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JAMUR ENDOFITIK YANG DIISOLASI DARI BUNGA ANDROGRAPHIS PANICULATA (SAMBILOTO) SEBAGAI SUMBER SENYAWA ANTIBAKTERI Rani Aulia Suhanah; Suryelita Suryelita; Sri Benti Etika; Mariam Ulfah; Riga Riga
Jurnal Insan Farmasi Indonesia Vol 4 No 1 (2021): Jurnal Insan Farmasi Indonesia
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jifi.v4i1.664

Abstract

Tumbuhan Andrographis paniculata (Sambiloto) memproduksi beragam metabolit sekunder dengan berbagai aktivitas biologi, termasuk antibakteri. Selain dari tumbuhan alami, potensi senyawa antibakteri dari tumbuhan A. paniculata dapat dieksplorasi dari jamur endofitiknya. Tujuan dari penelitian ini adalah melakukan kajian terkait potensi jamur endofitik yang berkolonisasi dalam jaringan bunga tumbuhan A. paniculata. Secara garis besar penelitian ini terdiri dari isolasi, kultivasi, ekstraksi, uji antibakteri dan uji kandungan metabolit sekunder dari jamur endofitik pada bunga tumbuhan A. paniculata. Hasil uji aktivitas antibakteri terhadap ekstrak EtOAc jamur BS-1 yang diisolasi dari bunga tumbuhan A. paniculata menunjukkan kemampuannya dalam menginhibisi pertumbuhan semua bakteri uji pada konsentrasi 1%, 3%, dan 5%. Studi terkait senyawa yang berkontribusi dalam sifat antibakteri ini perlu dilakukan lebih lanjut. Untuk infomasi bagi kita semua bahwa penelitian terkait kajian sifat antibakteri dari jamur yang hidup pada bunga A. paniculata baru pertama kali dilakukan pada kesempatan ini.
Improvement of MGMP Chemistry Teacher Competency in 50-Kota Districts on OSN Preparing Students Edi Nasra; Sri Benti Etika; Desy Kurniawaty; Bahrizal Bahrizal; Eka Yusmaita
Pelita Eksakta Vol 2 No 2 (2019): Pelita Eksakta Vol. 2 No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol2-iss2/46

Abstract

Training in increasing the competence of MGMP Chemistry teachers in 50 City Regencies in preparing students for the National Science Olympiad (OSN). The activity began with filling out a questionnaire for the readiness of the teachers in the learning process and pre-test to see the initial abilities of the MGMP Chemistry teachers in the City 50 District. After training in strengthening chemical materials and discussing HOTS questions in the fields of Analytical Chemistry, Organic Chemistry, Inorganic Chemistry, Physical Chemistry and Biochemistry, a post test was conducted to evaluate the achievement of the objectives of the activity. From the questionnaire given at the beginning of the activity it can be concluded that in general the teachers were ready for learning even though they were not used to the HOTS questions of the Olympic type questions. This is directly proportional to the pre-test results with an average value of 3.625 (scale 10). After the training of the teachers there was a significant increase which was shown by the results of the post test with an average of 5.5 (scale 10). As an evaluation material, at the end of the activity the satisfaction questionnaire was distributed and the results obtained were an average of a questionnaire of 3.47 (scale 4). Generally teachers complain about the lack of training time so that the absorption of the material provided is quite low.
PKM Utilization of Natural Ingredients, Spices and Coconut as Family Drinks in an Effort to Increase Body Immune During Covid Conditions in Bungus Teluk Kabung District, Bungus Barat Village, Padang City Suryelita Suryelita; Syamsi Aini; Desy Kurniawati; Indang Dewata; Sri Benti Etika; Melindra Mulia; Trisna Kumala Sari
Pelita Eksakta Vol 5 No 1 (2022): Pelita Eksakta Vol. 5 No. 1
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol5-iss1/163

