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All Journal Dentika Dental Journal Journal of Mathematical and Fundamental Sciences STOMATOGNATIC- Jurnal Kedokteran Gigi Jurnal Kesehatan Gigi UNEJ e-Proceeding Dental Journal (Majalah Kedokteran Gigi) Health Notions Journal of Dentomaxillofacial Science AloHA International Journal of Health Advancement (AIJHA)
I Dewa Ayu Ratna Dewanti
Department Of Biomedical Science, Faculty Of Dentistry, Jember University, Jl. Kalimantan 37, Jember 68121
Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Nursing Physics Public Health

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Journal : Dental Journal (Majalah Kedokteran Gigi)

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TNF- α expression on rats after Candida albicans inoculation and neem (Azadirachta indica) extract feeding I Dewa Ayu Ratna Dewanti
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 1 (2011): March 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.166 KB) | DOI: 10.20473/j.djmkg.v44.i1.p49-53

Abstract

Background: Neem is a known traditional medicine from trees which function as immunomodulator. Candidiasis found in mouth is 80% caused by Candida albicans (C. albicans). Immunity is important to limit C. albicans since medicine price is relatively traditional medicine may become a good choice. In the other side the medicine price may not be reached by the citizen, cause citizen choose the traditional medicine. Purpose: The research is aimed to explain of TNF-α expression on rats after inoculated by C. albicans and fed with neem extract (Azadirachta Indica). Methods: There were 5 groups, the first group which was called as control group (KO) hadn’t been fed aqueous extract from neem leaves and was not inoculated by C. albicans, the other group (treatment) was classified into 4 groups. The first group was inoculated by C. albicans only (KP1), second group was fed with 50 mg/day/kg body weight aqueous extracts from Neem leaves, then inoculated with C. albicans starting from day 8 until day 21 (KP2), third group was fed with 100 mg/day/kg body weight aqueous extract from Neem leaves, then inoculated with C. albicans start from day 8 until day 21 (KP3), fourth group was fed with 200 mg/day/kg body weight aqueous extract from Neem leaves, then inoculated by C. albicans start from day 8 until day 21 (KP4). The data was collected from by swabbing the rat’s tongue to calculate C. albicans colonies. The rats were acclimated and collected for immunohistochemistry measurement. Results: The study showed that there were different result on ANOVA, HSD test, and linier regression. ANOVA showed significant difference (p < 0.01) between groups. The HSD test showed significant difference (p < 0.05) between each groups. TNF-α was the stimuli sensor from environment, and used as parameter to see the effect from the change of innate immunity component to C. albicans. Conclusion: Aqueous extract from neem leaves increased the macrophage TNF-α expression on in rat in oculated with C. albicans. Latar belakang: Mimba merupakan salah satu tanaman obat tradisional yang telah dikenal masyarakat dan berfungsi sebagai imunomodulator. Penyakit infeksi yang paling banyak dijumpai di rongga mulut (80%) adalah kandidiasis dengan penyebab utama Candida albicans (C. albicans). Di mana peran imunitas sangat penting pada C. albicans. Di sisi lain harga obat yang semakin mahal semakin tidak terjangkau masyarakat, menyebabkan masyarakat memilih obat tradisional. Tujuan: Riset ini untuk menjelaskan tentang ekspresi TNF-α makrofag pada tikus wistar yang diinokulasi C. albicans dan diberi konsumsi ekstrak cair daun mimba. Metode: Penelitian ini terbagi menjadi 5 kelompok, kelompok kontrol (KO) tidak diberi perlakuan, kelompok yang diinokulasi C. albicans (KP1), kelompok yang diberi konsumsi 50 mg/hari/kg dan diinokulasi C. albicans mulai dari hari 8 sampai hari 21 (KP2), kelompok yang diberi konsumsi 100 mg/hari/kg dan diinokulasi C. albicans mulai dari hari 8 sampai hari 21 (KP3), kelompok yang diberi konsumsi 200 mg/hari/kg dan diinokulasi C. albicans mulai dari hari 8 sampai hari 21 (KP4). Data dikumpulkan dari swabbing lidah untuk dihitung koloni C. albicans dan jaringan lidah dengan metode immunohistochemistry untuk penghitungan sel makrofag yang mengekspresikan TNF-α. Hasil: Studi menunjukkan terdapat perbedaan yang signifikan dari hasil ANOVA, uji HSD, regresi linier. ANOVA menunjukkan perbedaan (p < 0,01) antar kelompok. Uji HSD menunjukkan perbedaan (p < 0,05) antar kelompok. Hal ini dapat dikatakan bahwa TNF-α adalah sensor stimuli dari lingkungan, yang digunakan sebagai parameter untuk melihat pengaruh dari > perubahan dari komponen respons innate terhadap C. albicans. Kesimpulan: Ekstrak cair daun mimba dapa meningkatkan ekspresi makrofag TNF-α dari tikus yang diinokulasi C. albicans.
The role of Kuniran (U. moluccensis) and Gurami (O. goramy) fish thorns and scales in increasing salivary leukocyte and monocyte cells viability against Streptococcus mutans I Dewa Ayu Ratna Dewanti; I Dewa Ayu Susilawati; P. Purwanto; Pujiana Endah Lestari; Roedy Budirahardjo; Dyah Setyorini; Ristya Widi Endah Yani; Erawati Wulandari; Melok Aris Wahyukundari
Dental Journal (Majalah Kedokteran Gigi) Vol. 52 No. 1 (2019): March 2019
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1168.806 KB) | DOI: 10.20473/j.djmkg.v52.i1.p45-50

