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All Journal Jurnal Farmasi Udayana Edumatsains Jurnal Zarah Jurnal Penelitian Farmasi Indonesia Jurnal Sains Natural Eksakta : Berkala Ilmiah Bidang MIPA Pelita Eksakta SPIN: Jurnal Kimia & Pendidikan Kimia Indonesian Journal of Chemical Science and Technology Jurnal Insan Farmasi Indonesia CHEDS: Journal of Chemistry, Education, and Science Jurnal Sains dan Kesehatan Jurnal Pengabdian Pada Masyarakat Parapemikir Jurnal Ilmiah Farmasi

Riga Riga

Universitas Negeri Padang

Author-ID : 2578038

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JAMUR ENDOFITIK YANG DIISOLASI DARI BUNGA ANDROGRAPHIS PANICULATA (SAMBILOTO) SEBAGAI SUMBER SENYAWA ANTIBAKTERI Rani Aulia Suhanah; Suryelita Suryelita; Sri Benti Etika; Mariam Ulfah; Riga Riga
Jurnal Insan Farmasi Indonesia Vol 4 No 1 (2021): Jurnal Insan Farmasi Indonesia
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jifi.v4i1.664

Tumbuhan Andrographis paniculata (Sambiloto) memproduksi beragam metabolit sekunder dengan berbagai aktivitas biologi, termasuk antibakteri. Selain dari tumbuhan alami, potensi senyawa antibakteri dari tumbuhan A. paniculata dapat dieksplorasi dari jamur endofitiknya. Tujuan dari penelitian ini adalah melakukan kajian terkait potensi jamur endofitik yang berkolonisasi dalam jaringan bunga tumbuhan A. paniculata. Secara garis besar penelitian ini terdiri dari isolasi, kultivasi, ekstraksi, uji antibakteri dan uji kandungan metabolit sekunder dari jamur endofitik pada bunga tumbuhan A. paniculata. Hasil uji aktivitas antibakteri terhadap ekstrak EtOAc jamur BS-1 yang diisolasi dari bunga tumbuhan A. paniculata menunjukkan kemampuannya dalam menginhibisi pertumbuhan semua bakteri uji pada konsentrasi 1%, 3%, dan 5%. Studi terkait senyawa yang berkontribusi dalam sifat antibakteri ini perlu dilakukan lebih lanjut. Untuk infomasi bagi kita semua bahwa penelitian terkait kajian sifat antibakteri dari jamur yang hidup pada bunga A. paniculata baru pertama kali dilakukan pada kesempatan ini.

Counseling on The Technology of Processing Quail Feces Waste into Biogas in Mungka District, Lima Puluh Kota Regency Trisna Kumala Sari; Syamsi Aini; Edi Nasra; Riga Riga; Edwina Zainal
Pelita Eksakta Vol 4 No 2 (2021): Pelita Eksakta Vol. 4 No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol4-iss2/161

The people of Jorong Padang Koto Tuo, Mungka District, Lima Puluh Kota Regency are increasingly enthusiastic about quail farming. The more developed the livestock business, the more manure is produced. This will certainly become a serious environmental problem, if not immediately followed up. Therefore, it is necessary to take action to treat this manure, including the use of appropriate technology for processing quail manure into biogas and fertilizer. This activity focused on educating the people of Jorong Padang Koto Tuo, Mungka District, Lima Puluh Kota Regency regarding the processing of quail waste into biogas as an alternative energy source that is environmentally friendly and inexpensive, starting from the tools, how the stages of work, what processes occur and the products produced. This activity not only has a good impact on the environment, but the resulting product can be used as a substitute for LPG gas. The people looks enthusiastic in participating in this activity.

A Training of Organic Fertilizer Preparation from Wasted Rice Straw in Rice Caltivation Area-Nagari Dilam, Bukit Sundi District, Solok Regency Okta Suryani
Pelita Eksakta Vol 5 No 1 (2022): Pelita Eksakta Vol. 5 No. 1
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol5-iss1/178

