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Muhammad Anwar Djaelani
Departemen Biologi, Fakultas Sains Dan Matematika, Universitas Diponegoro Jl. Prof. Soedarto, SH, Tembalang, Semarang, Jawa Tengah, Indonesia 50275
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Rasio Otot-Tulang Ekstremitas Posterior Ayam Pedaging Setelah Pemberian Teh Kombucha Dalam Air Minum Kumala, Amelia Setya Nur; Djaelani, Muhammad Anwar; Yuniwarti, Enny Yusuf Wachidah
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.182 KB) | DOI: 10.14710/bioma.19.1.62-68

Abstract

Kombucha tea beverage obtained by fermenting sweetened green tea for 12 days with Acetobacter xylinum and Saccharomyces which produce various kinds of organic acids, vitamins, and acts as a probiotic. Kombucha tea role as growth promoter is to enhance the metabolic processes in the digestive system of broiler, so that the nutrients can be absorbed and fulfilled properly for optimal growth and development. The research was conducted from September to October 2014 in the Laboratory of Biological Structure and Function, Diponegoro University. Posterior limb (femur-tibiotarsus) meat-bone ratio analysis was used to investigate the effects of kombucha tea on the productivity of broiler chicken. A total of 20 broiler chickens were randomly divided into 4 treatment concentrations ( 0%, 10%, 20%, and 40% of kombucha tea in drinking water) for 32 days. Statistical analysis using ANOVA at 0,05 significance level and Duncan’s Multiple Range Test showed significantly different results on body weight and water intake, while feed intake, meat weight, bone weight and meat-bone ratio showed no significant results. It can be concluded from this study that the decrease of broiler chicken body weight due to consumption of 40% kombucha tea concentration in drinking water, do not cause a decrease in posterior limb meat and bone weight so that the posterior limb meat-bone ratio were not significantly different. Key words: Kombucha tea, broiler chicken, posterior limb, meat-bone ratio.
Efek Pemberian Virgin Coconut Oil Dan Olive Oil Dalam Pakan Terhadap Pertumbuhan Tulang Femur Tikus Putih Jantan (Rattus norvegicus) Suhartatik, S; Yuniwarti, Enny Yusuf Wachidah; Djaelani, Muhammad Anwar
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 2, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (48.015 KB) | DOI: 10.14710/bioma.19.2.89-94

Abstract

This research aimed to annalyse the effects of virgin coconut oil and olive oil on the length, diameter and weight of the femur bone in white male rat (Rattus norvegicus). This research used 25 white male rats and consists of 5 treatments with 5 replications. This research used the treatment P0: as a control, P1: feed with an addition of 5 ml virgin coconut oil / kg feed, P2: feed with an addition of 10 ml virgin coconut oil / kg of feed, P3: feed with an addition of 5 ml olive oil / kg feed and P4: feed with  an addition of 10 ml olive oil / kg feed. This research used a method called Completely Randomized Design (CRD). The variables measured in this research are the weight, length and diameter of white male rats. The data was analyzed using Analysis of Variance (ANOVA) at 95% level. The results showed that the growth of femur bone in male rat does not have different response after giving virgin coconut oil and olive oil.  
Profil Histologi Duodenum Berbagai Itik Lokal Di Kabupaten Semarang Apriliyani, Nur Indah; Djaelani, Muhammad Anwar; Tana, Silvana
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (53.441 KB) | DOI: 10.14710/bioma.18.2.144-150

