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All Journal Jurnal e-Biomedik JURNAL PESISIR DAN LAUT TROPIS Jurnal MIPA PHARMACON Jurnal Bios Logos JURNAL KEDOKTERAN KOMUNITAS DAN TROPIK Journal of Tropical Ethnobiology Vivabio : Jurnal Pengabdian Multidisiplin ZOOTEC Jurnal Ilmiah Sains
Edwin de Queljoe
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Veterinary

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UJI EFEK ANALGETIK EKSTRAK ETANOL DAUN KUMIS KUCING (Ortosiphon aristatus (Blume) Miq.) TERHADAP TIKUS PUTIH JANTAN Nurfitri, Mayang Marsina; Queljoe, Edwin de; Datu, Olvie S.
PHARMACON Vol 10, No 4 (2021): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.10.2021.37413

Abstract

ABSTRACTKumis kucing leaves (Ortosiphon aristatus (Blume) Miq) contain flavonoid compounds, namely sinensetin, eupatorine, 3'-hydroxy-5,6,7,4'-tetramethoxyflavone and rosmarinic acid which have analgetic effects. This study aims to determine the analgetic effect of the ethanol extract of kumis kucing leaves at different doses, such as at doses of 5 mg/200gBW rats, 10 mg/200gBW rats and 20 mg/200gBW rats. This study employed a laboratory experimental research design using Rattus norvegicus. Analgetic effect was tested using the heat stimulation method which utilized a hot plate with a temperature of 55℃. The response observed was in the form of licking the hind legs or jumping from before giving the test material to after giving the test material for 30, 60, 90 and 120 minutes, respectively. The results demonstrated that the ethanol extract of the kumis kucing leaves had better analgetic activity than the negative control at doses of 5 mg/200gBW rats, 10 mg/200gBW rats, and 20 mg/200gBW rats. According to the results of statistical data analysis with One Way Anova and followed by the LSD test with a 95% confidence level, it showed that the ethanol extract of the cat's whisker leaves had an analgetic effect. Where the best dose among the three doses was doses of 4.125 mg/165gBW rats. Keywords: Analgetic, Kumis Kucing Leaves (Ortosiphon aristatus (Blume) Miq), Rattus norvegicus  ABSTRAKDaun kumis kucing (Ortosiphon aristatus (Blume) Miq.) mengandung senyawa flavonoid yaitu sinensetin, eupatorine, 3’-hydroxy-5,6,7,4’-tetramethoxyflavone dan asam rosmarinik yang memiliki efek analgetik. Penelitian ini bertujuan untuk mengetahui adanya efek analgetik pada ekstrak etanol daun kumis kucing dengan dosis berbeda-beda yaitu dengan dosis 5 mg/200gBB tikus, 10 mg/200gBB tikus dan 20 mg/200gBB tikus. Penelitian ini menggunakan rancangan penelitian eksperimental laboratorium dengan menggunakan hewan uji tikus putih jantan. Pengujian efek analgetik dilakukan dengan metode rangsangan panas menggunakan hot plate dengan suhu 55℃. Respon yang diamati berupa gerakan menjilat kaki belakang atau melompat dari sebelum pemberian bahan uji hingga sesudah pemberian bahan uji dengan waktu berturut-turut 30, 60, 90 dan 120 menit. Hasil penelitian menunjukan bahwa ekstrak etanol daun kumis kucing dengan dosis 5 mg/200gBB tikus, 10 mg/200gBB tikus dan 20 mg/200gBB tikus menunjukkan aktivitas analgetik lebih baik dibandingkan kontrol negatif. Dari hasil analisis data secara statistika dengan One Way Anova dan dilanjutkan dengan uji LSD dengan taraf kepercayaan 95% menunjukkan bahwa ekstrak etanol daun kumis kucing memiliki efek analgetik. Dimana dosis terbaik diantara ketiga dosis tersebut ialah dosis 5 mg/200gBB tikus. Kata kunci: Analgetik, Daun Kumis Kucing (Ortosiphon aristatus (Blume) Miq), Rattus norvegicus)
AMPLIFIKASI GEN COI DARI SAMPEL DARAH ULAR DENGAN MENGGUNAKAN BEBERAPA PASANGAN PRIMER UNIVERSAL Pahlevi, Dylan R.; de Queljoe, Edwin; Kolondam, Beivy J.
ZOOTEC Vol 39, No 2 (2019)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.387 KB) | DOI: 10.35792/zot.39.2.2019.25413

