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All Journal Jurnal Veteriner Jurnal NERS Majalah Obstetri dan Ginekologi The Indonesian Biomedical Journal Jurnal Penelitian Kesehatan Indian Journal of Forensic Medicine & Toxicology
Hendy Hendarto
Departemen Obstetri Dan Ginekologi, Fakultas Kedokteran, Universitas Airlangga
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Indian Journal of Forensic Medicine

 1 
Curcumin Improves the Regulation of Ovarian Folliculogenesis in Culture Media with Peritoneal Fluid from Infertile Women with Endometriosis Hendy Hendarto1, M Yohannes Ardianta Widyanugraha1, Widjiati2, Soehartono Darmosoekarto1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.3154

Abstract

Background : Peritoneal fluid (PF) from infertile women with endometriosis contains a variety of inflammatory mediators that may interfere with folliculogenesis. The aim of the study was to evaluate effects of curcumin on the regulation of ovarian folliculogenesis by evaluating Growth Differentiation Factor (GDF)-9, Kit Ligand (KitL) and Tumor Necrosis Factor (TNF)? expressions in bovine cumulus oocyte complexes (COC)s cultured with PF from infertile women with endometriosis. Method: COCs were aspirated from antral follicles of bovine ovaries. PF was collected from infertile women with endometriosis undergoing laparoscopy for infertility evaluation. Curcumin, a strong antiinflammatory turmeric, was added in Tissue Culture Media (TCM)199 and PF from infertile women with endometriosis for culture media. Bovine COCs were cultured into 3 groups of media: 1) TCM199, 2) TCM199+PF from infertile women with endometriosis, and 3) TCM199+PF from infertile women with endometriosis+curcumin. GDF-9, KitL and TNF? expressions were examined using immunohistochemistry technique. Results: GDF-9 expression of bovine COCs cultured in PF from infertile women with endometriosis with curcumin addition (2.67±0.98) was found to increase compared to those cultured without curcumin (0.50±0.67) but reduced compared to the control. This result was similar to KitL expression of bovine COCs cultured with curcumin (2.67±1.23), in which it increased compared to those without curcumin (0.33±0.49) (p<0.05). A significant difference in TNF? expression was noted between groups with or without curcumin (p<0.05). Conclusion: In the culture of PF from infertile women with endometriosis, curcumin addition may improve the regulation of ovarian folliculogenesis through the decrease of inflammation factor.
Moringa Leaf (Moringa oleifera Lam) Nanoparticle Supplementation on Zygote Cleavage in Goat Embryo Culture In Vitro Alfina Aisatus Saadah1 , Widjiati2 , Hendy Hendarto3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.10669

Abstract

One of the causes of failure of zygote development is oxidative stress in the culture medium. Where oxidative stress can damage the structure and function of zygotes due to free radicals Reactive Oxygen Species (ROS). Moringa oleifera Lam contains antioxidant polyphenol compounds, flavonoid flavanols namely quercetin which is the most antioxidant content in Moringa leaves which is expected to fight excess ROS. The purpose of this study was to prove that the supplementation of Moringa oleifera Lam leaf nanoparticles to increase zygote cleavage in goat embryo culture in vitro. The research subjects used goat oocytes which were divided into 4 groups consisting of 1 control group and 3 treatment groups. Each group consisted of 31 samples. Control group K1 without Moringa leaf nanoparticle supplementation, treatment group I P1 Moringa leaf nanoparticle supplementation with a dose of 0.5 µM, treatment group II P2 supplementation of Moringa leaf nanoparticles with a dose of 1.0 µM, Treatment group III P3 supplementation with Moringa leaf 2.0 µM. Embryo culture was carried out in a 5% CO2 incubator, temperature 38.5ºC for 48 hours, then observed under an inverted microscope. The results show that there are significant differences with the value of p = 0.041 which means p <0.05. Supplementation of moringa leaf nanoparticles (Moringa oleifera Lam) can significantly increase zygote cleavage at embryo culture stage in vitro.
Nanocurcumin Potential Effect of SOD Enzyme and Caspase-3 Expression in Lead-Acetate Induced Rats Ovarian Granulosa Cells Anis Satus Syarifah; Sri Agus Sudjarwo; Hendy Hendarto; Reny I’tishom; Supriyanto
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14650

