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Induksi Perakaran Tunas Tetrastigma rafflesiae Miq. Pada Media Murashige-Skoog Dengan Penambahan Beberapa Konsentrasi Indole-3-Butyric Acid (IBA) secara In Vitro Febriyanti, Erni; Suwirmen, -; Idris, M.
Jurnal Biologi Universitas Andalas Vol 2, No 2 (2013)
Publisher : Universitas Andalas

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Abstract

An experiment on in vitro root induction of Tetrastigma rafflesiae Miq. on Murashige-Skoog (MS) medium suplemented with several concentrations of Indole-3-Butyric Acid (IBA) had been done from February to April 2013. The aims of this experiment was to determine physiological responses of T. rafflesiae’s shoot to the IBA on half strength media of MS for root induction stages. The experiment used a completely randomized design in seven treatments and four replications. The treatment was the addition of IBA in several concentration (i.e. 0.0; 0.5; 1.0; 1.5; 2.0; 2.5 and 3.0 mg/l). The results showed 0.5 mg/l suplemented IBA was more effective and efficient in root induction of shoot then other concentrations. It influenced the average number of roots (12.5 root/plantlet), the average length of root (4.98 cm) and the morphological of plantlets. The result indicated that plantlet of T. rafflesiae generated in in vitro have a higher possibility of artificial culture and give light for future conservation of Rafflesia.Keywords : Tetrastigma rafflesiae Miq., root induction, Indole-3-Butyric-acid.
PENGARUH KONSENTRASI THIDIAZURON (TDZ) DAN ARANG AKTIF PADA SUB KULTUR TUNAS PISANG KEPOK HIJAU (MUSA PARADISIACA L.) Sari, Detty Intan; Suwirmen, Suwirmen; Nasir, Nasril
Natural Science: Journal of Science and Technology Vol 4, No 3 (2015): Volume 4 Number 3 (December 2015)
Publisher : Univ. Tadulako

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Abstract

Penelitian mengenai  ?Pengaruh Beberapa Konsentrasi Thidiazuron (TDZ) dan Arang Aktif pada Sub Kultur Tunas Pisang Kepok Hijau (Musa paradisiaca L.)? telah dilaksanakan pada bulan Agustus sampai November 2014 di Laboratorium Fisiologi Tumbuhan dan Kultur Jaringan, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Padang. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi Thidiazuron, arang aktif dan kombinasi keduanya terhadap pertumbuhan sub kultur tunas pisang kepok secara in vitro. Penelitian ini menggunakan metode eksperimen Rancangan Acak Lengkap (RAL) Faktorial yang terdiri dari dua faktor, faktor pertama adalah pemberian TDZ terdiri dari tiga taraf (0,00; 0,04; 0,09 mg/l) dan faktor kedua adalah pemberian arang aktif yang terdiri dari empat taraf (0,00; 1,00; 2,00; 3,00 g/l) sehingga terdapat 12 kombinasi perlakuan dan tiga kali ulangan. Hasil penelitian menunjukkan bahwa penambahan beberapa konsentrasi TDZ berpengaruh secara signifikan pada jumlah tunas, tidak pada persentase hidup eksplan, tinggi tunas, jumlah akar dan panjang akar. Penambahan beberapa konsentrasi arang aktif memberikan pengaruh yang signifikan pada jumlah dan panjang akar,tidak pada persentase jumlah eksplan, jumlah tunas dan tinggi tunas. Adapun interaksi antara beberapa konsentrasi TDZ dan arang aktif berpengaruh pada parameter panjang akar dengan kombinasi terbaik adalah pada perlakuan 0,04 mg/l TDZ dan 1,00 g/l arang aktif yaitu 6,03 cm
DISEMINASI TEKNOLOGI FORMULATED DIET ALTERNATIF DALAM UPAYA MENINGKATKAN PRODUKTIVITAS BUDIDAYA IKAN NILA INTENSIF Efrizal, Efrizal; Nurmiati, Nurmiati; Agustien, Anthoni; Syam, Zuhri; Suwirmen, Suwirmen; Zakaria, Indra Junadi; Rusnam, Rusnam; Deswati, Deswati
Jurnal Hilirisasi IPTEKS Vol 4 No 4 (2021)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v4i4.580

