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Peningkatan Kandungan Alkaloid Kalus Mahkota Dewa (Phaleria macrocarpa [Scheff.]Boerl.) Dengan Pemberian Prekursor Triptofan pada Medium Murashige & skoog Wenny Rahma Gusni; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 4, No 1 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.1.%p.2015

Abstract

An experiment aimed to increase alkaloid content of Phaleria macrocarpa callus using tryptophan as a precursor in Murashige & Skoog media was done from September 2013 to January 2014 at the Laboratory of Plant Physiology, Faculty of Sciences, Andalas University. This experiment used Completely Randomized Design (CRD) with 6 treatments and 4 replications. The treatments were 6 consentrations of tryptophan i.e.0 mg/L (control), 100 mg/L, 125 mg/L, 150 mg/L, 175 mg/L, 200 mg/L. The results showed that tryptophan 200 mg/L gave the best concentration in producing the highest alkaloid content of Phaleria macrocarpa callus.
Induksi Perakaran Tunas Tetrastigma rafflesiae Miq. Pada Media Murashige-Skoog Dengan Penambahan Beberapa Konsentrasi Indole-3-Butyric Acid (IBA) secara In Vitro Idris, M.; Suwirmen, -; Febriyanti, Erni
Jurnal Biologi Universitas Andalas Vol 2, No 2 (2013)
Publisher : Universitas Andalas

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Abstract

An experiment on in vitro root induction of Tetrastigma rafflesiae Miq. on Murashige-Skoog (MS) medium suplemented with several concentrations of Indole-3-Butyric Acid (IBA) had been done from February to April 2013. The aims of this experiment was to determine physiological responses of T. rafflesiae’s shoot to the IBA on half strength media of MS for root induction stages. The experiment used a completely randomized design in seven treatments and four replications. The treatment was the addition of IBA in several concentration (i.e. 0.0; 0.5; 1.0; 1.5; 2.0; 2.5 and 3.0 mg/l). The results showed 0.5 mg/l suplemented IBA was more effective and efficient in root induction of shoot then other concentrations. It influenced the average number of roots (12.5 root/plantlet), the average length of root (4.98 cm) and the morphological of plantlets. The result indicated that plantlet of T. rafflesiae generated in in vitro have a higher possibility of artificial culture and give light for future conservation of Rafflesia.Keywords : Tetrastigma rafflesiae Miq., root induction, Indole-3-Butyric-acid.
PENGARUH KONSENTRASI THIDIAZURON (TDZ) DAN ARANG AKTIF PADA SUB KULTUR TUNAS PISANG KEPOK HIJAU (MUSA PARADISIACA L.) Sari, Detty Intan; Suwirmen, Suwirmen; Nasir, Nasril
Natural Science: Journal of Science and Technology Vol 4, No 3 (2015): Volume 4 Number 3 (December 2015)
Publisher : Univ. Tadulako

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Abstract

Penelitian mengenai  ?Pengaruh Beberapa Konsentrasi Thidiazuron (TDZ) dan Arang Aktif pada Sub Kultur Tunas Pisang Kepok Hijau (Musa paradisiaca L.)? telah dilaksanakan pada bulan Agustus sampai November 2014 di Laboratorium Fisiologi Tumbuhan dan Kultur Jaringan, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Padang. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi Thidiazuron, arang aktif dan kombinasi keduanya terhadap pertumbuhan sub kultur tunas pisang kepok secara in vitro. Penelitian ini menggunakan metode eksperimen Rancangan Acak Lengkap (RAL) Faktorial yang terdiri dari dua faktor, faktor pertama adalah pemberian TDZ terdiri dari tiga taraf (0,00; 0,04; 0,09 mg/l) dan faktor kedua adalah pemberian arang aktif yang terdiri dari empat taraf (0,00; 1,00; 2,00; 3,00 g/l) sehingga terdapat 12 kombinasi perlakuan dan tiga kali ulangan. Hasil penelitian menunjukkan bahwa penambahan beberapa konsentrasi TDZ berpengaruh secara signifikan pada jumlah tunas, tidak pada persentase hidup eksplan, tinggi tunas, jumlah akar dan panjang akar. Penambahan beberapa konsentrasi arang aktif memberikan pengaruh yang signifikan pada jumlah dan panjang akar,tidak pada persentase jumlah eksplan, jumlah tunas dan tinggi tunas. Adapun interaksi antara beberapa konsentrasi TDZ dan arang aktif berpengaruh pada parameter panjang akar dengan kombinasi terbaik adalah pada perlakuan 0,04 mg/l TDZ dan 1,00 g/l arang aktif yaitu 6,03 cm
Pertumbuhan dan Uji Kualitatif Kandungan Metabolit Sekunder Kalus Gatang (Spilanthes acmella Murr.) dengan Penambahan PEG untuk Menginduksi Cekaman Kekeringan - Zulhilmi; - Suwirmen; Netty W Surya
Jurnal Biologi Universitas Andalas Vol 1, No 1 (2012)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.1.1.%p.2012

