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Sunarno Sunarno
Biology Laboratory Structure And Function Of Animals, Department Of Biology, Faculty Of Science And Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH, Tembalang, Semarang
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Engineering Health Professions Medicine & Pharmacology Public Health Social Sciences Veterinary

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Analisis Efek Pemeliharaan Sistem Intensif dan Ekstensif terhadap Produktivitas dan Kualitas Telur Itik Tegal Sunarno Sunarno; K. Budiraharjo; Solikhin Solikhin
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 23, No 2 (2021): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.23.2.83-93.2021

Abstract

Itik (Anas plathyrynchos domecticus) merupakan salah satu jenis unggas yang banyak dibudidayakan, terutama sebagai penghasil telur. Produk telur diketahui bermanfaat dalam pemenuhan kebutuhan protein hewani bagi masyarakat di Indonesia. Permasalahan yang dihadapi oleh peternak itik petelur, terutama di Kabupaten Brebes adalah pakan, keterbatasan pengetahuan tentang pemeliharaan itik, permintaan telur asin oleh konsumen yang tinggi yang tidak diiringi oleh peningkatan kualitas dan kuantitas telur yang diproduksi itik. Penelitian ini dilakukan untuk menganalisis pengaruh sistem pemeliharaan itik secara intensif dan ekstensif terhadap kuantitas dan kualitas telur. Variabel kualitas telur meliputi bobot badan, produksi dan bobot telur, adapun variable kualitas telur terdiri atas pH dan tinggi albumen, pH dan diameter yolk, indeks kuning telur (IKT), dan Haugh Unit (HU). Metode penelitian yang digunakan adalah pembandingan 2 populasi, yaitu antara itik yang dipelihara dengan sistem intensif dan ekstensif, masing-masing menggunakan itik sebanyak 25 ekor. Data yang didapat dianalisis dengan menggunakan uji t (t student test) pembandingan dua perlakuan. Analisis hasil penelitian menunjukkan, itik yang dipelihara dengan sistem intensif berbeda nyata dengan sistem ektensif, serta memiliki kuantitas dan kualitas telur yang lebih tinggi. Kesimpulan dari penelitian ini adalah bahwa pemeliharaan itik secara intensif dapat meningkatkan kuantitas dan kualitas telur itik, lebih tinggi dibanding itik yang dipelihara secara ekstensif. Pemeliharaan itik secara intensif direkomendasikan dapat diaplikasikan oleh para peternak lokal di Kabupaten Brebes dengan pemanfaatan sumber bahan pakan lokal yang mudah diperoleh, murah, dan aman sehingga dapat meningkatkan pendapatan peternak.
Respons Hematologi Itik Pengging yang Diberi Tepung Daun Kelor (Moringa oleifera. Lam) Sebagai Imbuhan Pakan Kasiyati Kasiyati; Muhammad Anwar Djaelani; Sunarno Sunarno
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.436 KB) | DOI: 10.19087/jveteriner.2021.22.1.8

Abstract

Pengging ducks are one breed of Indonesia’s local duck has superior potential. This study was designed to evaluate the hematological parameters of Pengging ducks fed Moringa leaf meal as a feed additive. The data were obtained from a total of 80 female Pengging ducks of 35 weeks old were assigned into a completely randomized design and each experimental unit consisted of 4 replications. The treatment group consisted of basal fed without added Moringa leaf meal (control), basal fed added with 2.5, 5, 7.5, and 10% Moringa leaf meal. Data analyzed using one-way ANOVA. The results showed that the addition of Moringa leaf meal gave same effect (P>0.05) on the number of erythrocytes, hemoglobin, hematocrit, mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH). However, the mean corpuscular hemoglobin concentration (MCHC) increased (P<0,05) to 15.91% at 5% moringa leaf meal. The number of leukocytes was lower (P<0.05) by 42.01% at 7.5% moringa leaf meal. Leukocyte differentials, the ratio of H:L, and trombocytes did not significant difference (P>0.05) between treatments. In conclusion, the Moringa leaf meal as a feed additive did not change the hematological parameters and could be increasing the health status of layer Pengging ducks.
Potential of Glutathione Antioxidant in the Hippocampus Repair: Preliminary Study on Bioactive Materials Antiaging of Snakehead Fish (Channa striata) in Animal Models of Aging Sunarno Sunarno
International Journal of Science and Engineering Vol 8, No 1 (2015)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.461 KB) | DOI: 10.12777/ijse.8.1.22-25

