Article Per Year (5 Year)
p-Index From 2019 - 2024
1.048
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Dasar Jurnal Kedokteran Brawijaya Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Journal of Agromedicine and Medical Sciences Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Syntax Literate : Jurnal Ilmiah Indonesia Indian Journal of Forensic Medicine & Toxicology
Dina Helianti
Department Of Histology, Faculty Of Medicine, University Of Jember, Jember, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Education Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Neuroscience Public Health

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search

 1 
Aktifitas Fisik Berat Menyebabkan Degenerasi Sel Hepatosit melalui Mekanisme Stres Oksidatif hairrudin, Hairrudin; Helianti, Dina; Widiastuti, Yulestrina
Jurnal Kedokteran Brawijaya Vol 27, No 2 (2012)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2012.027.02.2

Abstract

Hepar mempunyai peranan yang penting dalam mempertahankan homeostasis metabolisme tubuh. Stres oksidatif akibat aktifitas fisik berat dapat meningkatkan produksi Reactive Oxygen Species (ROS) yang kemudian menyebabkan kondisi patologis pada sel hepatosit, ditandai dengan perubahan struktur dan fungsinya. Penelitian ini bertujuan untuk mengetahui dampak stres oksidatif akibat aktifitas fisik berat terhadap kerusakan sel hepatosit. Penelitian ini menggunakan 16 ekor tikus putih Wistar jantan yang berumur 3 bulan, dengan berat badan antara 150 s.d. 200 gram yang dibagi ke dalam kelompok kontrol dan perlakuan. Aktifitas fisik berat yang berupa renang dengan beban dan intensitas maksimal diberikan pada kelompok kontrol, dilakukan setiap pagi selama 30 hari. Penilaian stres oksidatif ditentukan melalui pengukuran kadar MDA serum menggunakan teknik Thiobarbituric Acid Reactive Substance (TBARS). Perubahan histopatologi sel hepatosit berupa degenerasi sel dinilai berdasarkan klasifikasi sistem skoring NAFLD dari the Pathology Committee of the NASH Clinical Research Network. Rerata kadar MDA pada kelompok kontrol sebesar 42,38 nmol/ml, sedangkan pada kelompok perlakuan sebesar 89,63 nmol/ml. Pada pengamatan histopatologi hepar menunjukkan adanya proses degenerasi sel. Dapat disimpulkan bahwa aktifitas fisik berat dapat menyebabkan degenerasi sel hepatosit melalui mekanisme stres oksidatif.Kata Kunci: Aktifitas fisik berat, degenerasi, sel hepatosit, stres oksidatif.
Effect of Cacao on Plasma F2-Isoprostane Level, CD34 and ICAM-1 Expression of Coronary Arteries in Cigarette Smoking Exposed Rats Dina Helianti; Soetjipto; Widjiati; I Ketut Sudiana
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15729

Abstract

This research aimed to evaluate cardioprotective effects of cacao in smoking exposure condition that the effects were mediated through the anti-oxidant pathway by measure plasma F2-isoprostane level, Endothelial Progenitor Cell (EPC) enhancement by expression of CD34, while dysfunction endothelial condition was measured by expression of ICAM-1 in coronary arteries. This study subjected rats, divided into four groups: the normal control group (2 ml of aqua bidest, air exposure); the cigarette control group (2 ml of aqua bidest, cigarette smoke); cacao group 1 (1205 mg/kg BW/day, cigarette smoke); cocoa group 2 (2410 mg/kg BW/day, cigarette smoke). The oxidant biomarker, F2-isoprostane level was assessed using ELISA; CD34, and ICAM-1 expression in coronary arteries by immunohistochemistry. Cacao 1205 mg/kg BW/day significantly decreases plasma F2-isoprostane level, and ICAM-1 expression of coronary arteries in cigarette smoking exposed rat (p < 0.05) but there was not a significant increases CD34 (p < 0.05). Cocoa in cigarette smoke-exposed rats can prevent endothelial dysfunction through decrease F2-isoprostane but not increase CD34. The results of this study can be used as a basis for preventing endothelial dysfunction due to cigarette smoke by using cacao.
Shallot (Allium cepa L.) Skin Ethanol Extract Neutralizes Liver Oxidative Stress in Diazinon-Induced Wistar Rats Shahifa Audy Rahima; Rosita Dewi; Nindya Shinta Rumastika; Dina Helianti
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 6, No 1 (2022): Journal Qanun Medika Vol 6 No 01
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v6i1.8038

