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All Journal Katalogis J@TI (TEKNIK INDUSTRI) ELKHA : Jurnal Teknik Elektro AKTUALITA GARIS Jurnal Mahasiswa Jurusan Arsitektur BERITA BIOLOGI Rang Teknik Journal Preventif Journal Penelitian Pendidikan Geografi Biosaintifika: Journal of Biology & Biology Education Jurnal Kolaboratif Sains JTT (Jurnal Teknologi Terapan) Jurnal Sains dan Teknologi Reaksi Stabilita : Jurnal Ilmiah Teknik Sipil Fakultas Teknik Universitas Halu Oleo Kendari Journal of Robotics and Control (JRC)
Ishak Ishak
Centre for Isotopes and Radiation Application, BATAN, Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Physics Transportation Other

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Journal : Biosaintifika: Journal of Biology

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Identification of Heading Date Six (Hd6) Gene Derived from Rice Mutant Varieties Aryanti, Aryanti; Dwimahyani, Ita; Ishak, Ishak
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.9210

Abstract

Genes which were associated with flowering time to indicate the early maturity is known as heading date (Hd). Heading date six (Hd6) gene was identified from rice mutant varieties were Atomita 2, Atomita 3, Atomita 4, Bestari, Cilosari, Diah Suci, Sidenuk, Kahayan, Mayang, Meraoke, Mira-1, Pandan Putri, Superwin, Suluttan Unsrat 1, Suluttan Unsrat 2, Winongo, Woyla, Yuwono, while the rice var. Nipponbare was used as a positive control. All of rice mutant varieties derived from mutation induction by the dose of 0.2 kGy. The aim of this experiment was to find out the data base of mutant varieties which could be used as parent material with earlier maturity trait genetically. To obtain the DNA of plants, young leaves of each variety were extracted by liquid nitrogen, and then lysis and extracted by Kit Plant Genomic DNA. The amplification of DNA with 7 primers of Hd6 conducted of 40 cycles by PCR and were continues to separated by 1 % agarose. The results were shown that the rice Mira-1 and Bestari varieties obtained from mutation of Cisantana highly different from one to another on 7 primers of Hd6 used. Mayang variety from mutation of cross breeding between Cilosari and IR64, Pandan putri from Pandan wangi and Woyla from mutation of cross breeding from Atomita 2 and IR64 were highly different with those of their parents. Identification of Hd6 gene on Sidenuk variety was shown the same bands pattern with Nipponbare as control positive toward all primers used, this variety would be better for earlier maturity parent material compared to others. The information could be useful for breeding programs aiming to develop early maturing widely adaptive and high yielding rice cultivars.
Co-Authors A.A. Ketut Agung Cahyawan W Adris Ade Putra Aryanti Aryanti Budiman Budiman Cosmas Andi Wiraatmaja, Cosmas Andi Dedi Setiawan Dzar Faraby, Muhira Fachrul, Muhammad Gandanegara, Soertini Helena J Kristina Ita Dwimahyani Jamaluddin Sakung Magribi, La Ode Muh. Muhammad Ardiansyah Sembiring Muhammad Iswar Radinal, Radinal Radinal, Radinal ramadan, sachrul Rukiah Rukiah Setiawan Setiawan SITTI ROSYIDAH, SITTI Sobrizal Sobrizal Suwandi N