Abstract – Rice is one of the national mainstay commodities that have an important role in the Indonesian economy, which is also a mainstay crop commodity in Solok Regency. Solok Regency is famous for its high quality rice. Nagari Dilam, Bukit Sundi District, as a village with a large rice commodity in Solok Regency, most of the residents have a livelihood as rice farmers. To obtain good yields, the material that is needed during the planting process is fertilizer. However, fertilizer problems almost always appear every year in this area. In the last 2 years, farmers have complained due to these problems, including the price of commercial inorganic fertilizers (Urea, SP36, KCl, ZA) which tends to increase. In addition, without realizing it, the intensive use of commercial inorganic fertilizers can cause a decrease in soil fertility and soil organic matter decreases. The idea of using wasted straw came up as a way to solve these problem to prodice orgnic fertilizer. A training in preparation of organic fertilizer from wasted straw has been conducted in Nagari Dilam. The produced organic fertilizer has been characterized for N, P,K containing subtance. The fertilizer contains 0.030%, 4.563% and 1.369% of N, P, and K respectively. Keywords Organic, Fertilizer, Wasted Straw, Rice

Aktivitas Sitotoksik dan Antibakteri Ekstrak Etil Asetat Jamur Endofitik Colletotrichum gloeosporioides Riga Riga; Euis Holisotan Hakim
Jurnal Farmasi Udayana Vol. 10, No 2, Tahun 2021
Publisher : Departement of Pharmacy, Faculty of Mathematics and Natural Science, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JFU.2021.v10.i02.p15

Artocarpus heterophyllus merupakan salah satu tumbuhan yang dilaporkan menghasilkan beragam senyawa yang bersifat sitotoksik dan antibakteri. Selain melakukan studi kimia terhadap tumbuhan alaminya, eksplorasi terkait kajian fitokimia tumbuhan ini dapat dilakukan terhadap jamur endofitik yang berkolonisasi dengan tumbuhan A. heterophyllus. Tujuan penelitian ini adalah melakukan uji aktivitas antibakteri dan sitotoksik ekstrak EtOAC jamur endofitik yang berkolonisasi dengan daun A. heterophyllus. Tahapan dari penelitian ini terdiri dari inokulasi, sub-kultur, kultivasi jamur endofitik yang dilanjutkan dengan ekstraksi, uji aktivitas biologi dan uji kandungan kimia dari ekstrak etil asetat jamur Colletotrichum gloeosporioides yang diisolasi dari daun A. heterophyllus. Hasil uji sitotoksik menunjukkan bahwa ekstrak EtOAc jamur bersifat aktif terhadap sel murin leukemia P-388 dengan nilai IC50 18,97 µg/mL. Hasil uji antibakteri juga menunjukkan bahwa ekstrak EtOAc mempunyai kemampuan dalam menghambat pertumbuhan keempat bakteri uji dengan rentang zona hambat 4,3 – 14,3 mm. Untuk infomasi bagi kita semua bahwa penelitian terkait kajian sifat antibakteri dan sitotoksik dari jamur yang hidup pada daun A. heterophyllus baru pertama kali dilakukan pada kesempatan ini.

Pencil Lead Electrode Modified with Gold Thin Layer for Voltammetric Detection of Chromium (VI) Trisna Kumala Sari; Riga Riga; Moondra Zubir
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 22 No. 2 (2021): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Cr(VI) is a toxic, mutagenic, and carcinogenic metal. This heavy metal have effect harmful on organism and the environment. In this study, an electroanalytic approach was improved for detection of the Cr(VI) using a pencil lead electrode modified with gold thin layer by cyclic voltammetry. Gold thin layer was electrodeposited on the pencil lead electrode surface with potential-sweeping technique at scan of potential from 1.2 V to 0 V. Since the Cr(VI) species depends on the pH, effect of supporting electrolytes matrix at various pH were investigated. It was found that Cr(VI) gave a reduction peak with a peak potential of 0.35 V vs Ag/AgCl in cyclic voltammogram with 0.1M HClO4 as supporting electrolyte. The calibration curve for Cr(VI) at gold thin layer modified pencil lead electrode shows linearity in range of 5 µM to 100 µM with a detection limit of 2.3 µM achieved.

