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ANTAGONISME FUNGI ENDOFIT DAUN JARAK PAGAR (JATROPHA CURCAS) TERHADAP FUSARIUM OXYSPORUM C2 PENYEBAB BUSUK UMBI PADA BAWANG MERAH IN VITRO Nahdah, Fauziyyah; Sari, Noorkomala; Rizali, Akhmad; Wahdah, Rabiatul
Agrotechnology Research Journal Vol 4, No 1 (2020): Agrotechnology Research Journal
Publisher : Perhimpunan Agroteknologi/Agroekoteknologi Indonesia

Basal plate rot is a major disease on shallot caused by Fusarium oxysporum. Endophytic fungi is promising to use as antagonist agent to the pathogen. Endophyte is microbes that are living in plant cells and have an asymptomatic characteristic. Nowadays, fungal endophyte is believed to produce antimicrobial substances similar with their plant host's natural product. Jatropha curcas is one of the plants containing secondary metabolites that have antifungal activities. The research aimed to study the ability of endophyte from Jatropha curcas to inhibit the growth of Fusarium oxysporum. The dual culture method was used in this research and the data were analyzed by SPSS software. This antagonism test was conducted by 9 isolates endophyte and each plate consisted of 3 replicates. The result revealed endophyte fungal obtaining 9 isolates with the radial growth of 4,5 cm/2 days. Endophytes of Jatropha curcas L. were able to inhibit the growth of Fusarium oxysporum C2. The percentage of inhibition of Fusarium oxysporum causing of root blight diseases was controlled by up 38.27 - 74.48%. The highest percentage of inhibition is gained by B4b and the lowest of it is A2b. Our observations showed that each endophyte has a consistent linear trend. B4b still leaded as the highest strength to inhibit the growth of pathogen on the monitoring of 3, 5, and 7 days. Moreover, the ability of fungi endophyte from Jatropha curcas as antagonist agent to Fusarium oxysporum needs to be further examined by the in vivo method.

Effect of Watering Frequency on The Growth and Yield of Oyster Mushrooms (Pleurotus ostreatus) Saputera, Agus; Sofyan, Antar; Saputra, Riza Adrianoor; Sari, Noorkomala
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 4 No 2 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v4i2.91

Oyster mushrooms have the conditions of growing at low temperatures and high relative humidity, so when you want to cultivate it is needed an appropriate environment for the growth and development of oyster mushroom. Banjarbaru City tends to have high temperatures with a relative humidity that tends to below. This is quite different from the requirements for growing oyster mushrooms which require low temperatures with relatively high humidity. One of the easiest and cheapest methods to maintain temperature and relative humidity to remain ideal and appropriate is to water the surrounding oyster mushrooms growing media, so knowledge of the most appropriate and ideal watering frequency for growth and development of oyster mushrooms is needed. The environmental design used was a Completely Randomized Design (CRD), a single factor with 4 treatments, 5 replications, so that 20 experimental units were obtained. The treatments are p1 = once watering per day, p2 = twice watering per day, p3 = three times watering per day, p4 = four times watering per day. The result showed that the watering frequency showed significant difference to the growth time of oyster mushroom shoots and the wet weight of oyster mushrooms. The best treatment in accelerating the growth time of oyster mushroom shoots and increasing the wet weight of oyster mushrooms is four times watering frequency per day.

Identifikasi dan Uji Patogenisitas Colletotrichum spp. dari Cabai Merah (Capsicum annuum): Kasus di Kricaan, Magelang, Jawa Tengah Noorkomala Sari; Rina Sri Kasiamdari
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 2 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.2.243

The investigation of the agent causing diseases is the first step to determine and control the spread of diseases. Anthracnose causing fruit rot on the red pepper in Indonesia that reduces the yield up to 90%. Colletotrichum is reported as the causative fungal agent of anthracnosis on the red pepper. This study aims to determine the type of Colletotrichum spp. pathogenic fungi on the red pepper collected from Agricultural Field Kricaan, Magelang, Central Java. Methods of identification included macroscopic and microscopic characterization and followed by determining the pathogenicity of the pathogenic agent. In this study, Colletotrichum gloeosporioides and C. acutatum are identified as the causative agents of anthracnose in the red pepper collected from the study location. C. acutatum PC3 strain is identified as the most virulent pathogenic agent. Keywords: anthracnosis, diagnosis, diseases, symptoms, pathogen

Aktivitas Antagonistik Cendawan Endofit Asal Bunga Bawang Dayak (Eleutherine bulbosa (Mill.) Urb.) terhadap Fusarium sp. yang Menginfeksi Tanaman Cabai Noor Laili Aziza; Noorkomala Sari; Sofiya Irsalina
Jurnal Fitopatologi Indonesia Vol 17 No 5 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.5.210-215