Abstract

AMPLIFICATION OF COI GENE FROM SNAKE BLOOD SAMPLES USING TWO UNIVERSALPRIMER PAIRS. This study aims to amplify COI (Cytochrome Oxidase Subunit I) gene fragments from snake blood. Four samples were obtained from four different snake individuals that were captured in Tapahan Telu Waterfall, Kali Village, Minahasa Regency. Total DNA from the sample was isolated and then the COI gene was amplified through the PCR (Polymerase Chain Reaction) reaction using two primer pairs, LCO1490-HCO2198 and FF2d-FR1d. These four samples were successfully amplified using different primers, i.e. DRP1 and DRP3 by FF2d-FR1d and DRP2 and DRP4 by LCO1490-HCO2198 primers. The success of amplification marked by the presence of 710 bp (LCO1490-HCO2198) and 707 bp (FF2d-FR1d) bands, which were indicated by those bands were located close to the standard 750 bp DNA ladder. Key words: Snake, Amplification, COI gene, Primers, PCR
The Diversity of Medicinal Herbs of Bogani Ethnic in Bolaang Mongondow, North Sulawesi Herny E. I. Simbala; Sartje J. Rondonuwu; Edwin De Queljoe
Journal of Tropical Ethnobiology Vol. 2 No. 1 (2005): January 2005
Publisher : The Ethnobiological Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3106.55 KB)

Abstract

As so far, modern medical system has rapidly progress; however traditional medical system is still prevailing for the most Indonesian people. Bogani ethnic in Residency of Bolaang Mongondow still use traditional medical system. As evidence, traditional medicine ingredients are still used in their daily living. In the case, traditional medicine was used to treat many diseases, from minor diseases (headache, cough, and influenza, to the acute ones such as lung inflamed (113C), liver and tetanus. The research objectives are : (1) to inventory and describe the medicine plant species; (2) to study plant usage as traditional medicine ingredients for many diseases ; (3) as conservation effort of traditional medicine knowledge because most of them not inherited and have limited data, and (4) to collect scientific information regarding the peculiar property of medicine herb where the research information is potential for pharmacology research in order to discover a new medicine ingredient. A rapprochement method for the research is ethno-directed sampling. Following Friedberg (1993) in Purwanto (2002), eihno-directed sampling methods is a data collection of medicine herb material that based on the local knowledge (ethnic) about medicine herbs, treatment technique, ingredients technique and other aspects that related to public health and conducted with ethnoscience approach. Ethnoscience is an approach that enable us to achieve deep understanding and reveal community knowledge system about medicine herb, treatment technique, ingredient technique and other aspect that related to public health. Qualitative and quantitative approach was used as data collection technique. The result showed that there were 56 species of herbs used as traditional medicine material for Bogani ethnics in Bolaang Mongondow; North Sulawesi. The herbs were generally collected from forest area, garden near with settlement area and indeed from cultivation product at yard. There were 34 families of medicine herbs and most of them including family of Euphorbiaceae, Labiatae, Verbenaceae, Araceae, and Asteraceae. Chemical analysis results showed that 8.9 % of medicine herbs contained terpenoid, 42.8 % of medicine herbs contained steroid, 53.5 % contained tannin, 50 % contained flavonoid, 75 % contained alkaloid, and 30 % contained saponin. The analysis indicated that most of herbs positively contained alkaloid as active medicine compound. However the analysis was only as pretest in determining active compound in a medicine herb. There are some medicine herbs classified as endemic should be given priority to be cultivated immediately because their existence are more and more scarce such as Areca vestrana, Musa sp,and Ficus nunahassae. The herbs are almost extinct and need other proper alternative to conserve them.
IDENTIFICATION OF POTENTIAL DIESEL OIL-DEGRADING BACTERIA ISOLATED FROM MANADO SEA PORT BASED ON 16S rRNA GENE Olivia H Abram; Trina E Tallei; Edwin de Queljoe; Beivy J Kolondam
JURNAL ILMIAH SAINS Volume 14 Nomor 2, Oktober 2014
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (289.928 KB) | DOI: 10.35799/jis.14.2.2014.5932