Abstract

Snakehead fish meat contains active ingredients with anti-aging potential that serves as a precursor of glutathione. The ability of glutathione as an antiaging opportunities in the utilization of fish meat, especially snakehead fish. Snakehead fish meat contains several important amino acids, such as glutamine, cysteine​​, and glycine so the potential to be developed for the production of food that is nutritious and healthy. This study examines the essential amino acid composition of the antioxidant glutathione precursors found in snakehead fish from Rawa Pening Central Java to increase glutathione in the body and brain. The results showed that every 100g of snakehead fish meat from Rawa Pening containing glutamine (32.39%), cysteine ​​(6.61%), and glycine (9.69%). Snakehead fish meat extract given at a dose of 30 ml/kg/day in both types of animal models of aging effect on the increase in the content of glutathione and glutathione precursors, both in blood and hippocampus. Increased glutathione precursor of the most high to low, respectively glutamine, glycine, and cysteine​​. Availability of essential amino acids can support increased glutathione in the brain. This is indicated by an increase in glutathione hippocampus in both animal models, both on chronological aging or aging due to oxidative stress, respectively (0.822 and 0.359) mol/g bb compared to control tissue.
ISOLASI DAN KARAKTERISASI BAKTERI ENDOFIT PENGHASIL INHIBITOR α-GLUKOSIDASE DARI TANAMAN PARE (Momordica Charantia L) Sri Pujiyanto; Sunarno Sunarno; Annisa Widyasari
Prosiding SNST Fakultas Teknik Vol 1, No 1 (2015): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 6 2015
Publisher : Prosiding SNST Fakultas Teknik

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diabetes merupakan penyakit metabolik yang serius di Indonesia. Jumlah penderita diabetes mengalami peningkatan setiap tahunnya. Inhibitor α-glukosidase merupakan senyawa yang dapat menghambat enzim α-glukosidase sehingga bermanfaat sebagai obat antidiabetes. Tanaman Pare (Momordica charantia) diketahui memiliki kasiat sebagai anti diabetes. Senyawa aktif yang dihasilkan tanaman tersebut dapat berasal dari endofit yang hidup di dalam jaringan tanaman. Eksplorasi bakteri endofit dari tanaman pare merupakan salah satu cara untuk mendapatkan isolat bakteri penghasil inhibitor enzim α-glukosidase. Isolasi bakteri dilakukan dengan menginokulasikan  sampel tanaman yang telah disterilisasi permukaan pada media agar. Isolat bakteri yang didapat selanjutnya dimurnikan dan di karakterisasi lebih lanjut baik morfologi maupun aktivitas inhibitor α-glukosidasenya. Pengujian aktivitas inhibitor α-glukosidase menggunakan metode spektrofotometer dengan nitrofenil-α-D-glukopiranosida sebagai substrat. Hasil penelitian diperoleh  5 isolat bakteri endofit penghasil inhibior α-glukosidase. Penghambatan terbesar ditunjukkan oleh isolat bakteri Ad 1 yaitu sebesar 27,4% . Kata kunci: antidiabetes, Momordica charantia, bakteri endofit, inhibitor α-glukosidase
POTENSI DAGING IKAN GABUS (CHANNIA STRIATA) SEBAGAI SUPLEMEN PAKAN DAN PERANANNYA DALAM PEMULIHAN STATUSHEMATOLOGIS TIKUS WISTAR YANG DIBERI STRES Sunarno Sunarno; Siti Muflichatun Mardiati; Endang Nawarikita
Bioma : Jurnal Ilmiah Biologi Vol 6, No 1 (2017): Bioma
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v6i1.1468