Penyakit layu yang disebabkan oleh Fusarium spp. merupakan penyakit penting dalam budi daya cabai. Salah satu cara potensial untuk mengendalikan penyakit tanaman ialah menggunakan agens hayati seperti cendawan endofit dari tanaman obat. Bawang dayak termasuk tanaman obat yang bersifat antibakteri, anticendawan, antiinflamasi, dan antioksidan. Penelitian bertujuan menentukan aktivitas antagonistik cendawan endofit yang berasal dari bunga bawang dayak terhadap cendawan patogen Fusarium spp. Sebanyak tujuh belas isolat cendawan endofit berhasil diperoleh dari bunga bawang dayak, yaitu isolat EnA, EnB, EnC, EnD, EnE, EnF, EnG, EnH, EnI, EnJ, EnK, EnL, EnM, EnN, EnO, EnP, dan EnQ. Lima dari tujuh belas isolat, yaitu EnA, EnF, EnI, EnJ, dan EnK digunakan untuk uji antagonisme terhadap Fusarium spp. dengan metode dual kultur. Penghambatan pertumbuhan koloni Fusarium spp. yang disebabkan oleh isolat EnA, EnF, EnI, EnJ, dan EnK berturut-turut sebesar 67.6%, 53.15%, 77.25%, 70,42% dan 67.1%.

Antibacterial Activity Test of Wet and Dried Extracts of Calabash Tree (Crescentia cujete L.) against Aeromonas hydrophilla Noorkomala Sari; Nengah Dwianita Kuswytasari; Awik Puji Dyah Nurhayati
Biota Vol 6 No 1 (2020): Jurnal Biota 2020
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v6i1.3954

Study to determine the antibacterial activity of wet and dry extract of the leaf, fruit, and bark of Calabash tree (Crescentia cujete L.) against the growth of Aeromonas hydrophila. The solvent extraction process was done by using 96% ethanol in the maceration method. Antibacterial test results using diffusion agar to decide clear zone and tube series of dilution test to provide MIC and MBC. Fresh leaf extract produces the highest clear zone diameter (20.06 mm), after which fresh bark extract (12.81 mm), and the last is fresh fruit extract (3.22 mm). In contrast to fresh extracts, the dried extracts are have not clear zone. MIC (Minimum Inhibitory Concentration) of Calabash Tree fresh leaf extract against Aeromonas hydrophila is 80%, and MBC (Minimum Bactericidal Concentration) is 100%.

Review of Endophytic Fungi as Biocontrol Agents Against Plant Pathogen Noorkomala Sari
Gontor AGROTECH Science Journal Vol 6, No 1 (2020): June 2020
Publisher : University of Darussalam Gontor, Ponorogo, East Java Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/agrotech.v6i1.3734

Fungi endofit adalah fungi yang hidup pada inter dan intra sel tumbuhan sehat dan tanpa menunjukkan gejala penyakit pada tumbuhan tersebut. Hubungan endofit dengan tumbuhan inangnya direferensikan sebagai hubungan mutualisme dimana fungi endofit menghasilkan mikotoksin dan metabolit sekunder lainnya yang mengakibatkan perubahan fisiologi dan biokimia sel inang sehingga menghambat perkembangan patogen tumbuhan dan fungi endofit mendapatkan nutrisi dari tumbuhan inangnya. Karena kemampuan fungi endofit yang menginduksi respon metabolisme tumbuhan inang sehingga tumbuhan menjadi resisten terhadap patogen tanaman, telah banyak dilakukan penelitian menggunakan fungi endofit dengan cara diinokulasikan pada tanaman komoditas untuk pengendalian persebaran penyakit pada lahan pertanian. Endofit sebagai agen biokontrol, memiliki pengaruh yang besar dalam meningkatkan produktivitas tanaman dan disarankan oleh beberapa ahli ekologi tanaman sebagai biopestisida dalam mewujudkan pertanian berkelanjutan yang ramah lingkungan.

Effect of Watering Frequency on The Growth and Yield of Oyster Mushrooms (Pleurotus ostreatus) Agus Saputera; Antar Sofyan; Riza Adrianoor Saputra; Noorkomala Sari
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 4 No 2 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v4i2.91

Oyster mushrooms have the conditions of growing at low temperatures and high relative humidity, so when you want to cultivate it is needed an appropriate environment for the growth and development of oyster mushroom. Banjarbaru City tends to have high temperatures with a relative humidity that tends to below. This is quite different from the requirements for growing oyster mushrooms which require low temperatures with relatively high humidity. One of the easiest and cheapest methods to maintain temperature and relative humidity to remain ideal and appropriate is to water the surrounding oyster mushrooms growing media, so knowledge of the most appropriate and ideal watering frequency for growth and development of oyster mushrooms is needed. The environmental design used was a Completely Randomized Design (CRD), a single factor with 4 treatments, 5 replications, so that 20 experimental units were obtained. The treatments are p1 = once watering per day, p2 = twice watering per day, p3 = three times watering per day, p4 = four times watering per day. The result showed that the watering frequency showed significant difference to the growth time of oyster mushroom shoots and the wet weight of oyster mushrooms. The best treatment in accelerating the growth time of oyster mushroom shoots and increasing the wet weight of oyster mushrooms is four times watering frequency per day.