Abstract

Aim: This study investigated the potential effect of nanocurcumin on the increase of SOD enzyme expressionand decrease of caspase-3 in lead acetate-induced rats ovarian granulosa cells.Materials and Methods: Forty five female rats were divided into 5 groups, the negative control group (ratsreceiving corn oil, one hour later receiving distilled water), positive control group (rats receiving corn oil,one hour later receiving lead acetate of 40 mg/kg bw) and experimental groups 1, 2 and 3 (rats receivingnanocurcumin 50 mg, 100 mg and 200 mg/kg bw). One hour after administering the nanocurcumin, therats received 40 mg/kg of lead acetate. All groups received oral treatment once a day for 26 days. Onday 27 the rats were sacrificed and the expression of SOD and caspase-3 enzymes were measured usingimmunohistochemical methods.Results: This study found that lead acetate decreased SOD enzyme expression and increased caspase-3. Incontrast, nanocurcumin increased SOD enzyme and decreased caspase-3 expression in lead acetate-inducedrats ovarian granulosa cells.Conclusion: Nanocurcumin has potential as a strong natural antioxidant by affecting the increase of SODand the decrease of caspase-3 cells in lead acetate-induced rats ovarian granulosa cells.
The Relationship of Antral Follicle Count (AFC) with Levels of Estradiol Serum and Mature Ovarian Follicles in Women Undergoing the IVF Program at the Graha Amerta Fertility Clinic of General Hospital of Dr. Soetomo in the Period 2018 Anak Agung Renjani Kanya Kumari; RenyI’tishom; Hendy Hendarto; Sri Ratna Dwiningsih
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15608

Abstract

Background: In the last two decades, one of the critical factors of assisted reproductive technology (ART)has been identified as ovarian capacity. Consequently, ovarian capacity markers have evolved to be part ofdiagnostic testing conducted regularly before IVF. Several predictor factors, such as antral follicle count(AFC), is one of several factors to predict ovarian capacity. These diagnostic procedures and tests have thepurpose of identifying women at high-risk patient who respond poorly toward controlled ovarian stimulationthroughout the IVF cycle. The ovarian response toward ovulation stimulation within this research was assessedby peak estradiol (E2) serum levels and the total number of mature follicles. There is no clear relationshipbetween AFC and levels of estradiol serum and the number of mature ovarian follicles to identify womenwho respond poorly. Methods: This study is an analytical study with a cross-sectional design. Samples weretaken from secondary data of medical records using the total sampling method on women undergoing the IVFprogram at the Graha Amerta fertility clinic of General Hospital of Dr. Soetomo from January 1 to December31 2018 according to the inclusion and exclusion criteria. The AFC variables, estradiol serum levels, and thenumber of mature ovarian follicles in the study were analyzed using Spearman’s correlation. Conclusion:There is a significant relationship between antral follicle count (AFC) with the levels of estradiol serum andthe number of mature follicles in women undergoing the IVF program.
Co-Authors Anak Agung Renjani Kanya Kumari Ashon Sa’adi Dian Irawati Faroek Hoesin Hening Laswati I'tishom, Reny Juminten Saimin Laba Mahaputra MY Ardianta W Nur Anindya Syamsudi Raudatul Hikmah Soetjipto Soetjipto Sri Agus Sudjarwo Sri Ratna Dwiningsih Syarifah, Anis Satus Tinta Julianawati Widjiati w Widjiati Widjiati Widjiati Widjiati Widjiati Widjiati Yulisa Haslinda