Abstract

The study about the growth and qualitative test of secondary metabolite content of the callus culture of Spilanthes acmella Murr. with addition of PEG to induce drought stress had been done used completely randomized design with six treatments and six replications. The treatments were the addition of PEG in various consentration : 1%, 2%, 3%, 4%, 5% and control (without addition of PEG). The result showed that the addition of PEG to medium could decrease fresh weight of callus. The fresh weight of callus was decrease significantly by addition 5% PEG. On the qualitative test of secondary metabolite, alkaloid content was increase by addition of 2% - 5% PEG (++), terpenoid content was increase by addition 3% - 4% PEG (++) and fenolik was found on 4% PEG (+).Keywords: callus culture, secondary metabolite, PEG, drought stress, Spilanthes acmella
PERTUMBUHAN DAN KADAR ARTEMISININ CINA BARU(Artemisia Vulgaris L). PADA INTENSITAS CAHAYA DAN KOMPOSISI MEDIA TANAM YANG BERBEDA Ivo Octaviani; Zozy Aneloi Noli; Suwirmen Suwirmen
Biocelebes Vol. 10 No. 2 (2016)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The is about the growth and artemisinin content of the Cina Baru (Artemisia vulgaris L.) in different light intensity and growth media composition. It conducted in the Center of Plant Nursery of Andalas University, Padang from March to April 2015. The study used split plot design with 4 treatments and 3 replications, as the main plot was according to the percentage of shading where devided into shading 0% (a0), shading 55% (a1), shading 65% (a2), shading 75% (a3) while sub plot was determined by the  growth media composition: 0 ton/ha (b0), 5 ton/ha (b1), 10 ton/ha (b2), 15 ton/ha (b3). The result showed that light intensity had effect on root growth and number of leaf, while fertilizer significantly influenced the number of leaves, wet weight, net weight. Fertilizer did not significantly have effect on the height of plant and was not able of increase the level of artemisinin, however indicated a strong correlation between net weight and artemisinin level of Artemisia vulgaris L. Keywords: Artemisinin levels, Fertilizer, Light Intensity, Shading
DISEMINASI TEKNOLOGI PAKAN BUATAN ALTERNATIF UNTUK PENGEMBANGAN BUDIDAYA IKAN GURAME INTENSIF DALAM UPAYA MENINGKATKAN PRODUKTIVITAS PERIKANAN DI KELURAHAN LIMAU MANIS KECAMATAN PAUH KOTA PADANG Efrizal Efrizal; Nurmiati Nurmiati; Chairul Chairul; Anthoni Agustien; Suwirmen Suwirmen; Rusnam Rusnam; Deswati Deswati
Jurnal Hilirisasi IPTEKS Vol 3 No 1 (2020)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (739.612 KB) | DOI: 10.25077/jhi.v3i1.347

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Lecturer and community based science and technology activities (IbDM) were carried out with the aim of disseminating technology for Gurame culture intensively in an effort to increase fisheries productivity in Limau Manis, Pauh Subdistrict, Padang City and increase the knowledge and skills of fish farmers (community) in Koto Tangah Village in the field of Gurame fish maintenance business in West Sumatra, so that they are expected to be able to carry out maintenance in a good / environmentally friendly manner. Besides this IbDM activities are also expected to be able to reduce operational costs in maintaining Nirwana Tilapia and in the end can increase the income of the farmers (community) of Limau Manis Village through the development of sustainable fish farming. To achieve optimal technology dissemination results, the main target of IbDM in Pauh Subdistrict is fish farmer groups and community leaders / leaders of Limau Manis Village who are expected to be able to motivate farmers to socialize the method of making alternative formula feeds for intensive development of Nirwana Tilapia aquaculture and environmentally friendly. The methods used in this activity are (1) counseling, with the main material: (a) providing knowledge of various kinds of raw materials that can be used for fish food, (b) providing knowledge about the preparation of artificial food formulas / compositions, ( c) provide knowledge about the process of making artificial food (d) increase public knowledge about how to store pellets that are good to be more durable and (e) provision of material on testing the quality of feed, both physically, chemically and biologically; and (2) Intensive demonstration of making alternative pellet feeds for Nirwana Tilapia aquaculture. From the results of the IbDM activity, it was found that the knowledge and skills of farmer groups in Limau Manis Sub-district, Kec. Pauh, Padang before the IbDM activities were carried out was still very low. However, the motivation or desire of the community of IbDM partner farmer groups to carry out the culture or maintenance of fish is very large. This can be seen from the desire of the IbDM partner farmer group to master the technology of making fish feed from the results of leaf waste and taro stems which are processed into flour relatively high. Furthermore, the results of IbDM activities in the community can increase the knowledge and skills of the community in finding alternative feed for fish feed so that culture activities can run well in IbDM partner areas. In order for aquaculture to run well in partner areas of IbDM, it is necessary to carry out IbDM activities continuously on the techniques of making alternative fish pellets and efficient and effective fish-raising techniques. Besides that, it needs financial support or credit by the authorities to the community or fish farmers for capital to develop their businesses.
Pengaruh Cara Aplikasi dan Konsentrasi Ekstrak Kelor (Moringa oleifera L.) terhadap Pertumbuhan Kubis Singgalang (Brassica oleracea var. capitata L.) Suwirmen Suwirmen; Zozy Aneloi Noli; Fira Julia Putri
Agro Bali : Agricultural Journal Vol 5, No 1 (2022)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.61 KB) | DOI: 10.37637/ab.v5i1.806