Limbah Baglog Jamur Tiram Putih sebagai Kompos pada Cabai Rawit (Capsicum frutescens L.) Var. Hiyung Nur H D Ayu; Jumar Jumar; Noorkomala Sari
JURNAL BUDIDAYA PERTANIAN Vol 17 No 1 (2021): Jurnal Budidaya Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jbdp.2021.17.1.83

Chili pepper is a plant that is favored by the community because its fruit has a spicy taste, so it is widely cultivated by farmers. One of the chili pepper varieties in Indonesia is Hiyung cayenne pepper which originally comes from Hiyung Village of Tapin Tengah District, Tapin Regency, South Kalimantan. In plant-cultivation, fertilization has an important role to meet the nutrient needs of plants. Fertilization to reduce environmental pollution can be done by using organic fertilizers, for example by giving compost of white oyster mushroom planting media waste (baglog). The purpose of this study was to know the effect and the best dose of application of white oyster mushroom baglog waste compost on the growth of cayenne pepper (Capsicum frutescens L.) variety Hiyung. The research was carried out in February-May 2020, at the Hortibun Agroecotechnology Experimental Field, Faculty of Agriculture, Lambung Mangkurat University. The method used in this study was a Completely Randomized Design (CRD) with one factor. The treatments tested consisted of: k0 = control (without baglog waste compost) or 0 tons/ha; k1 = 5 tons/ha of baglog waste compost or 125 g/planting hole; k2 = 10 tons/ha of mushroom baglog waste compost or 250 g/planting hole, k3 = 15 tons/ha of mushroom baglog waste compost or 375 g/planting hole; and k4 = 20 tons/ha of baglog compost waste or 500 g/ planting hole. The treatment was repeated 4 times so that there were 20 experimental units. The results on the parameters of plant height, number of leaves, and productive branches showed that the application of white oyster mushroom baglog waste compost had an effect on the growth of cayenne pepper variety Hiyung. The best treatment recommendation was the K3 treatment (15 tons/ha or 375 g/planting hole) which was able to increase plant height by 55.9%; number of leaves 88.2%; and 160% productive branch compared to control at 42 days after planting. Keywords: Hiyung cayenne chilli, baglog waste compost, organic fertilizer, vegetative stage ABSTRAK Cabai merupakan tanaman yang disukai masyarakat karena buahnya memiliki cita rasa pedas, sehingga banyak dibudidayakan oleh pada petani. Salah satu varietas cabai yang ada di Indonesia, yaitu cabai rawit Hiyung, berasal dari Desa Kecamatan Tapin Tengah, Kabupaten Tapin, Kalimantan Selatan. Dalam kegiatan budidaya tanaman, pemupukan memiliki peran yang penting untuk memenuhi kebutuhan unsur hara bagi tanaman. Pemupukan dengan tujuan mengurangi pencemaran lingkungan dapat dilakukan dengan pengunaan pupuk organik, misalnya pemberian kompos limbah media tanam jamur tiram putih (baglog). Tujuan penelitian adalah mengetahui pengaruh dan dosis terbaik aplikasi kompos limbah baglog jamur tiram putih pada pertumbuhan cabai rawit (Capsicum frutescens L) varietas Hiyung. Pelaksanaan penelitian pada Februari 2020-Mei 2020, di Kebun Percobaan Hortibun Agroekoteknologi Fakultas Pertanian Universitas Lambung Mangkurat. Metode yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) satu faktor. Perlakuan yang diuji terdiri dari: K0 = kontrol (tanpa kompos limbah baglog ) atau 0 ton/ha; K1 = 5 ton/ha kompos limbah baglog atau 125 g/lubang tanam; K2 = 10 ton/ha kompos limbah baglog atau 250 g/lubang, K3 = 15 ton/ha kompos limbah baglog atau 375 g/lubang tanam dan K4 = 20 ton/ha kompos limbah baglog atau 500 g/lubang tanam. Perlakuan diulang 4 kali sehingga terdapat 20 satuan percobaan. Hasil pada parameter tinggi tanaman, jumlah daun, dan cabang produktif menunjukan bahwa aplikasi kompos limbah baglog jamur tiram putih berpengaruh pada pertumbuhan cabai rawit varietas Hiyung. Rekomendasi perlakuan terbaik terdapat pada perlakuan K3 (15 ton/ha atau 375 g/lubang tanam) yang mampu meningkatkan tinggi tanaman 55,9%; jumlah daun 88,2%; dan cabang produktif 160% dibandingkan dengan kontrol pada 42 Hari Setelah Tanam (HST). Kata kunci: Cabai rawit Hiyung, kompos limbah baglog, pupuk organik