Abstract

ABSTRACTDiazinon has a hepatotoxic effect since it is metabolized in the liver involving Kupffer cells (KCs) activation, increasing reactive oxygen species (ROS). The flavonoids contained in shallot (Allium cepa L.) skin act as antioxidants neutralizing oxidative stress. This study aims to determine the effect of shallot skin ethanol extract (SSEE) on liver MDA level and activated KCs histopathology. The total flavonoids level of SSEE was measured using the aluminum chloride colorimetric method and resulted in 228.1 mg QE/g. Rats were divided into normal, diazinon, and SSEE groups. Diazinon was administered at a dose of 40 mg/kg b.w. for 7 days, followed by SSEE at the dose of 600, 900, and 1,200 mg/kg b.w. for 7 days. Liver malondialdehyde (MDA) level was measured using the MDA-TBA method. The results revealed that diazinon increased liver MDA level (p <0.05), while SSEE at doses of 900 and 1,200 mg/kg b.w. decreased liver MDA level equal to normal (p>0.05). Activated KCs in the SSEE group at a dose of 1,200 mg/kg b.w. was impressively equal to the normal group. In conclusion, SSEE at dose of 1,200 mg/kg b.w. neutralize liver oxidative stress due to diazinon indicated by the decrease of liver MDA level and activated KCs equal to normal.Keywords: antioxidant, diazinon, Kupffer cell, liver, MDA, shallotCorrespondence: rosita.fk@unej.ac.id
Increased Interleukin-4 in Farmers With Ascariasis in Rural Jember Adiz Dwiputra Rahmadhan; Yunita Armiyanti; Bagus Hermansyah; Dina Helianti; Yudha Nurdian
Jurnal ILMU DASAR Vol 22 No 1 (2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v22i1.15921

Abstract

Ascaris lumbricoides is nematode which often infest human in the world. The infestation of A. lumbricoides in the human will trigger the formation of Th2 immune responses through increased interleukin-4 released by innate immune cells. Th2 immune response generates B lymphocyte cells to produce IgE antibodies to eliminate A. lumbricoides. Therefore, IL-4 is a marker of Th2 immune response. The purpose of this study was to determine the prevalence of ascariasis and an increase of IL-4 in planters infested with A. lumbricoides as a marker of Th2 immune response. Our study was observational analytic with cross sectional design. 230 planters who were willing to be involved in the study gave stool samples for the diagnosis of ascariasis. 20 people who were positive for ascariasis and 20 people who were negative selected randomly for blood plasma samples. IL-4 levels in blood plasma were measured using ELISA. Differences in IL4 levels between positive and negative ascariasis were analyzed by the Mann Whitney statistical test with p value ≤ 0.05. The prevalence of ascariasis among planters in rural area of Jember was 16,52% which was included low prevalence. Univariate analysis showed that the mean and median levels of IL-4 ascariasis were greater than those of healthy planters. Mann Whitney test results showed that there were differences in levels of IL-4 between ascariasis and healthy planters (p≤0.05). Increased levels of IL-4 in planters with ascariasis indicate that A. lumbricoides infection triggers the formation of Th2 immune response as a mechanism for eliminating A. lumbricoides. Keywords: Interleukin-4, A. lumbricoides, Th2 immune response.
The Effect of Shallot Peel Ethanol Extract in Reducing Kidney MDA Levels in Diazinon-induced Wistar Rats Mukhammad Arif Hadi Khoiruddin; Dina Helianti; Muhammad Hasan; Rosita Dewi; Sheilla Rachmania
Journal of Agromedicine and Medical Sciences Vol 8 No 2 (2022)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v8i2.27424

Abstract

Diazinon is an organophosphate pesticide often used by farmer. The residue of diazinon may contaminate agricultural products and if accidentally consumed in a long term, may potentially led to health problems such as kidney disorders. Shallot (Allium cepa) peel was recently proposed to help recover the kidney damage due to its high flavonoid antioxidant content. This study aims to determine the antioxidant effect of shallot peel extract in reducing oxidative stress caused by diazinon with malondialdehyde (MDA) level indicator on the kidney of Wistar rats (Rattus norvegicus). This research was a true experimental with an in-vivo posttest-only control group design. The 28 rats were divided using a simple random sampling technique. The groups were the control group (K0), the diazinon group (K1), SPEE (Shallot Peel Ethanol Extract) groups which are P1 (300 mg/kg BW), P2 (600 mg/kg BW), P3 (900 mg/kg BW), P4 (1200 mg/kg BW) and P5 (2400 mg/kg BW). At the end of the study, the MDA kidney levels was analysed using the TBARS method. Effective doses were found in 600 mg/kg BW/day, 900 mg/kg BW/day, 1200 mg/kg BW/day, and 2400 mg/kg BW/day. The optimal dose of shallot peel extract in this study was 600 mg/kg BW/day. This study showed the effectiveness of shallot peel extract in reducing kidney MDA levels in diazinon-induced Wistar rats. Keywords: shallot peel, diazinon, kidney MDA level
EKSTRAK ETHANOL KULIT BAWANG MERAH (ALLIUM CEPA L.) MEMPERBAIKI KERUSAKAN HATI TIKUS WISTAR YANG DIINDUKSI DIAZINON Nilam Cahya; Rosita Dewi; Muhammad Hasan; Dina Helianti
JURNAL KEDOKTERAN DAN KESEHATAN Vol 9, No 3 (2022)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/JKK.V9I3.18862

