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Journal : Jurnal Biologi Tropis

Effects of Sargassum crassifolium Extract Formula as Biostimulant on Growth and Yield of Glycine max L. Merill Zozy Aneloi Noli; Muhammad Azwar
Jurnal Biologi Tropis Vol. 21 No. 3 (2021): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i3.2842

Abstract

The research about the Effect of Sargassum crassifolium Extract Formula as Biostimulant to Increase Growth and Yield of Glycine max L. Merill was conducted from February until June 2020 at the warehouse of Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Padang. The research aims to evaluate the effectiveness of liquid and powder extract formula of S. crassifolium as biostimulant on the growth and yield of soybean (Glycine max L. Merill). The research was conducted by an experimental method arrange in a Completely Randomized Design (CRD). The experiment consisted of 3 treatments. Each treatment was laid out with nine replications. The treatments tested were control, liquid extract (0.4%), powder extract (1.6 g/L). The results showed that the application of liquid extract gave a higher tendency towards the improvement of the root system, fresh and dry weight, chlorophyll content, and 100 seeds weight of soybean plant. While the powder extracts shown the best result on the total seed weight. Both of extract formula had the potential to be used to increase growth and yield of soybean.
Application of Centella asiatica extract as Biostimulant on Growth of Kale (Brassica oleracea var. acephala DC) Sherin Dien Salsabila; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): April-June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.4806

Abstract

Research on the effect of pegagan (Centella asiatica) extract as a biostimulant on the growth of kale plants (Brassica oleracea var. acephala DC) was conducted from April 2022 to August 2022 in the Greenhouse and Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang. This study aims to determine the effect of giving Pegagan extract as a biostimulant that can improve the quality of kale plant leaves and the concentration of Pegagan extract that can increase the growth of kale plants. The method used in this research was an experimental method with a Completely Randomized Design (CRD) with five treatments and five replicates. Treatments included the following amounts of Pegagan extract: A. (0 mg/l), B. (12.5 mg/l), C. (25 mg/l), D. (50 mg/l), and E. (100 mg/l). The results showed that several concentrations of Pegagan extract had different effects on chlorophyll a, chlorophyll b, and total chlorophyll of kale plants. However, it did not give different effects on plant growth parameters, namely plant height, number of leaves, root length, wet weight, and dry weight of kale plants. Based on the proximate analysis, the application of Pegagan extract has not been able to influence the quality of the kale plant's water, ash, fat, carbohydrate, and vitamin C. Still, it can affect kale plants' protein and crude fiber content.
Callus Induction of Dendrobium discolor Through The Thin Cell Layer (TCL) Technique Added with 2,4-Dichlorophenoxyaceticacid Iga Permata Hany; Zozy Aneloi Noli; M. Idris
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4b.5808

Abstract

Thin Cell Layer (TCL) is a plant propagation technique using thin explants (1-2 mm) through tissue culture. Callus induction is a crucial step in establishing plant regeneration. The type and concentration of growth regulators influence callus formation. 2.4 D is a growth regulator that is commonly used for callus induction in various plants. The research aims to determine the effectiveness of thin explants and 2,4-D in inducing Dendrobium discolor callus. The method used a Completely Randomized Design (CRD) with four treatments (1, 2, 3, and 4 mg/L). Adding 1, 2, and 3 mg/L 2,4-D can induce 100% calluses through the TCL explant. The callus has a friable to compact texture. The result showed that 2 mg/L 2,4-D was the best concentration for callus induction of D. discolor through TCL technique, indicating the greenish color, friable texture, and meristematic tissue.
Organoid Culture Applications: Mini Systematic Review Rilwan Efendi; Puti Khairunnajwa Amar; Resti Rahayu; Putra Santoso; Zozy Aneloi Noli; Rita Maliza
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5387

Abstract

Recent scientific developments in the stem cell field have made it possible to create complex organoids, or structures that resemble complete organs, in vitro. In the majority of these methods, stem cells produced from stem cells or tissue progenitors are allowed to self-organize into three-dimensional (3D) structures using culture systems in three dimensions. The purpose of this study is to ascertain the use of organoid culture in the area of health. This kind of study employs the literature review methodology. The Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) criteria are referenced throughout the stages of the literature review performed in this study. The four processes that make up this level are identification, screening, eligibility, and acceptance. These discoveries showing that human stem cells and patient-derived pluripotent stem cells can be used to generate organoids open up a wide range of opportunities for modeling and disease development, toxicological research and medication discovery, and the field of regenerative medicine. Here, we discuss some of the most significant recent advancements in 3D human organoid production as well as the field's historical advancements. Finally, we discuss present restrictions and provide illustrations of how organoid technology may be used in the healthcare industry.
Application of Portulaca oleracea L. Extract as a Biostimulant with Several Types of Solvents on The Growth of Kale (Brassica oleracea var. achepala) Cleopatra; Zozy Aneloi Noli; M. Idris; Chairul; Suwirmen; Solfiyeni
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5711

Abstract

Plant extracts are one biostimulant sources that have the potential to increase plant growth and yield. Portulaca contains secondary metabolites, macro and micronutrients, and amino acids, which have the potential as biostimulants. The research aims to determine the best type of solvent in the Portulaca extraction process as a biostimulant. The method used in this study was a Completely Randomized Design (CRD) with three treatments (aquadest, ethanol, and methanol) and nine replicates. The application of Portulaca extract extracted with several solvents significantly affected root length parameters of kale, with the most extended the root length of 15.80 cm. However, it did not have a significantly different effect on other parameters. Based on the proximate analysis, Portulaca extract extracted with ethanol solvent produces a protein content of 25.26%.