Abstract

During the pandemic, people's activities are limited outside the home. However, this condition has an impact on their income economy. People can return to their activities to meet their needs by implementing a new normal life through health protocols. However, information and knowledge on how to maintain body immunity and the implementation of a new normal life are still limited. Therefore, it is necessary to disseminate information about the importance of body immunity during this pandemic. One of the areas affected by this pandemic is Bungus Barat Village, Bungus Teluk Kabung District. To answer the problems that arise in this area, it is necessary to carry out service activities that aim to provide information to the community, especially mothers who are the spearheads of families who are members of the association of PKK women in West Bungus Village on how to increase and maintain immunity during the pandemic. This increase in immunity includes lifestyle and diet that must be followed in the new normal life. Participants in this service activity include representatives of PKK women in West Bungus Village. The activity is in the form of implementation which is divided into two stages, namely guided activities, and independent activities. In the guided activities, interactive and practical counseling methods are used. Meanwhile, in independent activities, the target is to carry out the practice of making spice drinks and VCO in groups again. The results of the activity showed an increase in target knowledge about kitchen spices and coconut into VCO and nutritious spice drinks. The target community, namely PKK women, is also interested in consuming and producing their own VCO and spice drinks that have been made. In addition, from this activity, the community gained a good understanding of what to do during this pandemic, not only in complying with health protocols but also in regulating lifestyle and eating patterns.
The Increasing The Competency of Chemical Teachers Of South Coast Regency To Prepare Students At The National Science Olimpiade Sri Benti Etika
Pelita Eksakta Vol 3 No 2 (2020): Pelita Eksakta Vol. 3 No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol3-iss2/113

Abstract

Training activities have been carried out to improve the competence of chemistry MGMP teachers in Pesisir Selatan district in preparing students to take part in Nasional Science Olimpiade (NSO) . So far, it is known that very few students qualify for NSO coming from the South Coastal district. One of the obstacles faced by schools in Pesisir Selatan district is the lack of adequate resources because problems in NSO require in-depth mastery of the material. Based on the results of the analysis of pretest and posttest in general the value of MGMP Chemistry teachers in Kab. Pesisir Selatan in general increased with an average value of 38.1. Although it did not increase significantly, this training was considered to have a positive impact on teachers with an average evaluation score of the activity of 3.48.
ISOLASI DAN KARAKTERISASI SENYAWA STEROID DARI DAUN CEMARA NATAL (Cupressus funebris Endl.) Suryelita Suryelita; Sri Benti Etika; Nivi Suci Kurnia
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 18 No. 01 (2017): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.1 KB) | DOI: 10.24036/eksakta/vol18-iss01/23

Abstract

Isolasi dan Karakterisasi Flavonoid dari Beras Ketan Hitam (Oryza Sativa L. Var Glutinosa) Sri Benti Etika; Iryani Iryani
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 20 No. 2 (2019): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (774.254 KB) | DOI: 10.24036/eksakta/vol20-iss2/186

Abstract

This study aims to isolate and characterize the isolated flavonoid compounds from glutinous rice (Oryza Sativa L. Var Glutinosa). The method that used was maserasi with methanol solvent, multilevel fractionation with n-hexane and ethyl acetate. The separation of chemical components using column chromatography and tested its purity by melting point test and TLC. Characterization of isolated flavonoids using color reagents (H2SO4, NaOH, Mg-HCl), KKt-2A, UV-Vis and FT-IR. The powder (amorphous) flavonoid isolation results have a melting point of 236.5-237.8 oC. Flavonoid powder added H2SO4 produces a yellow color, with NaOH producing a violet color, and Mg-HCl produces a red color. KKt-2A with a BAA developer has Rf 0.49 and 15% acetic acid having Rf 0.09. The UV-Vis spectrum shows maximum absorption at 532 nm wavelength. The results of powder analysis (amorphous) flavonoid using FT-IR showed absorption at wave number 3.330,40 cm-1, 2838,96 cm-1, 1,656,23 cm-1, 1,421,85 cm-1, 1,112,25 cm-1, 1,019,27 cm-1 and 697,76 cm-1. According to those data it can be concluded that flavonoids that isolated have the form of powder (amorphous) which belong to anthocyanin group that have o-diOH on ring B and have a conjugated double bond.Keywords- Black Sticky Rice, Flavonoid, Fractionation, FT-IR, Maserasi, UV-Vis.
A Preliminary Screening of the Different of Secondary Metabolites Ruku-Ruku Leaves (Ocimum tenuiflorum Linnen) in West Sumatera Hesty Parbuntari; Sri Benti Etika; Melindra Mulia; Elfanny Delvia
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 20 No. 2 (2019): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (813.011 KB) | DOI: 10.24036/eksakta/vol20-iss2/193