Abstract

Diazinon merupakan pestisida organofosfat yang paling umum digunakan untuk mengendalikan hama. Konsumsi produk pertanian yang mengandung residu diazinon mengakibatkan akumulasi diazinon dan metabolitnya pada hati dan menimbulkan stress oksidatif. Kulit bawang merah (Allium cepa L.) mengandung flavonoid kuersetin yang dapat mengikat radikal bebas, menginduksi aktivitas enzim yang berperan penting dalam perbaikan sel, dan meningkatan jumlah dan kapasitas antioksidan endogen. Penelitian ini bertujuan mengetahui efek ekstrak ethanol kulit bawang merah (EKBM) dalam memperbaiki kerusakan hati tikus Wistar yang diinduksi diazinon. Tikus dibagi menjadi kelompok normal, diazinon, dan EKBM. Diazinon diberikan dengan dosis 40 mg/kgBB selama 7 hari dilanjutkan dengan EKBM dengan dosis 600, 900, dan 1200 mg/kgBB selama 7 hari. Pengamatan mikroskopik dilakukan terhadap 100 sel hati secara acak pada setiap preparat. Skor kerusakan sel hati ditentukan berdasarkan kriteria skor Manja Roenigk. Hasil penelitian menunjukkan bahwa diazinon meningkatkan skor kerusakan sel hati (p<0,05) dan EKBM dengan dosis 1200 mg/kgBB dapat menurunkan skor kerusakan sel hati sama dengan kelompok normal. Sebagai kesimpulan, EKBM dengan dosis 1200 mg/kg BB memperbaiki kerusakan hati tikus yang diinduksi diazinon yang ditunjukkan dengan penurunan skor kerusakan sel hati hingga tingkat normal.
Ekstrak Etanol Kulit Bawang Merah (Allium Cepa L.) Menurunkan Kadar Advanced Oxidation Protein Product (AOPP) Plasma Pada Tikus Wistar Terpapar Diazinon Sheilla Rachmania; Rosita Dewi; Dina Helianti; Ayu Munawaroh
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : CV. Ridwan Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (434.966 KB) | DOI: 10.36418/syntax-literate.v7i10.9837

Abstract

Penggunaan diazinon secara ekstensif menimbulkan dampak buruk terutama bagi pekerja di bidang agroindustri. Efek karsinogenik dan toksik diazinon pada organ terjadi melalui jalur stres oksidatif. Flavonoid kuersetin yang terkandung dalam kulit bawang merah (Allium cepa L.) dapat menetralisir kondisi stres oksidatif, terlebih turunan kuersetin, 2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxy-3(2H)-benzofuranone (BZF), mempunyai efek antioksidan jauh lebih tinggi dibandingkan kuersetin. Studi ini bertujuan mengetahui efek antioksidan ekstrak etanol kulit bawang merah (EKBM) terhadap stres oksidatif pada tikus Wistar terpapar diazinon melalui pengukuran kadar AOPP plasma dan memprediksi target protein BZF. Sebanyak 25 ekor tikus Wistar jantan dibagi dalam kelompok normal, kontrol, dan tiga kelompok perlakuan. Diazinon per oral diberikan dengan dosis 40 mg/kgBB pada 7 hari pertama, dilanjutkan EKBM dengan dosis 600, 900, dan 1200 mg/kgBB pada 7 hari berikutnya. Kadar AOPP plasma diukur dengan metode kolorimetri Witko-Sarsat. Analisis karakterisasi BZF dilakukan dengan pendekatan bioinformatika Swiss Target Prediction. Kadar AOPP pada ketiga kelompok perlakuan secara signifikan lebih rendah dibandingkan kelompok normal (p<0,05) dan antara dosis 600 mg/kg BB dan 900 mg/kgBB menunjukkan perbedaan tidak bermakna (p>0,05). Karakterisasi BZF menghasilkan prediksi target protein, yaitu pada Kanal Potasium Kv1.3 (skor probabilitas >0,65) yang berperan dalam proses inhibisi reaksi oksidasi protein. Sebagai kesimpulan, pada penelitian ini EKBM dengan dosis 600 mg/kgBB paling efektif untuk menetralisir stres oksidatif akibat paparan diazinon yang ditunjukkan dengan penurunan kadar AOPP plasma. BZF diprediksi memiliki target protein dengan probabilitas tertinggi, yaitu pada Kanal Potasium Kv1.3.
Co-Authors Adiz Dwiputra Rahmadhan Ayu Munawaroh Bagus Hermansyah Hairrudin Hairrudin I Ketut Sudiana Muhammad Hasan Muhammad Hasan Mukhammad Arif Hadi Khoiruddin Nilam Cahya Nindya Shinta Rumastika Rosita Dewi Rosita Dewi Rosita Dewi Rosita Dewi Shahifa Audy Rahima Sheilla Rachmania SOETJIPTO Widjiati Yudha Nurdian Yulestrina Widiastuti Yunita Armiyanti