Abstract

Fish cultivators generally experience difficulties providing quality artificial feed (pellets) due to a lack of knowledge and skills in processing technology and making alternative feeds. Besides that, the costs incurred for feed procurement are relatively large, reaching 70 - 80% of the total production costs. However, the provision of feed is often an obstacle because, in addition to the increasingly high price, the quality of available feed does not always match the nutritional needs needed by Fish. The provision of quality feed for intensively cultivated tilapia dramatically affects the resulting growth and productivity. The higher the quality of the feed, the production will also increase. One of the technologies that can be applied to overcome this problem is the manufacture of alternative formulated diets using local raw materials. The method used in this service activity is the transfer of knowledge presented in the socialization activities in the form of 1) introduction to the types of alternative fish feed ingredients sourced from the area, (2) preparation of artificial tilapia feed formulations, (3) manufacturing technology artificial feed (pellets) and 4) monitoring and assistance. The socialization was carried out in the form of a lecture and was followed by a question and answer session. The results of farmer activities recognize the types of local feed that can be used as fish feed, know the preparation of feed formulations, and alternative artificial feed manufacturing technologies. The knowledge and skills possessed by fish cultivators are expected to overcome the difficulties of fish farmers in providing feed so that fish production and cultivation business increase.
Peningkatan Kandungan Alkaloid Kalus Mahkota Dewa (Phaleria macrocarpa [Scheff.]Boerl.) Dengan Pemberian Prekursor Triptofan pada Medium Murashige & skoog Wenny Rahma Gusni; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 4, No 1 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.1.%p.2015

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An experiment aimed to increase alkaloid content of Phaleria macrocarpa callus using tryptophan as a precursor in Murashige & Skoog media was done from September 2013 to January 2014 at the Laboratory of Plant Physiology, Faculty of Sciences, Andalas University. This experiment used Completely Randomized Design (CRD) with 6 treatments and 4 replications. The treatments were 6 consentrations of tryptophan i.e.0 mg/L (control), 100 mg/L, 125 mg/L, 150 mg/L, 175 mg/L, 200 mg/L. The results showed that tryptophan 200 mg/L gave the best concentration in producing the highest alkaloid content of Phaleria macrocarpa callus.
Pertumbuhan dan Uji Kualitatif Kandungan Metabolit Sekunder Kalus Gatang (Spilanthes acmella Murr.) dengan Penambahan PEG untuk Menginduksi Cekaman Kekeringan - Zulhilmi; - Suwirmen; Netty W Surya
Jurnal Biologi Universitas Andalas Vol 1, No 1 (2012)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.1.1.%p.2012

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The study about the growth and qualitative test of secondary metabolite content of the callus culture of Spilanthes acmella Murr. with addition of PEG to induce drought stress had been done used completely randomized design with six treatments and six replications. The treatments were the addition of PEG in various consentration : 1%, 2%, 3%, 4%, 5% and control (without addition of PEG). The result showed that the addition of PEG to medium could decrease fresh weight of callus. The fresh weight of callus was decrease significantly by addition 5% PEG. On the qualitative test of secondary metabolite, alkaloid content was increase by addition of 2% - 5% PEG (++), terpenoid content was increase by addition 3% - 4% PEG (++) and fenolik was found on 4% PEG (+).Keywords: callus culture, secondary metabolite, PEG, drought stress, Spilanthes acmella
Kompatibilitas Spora Glomus Hasil Isolasi dari Rizosfer Macaranga triloba dengan Tiga Jenis Tanaman Inang Gian Wulandari; - Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 3, No 2 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.2.%p.2014

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The study about compatibility of Glomus spores isolated from the rhizosphere Macaranga triloba with three types of host plants have been done from February to November 2013 in greenhouses and Plant Physiology Laboratory, Department of Biology, Andalas University, Padang. The aim of this study was to determine compatibility of corn (Zea mays), jatropha (Jatropha curcas) and scallion (Allium fistulosum) as host plants of Glomus spores isolated from the rhizosphere Macaranga triloba. This experiment used completely randomized design (CRD) with 3 treatments and 9 replications. The treatments were corn, jatropha and scallion. The results showed that spore density of the corn and scallion gave the same effect, whereas jatropha gave different effects on the two plants. The degree of infection of corn roots and scallion showed a high criteria, while the percentage degree of infection on jatropha roots showed a moderate criteria. The corn and scallion were compatible host plants of Glomus spores isolated from the rhizosphere of Macaranga triloba.Key words: compatibility, host plants, Glomus spores
Induksi Embriogenesis Somatik Pada Anggrek Vanda Sumatrana Schltr. dengan Penambahan Beberapa Konsentrasi Asam 2,4-Diklorofenoksiasetat (2,4-D) Anita Tri Astuti; Zozy Aneloi Noli; Suwirmen Suwirmen
Jurnal Biologi Universitas Andalas Vol 7, No 1 (2019)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.7.1.6-13.2019