Abstract

ABSTRACT  Petroleum contamination and its derivate in ecosystem are considered as environmental threat all over the world. Some microorganisms exhibit potential to degrade hydrocarbon in contaminated environments. This study aims at identifying potential diesel oil-degrading bacteria grown on artificial media. Bacteria isolated from Manado Sea port were grown in nutrient agar containing artificial diesel oil plus salt water and diesel oil only, respectively. The growing bacteria were isolated and each of them was grown separately to obtain pure isolate. Three bacterial isolates namely AO2, OA3 and OA4 were identified using 16S rRNA gene as Pseudomonas aeroginosa, Klebsiella oxytoca, and Citrobacter sp, respectively. Keywords: diesel oil, diesel oil-degrading bacteria, Manado Sea Port, 16S rRNA gene IDENTIFIKASI BAKTERI YANG BERPOTENSI SEBAGAI PENDEGRADASI MINYAK DIESEL DI ISOLASI DARI PELABUHAN LAUT MANADO   ABSTRAK   Kontaminasi minyak bumi dan turunannya dalam ekosistem dianggap sebagai ancaman lingkungan di seluruh dunia. Beberapa mikroorganisme menunjukkan potensi yang dapat menurunkan hidrokarbon dalam lingkungan yang terkontaminasi. Penelitian ini bertujuan untuk mengidentifikasi bakteri yang berpotensi sebagai pendegradasi minyak yang tumbuh pada media buatan. Bakteri diisolasi dari pelabuhan laut Manado dan ditumbuhkan dalam media NA yang mengandung minyak diesel dengan penambahan air garam buatan dan minyak diesel tanpa air garam buatan. Bakteri yang tumbuh diisolasi dan masing-masing ditanam secara terpisah untuk mendapatkan isolat murni. Tiga isolat bakteri yaitu AO2, AO3 dan AO4 yang telah diidentifikasi menggunakan 16S rRNA gen secara berturut-turut adalah  Pseudomonas aeroginosa, Klebsiella oxytoca, dan Citrobacter sp. Kata kunci: minyak diesel, bakteri pendegradasi minyak diesel, Pelabuhan Laut Manado, gen 16S rRNA
IDENTIFIKASI BAKTERI PADA PEGANGAN ESKALATOR DI SALAH SATU PUSAT PERBELANJAAN DI KOTA MANADO Michelle V. Holderman; Edwin de Queljoe; Sendy B. Rondonuwu
JURNAL ILMIAH SAINS Volume 17 Nomor 1, April 2017
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.07 KB) | DOI: 10.35799/jis.17.1.2017.14901

Abstract

ABSTRAK Pusat perbelanjaan banyak diminati masyarakat karena mempunyai daya tarik internal dan eksternal. Eskalator pada pusat perbelanjaan dilengkapi dengan pegangan tangan, dengan demikian eskalator dapat menjadi media untuk penjangkitan penyakit yang dapat dengan cepat menyebar karena bakteri pada tangan dapat berpindah ke tangan orang lain apabila memegang pegangan dari eskalator tersebut. Tujuan penelitian ini yaitu untuk mengidentifikasi jenis-jenis bakteri yang terdapat pada pegangan escalator. Metode yang digunakan dalam penelitian ini yaitu identifikasi bakteri meliputi pengamatan morfologi dan pewarnaan gram. Berdasarkan hasil penelitian jenis bakteri yang ditemukan yaitu Staphylococcus epidermidis, Stomatococcus sp, Bacillus subtilis. Kata-kata kunci: Pusat perbelanjaan, eskalator, bakteri IDENTIFICATION OF BACTERIA IN HANDRAIL ESCALATOR ON SHOPPING CENTER IN MANADO ABSTRACT Shopping center attracted many people because they have internal and external appeal. Escalators in shopping center equipped with handrails, thus escalator can be a medium for the outbreak of disease can quickly spread because bacteria on the hands can be transferred into other hands when holding the handle of the escalator that. The purpose of this study is to identify the types of bacteria present on escalator handrail. The method used in this research is the identification of bacteria includes observation of morphology and gram stain. Based on the results of the study found that the type of bacteria Staphylococcus epidermidis, Stomatococcus sp, Bacillus subtilis. Kata-kata kunci: Shopping center, escalator, bacteria
Uji aktivitas antihiperurisemia ekstrak etanol daun salam (Sygium Polyanthum) pada tikus putih (Rattus Norvegicus) Meivita Alfriani Sahensolar; Edwin De Queljoe; Surya Sumantri Abdullah
PHARMACON Vol. 12 No. 1 (2023): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

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Abstract

MEIVITA ALFRIANI SAHENSOLAR . Uji aktivitas antihiperusrisemia ekstrak etanol daun salam (Syzgium Polyanthum) pada tikus putih (Rattus Norvegicus)dibawah bimbingan EDWIN DE QUELJOE sebagai ketua dan SURYA SUMANTRI sebagai anggota.Daun salam memiliki kandungan kimia seperti minyak atsiri yang mengandung sitral, eugenol, tannin, dan flavonoida. Penelitian ini bertujuan untuk mengetahui adanya efek antihiperurisemia ekstrak etanol daun salam dengan 3 tingkatan dosis yaitu, 200mg, 400mg, dan 800mg. Perbandingan daun Salam sebanyak 5 kelompok yang terdiri dari 3 ekor tikus disetiap kelompok perlakuan, yaitu CMC 1% (kontrol negatif), Allopurinol 100mg (kontrol positif), ekstrak etanol daun salam dalam dosis 200mg, 400mg, dan 800 mg,yang diinduksi kalium oksonat. Pengamatan dilakukan selama 6 jam. Data diuji dengan menggunakan ANOVA (Analisis Of Variant) ,uji HOMOGENITAS (Test of Homogenity of Variance) dan dilanjutkan dengan uji LSD (Least Significant Different). Hasil uji statistik menunjukan bahwa Dari ketiga dosis, penurunan kadar asam urat terbaik terdapat pada dosis 400 mg. Kata Kunci: Ekstrak Daun Salam (Syzgium Polyanthum) , Antihiperusemia
Uji Efektivitas Antiinflamasi Salep Ekstrak Batang Pohon Pisang Ambon (Musa paradisiaca var. Sapientum) Pada Tikus Putih (Rattus novergicus) Pricilia Onibala; Edwin De Queljoe; Olvie S Datu
PHARMACON Vol. 12 No. 1 (2023): PHARMACON
Publisher : UNIVERSITAS SAM RATULANGI

