Claim Missing Document
Check
Articles

Found 15 Documents
Search

PERBANDINGAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN SIRSAK (Annona muricata L.) BERDASARKAN TEMPAT TUMBUH DENGAN METODE PEREDAMAN DPPH Aminah Aminah; St Maryam; Muzakkir Baits; Ummi Kalsum
Jurnal Fitofarmaka Indonesia Vol 3, No 1 (2016): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1248.635 KB) | DOI: 10.33096/jffi.v3i1.175

Abstract

Antioxidant activity assay has been conducted in order to determine the comparison of the antioxidant activity contained in the ethanol extract of the soursop leaves (Annona muricata L.) from Makassar, north Mamuju and Jeneponto. The amount of antioxidant activity obtained by testing against free radical DPPH (1,1-Diphenyl-2-picryl Hydrazyl) at concentrations of 10, 20, 30, 40 dan 50 ppm with 96% ethanol. The testing was done with the addition of 1 mL of the test solution with 4 mL DPPH 35 ppm. The absorbance of free radical activity was measured by UV-Vis spectrophotometer at the wavelength 515 nm and the IC50 value was calculated based on the absorbance data. The calculations showed that the soursop leaves from Makassar, North Mamuju, and Jeneponto had the IC50 value of 1.380 µg/mL, 1.512, and 1.420 respectively. The results showed that the samples came from Makassar had a higher antioxidant activity.
PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK ETANOL KULIT BUAH ALPUKAT (Persea americana Mill.) DENGAN METODE SPEKTROFOTOMETRI UV-VIS Aminah Aminah; Nurhayati Tomayahu; Zainal Abidin
Jurnal Fitofarmaka Indonesia Vol 4, No 2 (2017): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (890.82 KB) | DOI: 10.33096/jffi.v4i2.265

Abstract

Avocado plant (Persea americana Mill.), belongs to Lauraceae family which have medicinal properties. Avocado peel contains flavonoid compounds that can be used to protect and reduce the skin damage against UV rays. This study aimed to determine the total flavonoids content of the ethanol extract of the avocado peel using UV-Vis spectrophotometry. The ethanol extract of avocado peel was obtained by maceration with ethanol. The qualitative analysis used FeCl3 by forming green complex. The determination of total flavonoids content is conducted based on AlCl3 method with total flavonoids expressed in QE (Quercetin equivalent) at the maximum wavelength of 435 nm. The result showed that the average content of flavonoid total is 4.0122 mgQE/g extract.
POTENSI SENYAWA FLAVONOID DAUN AFRIKA (Vernonia amygdalina Del.) ASAL TERNATE SEBAGAI ANTIOKSIDAN Sukmawati Sukmawati; Harira Hadi; Aminah Aminah
As-Syifaa Jurnal Farmasi Vol 9, No 2 (2017): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.555 KB) | DOI: 10.33096/jifa.v9i2.278

Abstract

African Leaf (Vernonia amygdalina Del.) belonging Asteraceae family. The African leaf contain flavonoids, alkaloids, saponins, terpenoids, tannins, glycosides, indole alkaloids, anthraquinones and luteolin. This study aimed to measure the potencyl of flavonoid compounds of African leaf (Vernonia amygdalina Del.) origin Ternate using DPPH and FRAP. Extraction by maceration method using ethanol 96%. The compounds identification in African leaf extract contains flavonoids. The absorbance was measured at the maximum wavelength of 515 nm. DPPH scavenging showed that ethanol extract has weak antioxidant activity based on AAI 0.454 value. This potency is lower in comparasion with quersetin which has a value of AAI 6,315 and for FRAP methods, the absorbance was measured at the wavelength of 720 nm with the antioxidant capacity is 14,846 mgQE / gram.
POTENSI EKSTRAK RUMPUT LAUT (Eucheuma cottoni) SEBAGAI ANTIOKSIDAN Aminah Aminah; Hamsinah Hamsinah; Nurmila A. Abiwa; Sulasmi Anggo
As-Syifaa Jurnal Farmasi Vol 12, No 1 (2020): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.849 KB) | DOI: 10.33096/jifa.v12i1.600