PERTUMBUHAN DAN PRODUKSI BAWANG MERAH (Allium ascalonicum L.) YANG DIAPLIKASIKAN PUPUK KANDANG DAN BOKASHI KIAMBANG Khadijah Khadijah; Akhmad Rizali; Noorkomala Sari
Jurnal Pertanian Vol. 12 No. 2 (2021): OKTOBER
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jp.v12i2.4264

Produktivitas tanaman bawang merah di Indonesia dari tahun 2013 hingga 2017 berfluktuasi setiap tahunya, hal ini disebabkan oleh faktor sarana produksi dalam budidaya yaitu kualitas lahan. Kesuburan lahan di Kalimantan Selatan mengalami penurunan akibat degradasi lahan akibat input bahan kimia pada kegiatan pertanian secara berlebihan, diperlukan input bahan organik. Tujuan dari penelitian ini adalah untuk mengetahui perbedaan respon pertumbuhan dan hasil tanaman bawang merah dari kombinasi beberapa jenis pupuk kandang dan dosis bokashi kiambang, untuk mengetahui apakah ada interaksi antara pupuk kandang dan bokashi kiambang terhadap pertumbuhan dan hasil tanaman bawang merah (Allium ascalonicum L.). Hasil penelitian parameter tinggi tanaman, jumlah daun, berat basah dan berat kering menunjukkan perbedaan yang nyata antara perlakuan terhadap pertumbuhan dan produksi bawang merah (Allium ascalonicum L.) dari perlakuan kombinasi beberapa jenis pupuk kandang dan dosis bokashi kiambang. Sedangkan parameter jumlah umbi tidak signifikan. Rekomendasi perlakuan terbaik adalah K1B1a, baik dalam meningkatkan tinggi tanaman, bobot basah dan bobot kering dan K1B2a baik untuk menambah jumlah daun dan jumlah umbi.

Pengenalan Budidaya Padi System of Rice Intensification (SRI) di Desa Sungai Rangas Kabupaten Banjar Untung Santoso; Riza Adrianoor Saputra; Antar Sofyan; Rabiatul Wahdah; Noor Khamidah; Noorkomala Sari; Muhammad Imam Nugraha; Muhammad Aulia Mahendra; Juanda Saputra
Jurnal Pengabdian ILUNG (Inovasi Lahan Basah Unggul) Vol 1, No 3 (2022)
Publisher : Universitas Lambung Mangkurat

Desa Sungai Rangas Kabupaten Banjar merupakan salah satu daerah lumbung pangan di Kalimantan Selatan dengan hasil panen padi yang belum optimal karena masih menggunakan metode pertanian konvensional. Hal ini mendasari perlunya pengenalan Sistem pertanian organik dengan metode System of Rice Intensification (SRI) sebagai alternatif dalam pengembangan budidaya padi di Desa Sungai Rangas, tujuannya selain pertanian berbasis ramah lingkungan juga dapat mendukung pertanian berkelanjutan. Kegiatan pengabdian kepada masyarakat dalam rangka mengenalkan SRI kepada petani sasaran Desa Sungai Rangas dilaksanakan pada Rabu, 22 September 2021. Metode kegiatan pengabdian kepada masyarakat yaitu metode ceramah dan diskusi yang didasari oleh hasil observasi permulaan sebagai landasan penentuan tingkat pemahaman dan wawasan petani mengenai budidaya padi metode SRI. Alat pengukuran berupa kuisioner berisi daftar pertanyaan akan manfaat dan pengetahuan materi yang diberikan hal ini bertujuan untuk mengetahui peningkatan pengetahuan peserta kegiatan terhadap materi penyuluhan yang telah diinformasikan. Hasil dari kegitan ini yaitu seluruh peserta penyuluhan di Desa Sungai Rangas mengetahui tentang budidaya padi metode SRI (System of Rice Intensification) sedangkan evaluasi dari kegiatan ini yaitu akomodasi waktu yang lebih banyak dan penambahan acara praktek di persawahan dalam hal mempraktekan cara menaman dengan metode SRI di lahan persawahan Sungai Rangas agar peserta dapat langsung mempraktekannya sehingga dapat mengenali kendala dan tantangan secara langsung dalam menerapkan metode ini. Kedepannya peserta penyuluhan ingin mencoba menerapkan budidaya padi metode SRI di lahan sawah mereka. Kata kunci: sistem tanam, padi, peningkatan hasil, penyuluhan, pertanian berkelanjutan

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