the sample was grouped into 3 groups of shells lengths, namely 2-3.9 cm, 4-5.9 cm and 6-7.9 cm with 33 individuals for each group. Microplastics observations were carried out using a stereo microscope. In addition to analyze microplastics concentrations, an analysis of the frequency of microplastics presence in shells was also carried out. The results showed that the microplastics was found in the form of fiber and fragments with several colors namely clear white, blue, black, red, and purple. The frequency of microplastics presence in green mussels was above 50% with an average of 71.7% exposure to microplastics. The highest frequency of microplastics presence and concentration in the range of shell lengths accounting for 2-3.9 cm.
PENGGUNAAN INDEKS KONDISI KERANG HIJAU (Perna viridis) SEBAGAI BIOMARKER UNTUK MENDETEKSI PENGARUH PENGASAMAN LAUT TERHADAP TOKSISITAS LOGAM PB Khusnul Yaqin; Arnold Kabangnga
Torani Journal of Fisheries and Marine Science Vol. 25 No. 1 (2015)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.149 KB) | DOI: 10.35911/torani.v25i1.259

Abstract

Penelitian ini bertujuan untuk menganalisis kemampuan indeks kondisi kerang hijau (Perna viridis) dalammendeteksi pengaruh pengasaman laut terhadap toksisitas logam Pb. Penelitian dilaksanakan di LaboratoriumPenangkaran dan Rehabilitasi Ekosistem Laut Fakultas Ilmu Kelautan dan Perikanan, Universitas Hasanuddin. Rancangan yang digunakan dalam penelitian ini adalah rancangan faktorial. Kerang hijau dengan ukuranpanjang 5-6 cm diberi perlakuan dengan paparan konsentrasi logam Pb 0 mg/l (kontrol), 0,008 mg/l, 0,08 mg/ldan 0,8 mg/l, pada kondisi pH (level asidifikasi) air media hidup yaitu 6,2, 7,7, 8,2. Pemaparan dilakukanselama 96 jam. Pengukuran indeks kondisi dilakukan dengan mengukur panjang, tinggi, lebar, berat dagingkering, dan volume ruang internal cangkang. Nilai indeks kondisi dianalisis dengan analisis varian desainfaktorial. Hasil analisis menunjukkan terdapat pengaruh signifikan konsentrasi logam Pb terhadap penurunannilai indeks kondisi kerang (p<0.05). Pengaruh logam Pb terjadi pada CI 1, CI 3 dan CI 4. Meskipun perlakuan pH tidak menunjukkan pengaruhnya terhadap indeks kondisi, tetapi secara statistik diketahui bahwa terdapat interaksi antara pH dan Pb dalam memengaruhi indeks kondisi C1 dan C3 (p<0,05). Hal ini menunjukkanperlakuan pH memunyai peran dalam toksisitas logam Pb terhadap penurunan nilai indeks kondisi kerang hijau.Kata kunci :Indeks kondisi, Biomarker, Logam Pb, Perna viridis, Penagasaman laut
The Concentration of Lead in Windowpane Oyster (Placuna placenta) and Potential of Condition Index as a Morphological Biomarker to Detect Metal Pollution Khusnul Yaqin; Liestiaty Fachruddin
Torani Journal of Fisheries and Marine Science VOLUME 1 NOMOR 2, JUNI 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v1i2.4437

Abstract

As a filter feeder, windowpane oyster, Placuna placenta, which lives as a sedentary animal is very appropriate to be used as an eco-sentinel organism in marine bio-monitoring campaign.  The study of lead (Pb) content in the tissue and shell of P. placenta was conducted in coastal waters of Mandalle, Pangkajene Kepulauan District, South Sulawesi.  A hundred of oysters were collected from coastal waters of Mandalle.  Morphometry parameters which were length, high, width, and dry weight of tissue were measured in the laboratory.  Those parameters were used for determination of Condition Index (CI).  Metal was analyzed by Atomic Absorption Spectrometry.  The results showed that the contents of Pb in tissue and shell were 0.021 and 0.014 mg/kg DW (dry weight) respectively.  The concentration of Pb in tissue was not overreach by BPOM 2009 Number Hk.00.06.1.52.4011 of 1.5 mg / kg DW (dry weight).  After calculating Maximum Tolerable Intake (MTI), we found that MTI was 7.79 kg/day.  In terms of marine monitoring campaign, we can theoretically use the CI as a morphological biomarker.  After calculating the relationship between Pb in tissue and shell, we found that the value of CI was not correlated significantly with Pb content in tissue and shell.  However, the correlation coefficient between Pb in tissue and CI was stronger than that in the shell.  The potential of CI of windowpane oyster as a morphological biomarker was discussed.Keywords: Placuna placenta, lead, MTI, morphological biomarker