Study of The Antibacterial Activity of Endophytic Fungus That Colonize With The Twig of Andrographis paniculata Varel Anshar Al Khairi; Sri Benti Etika; Suryelita Suryelita; Mariam Ulfah; Riga Riga
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 22 No. 2 (2021): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Reports of the chemical constituents of Andrographis paniculata showed that this plant produced various secondary metabolites with antibacterial activity. Further exploration of bioactive compounds from A. paniculata can also be conducted by analyzing its endophytic fungi. Isolation of endophytic fungi from the twig of A. paniculata obtained three isolates of endophytic fungi. One of the isolates, RS-2, was fermented on rice media and extracted with ethyl acetate to give the EtOAc extract. The EtOAc extract from fungus RS-2 was analyzed for their antibacterial and phytochemical screening. The results exhibiting the EtOAc extract of fungus RS-2 has activity to inhibite bacterial growth. Overall, the study of the antibacterial activity of endophytic fungus obtained from the twig of A. paniculata was firstly carried out in this study

KAJIAN FITOKIMIA DAN SIFAT ANTI BAKTERI JAMUR ENDOFITIK RS-1 PADA RANTING ANDROGRAPHIS PANICULATA (SAMBILOTO) DENGAN MEDIA PERTUMBUHAN BERAS MERAH Melisa Yolanda; Sri Benti Etika; Riga Riga
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 7 No 1 (2022): Juli
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v7i1.3924

Andrographis paniculata (Sambiloto) was reported to produce secondary metabolites with various biological activities including antibacterial. The antibacterial compounds from sambiloto can also be explored from other sources. One of them is their endophytic fungi. The purpose of this research was to determine the antibacterial activity of endophytic fungi cultivated in red rice media. The steps of this research were isolation, cultivation, extraction, antibacterial test and test the content of secondary metabolites from endophytic fungi derived from the twigs of A. paniculata. An endophytic fungus labelled with RS-1 has been isolated from the twigs of A. paniculata. The results of the antibacterial activity test showed that the ethyl acetate extract of the fungus RS-1 had the ability to inhibit the growth of all tested bacteria at concentrations of 1%, 3%, and 5%. The ethyl acetate extract of the endophytic fungus RS-1 isolated from the A. paniculata has potential to produce antibacterial compound.

AKTIVITAS ANTIBAKTERI JAMUR ENDOFITIK RS-2 YANG DIISOLASI DARI TUMBUHAN SAMBILOTO (Andrographis paniculata) Riga Riga; Suryelita Suryelita; Sri Etika; Rani Aulia Suhanah; Varel Anshar Al Khairi
Jurnal Zarah Vol. 10 No. 1 (2022): Jurnal Zarah
Publisher : Jurnal Zarah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31629/zarah.v10i1.4229

Andrographis paniculata yang dikenal dengan tumbuhan sambiloto dilaporkan menghasilkan berbagai metabolit sekunder dengan aktivitas antibakteri. Potensi senyawa antibakteri dari tumbuhan ini dapat dikaji lebih lanjut dari jamur yang berasosiasi dengan tumbuhan sambiloto. Tujuan dari studi ini adalah menganalisis potensi jamur endofitik yang berasosiasi dengan ranting tumbuhan sambiloto. Hasil inokulasi terhadap tumbuhan sambiloto menghasilkan jamur endofitik dengan kode RS-2. Uji aktivitas antibakteri terhadap ekstrak jamur RS-2 mengindikasikan bahwa jamur RS-2 mampu menghambat pertumbuhan dua bakteri uji dengan konsentrasi 1%, 3%, dan 5%. Studi lebih lanjut terkait senyawa bioaktif yang berkontribusi dalam aktivitas antibakteri jamur RS-2 perlu dilanjutkan.

Antibacterial Activity and Phytochemical Screening of The Fraction of Endophytic Fungus Derived from Sambiloto Flowers (Andrographis paniculata (Burm.f.) Nees) Firda Febria; Suryelita Suryelita; Riga Riga
JURNAL SAINS NATURAL Vol. 12 No. 3 (2022): Sains Natural
Publisher : Universitas Nusa Bangsa