Abstract

Seaweed (Eucheuma cottoni) is a marine biota that is often used as an additive to pharmaceutical products that function as thickener. But seaweed, which is rich in carrageenan, also contains vitamin C which has the potential as an antioxidant. The method used was to test the antioxidant activity of seaweed extract (Eucheuma cottoni) with the DPPH (1,1-Diphenyl-2-Picrylhdrazil) damping method. The test sample was in the form of seaweed ethyl acetate extract, where the standard standard of comparison of quarsetin and seaweed extract samples was made in 5 variations of concentration. The standard curve produced by quarsetin with concentrations of 2, 4, 6, 8, 10 ppm at the maximum wavelength DPPH 514,942 nm. The results obtained by the Kuarsetin standard with regression values y = 6.4765x + 7.567, the correlation value r = 0.99959 and IC50 = 6.48 (very strong antioxidant activity) while the ethyl acetate extract of seaweed with concentrations of 600, 800, 1000, 1200, 1400 ppm % inhibition was obtained with regression values y = 0.0104x + 16.708, the correlation value r = 0.9972 and IC50 = 3201.15 (very weak antioxidants). So it can be concluded that the seaweed ethyl acetate extract has the potential for antioxidant activity.
STANDARISASI EKSTRAK DIETIL ETER HERBA Oxalis corniculata L. Herwin Herwin; Aminah Hamzah
As-Syifaa Jurnal Farmasi Vol 4, No 2 (2012): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.687 KB) | DOI: 10.33096/jifa.v4i2.78

Abstract

A research have been conducted about standarisation of  diethyl ether ekstrack Oxalis corniculata L. herb from West Sulawesi of Mamuju with a purpose standarisation of  diethyl ether ekstrack Oxalis corniculata L. based on specific and non specific parameter. On the way KLT-Bioautography diethyl ether ekstrack with Rf 0.47 value to give activity to Staphylococcus aureus, Streptococcus mutans, Staphylococcus epidermidis, Salmonella thypi, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Vibrio sp, and Candida albicans. Based on standarisation to result diethyl ether ekstrack obtained that specific parameter to organoleptic fixing ekstrac is green chocolate, typically smelling, bitter  feel, jell konsistance, dissolve compound rate of ethanol 95% counted 31.37%, dissolve compound rate of water counted 24.00% and non specific parameter to stipulating on water rate counted 16.66%, dusty rate counted 4.16%, sour insoluble dusty rate counted 0.41%, total determination of bacterium 10x102 koloni/g and total kapang 37x101koloni/g. Key word : Oxalis corniculata L. Herb, Standarisation of the Diethyl Ether Ekstrack, Antimicrobial 
ANALISIS KADAR ARSEN (As) DAN TIMBAL (Pb) PADA MINYAK GORENG PEMAKAIAN BERULANG DENGAN METODE SPEKTROFOTOMETRI SERAPAN ATOM Aminah Aminah; Rahmawati Rahmawati; Tadjuddin Naid; Salma Salma
As-Syifaa Jurnal Farmasi Vol 9, No 1 (2017): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.96 KB) | DOI: 10.33096/jifa.v9i1.235

Abstract

This study aims to analyze the levels of arsenic (As) and lead (Pb) in frying oil usage over and over. This research was conducted at the Center for Health Laboratory Makassar. Samples obtained from merchants fried foods. To ensure the metal content of the samples prior didestruksi with HNO3P and heated for 16 hours to liberate arsenic and lead from the oil matrix, then measured using atomic absorption spectrophotometer (AAS) at a wavelength of 193.7 nm (Arsenic) and 283 nm (lead ). From the results obtained penilitian arsenic (As) in the three vendors that is <0.1 ppm (max 0.1 ISO ppm) and lead levels that merchants 1 (0.1503 mg / kg and 0.1940 mg / kg), traders 2 (0.3452 mg / kg and 0.4005 mg / kg), the merchant 3 (0.1111 mg / kg and 0.1788 mg / kg) respectively for the samples before and after frying (SNI max 0.1 mg / kg).
Validasi Metode Analisis Cemaran DNA Babi pada Bakso Sapi Menggunakan Primer Mitokondria D-Loop22 dengan Metode Polymerase Chain Reaction (PCR): Analysis Method Validation of Pig DNA Contamination in Cow Meatballs Using Mitochondrial Primer D-Loop22 by Polymerase Chain Reaction (PCR) Method Sri Wahyuni; Siti Maryam; Aminah Aminah
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 5 No. 1 (2019): (March 2019)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.799 KB) | DOI: 10.22487/j24428744.2019.v5.i1.12035