Abstract

Stres karena kekurangan nutrisi yang disertai aktivitas berlebihan dapat menyebabkan defisiensi energi dalam tubuh. Kondisi ini dapat berakibat pada terganggunya proses pembentukan eritrosit, sintesis hemoglobin, dan penurunan bobot badan. Penelitian bertujuan menganalisis pengaruh pemberian suplemen daging ikan gabus (Channia striata) dalam pakan terhadap peningkatan jumlah eritrosit, kadar hemoglobin, dan bobot badan hewan uji yang diberi stres. Penelitian ini menggunakan hewan uji tikus Wistar jantan. Desain eksperimen menggunakan rancangan acak lengkapyang terdiri atas 5 perlakuan dan 4 kali ulangan. Perlakuan terdiri atas P0:  tikus kontrol (tikus tanpa diberi stres dan tanpa suplemen daging ikan gabus dalam pakan); P1, P2, P3, dan P4 berturut-turut adalah tikus diberi stres dan suplemen daging ikan gabus dalam pakan dengan konsentrasi 5%; 10%; 15%; dan 20%. Variabel yang diukur pada penelitian ini adalah jumlah eritrosit, kadar hemoglobin, dan bobot badan. Data yang diperoleh dianalisis menggunakan Analysis of Variance (ANOVA) pada taraf signifikansi 5% dan dilanjutkan dengan uji Duncan dengan signifikansi 5%. Hasil analisis data menunjukkan bahwa suplementasi daging ikan gabus dalam pakan memberi pengaruh nyata terhadap jumlah eritrosit dan bobot badan, namun tidak berpengaruh nyata terhadap kadar hemoglobin. Suplemen daging ikan gabus dalam pakan dengan konsentrasi 20% (P4) memberi pengaruh tertinggi terhadap jumlah eritrosit dan bobot badan, lebih tinggi dibanding perlakuan lainnya (5%, 10%, 15%. Simpulan penelitian ini adalah, suplementasi daging ikan gabus dalam pakan memberi pengaruh terhadap jumlah eritrosit dan bobot badan namun tidak berpengaruh terhadap kadar hemoglobin pada hewan uji yang diberi stres.
SUPLEMENTASI TEPUNG KULIT KAYU MANIS DAN DAUN PEGAGAN DALAM PAKAN TERHADAP KANDUNGAN KOLESTEROL DAN ANTIOKSIDAN TELUR PUYUH ( Sunarno sunarno; Muhammad Anwar Djaelani
Bioma : Jurnal Ilmiah Biologi Vol 7, No 1 (2018): Bioma
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v7i1.2539

Abstract

ABSTRACTCholesterol in eggs become constraints limiting consumption eggs by the part of community. The purpose of this study was to find supplement formula in cinnamon bark and leaf pegagan to produce low quail eggs cholesterol and rich in antioxidants. The method used in this study was a complete randomized design consisting of eight treatments with three replications. Eight such treatments, including controls, standard feeds supplemented with cinnamon bark flour 5% and 10%, standard feeds supplemented with pegagan leaf flour 5% and 10%, standard feeds supplemented with flour from cinnamon bark and pegagan leaf (5%:5%, 5%:10% and 10%:5%). The results showed that supplementation of flour from cinnamon bark and pegagan leaf in the feed can increase body weight, egg weight and antioxidant content of egg yolk but decrease the amount or productivity of egg and yolk cholesterol content of quail eggs. Supplementation of flour from cinnamon bark in the feed with concentration 5% or a combination of flour from cinnamon bark-pegagan leaf with ratio 5%:10% gived the best influence on some parameters observed. Low cholesterol and rich in antioxidants can provide solutions to meet the needs of animal protein and improve the quality of public health. Keywords: antioxsidants, cholesterol, cinnamon bark, pegagan leaf, quail eggs  ABSTRAKKolesterol dalam telur burung puyuh menjadi kendala pembatasan konsumsi masyarakat terhadap telur puyuh. Tujuan penelitian ini mencari formula suplemen dalam pakan berbahan kulit kayu manis dan daun pegagan dan menghasilkan telur puyuh rendah kolesterol dan kaya antioksidan. Metode yang digunakan dalam penelitian ini adalah rancangan acak lengkap yang terdiri atas 8 perlakuan dengan 3x ulangan. Delapan perlakuan tersebut, meliputi kontrol, pakan yang diberi suplemen tepung kulit kayu manis 5% atau 10%, pakan yang diberi suplemen tepung daun pegagan 5% atau 10%, pakan yang diberi suplemen tepung kulit kayu manis dan pegagan (5%:5%, 5%:10% atau 10%:5%). Hasil penelitian menunjukkan bahwa suplemen tepung kulit kayu manis dan daun pegagan dalam pakan dapat meningkatkan bobot tubuh, bobot telur, dan kadar antioksidan kuning telur namun menurunkan jumlah/produktivitas telur dan kadar kolesterol kuning telur pada puyuh. Suplemen tepung kulit kayu manis dalam pakan dengan konsentrasi 5% atau kombinasi tepung kulit kayu manis-daun pegagan dengan rasio 5%:10% memberi pengaruh terbaik terhadap beberapa parameter yang diamati. Telur puyuh rendah kolesterol dan kaya antioksidan dapat memberi solusi terhadap pemenuhan kebutuhan protein hewani dan peningkatan kualitas kesehatan masyarakat. Kata kunci: antioksidan, kolesterol, kayu manis, pegagan, telur puyuh
Bobot otot dan tulang tibia itik pengging (Anas platyrhyncos domesticus L.) setelah pemberian imbuhan tepung daun kelor (Moringa oleifera Lam.) dalam pakan Hikmah Putri Pratiwi; Kasiyati Kasiyati; Sunarno Sunarno; Muhammad Anwar Djaelani
Jurnal Biologi Tropika Vol. 2, No. 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.952 KB) | DOI: 10.14710/jbt.2.2.54-61