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The research about induction of somatic embryogenesis of  Orchid Vanda sumatrana Schltr.  by giving 2,4-Diclorophenoxiacetic acid (2,4-D), was conducted from May to July 2016 in plant Physiology and tissue Culture Laboratory, Biology Departement, Matematics and Natural Science Faculty, Andalas University. The aim of this study was found the concentration of 2,4-D to induce somatic embryogenesis of Vanda sumatrana. The research used Completely Randomized Resign (CDR) with 6 treatments and 4 replication. The treatments were : without 2,4-D (control); 1 mg/L; 2 mg/L; 3 mg/L; 4 mg/L; 5 mg/L. The result showed that 2 mg/L 2,4-D and 3 mg/L 2,4-D were concentrations to induct somatic embryogenesis.
Pengurangan Masa Stratifikasi dengan Penambahan Hormon GA3 Pada Perkecambahan Benih Stroberi (Fragaria x annanassa (Weston) Duchesne) Mayola Arda; - Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 3, No 4 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.4.%p.2014

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An experiment on the reduction of stratification period with addition of GA3 hormone to strawberry (Fragaria x annanassa (Weston) Duchesne) seeds germination has been done from December 2013 to February 2014 in the Laboratory of Plant Physiology, Department of Biology, Faculty of Sciences, Andalas University, Padang. The experiment aimed to reduce the stratification period and determine the reliable concentration of GA3 on strawberries seeds germination. The experiment was conducted by using Split Plot Design which consisted of stratification levels (0 week, 1 week and 2 weeks) as main plots, while GA3 consentration (0 ppm, 25 ppm, 50 ppm and 75 ppm) as sub plots. The results from that 25 ppm GA3 combined to 0 week showed the best acceleration to  the emergence of sprouts, increased the number of seed germinations and sprouts length but did not significantly affected to the length of time required for the germination.Key word :GA3, germination, stratification, strawberry
Respon Berbagai Sumber Bahan Stek terhadap Kemampuan Berakar Stek Alstonia scholaris (L) R. Br. sebagai Upaya Penyediaan Bibit untuk Lahan Terdegradasi Kiki Ayunda Putri; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 5, No 1 (2017)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.5.1.1-5.2017

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The research about  the respon  of the material cuttings retrieval on rooting ability of  Alstonia scholaris (L.) R. Br. Cuttings  in an effort provision of seeds for Degraded lands ,  conducted from October until December 2015 at Physiology Plant Laboratorium of Biology Department, Mathematics and Natural Science Faculty of Andalas University, Padang. The aim of this research to found the best  material cuttings on A. scholaris. This research used Completely Randomized Design (CRD) method. The treatments were the basal (A), the middle (B), and the apical (C). The results showed that the apical was the best material cuttings, with a average number of roots (3.967), and the average of root dry weights (0.832)g. The conclusion of this research is the that material cuttings of apical is the bst one for Pulai cutting.
Callus Induction of Aerides odorata Lour. by Adding 2,4 Dichlorophenoxyacetic Acid (2,4-D) Azharia Khalida; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 7, No 2 (2019)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.7.2.109-117.2019

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The research about callus induction of Aerides odorata L. by adding 2,4 Dichlorophenoxyacetic Acid (2,4-D), has been done from August to October 2018 in Plant Physiology and Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was found the effective consentration of 2,4-D to induce somatic embryo of A.odorata. The research used Completely Randomized Design (CRD) with 5 treatments and 6 replications. The treatments were: 0 mg/L 2,4-D; 0,25 mg/L 2,4-D; 0,5 mg/L 2,4-D; 0,75 mg/L 2,4-D; 1 mg/L 2,4-D. The result showed that the treatmeant were able induction callus of A.odorata, with compact until the friable texture, color of the resulting callus is yellowish green and greenish yellow. 2,4-D 1 mg/L was the best concentration to increase fresh weight of callus.