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Journal : Jurnal Fitofarmaka Indonesia

PERBANDINGAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN SIRSAK (Annona muricata L.) BERDASARKAN TEMPAT TUMBUH DENGAN METODE PEREDAMAN DPPH Aminah Aminah; St Maryam; Muzakkir Baits; Ummi Kalsum
Jurnal Fitofarmaka Indonesia Vol 3, No 1 (2016): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1248.635 KB) | DOI: 10.33096/jffi.v3i1.175

Abstract

Antioxidant activity assay has been conducted in order to determine the comparison of the antioxidant activity contained in the ethanol extract of the soursop leaves (Annona muricata L.) from Makassar, north Mamuju and Jeneponto. The amount of antioxidant activity obtained by testing against free radical DPPH (1,1-Diphenyl-2-picryl Hydrazyl) at concentrations of 10, 20, 30, 40 dan 50 ppm with 96% ethanol. The testing was done with the addition of 1 mL of the test solution with 4 mL DPPH 35 ppm. The absorbance of free radical activity was measured by UV-Vis spectrophotometer at the wavelength 515 nm and the IC50 value was calculated based on the absorbance data. The calculations showed that the soursop leaves from Makassar, North Mamuju, and Jeneponto had the IC50 value of 1.380 µg/mL, 1.512, and 1.420 respectively. The results showed that the samples came from Makassar had a higher antioxidant activity.
PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK ETANOL KULIT BUAH ALPUKAT (Persea americana Mill.) DENGAN METODE SPEKTROFOTOMETRI UV-VIS Aminah Aminah; Nurhayati Tomayahu; Zainal Abidin
Jurnal Fitofarmaka Indonesia Vol 4, No 2 (2017): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (890.82 KB) | DOI: 10.33096/jffi.v4i2.265

Abstract

Avocado plant (Persea americana Mill.), belongs to Lauraceae family which have medicinal properties. Avocado peel contains flavonoid compounds that can be used to protect and reduce the skin damage against UV rays. This study aimed to determine the total flavonoids content of the ethanol extract of the avocado peel using UV-Vis spectrophotometry. The ethanol extract of avocado peel was obtained by maceration with ethanol. The qualitative analysis used FeCl3 by forming green complex. The determination of total flavonoids content is conducted based on AlCl3 method with total flavonoids expressed in QE (Quercetin equivalent) at the maximum wavelength of 435 nm. The result showed that the average content of flavonoid total is 4.0122 mgQE/g extract.