Heri Setiyo Bekti
Department Of Medical Laboratory Technology, Health Polytechnic, Ministry Of Health Denpasar, Bali, Indonesia Center Of Excellence In Science And Technology, Health Polytechnic, Ministry Of Health Denpasar, Bali, Indonesia

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Journal : Muhammadiyah Medical Journal

Primers application with the Tso31 gene target in the molecular identification of Taenia solium Nur Habibah; Heri Setiyo Bekti; Ni Wayan Rika Kumara Dewi; Luh Putu Rinawati; Burhannuddin Burhannuddin; Aprilia Rakhmawati
Muhammadiyah Medical Journal Vol 2, No 1 (2021): Muhammadiyah Medical Journal (MMJ)
Publisher : Faculty of Medicine and Health Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.651 KB) | DOI: 10.24853/mmj.2.1.35-40

Abstract

Background: taeniasis is a zoonotic disease caused by Taenia spp. Human taeniasis caused by Taenia solium can be acquired after consumption of raw insufficiently cooked infected pork meat. Pigs are intermediate host for T.solium. Pigs acquired this infection by eating human feces that contained T.solium eggs. Pigs infected with T.solium can be transmitted to humans. Purposes: identification of T.solium in pig is important because it is indicator of T.solium transmission. Microscopic examination of T.solium eggs is considered less effective and efficient so that many other methods are developed for T.solium detection such as molecular and immunology. Method: This method used specific primer which can detect the Tso31 gene in T.solium. Tso31 gene is one of the most promising antigens to differentiate T.solium from T.saginata. Pig feces samples were taken by random sampling technique from 7 pig farms in Denpasar. Result: from the 30 samples, we found one sample that which gave a single amplification product of 234 bp. This indicates that the pig farms in Denpasar have been infected with T.solium. Conclusion: it is necessary to do meat inspection properly in the market as well as health education about the dangers and impacts of T.solium infection in the community.
The Effectiveness of Green Grape Extract (Vitis vinifera) on Decreasing White Rat (Rattus novergicus) Triglycerides Levels Ketut Lilik Arwati; Ni Nyoman Astika Dewi; Heri Setiyo Bekti; Ni Wayan Rika Kumara Dewi; Andi Udin Saransi
Muhammadiyah Medical Journal Vol 3, No 1 (2022): Muhammadiyah Medical Journal (MMJ)
Publisher : Faculty of Medicine and Health Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/mmj.3.1.1-9

Abstract

Background: Grapes (Vitis vinifera) are a source of antioxidants with high content of polyphenols and anthocyanins. Grape skin is rich in flavonoids. Anthocyanins and flavonoids have the effect of lowering blood triglyceride levels. Purposes: This study aimed to determine the effect of using green grape extract (GGE) in lowering triglyceride levels in the blood. Methods: Five groups of white male rats (Rattus novergicus) were divided into a negative control group (KN), positive control (KP), rats with a GGE dose of 100 mg/200g BW as P1, rats with a GGE dose of 250mg/200g BW as P2, and rats with GGE dose of 500 mg/200g BW as P3. Each group was given a hypercholesterolemic diet for five weeks. Groups P1, P2, and P3 were given GGE according to their respective doses for 14 days, then measured triglyceride levels in the blood. Results: The results showed that GGE 500mg/200g BW significantly reduced triglyceride levels in all treated mice. Conclusion: These results indicate that GGE has a great potential to treat dyslipidemia by lowering triglyceride levels in the blood.
Uric Acid Levels in Pregnant Women Anastaysia Annisa Haribaik; Heri Setiyo Bekti; Dewa Ayu Agustini Posmaningsih
Muhammadiyah Medical Journal Vol 3, No 2 (2022): Muhammadiyah Medical Journal (MMJ)
Publisher : Faculty of Medicine and Health Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/mmj.3.2.53-59

Abstract

Background: Preeclampsia in pregnant women can be detected by measuring uric acid levels in the blood. This can occur due to changes in the hemodynamic system and decreased glomerular filtration rate during pregnancy. Purposes: This study aims to determine the description of uric acid levels in pregnant women at Community Health Centers I, South Denpasar. Methods: This study uses a descriptive observational method from April to May 2022, with 31 respondents obtained by non-probability sampling technique. Uric acid levels were measured by the Point of Care Testing (POCT) method. Results: The results showed 23 pregnant women (74.2%) with normal uric acid levels and eight pregnant women (25.8%) with high uric acid levels. Conclusion: High uric acid levels are most suffered by pregnant women aged 30 years (16.1%), most commonly found in the third trimester of pregnancy (16.1%), most pregnant women with a body mass index (BMI) more and obesity (9.7%), and pregnant women with normal blood pressure (22.6%).