Abstract

Background: Kuniran thorns and Gurami fish scales are rich in protein and minerals such as dentin believed to increase cell viability against Streptococcus mutans (S. mutans) that causes dental caries. These, in turn, can cause systemic diseases if left untreated. Purpose: This study aims to analyze the influence of Kuniran thorns and Gurami fishes scales on the viability of monocytes and salivary leukocytes against S. mutans. Methods: Monocytes and leukocytes salivary cells were placed on a microtiter plate and treated according to the nature of each group. This study comprised the following groups: control group: untreated; S. mutans group: induced by S. Mutans; Gurami thorn group: thorns + S. Mutans; Gurami scales group: scales + S. Mutans; Kuniran thorn group: thorns + S. Mutans; Kuniran scales group: scales + S. mutans. Viability analysis involved staining with Tripan Blue. Furthermore, the number of viable cells (white) was calculated under an inverted microscope at 200 times magnification from five fields of view. Data was analyzed by means of an ANOVA test followed by LSD test. Results: The ANOVA and LSD tests confirmed significant differences (0.01<P). Kuniran thorns and Gurami fish scales increased the viability of monocytes and salivary leukocytes, but not significantly. The content of flavonoids, amino acids, omega 3, omega 6 and antioxidants increased leukocyte metabolism, thereby increasing cell resistance to S. mutans infection. Conclusion: Kuniran thorns (U. moluccensis) and Gurami (O. goramy) fish scales increase the viability of salivary leukocyte and monocyte cells against Streptococcus mutans.
Addition of gourami (Osphronemus goramy) fish scale powder on porosity of glass ionomer cement Erawati Wulandari; Farah Rachmah Aulia Wardani; Nadie Fatimattuzahro; I Dewa Ayu Ratna Dewanti
Dental Journal (Majalah Kedokteran Gigi) Vol. 55 No. 1 (2022): March 2022
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v55.i1.p33-37

Abstract

Background: Porosity is one of the disadvantages of glass ionomer cement (GIC) restorative materials, as it causes a reduction in strength and durability; the greater the porosity, the lower the strength of the restorative material and vice versa. As gourami fish scales contain calcium and phosphate, they have the potential to reduce the porosity of GIC. Purpose: This study aimed to analyse the effect of adding gourami fish scale powder (GFSP) on the pore size and porosity level of the GIC. Methods: This experimental research included a post-test-only control. The GFSP was fabricated using the freeze-drying method. Sixteen Fuji IX Extra sample cylinders with a diameter of 5 mm and a height of 3 mm were divided into four groups: K0, which comprised GIC without the addition of GFSP; K1, which contained GIC powder + 2.5% GFSP (by weight); K2, which comprised GIC powder + 5% GFSP (by weight), and K3, which contained GIC powder + 10% GFSP (by weight). The samples were observed using scanning electron microscopy and measured using ImageJ software. Data were analysed using a one-way analysis of variance (ANOVA) test. Results: The addition of 2.5% GFSP (by weight) produced the smallest pore size and lowest porosity, while the one-way ANOVA test results were significant among all groups at p = 0.000. There was no significant difference in pore sizes between K0 and K1 (p = 0.359), but a significant difference was found in the level of porosity (p = 0.024). Conclusion: The addition of GFSP affected the porosity of the GIC; the pore size and porosity level of the GIC were reduced by the addition of 2.5% GFSP.
Co-Authors Depi Praharani Dheamira Rosida Dyah Setyorini Erawati Wulandari Farah Rachmah Aulia Wardani Fiona Budi Amarta Domini Hestieyonini Hadnyanawati I Dewa Ayu Susilawat I Dewa Ayu Susilawati Melok Aris Wahyukundari Nadie Fatimatuzzahro Narita Ajeng Loviana Nuzulul Hikmah P. Purwanto Pudji Astuti Pujiana Endah Pujiana Endah Lestari Purwanto Purwanto , Ristya Widi Endah Lestari Ristya Widi Endah Yani Roedy Budirahardjo Sri Lestari Sunana Ageng Hikmawati Sunlip Wibisono