Abstract

Local duck had been widely cultivated not only as a producer of eggs processed as salted egg but also there are cultivated as broiler ducks. The efforts to improve the productivity of ducks can be seen from the effectiveness of the process of absorption of nutrients in the duodenum, so the use of energy in the metabolic process can be optimized for adding mass and volume of the cell.  This study aims to determine the duodenum profile of the histological structure  covered the lumen diameter, villous height and number of goblet cells of Magelang ducks, Tegal ducks and Pengging ducks .This study used a completely randomized design (CRD) with three treatments (Magelang ducks, Tegal ducks and Pengging ducks) and 6 replications. The measured variable was the long intestine tenue, lumen diameter, villous height and number of goblet cells. Data were analyzed using analysis of variance (ANOVA) with a differentiation factor was the type of duck. The realdifferences data were analyzed using advanced test of Duncan at the level of 95%. The results showed long intestine tenue, lumen diameter and height of the villi were not significantly different at each Magelang ducks, Tegal ducks, Pengging ducks. The number of goblet cells in Magelang ducks and Tegal ducks showed significantly different, but not on Pengging Ducks. The conclusion of the study was the length of the intestine tenue, lumen diameter and height of villous had no real effect on three types of ducks. Keywords: Local ducks, duodenum, histological
Bobot, Indeks Kuning Telur (IKT), Dan Haugh Unit (Hu) Telur Ayam Ras Setelah Perlakuan Dengan Pembungkusan Pasta Rimpang Temulawak (Curcuma xanthorrhiza Roxb). Putri, Dyah Ayu Maharani; Djaelani, Muhammad Anwar; Mardiati, Siti Muflichatun
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.2 KB) | DOI: 10.14710/bioma.18.2.7-13

Abstract

Eggs are farm products that provide the greatest contribution to the achievement of community nutritional adequacy. The aims of the research was to an analyzed the quality of eggs in treatment without and with a temulawak rhizome pasta stored at 6,12, and 24 days. This research used  35 eggs consists of 7 treatment and 5 replication, namely PtT0 : as control, a group of eggs without packing temulawak rhizome pasta and without storage, PtT6 : a group of eggs without packing temulawak rhizome pasta after 6 days storage, PdT6 : a group of eggs with packing temulawak rhizome pasta after 6 days storage, PtT12 : a group of eggs without packing temulawak rhizome pasta after 12 days storage, PdT12 : a group of eggs with packing temulawak rhizome pasta after 12 days storage, PtT24 : a group of eggs without packing temulawak rhizome pasta after 24 days storage, and PdT24 : a group of eggs with packing temulawak rhizome pasta after 24 days storage. This research was analyzed used a completely randomized design (CRD). Variables measured were egg weight, yolk indeks, and Haugh Unit (HU). Data obtained from this study were analyzed using analysis of variance (ANOVA) if there is a real difference then continued further test using Duncan test at the level 95 %. The result  egg weight reducted and yolk index shows was significantly different in PtT6, PtT12, PtT24, and PdT24 and Haugh Unit (HU) shows was significantly different in PtT6, PtT12, PtT24. Conclusion of this research is gived temulawak rhizome pasta are able to maintain the quality of eggs were observed on days 6, 12, and 24. Key Word : Chicken egg,  Temulawak rhizome, Egg quality
Kualitas Telur Puyuh Jepang (Coturnix coturnix japonica L.) berdasarkan Variabel pH telur, Indeks Kuning Telur (IKT) dan Indeks Putih Telur (IPT) setelah Penyimpanan yang Dilakukan Perendaman Pada Air Kapur Sebelum Penyimpanan Djaelani, Muhammad Anwar
Bioma : Berkala Ilmiah Biologi Vol. 20, No. 1, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.455 KB) | DOI: 10.14710/bioma.20.1.31-34