Abstract

Ruku-ruku leaves are used to remove distinctive odors from fish in some cuisine of West Sumatra. The characteristic odor comes from secondary metabolites especially essential oils. Therefore, secondary metabolites in ruku-ruku leaves have the potential to be further investigated as a source of bioactive compounds. The phytochemical screening of ruku-ruku leaves is a preminilary qualitative test that needs to be done to maximise its function as a medicinal plant. Based on the result data, there are some different result of flavonoid, steroids, and triterpenoids identification. Ruku-ruku leaves from Pesisir Selatan and Padang Panjang shows less flavonoid compounds tham Payakumbuh, Batusangkar, and Lubuk Alung. Both of this city and Payakumbuh also gives less steroids and triterpenoids. The factor influencing this result is caused by several factors, namely temperature, humidity, light exposure, and salinity.
Study of The Antibacterial Activity of Endophytic Fungus That Colonize With The Twig of Andrographis paniculata Varel Anshar Al Khairi; Sri Benti Etika; Suryelita Suryelita; Mariam Ulfah; Riga Riga
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 22 No. 2 (2021): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1063.338 KB) | DOI: 10.24036/eksakta/vol22-iss2/266

Abstract

Reports of the chemical constituents of Andrographis paniculata showed that this plant produced various secondary metabolites with antibacterial activity. Further exploration of bioactive compounds from A. paniculata can also be conducted by analyzing its endophytic fungi. Isolation of endophytic fungi from the twig of A. paniculata obtained three isolates of endophytic fungi. One of the isolates, RS-2, was fermented on rice media and extracted with ethyl acetate to give the EtOAc extract. The EtOAc extract from fungus RS-2 was analyzed for their antibacterial and phytochemical screening. The results exhibiting the EtOAc extract of fungus RS-2 has activity to inhibite bacterial growth. Overall, the study of the antibacterial activity of endophytic fungus obtained from the twig of A. paniculata was firstly carried out in this study
Characterization of Biodegradable Plastic Nata De Soya Using Glycerol and Palm Oil Addictive Substances Iryani Iryani; Iswendi Iswendi; Sri Benti Etika; Citra Devira; Regi Fadila Putra
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 22 No. 3 (2021): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (821.117 KB) | DOI: 10.24036/eksakta/vol22-iss3/285

Abstract

This research aimed to analyze the characterization of biodegradable plastic nata de soya (NDS). The addictive substance used to manufacture this plastic is a mixture of glycerol and palm oil with a saturated solution of arabic gum as an emulsifier. The volume variations are A (10:5:15) mL, B (10:10:30) mL, C (10:15:45) mL, control is (10 mL of 3% glycerol) and soaking time for NDS sheets with additives are 4, 6, and 8 days. The results showed that the highest degradation power of NDS is 87% in the variation of additive C with an immersion period of 8 days had a degree of crystallinity of 8.075%. The water resistance test was 318% in an immersion of NDS plastic with a variation of C for four days had a degree of crystallinity of 12.47%. The highest tensile strength value of plastic was 23.459 MPa in the composition of additive C with four days of immersion and had a degree of crystallinity of 13.172%.
KAJIAN FITOKIMIA DAN SIFAT ANTI BAKTERI JAMUR ENDOFITIK RS-1 PADA RANTING ANDROGRAPHIS PANICULATA (SAMBILOTO) DENGAN MEDIA PERTUMBUHAN BERAS MERAH Melisa Yolanda; Sri Benti Etika; Riga Riga
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 7 No 1 (2022): Juli
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v7i1.3924

Abstract

Andrographis paniculata (Sambiloto) was reported to produce secondary metabolites with various biological activities including antibacterial. The antibacterial compounds from sambiloto can also be explored from other sources. One of them is their endophytic fungi. The purpose of this research was to determine the antibacterial activity of endophytic fungi cultivated in red rice media. The steps of this research were isolation, cultivation, extraction, antibacterial test and test the content of secondary metabolites from endophytic fungi derived from the twigs of A. paniculata. An endophytic fungus labelled with RS-1 has been isolated from the twigs of A. paniculata. The results of the antibacterial activity test showed that the ethyl acetate extract of the fungus RS-1 had the ability to inhibit the growth of all tested bacteria at concentrations of 1%, 3%, and 5%. The ethyl acetate extract of the endophytic fungus RS-1 isolated from the A. paniculata has potential to produce antibacterial compound.