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Biostimulan alami adalah salah satu teknologi paling menjanjikan yang baru-baru ini diterapkan dalam sistem pertanian modern yang bertujuan menuju keberlanjutan. Biostimulan didefinisikan sebagai setiap produk yang merangsang efisiensi nutrisi tanaman secara independen dari kandungan nutrisinya dengan tujuan tunggal untuk meningkatkan satu atau lebih karakteristik tanaman atau rizosfer berikut, efisiensi penggunaan nutrisi, toleransi cekaman abiotik, atribut kualitas dan ketersediaan nutrisi. Moringa oleifera mengandung unsur makro dan mikro, zat pengatur tumbuh seperti sitokinin. Penelitian ini bertujuan untuk mengetahui konsentrasi ekstrak kelor dan cara aplikasi ekstrak serta interaksi antara konsentrasi dan cara aplikasi ekstrak yang dapat meningkatkan pertumbuhan dan umur panen kubis Singgalang. Metode yang digunakan adalah Rancangan Acak Lengkap (RAL) Faktorial dengan 2 faktor dan 3 ulangan. Faktor A merupakan taraf konsentrasi (kontrol, 1:64 (v/v), 1:32 (v/v) dan 1:16 (v/v)) dan faktor B merupakan cara aplikasi (daun, tanah dan gabungan daun dan tanah). Hasil penelitian menunjukkan bahwa ekstrak kelor dengan  konsentrasi 1:32 (v/v) mampu meningkatkan jumlah daun dan panjang akar kubis Singgalang. Cara aplikasi ekstrak tidak memberikan pengaruh yang berbeda nyata serta tidak terjadi interaksi antara konsentrasi dan cara aplikasi ekstrak terhadap pertumbuhan dan umur panen kubis Singgalang.   
DISEMINASI TEKNOLOGI BUDIDAYA IKAN NILA NIRWANA INTENSIF DALAM UPAYA MENINGKATKAN PRODUKTIVITAS PERIKANAN DI KELURAHAN LIMAU MANIS KECAMATAN PAUH KOTA PADANG Efrizal Efrizal; Chairul Chairul; Anthoni Agustien; Nurmiati Nurmiati; Zuhri Syam; Suwirmen Suwirmen; Ferry Lismanto Syaiful
Jurnal Hilirisasi IPTEKS Vol 1 No 4.a (2018)
Publisher : LPPM Universitas Andalas