Abstract

Puyuh Jepang (Coturnix coturnix japonica L.) banyak dibudidayakan untuk diambil telurnya karena produktivitas telur yang tinggi. Nilai gizi telur puyuh tidak kalah dengan telur unggas lain, sehingga dapat menambah variasi dalam penyediaan sumber protein hewani. Telur merupakan salah satu bahan makanan yang hampir sempurna. Bahan makanan ini mengandung zat gizi lengkap antara lain protein, lemak, vitamin dan mineral. Telur segar yaitu telur yang baru diletakkan oleh induk disarangnya, mempunyai daya simpan yang pendek. Salah satu faktor yang menyebabkan rusaknya telur adalah lama waktu penyimpanan . Penelitian ini bertujuan untuk menganalisis kualitas telur puyuh berdasarkan pH Telur,  nilai Indeks Kuning Telur (IKT), Indeks Putih Telur (IPT) setelah perendaman dengan air kapur. Sampel yang digunakan adalah telur puyuh yang diambil pada hari kedua peneluran. Digunakan Rancangan acak  lengkap dengan 5 kelompok perlakuan yaitu P1 kelompok telur,diamati pada hari ke 2. P2 kelompok telur disimpan dan diamati pada hari ke 16. P3 kelompok telur direndam pada air kapur selama 1 menit, disimpan dan diamati pada hari ke 16. P4 kelompok telur disimpan dan diamati pada hari ke 30. P5 kelompok telur direndam pada air kapur selama 1 menit, disimpan dan diamati pada hari ke 30. Data yang diperoleh dianalisis menggunakan ANOVA dengan taraf signifikansi 5%. Hasil penelitian menunjukkan semua variabel penelitian menenunjukkan penurunan seiring dengan bertambahnya waktu penyimpanan. Penurunan kualitas disebabkan faktor lamanya penyimpanan yang menyebabkan perubahan kondisi telur. Kata kunci : Penyimpanan telur, pH telur, Indeks Kuning Telur, Indeks Putih Telur
Motilitas dan vitalitas spermatozoa manusia post freezing setelah simpan beku dengan medium TES-Tris yolk citrat yang dimodifikasi dengan jenis kuning telur berbeda Djaelani, Muhammad Anwar
Bioma : Berkala Ilmiah Biologi Vol. 14, No.1, Tahun 2012
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (60.81 KB) | DOI: 10.14710/bioma.14.1.37-41

Abstract

The aim of this research was to examine the possibility of semen cryopreservation using modified TES-Tris yolk citrat (TES-TYC) medium with different egg yolk. Semen fulfilling inclusion  criteria with WHO  criteria was divided into three groups and the sperm motility and vitality was counted as initial data. The semen was then mixed with TES-TYC medium with chicken egg yolk, TES-TYC medium with duck egg yolk and TES-TYC medium with goose egg yolk then cryopreserved in liquid nitrogen. After one mounth the semen was thawed and recount its sperm motility and vitality. Data obtained showed that the motility and vitality of  post freezing sperm cryopreserved with TES-TYC medium with chiken was higher compared to the other medium. It could be concluded that the existenced of chicken egg yolk in TES-TYC medium was better kept sperm integrity during cryopreservation compared with the other medium, hence the existenced of egg yolk as ingredient in TES-TYC medium should be chicken egg yolk.   Key words : motilitas dan vitalitas, medium TES-Tris yolk citrat, kuning telur
Studi Awal Upaya Eksplorasi Agensia Imunokontrasepsi Untuk Regulasi Fertilitas Hewan Liar : Pofil Protein Selama Proses Implantasi Embrio Mencit (Mus musculus L.) BALB/c. Sitasiwi, Agung Janika; Djaelani, Muhammad Anwar
Bioma : Berkala Ilmiah Biologi Vol. 13, No. 1, Tahun 2011
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (95.169 KB) | DOI: 10.14710/bioma.13.1.39-45