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Abstract

PRICILIA ONIBALA. Uji Efektivitas Antiinflamasi Salep Ekstrak Batang Pohon Pisang Ambon (Musa paradisiaca var. sapientum) pada Tikus Putih (Rattus novergicus). Dibawah bimbingan EDWIN DE QUELJOE sebagai ketua dan OLVIE S. DATU sebagai anggota.Batang pohon pisang ambon mengandung senyawa metabolit sekunder salah satunya ialah flavonoid yang merupakan senyawa penghambat antiinflamasi. Penelitian ini bertujuan untuk mengetahui efek antiinflamasi dari sediaan salep ekstrak batang pohon pisang ambon. Uji efektivitas antiinflamasi yang dilakukan dibagi dalam 5 kelompok perlakuan. Kelompok pertama (kontrol negatif) diberikan basis salep; kelompok ke-2 (kontrol positif) diberikan hidrokortison 2,5%; kelompok ke-3, ke-4, dan ke-5 diberikan salep ekstrak batang pohon pisang ambon dengan konsentrasi 10%, 20%, dan 40%. Masing-masing tikus diinduksi menggunakan karagenan 3% sebanyak 0,2 ml secara subkutan. Hasil penelitian menunjukkan bahwa kontrol negatif memiliki perbedaan yang signifikan dengan kelompok perlakuan lainnya dimana tidak menunjukkan efek antiinflamasi. Sebagai kesimpulan salep ekstrak batang pohon pisang ambon terbukti memiliki aktivitas antiinflamasi yang optimal pada dosis 40% yaitu 91,34% hampir sama dengan salep hidrokortison yaitu 96,24%.Kata Kunci: Antiinflamasi, ekstrak batang pohon pisang ambon, karagenan.
Co-Authors Abdullah, Surya Sumantri Adithya Yudistira Annisa Husain, Annisa Antasionasti, Irma Astrid Giovani Sitohang Bawotong, Repatri A BEIVY JONATHAN KOLONDAM Benny Wantouw Darenoh, Chelsy Datu, Olvie S. Edmont Hododjojo, Edmont Franco Luhulima Galomat, Debby E. Gosal, Angel T. Gultom, Minar N. S. Hanry J. Lengkong Henki Rotinsulu, Henki Herny E. I. Simbala Herny Simbala, Herny Holderman, Michelle V. Imbar, Agista Imbar, Agista C. Julianri Sari Lebang, Julianri Sari Kandou, Febby Kristin Yumte Ludong, Raynaldo M. Lydia Tendean Marina F. O. Singkoh Meivita Alfriani Sahensolar Michelle V. Holderman Mongi, Rifo Nangoy, Billy N. Nangoy, Billy Nathaniel Nur Alfan Muhammad Zen, Nur Alfan Muhammad Nurfitri, Mayang Marsina Obrien S Tendean, Obrien S Olivia H Abram Olvie S Datu Orpa Smarce Fransina Manori Pahlevi, Dylan R. Pandaleke, Siska Sefrida Parluhutan Siahaan Paulina V. Y. Yamlean Paulina yamlean Petta, Nabila S Pongoh, Adinda Fransisca Pricilia Onibala Purwoko, Agus Rambi, Christani Regina R. Butarbutar Rooije R.H. Rumende Roring, Tirza Sagay, Steleynes Saiful Hi. Umar, Saiful Hi. Saimina, Arie Saimina, Arie J. Samiun, Asriyani Saroyo . Sartje J. Rondonuwu Sedu, Anissa Sendy B. Rondonuwu Siampa, Jainer Pasca Simbala, Herny E. I. Singal, Ausich Suoth, Elly J. Surya Sumantri Abdullah Suryandari, Syari Sekar Tamimi, Alyah A.P Tamuntuan, Dhea N. Trina E Tallei Umboh, Defritsevani Umboh, Defritsevani Y. Vergina Claudia