Abstract

Moringa leaf contains the nutrient which includes carbohydrates, protein, fat, fiber, beta-N, calcium, and phosphor. Various kinds of nutrient are essential supporting the growth and productivity of Pengging ducks, both egg or meat production. The aimed of this study to analyze the effect of Moringa leaves powder in feed on the weight of tibial bone and muscle as well as the length and diameter of tibial bone in Pengging ducks. The study used a Completely Randomized Design (CRD) consisting of 5 treatments with 3 replications. The treatment included the control group (basal feed without the addition of Moringa leaves powder), the treatment of basal feed supplemented with Moringa leaves powder by 2.5; 5; 7.5; and 10%. The data wereanalyzed by one way-ANOVA and the relationship between variables can be seen from the value of the correlation coefficient. The results showed that the added Moringa leaves powder did not significantly (P > 0.05) affect the weight of the tibial bone and muscle, and the lenght and diameter of the tibial of the pengging duck. The positive correlation between the length of thetibial bone and the diameter of the tibial bone with an r-value by 0.894. It was concluded that Moringa leaves powder as feed additive of Pengging duck could not increase the weight of the tibial bone and muscle, and the length and diameter of the tibial bone had not changed.
Bobot telur, indeks bentuk telur, dan nilai kantung udara telur itik pengging setelah pemberian imbuhan tepung daun kelor (Moringa oleifera Lam.) dalam pakan Novia Marcelina; Muhammad Anwar Djaelani; Sunarno Sunarno; Kasiyati Kasiyati
Jurnal Biologi Tropika Vol. 3, No. 1, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.566 KB) | DOI: 10.14710/jbt.1.1.1-7

Abstract

Moringa oleifera leaves contain nutrition of protein, lipid, carbohydrate, fiber, calcium, phosphorus as well as bioactive content such as tannin and saponin, that have the potential to improve egg quality. The objective of this research to evaluate the effect of Moringa powder of weight, shape index, and air space value of Pengging duck eggs. The study used a completely randomized design (CRD) consisting of 5 treatments with four replication, i.e., K0 (100% basal feed without Moringa leaves powder), K1 (basal feed and 2.5% Moringa leaves powder), K2 (basal feed and Moringa leaves powder), K3 (basal feed and 7.5% Moringa leaves powder) and K4 (basal feed and 10% Moringa leaves powder). The data were obtained  from a total of 60 female Pengging duck of 28 weeks old or sexually mature. Feed treatments administrated for six weeks started in 26 to 32 weeks old ducks. The measurement of research variables was carried out every week and measured for three weeks. The data of the study were analyzed by one way-ANOVA at a significant difference level of  5%. The results showed that the addition of Moringa leaves powder did not a significant difference (P > 0.05) in weight, shape index, and air space value of pengging duck eggs. Overall concluded that the addition of Moringa leaves powder with a concentration of 2.5; 5; 7.5; dan 10% in Pengging duck feed could not change egg quality.
Bobot Karkas dan Bagian Karkas Ayam Petelur Jantan (Gallus gallus domesticus L.) Setelah Pemberian Tepung Daun Kelor (Moringa oleifera Lam.) Sebagai Imbuhan Pakan Dwi Rahmawati; Muhammad Anwar Djaelani; Kasiyati Kasiyati; Sunarno Sunarno
Jurnal Biologi Tropika Vol. 3, No. 2, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbt.3.2.65-72