Abstract

Study on exploration of immunocontraception agents for wildlife fertility regulation has been conducted.The aim of this study was carried to find out the protein which functionable in mice embryos implantation. Theoutcome of this study should be applied to control the fertility of wildlife animals. This research conducted duringten months in BSF Laboratory of Biology Department FMIPA UNDIP and 3rd Unit of UGM LPPT. Adult femaleand male mice with 28 – 30 grams in weight were used as laboratory animals. Mice were divide into two groups, onegroup as positive control group without mated, the other were mated group. Mice handled and breeded in laboratorycondition. The precise day of pregnancy were determined with vaginal plug existences in female mice. Protein wereisolated from uterus on day 1st to 5th of pregnancy. Protein isolation and separation with electrophoresis were basedon Bio-rad manual. This study show that there are no differences in protein band between positive control group andpregnant group. These study suggested that protein determining embryo implantation process is regulatory proteinwhich it has expressed in short time and fast withdrawl.
Pelacakan Gen Sitokrom Oksidase Sub Unit I (COI) DNA Mitokondria Itik Tegal (Anas domesticus) Menggunakan Primer Universal Wibowo, Sarwo Edi; Djaelani, Muhammad Anwar; Kusumaningrum, Hermin Pancasakti
Bioma : Berkala Ilmiah Biologi Vol. 15, No.1, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.965 KB) | DOI: 10.14710/bioma.15.1.20-26

Abstract

Currently local ducks are generally quite difficult to find in a big farm in Inonesia, including Tegal ducks. Tegal ducks is one of the genetic resources native to Indonesia with it’s advantages in terms of high and large egg production. Conservation and development of local ducks have strived to maintain our existence of Indonesian livestock germplasm. If such information is not superior to native species exist, the opportunity to increase his lead further also getting smaller. Tracking the mitochondrial COI gene DNA of Tegal ducks may underlie the process of an organism's genetic characterization. Information about Tegal duck mitochondrial DNA has not been done. The information obtained can be used for optimization of duck products native to Indonesia both in physiological aspects, phylogeny and genetic engineering. The research method used in this research is tracking COI gene data from Gen Bank with the programs Clustal X and Genedoc. Tracking then continued using universal primers HCO and LCO. The results of the data followed up with the isolation and amplification of COI gene mitochondrial DNA as well as the optimization of PCR conditions. The results showed mitochondrial DNA COI gene Tegal ducks were amplified with primer LCO obtain DNA fragments of length less than 250 bp. Kata kunci: duck’s from Tegal, COI gene, mitochondrial DNA
Profil Kolestrol Darah Tikus Setelah Pemberian Virgin Coconut Oil Dan Minyak Zaitun Djaelani, Muhammad Anwar
Bioma : Berkala Ilmiah Biologi Vol. 17, No.2, Tahun 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.696 KB) | DOI: 10.14710/bioma.17.2.102-105

Abstract

Tingginya kadar kolestrol dalam tubuh menjadi pemicu munculnya berbagai penyakit. Masyarakat berusaha menurunkan kadar kolesterol dalam tubuhnya. Komponen VCO berupa asam lemak jenuh sekitar 90% dan asam lemak tak jenuh sekitar 10%. Asam lemak jenuh VCO didominasi oleh asam laurat. VCO mengandung  asam laurat dan asam kaprilat. Keduanya merupakan asam lemak rantai sedang yang biasa disebut Medium Chain Fatty Acid (MCFA).  Minyak zaitun atau Olive oil mengandung lemak tak jenuh yang tinggi terutama asam oleat dan polifenol. Polifenol merupakan  antioksidan  yang  dapat  mengikat LDL teroksidasi. Penelitian ini bertujuan untuk mengkaji efek asam lemak jenuh yang menggunakan VCO dan efek asam lemak tak jenuh yang menggunakan minyak zaitun terhadap profil kolesterol darah pada tikus putih. Penelitian ini menggunakan Tikus putih jantan sebanyak 25 ekor yang dibagi dalam 5 kelompok perlakuan yaitu P1 adalah kelompok kontrol, P2 kelompok yang diberi VCO 5 mL/kg pakan, P3 kelompok yang diberi VCO 10 mL/kg pakan, P4 kelompok yang diberi minyak zaitun 5 mL/kg pakan dan P5 kelompok yang diberi minyak zaitun 10 mL/kg pakan. Tiap perlakuan diulang sebanyak 5 kali. Perlakuan selama 4 minggu. Variabel yang diamati meliputi kadar Kolesterol total, HDL dan LDL darah tikus. Data yang didapat, dianalisis menggunakan ANOVA. Hasil yang didapat menunjukkan pada perlakuan  dengan VCO menunjukkan pengaruh yang tidak signifikan terhadap perubahan ketiga variabel penelitian. Pada perlakuan  dengan minyak zaitun kadar HDL tidak terdapat perbedaan yang signifikan antar kelompok perlakuan, kadar Kolesterol total pada kelompok perlakuan menunjukkan penurununan yang tidak signifikan dan kadar LDL pada kelompok perlakuan 10 mL/kg pakan menunjukkan penurununan yang signifikan. Dapat disimpulkan bahwa pemberian VCO tidak berpengaruh pada profil kolestrol darah tikus normolipidemia, dan minyak zaitun  berpotensi menimbulkan respon penurunan profil kolesterol tikus pada kadar ≥10 mL/kg pakan.   Kata kunci: tikus putih, Kolesterol total, HDL dan LDL, Minyak Zaitun, VCO.
PEMBERIAN TEH KOMBUCHA PADA AIR MINUM TERHADAP NILAI LDL KOLESTEROL DAN HDL KOLESTEROL DARAH AYAM BROILER (Gallus sp) Djaelani, Muhammad Anwar; Tana, Silvana
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 23, No 2 (2015): Volume XXIII, Nomor 2, Oktober 2015
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (390.371 KB) | DOI: 10.14710/baf.v23i2.10017