Abstract

The frequency of non-halal ingredient mixing, such as porkin on the food processed products of meatballs, has become an issue to the public, especially for moslems. Therefore, a reliable and valid method with high sensitivity is needed to specifically detect the pig contamination. This research aims to obtain a valid and reliable method by proposing polymerase chain reaction (PCR) using mitochondrial D-Loop22 primer as a method in handing halal food authentication. The sample consisted of beef as a negative control, pork and pork meatballs as a positivecontrol, and five samples of meat balls found in Makassar for halal inspection.The method validation assay was conducted by testing the primary specificity on the fresh tissue (beef and pork) and testing the sensitivity by making a series of pig DNA dilution (1:10; 1:102; 1:103; 1:104) and the variations of contaminated pig:cow (%b/b) : 0.05% , 0.1%, 1%, and 5%. The result of PCR amplification on agarose gel electrophoresis of 0.8% showed that method was able to detect the pig DNA contamination specifically in pigs and not amplify other DNA, and could still be detected up to pig contamination specifically in pigs and not amplify the other DNAs and could still be detected up to pig contamination of 0.05% and on DNA dilution of 1:103. Meanwhile, on the five samples analyzed, there were not found pig DNA contamination characterized by no formed amplification bands.
Analisis Cemaran DNA Tikus pada Bakso Daging Sapi yang Beredar di Makassar dengan Metode Polymerase Chain Reaction (PCR): Analysis of Rat DNA Contamination on Beef Meatballs Circulating in Makassar by Polymerase Chain Reaction (PCR) Method Aminah Aminah; Ristieyen Ramadini; Tadjuddin Naid
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 5 No. 1 (2019): (March 2019)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.012 KB) | DOI: 10.22487/j24428744.2019.v5.i1.12036

Abstract

Analysis of rat DNA contamination in meatball meat circulating in Makassar by PCR (Polymerase Chain Reaction) method has been carried out. In this study, a polymerase chain reaction method will be developed to analyze the presence of rat meat contamination in beef meatballs. There are three stages in the PCR amplification process carried out with 30 cycles, which are 95oC temperature denaturation, 51oC attachment, and 72oC extension. DNA analysis included agarose gel electrophoresis, measurement of concentration and purification, and analysis of rat DNA using PCR. The results of PCR amplification using mouse-specific primers namely primary ND1 (NADH dehydrogenase 1) showed no bands seen in UV light. So that it can be proven that beef meatball samples in the Makassar region did not contain rat DNA. t.
Uji Aktivitas Antioksidan dan Profil Fitokimia Ekstrak Kayu Beta-beta (Lunasia amara Blanco.): Antioxidant Activity and Phytochemical Profile of Beta-beta (Lunasia amara Blanco) Wood Extract Hasnaeni Hasnaeni; Aminah Aminah
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 5 No. 1 (2019): (March 2019)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.43 KB) | DOI: 10.22487/j24428744.2019.v5.i1.12404

Abstract

Beta-beta wood (Lunasia amara Blanco) is one of the Rutaceae plants. It has been used traditionally in Indonesia, both in the form of a single extract or in a mixture of several herbs, for the treatment of swollen feet, skin diseases and inflammation or irritation of the eyes. This study aims to examine the antioxidant activity and the phytochemical profile of beta-beta wood extract. The research methods include plant extraction by maceration, testing of antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and phytochemical profiling with specific chemical reagents. The results showed that beta-beta wood extract (Lunasia amara Blanco) has antioxidant activity with IC50 was 69.46 μg/ml (0.069 mg/ml) and it contained steroid, phenolic, saponin, alkaloid and coumarin compounds.
Pembuatan Teh Seduh Herbal Dari Daun Kelor (Moringa oleifera) Sebagai Peningkat Imunitas Tubuh Di SMAN 13 Maros Kecamatan Tompobulu Kabupaten Maros Hamsinah Hamsinah; Asriani Suhaenah; Nurmaya Effendy; Aminah Aminah; Inayanti Fatwa
Dikmas: Jurnal Pendidikan Masyarakat dan Pengabdian Vol 2, No 1 (2022): March
Publisher : Magister Pendidikan Nonformal Pascasarjana Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/dikmas.2.1.103-110.2022

Abstract

Sekolah merupakan salah satu tempat yang beresiko terhadap penularan kasus Covid-19. Salah satu upaya yang dapat dilakukan untuk pencegahan penyebaran virus Covid-19 adalah peningkatan nutrisi dalam tubuh untuk meningkatkan imunitas tubuh dengan mengkonsumsi daun kelor. Daun kelor merupakan salah satu tanaman yang dapat dikembangkan secara produktif khususnya di bidang pangan. Tujuan kegiatan ini adalah meningkatkan pengetahuan dan wawasan siswa dan guru terkait manfaat daun kelor, serta peningkatan pengetahuan pembuatan daun kelor menjadi produk teh seduh. Metode pelaksanana kegiatan pengabdian dilaksanakan secara yang dihadiri oleh pengurus OSIS sebanyak 35 siswa di sekolah SMAN 13 Maros Kecamatan Puca Kabupaten Maros.