Abstract

Consumption of meat as a source of animal protein has increased every year, especially the consumption of rooster. The increasing cost of poultry feed has made it difficult for breeders to produce roosters that have meat with high protein content. The cost of poultry feed can be minimized by the addition of herbal ingredients, one of which is the addition of leaf powder. Moringa oleifera leaf powder contains various kinds of amino acids and antioxidants that can be used as a supplement to additives feed. This study aimed to evaluate the effect of M. oleifera leaf powder on carcass weight rooster (Gallus gallus domesticus L.). Completely randomized design (CRD) used in this study consisted of 5 treatment groups with three replications, namely the control treatment group and the basal feed treatment group supplemented with Moringa leaf powder 1; 2; 3; and 4%. The feed used is the standard starter and finisher period rations formulated with Moringa leaf powder according to their respective concentrations. Parameters were measured during the seventh week when the rooster was 49 days old, which included: body weight, carcass weight, breast weight, and thigh weight. Based on the research conducted, it was found that giving Moringa leaf powder 1; 2; 3; and 4% as feed additives in rooster had no significant effect (p> 0.05) on body weight, carcass weight, breast weight, and thigh weight. Overall, the conclusion of this study is the addition of Moringa leaf powder with a concentration of 1; 2; 3; and 4% in rooster feed up to 7 weeks of age did not affect on carcass weight, breast weight, and thigh weight of rooster.
Pengaruh imbuhan tepung daun kelor (Moringa oleifera Lam.) dalam pakan pada kandungan protein dan kolesterol telur itik pengging (Anas platyrhyncos domesticus L.) Khunfaya Firnanda Putri Rossida; Sunarno Sunarno; Kasiyati Kasiyati; Muhammad Anwar Djaelani
Jurnal Biologi Tropika Vol. 2, No. 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.522 KB) | DOI: 10.14710/jbt.2.2.41-47

Abstract

One important component contained in Moringa leaves is protein. Apart from protein, bioactive flavonoids and saponins in Moringa leaves have potential as antioxidants and hypocholesterolemia. The objective of this study was to analyze the effect of Moringa leaf flour as a feed additive to protein content and cholesterol of Pengging duck eggs. The study design used was a Completely Randomized Design (CRD) with 5 treatments, and each treatment consists of 3 replications. The treatment group consisted of a control group and a basal feed treatment that was added with Moringa leaf flour by 2.5; 5; 7.5; and 10%. Measurement of duck egg protein content using the Bradford method and cholesterol measured by the Indiko method. Data were analyzed with one-way ANOVA. The addition of Moringa leaf flour on feed did not have a significant effect (P > 0.05) on the protein content and cholesterol of the Pengging duck eggs. The percentage of white and egg yolk weights did not increase significantly. The conclusion of this study was the use of Moringa leaf flour as a feed additive with a concentration of 2.5 to10% could not change the protein content and cholesterol of the Pengging duck eggs.
Co-Authors A. P. Nagari Adhitya Yoga Pradana Riyadi Ahmad Edi Darmawan Ahmad Rifai Alfisa Devi Amni Z. Rahman Annisa Widyasari Bayu Pradika Beta Cahyaning Nugraheni Bima Adistya Dara Puspita Jati Windoro Dwi Rahmawati Eko Purnomo Endang Nawarikita Evi Lestari F Pratiwi Falasifah Falasifah Fendy E. Wahyudi Garin F Fairuzzaki Hikmah Putri Pratiwi K. Budiraharjo Kasiyati Kasiati Kencana Ayudya Prabahandari Khunfaya Firnanda Putri Rossida Khunfaya Firnanda Putri Rossida Laili Fitria Zulfa Linda Ayu Mamluatul Lailiyah Maulida Aqlinia Mohammad Farhan Aditya Muhammad Adnan Jafar Alfian Muhammad Alvin Gibran Muhammad Anwar Djaelani Muhammad Fikri Muhammad Fikri Zulfikar Novia Marcelina Rahayu Damayanti Rd Yudhi Tresnadi Kusumah Rully Rahadian Salindri Prawitasari Salsabila Alifah Salsabila Alifah Satwika Paramasatya, Satwika Silvana Tana Siska Hairunnisa Siti Muflichatun Mardiati Siti Murni Mas&#039;adah Siti Murni Mas&#039;adah Solikhin Solikhin SRI ISDADIYANTO Sri Pujiyanto Sri Widodo Agung Suedy Susan Nuraeni Vinsensius M Dhani Widiartanto Widiartanto