Abstract

The risk of consuming large quantities of food containing cholesterol has been widely known. By knowing the cholesterol content of food products, people could restrict their consumption of high cholesterol food. This study was to knew LDL cholesterol and HDL cholesterol of blood broiler chickens after treated with kombucha tea. This research used the CP 707 broiler strains chickens aged 1 week, treated with kombucha tea that has been fermented for 12 days at a temperature of 25oC. 20 broiler chickens devided into 4 treatment groups ( treated for 4 weeks), each group contain 5 replications. That group was : P0 as control group, drinking water without  kombucha tea, P1 group with  drinking water added with 10% kombucha tea, P2 group with  drinking water added with 20% kombucha tea, P3 group with  drinking water added with 40% kombucha tea. Variables measured were LDL cholesterol of blood chicken levels, HDL cholesterol of blood chicken levels and chicken drink consumption. Data were analyzed with Anova. The results showed that additional of kombucha tea in drinking water able to lower LDL cholesterol of blood chicken levels and able to increase  HDL cholesterol of blood chicken levels significantly of broiler chickens. Keywords: broiler chickens, kobucha tea, cholesterol
Co-Authors Agata Rio Pratama Agung Janika Sitasiwi Agung Janika Sitasiwi Apriliyani, Nur Indah Arief Niendya W Arif Nur Latifah Dara Puspita Jati Windoro Dewi Purwaningsih Dwi Rahmawati Dyah Ayu Maharani Putri, Dyah Ayu Maharani Enny Yusuf Wachidah Yuniwarti Enny Yusuf Wachidah Yuniwarti Evi Lestari Fernanda Imansari Hermin Pancasakti Kusumaningrum Hikmah Putri Pratiwi K Kasiyati Kasiyati Kasiati Kasiyati, _ Kencana Ayudya Prabahandari Khunfaya Firnanda Putri Rossida Kumala, Amelia Setya Nur Lia Lestari Luthfiana Ulil Albab Luthfiana Ulil Albab Masadah, Siti Murni Muhammad Alvin Gibran Novia Marcelina Nur Azizah Nur Azizah Rahita Dayurani Salsabila Alifah Sarwo Edi Wibowo, Sarwo Edi Sella Widya Astuti Silvana Tana Siska Hairunnisa Siti Muflichatun Mardiati Siti Murni Mas'adah SRI ISDADIYANTO Suhartatik, S Sunarno Sunarno Sunarno Sunarno Sunarno, _ Susan Nuraeni Teguh Suprihatin Tyas Rini Saraswati Zulfa, Laili Fitria Zuni Novika Zuni Novika