Sambiloto is one of the plants in Acanthaceae family obtaining antibacterial compounds. Another source to identify the antibacterial compounds from sambiloto is endophytic fungus. The study aimed to observe the antibacterial activity and phytochemical constituents of the fractions of endophytic fungus isolated from sambiloto flowers (Andrographis paniculata (Burm.f.) Nees). Endophytic fungus was extracted using ethyl acetate to give the crude extract. The crude extract was fractioned by Vacuum Liquid Chromatography (VLC) using hexane-ethyl acetate as the solvents to give 17 fractions. In addition, 7 fractions were created by combining the identical stain patterns on the chromatograms. The phytochemical composition of the isolate fraction has been tested, and it was proven that it contained terpenoid and steroid components. The antibacterial activity of the 7 fractions was also examined using the disc diffusion method with a concentration of 5%. The semi-polar fraction actively inhibits bacterial growth.Keywords:Â antibacterial;Â Andrographis paniculata;Â fractination;Â phytochemical;Â endophytic fungusÂ ABSTRAKAktivitas Antibakteri dan Kandungan Fitokimia dari Fraksi Jamur Endofitik pada Bunga Sambiloto (Andrographis paniculata (Burm.f.) Nees)Sambiloto adalah salah satu tanaman dalam famili Acanthaceae yang dilaporkan menghasilkan senyawa antibakteri. Sumber lain untuk mengidentifikasi senyawa antibakteri dari tumbuhan sambiloto adalah jamur endofitik. Tujuan dari penelitian ini adalah mengamati aktivitas antibakteri serta kandungan fitokimia dari fraksi jamur endofitik pada bunga sambiloto (Andrographis paniculata (Burm.f.) Nees). Jamur endofitik diekstraksi dengan etil asetat dan menghasilkan ekstrak pekat. Ekstrak pekat difraksinasi menggunakan metode Kromatografi Cair Vakum (KCV) menghasiilan 17 fraksi. Berdasarkan analisis noda pada kromatogram, fraksi tersebut digabungkan sehingga diperoleh tujuh fraksi. Ketujuh fraksi tersebut diuji kandungan fitokimianya yang menunjukkan positif mengandung senyawa terpenoid dan steroid. Ketujuh fraksi juga diuji aktivitas antibakteri menggunakan metode difusi cakram dengan konsentrasi ekstrak 5% . Fraksi yang bersifat semipolar lebih efektif menghambat pertumbuhan bakteri.Kata kunci: Andrographis paniculata;Â antibakteri; fitokimia; fraksinasi; jamur endofitik

A Training in Preparation of Organic Fertilizer from Wasted Rice Straw in Rice Valtivation Area-Nagari Dilam, Bukit Sundi District, Solok Regency Okta Suryani; Riga Riga
Pelita Eksakta Vol 5 No 2 (2022): Pelita Eksakta Vol. 5 No. 2
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol5-iss2/182

Rice is one of the national mainstay commodities that have an important role in the Indonesian economy, which is also a mainstay crop commodity in Solok Regency. Solok Regency is famous for its high-quality rice. Nagari Dilam, Bukit Sundi District, as a village with a large rice commodity in Solok Regency, most of the residents have a livelihood as rice farmers. To obtain good yields, the material that is needed during the planting process is fertilizer. However, fertilizer problems almost always appear every year in this area. In the last 2 years, farmers have been facing these two problems, including the price of commercial inorganic fertilizers (Urea, SP36, KCl, ZA) which tends to increase. In addition, without realizing it, the intensive use of commercial inorganic fertilizers can cause a decrease in soil fertility and soil organic matter decreases. The idea of using wasted straw came up as a way to solve all of mentioned problems to produce organic fertilizer. A training in preparation of organic fertilizer from wasted straw has been conducted in Nagari Dilam. The produced organic fertilizer has been analyzed for N, P,K containing substance. The fertilizer contains 0.030%, 4.563% and 1.369% of N, P, and K respectively.

Co-Authors Ahmad Raisan R.P Anes Vanesa Bali Yana Fitri DESY KURNIAWATI Desy Kurniawati Desy Kurniawati Dewi Meliati Agustini Dewi Meliati Agustini Dewi Meliati Agustini Dewi Meliati Agustini Dina Agustina Edi Nasra Edwina Zainal Euis Holisotan Hakim Eva Selviana Fajriah Azra Ferdi Henfi Pratama Firda Febria Iryani Iryani Isra Yeni Luqiyatur Rohmah M. Iqbal S.G MARIAM ULFAH ULFAH Mauline Adia Silvani Mauline Adia Silvani Melindra Mulia Melindra Mulia Melisa Yolanda Moondra Zubir Muhammad Habibul Ikhsan Muhammad Habibul Ikhsan Namira Putri Fadhillah Okta Suryani Rafigha Gusjelita Absa Rani Aulia Suhanah Rani Aulia Suhanah Rizka Purwanti Romy Dwipa Yamesa Away Salsabila Safitri Sari Purbaya Sri Benti Etika Sukma Amelia Suryelita . Syamsi Aini Syamsi Aini Tessa Faradilla Trisna Kumala Sari Varel Anshar Al Khairi Varel Anshar Al Khairi Wandi Oktria Wandi Oktria Yeni, Isra Zonalia Fitriza

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