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Kegiatan IPTEKS berbasis dosen & masyarakat (IbDM) ini dilakukan dengan tujuan untuk diseminasi teknologi budidaya ikan Nila Nirwana secara intensif dalam upaya meningkatkan produktivitas perikanan di Kelurahan Limau Manis, Kecamatan Pauh, Kota Padang dan meningkatkan pengetahuan serta keterampilan petani ikan (masyarakat) Kelurahan Koto Tangah dalam bidang usaha pemeliharaan ikan Nila Nirwana di Sumatera Barat, sehingga diharapkan mereka dapat melakukan pemeliharaan dengan cara/teknik yang baik dan ramah lingkungan. Selain itu kegiatan IbDM ini juga diharapakan mampu mengurangi biaya operasional dalam pemeliharaan ikan Nila Nirwana dan pada akhirnya dapat meningkatkan pendapatan para petani (masyarakat) Kelurahan Limau Manis melalui pengembangan usaha budidaya ikan yang berkelanjutan. Untuk mencapai hasil diseminasi teknologi yang optimal maka sasaran utama IbDM di Kecamatan Pauh ini adalah kelompok tani ikan dan para pembina/pemuka masyarakat Kelurahan Limau Manis yang diharapkan mampu memberikan motivasi kepada petani untuk mensosialisasikan metoda pembuatan pakan formula alternatif untuk pengembangan budidaya ikan Nila Nirwana secara intensif dan ramah lingkungan. Metoda yang digunakan dalam kegiatan ini adalah (1) Penyuluhan (ceramah), dengan materi utama : (a) memberikan pengetahuan tentang berbagai macam bahan baku yang dapat dimanfaatkan untuk makanan ikan, (b) memberikan pengetahuan tentang penyusunan formula/komposisi makanan buatan, (c) memberikan pengetahuan tentang proses pembuatan makanan buatan (d) menambah pengetahuan masyarakat tentang cara menyimpan pelet yang baik agar lebih tahan lama dan (e) pemberian materi tentang pengujian mutu pakan, baik secara fisika, kimia dan biologis; dan (2) Peragaan pembuatan pakan pelet alternatif untuk budidaya ikan Nila Nirwana secara intensif. Dari hasil kegiatan IbDM ini didapatkan bahwa pengetahuan dan keterampilan kelompok tani Kelurahan Limau Manis Kec. Pauh, Padang sebelum dilaksanakan kegiatan IbDM ini masih sangat rendah. Namun demikian motivasi atau keinginan masyarakat kelompok tani mitra IbDM untuk melakukan usaha budidaya atau pemeliharaan ikan sangat besar. Hal ini terlihat dari keinginan masyarakat kelompok tani mitra IbDM untuk menguasai teknologi pembuatan pakan ikan dari hasil limbah daun dan batang talas yang diproses menjadi tepung relatif cukup tinggi. Lebih jauh dari hasil kegiatan IbDM pada masyarakat ini dapat menambah pengetahuan dan keterampilan masyarakat dalam mencari pakan alternatif untuk pakan ikan sehingga kegiatan budidaya dapat berjalan dengan baik di daerah mitra IbDM. Agar kegiatan budidaya berjalan dengan baik di daerah mitra IbDM perlu dilakukan kegiatan IbDM secara berkelanjutan tentang teknik pembuatan pelet ikan alternatif dan teknik pemeliharaan ikan yang efisien dan efektif. Disamping itu perlu dukungan dana atau pemberian kredit oleh pihak yang berwenang kepada masyarakat atau petani ikan untuk modal pengembangan usahanya.
Respon Padi Gogo (Oryza sativa L.) terhadap Pemberian Biostimulan dari Ekstrak Rumput Laut Padina minor Zozy Aneloi Noli; Suwirmen Suwirmen; Izmiarti Izmiarti; Reza Oktavia; Putri Aliyyanti
Bioscientist : Jurnal Ilmiah Biologi Vol 9, No 2 (2021): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v9i2.4249

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Currently, seaweed extracts are widely used as plant biostimulants to increase the efficiency of nutrient use, tolerance of abiotic stress, and improve plant quality. In this study, tested the effect of different Padina minor seaweed extract formulas (liquid extract and powder extract) and the frequency of application of Padina minor seaweed extract (b0 = control; b1 = 1 x application; b2 = 2 x application; and b3 = 3 x application) as a biostimulant to the growth of upland rice. From the results of the study, it was found that there was no significant effect of the application of the two extract formulas and the frequency of application, but the administration of Padina minor extract 3 times during vegetative growth increased all growth parameters, such as: plant height, number of tillers, fresh and dry weight of plants, and chlorophyll content. It was concluded that giving Padina minor extract 3 times could increase the vegetative growth of upland rice effectively.
Callus Induction of Aerides odorata Lour. by Adding 2,4 Dichlorophenoxyacetic Acid (2,4-D) Azharia Khalida; Suwirmen Suwirmen; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 7, No 2 (2019)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.7.2.109-117.2019

Abstract

The research about callus induction of Aerides odorata L. by adding 2,4 Dichlorophenoxyacetic Acid (2,4-D), has been done from August to October 2018 in Plant Physiology and Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was found the effective consentration of 2,4-D to induce somatic embryo of A.odorata. The research used Completely Randomized Design (CRD) with 5 treatments and 6 replications. The treatments were: 0 mg/L 2,4-D; 0,25 mg/L 2,4-D; 0,5 mg/L 2,4-D; 0,75 mg/L 2,4-D; 1 mg/L 2,4-D. The result showed that the treatmeant were able induction callus of A.odorata, with compact until the friable texture, color of the resulting callus is yellowish green and greenish yellow. 2,4-D 1 mg/L was the best concentration to increase fresh weight of callus.