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Bambang Sutrisno
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Journal : Jurnal Veteriner

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Deteksi Bovine Herpesvirus-1 Secara Immunohistokimia pada Membran Korioallantois Telur Ayam Berembrio (IMMUNOHISTOCHEMISTRY DETECTION OF BOVINE HERPESVIRUS-1 IN CORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYONATED EGG) Yuli Purwandari Kristianingrum; Charles Rangga Tabbu; Bambang Sutrisno; Sitarina Widyarini; Kurniasih .; Tri Untari; Asmarani Kusumawati
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Infectious Bovine Rhinotracheitis (IBR) is caused by Bovine Herpes virus-1 in the cattle. The clinicalsigns demonstrate depression, anorexia, swelling of the vulva, redness of the vestibule, pustule and ulceron the vaginal mucosal. Based on previous research, IBR virus from the nasal swab could be grown inchorio-allantoic membrane of embryonated chicken eggs. This study aim was to confirm whether IBR virusin cattle could be grown in embryonated chicken eggs as a substitute for cell culture. A total of five nasalswab samples from the cows that were positive for IBR infection (diagnosed by Polymerase Chain Reactionand cell culture) were inoculated on the chorio-allantois membrane of embryonated chicken eggs.Observation of lesions performed at 3-5 days after inoculation. Re-inoculation (passage) was done threetimes. Pock characteristic lesions were observed on the corioallantoic membrane with the size of 5-7 mm,rounded shape, opaque edge, with necrosis in the central area. Furthermore, pock lesions were processedfor hematoxylin and eosin staining and immuno-histochemistry. The result of hematoxylin and eosinstaining showed that the formation of intranuclear inclusion bodies and vacuolization of the epithelial cellof membrane was observed. Immuno-histochemistry staining showed positive reaction for antibodiesagainst BHV-1 in the epithelial cells membrane. In conclusion, embryonated chicken eggs could be usedas a medium for detection of IBR.
Identifikasi Leptin pada Kesembuhan Luka Tikus yang Diberi Pakan Lemak Tinggi dan Aplikasi Zinc Topikal (LEPTIN IDENTIFICATION ON WOUND HEALING OF RAT GIVEN HIGH FAT DIET AND TOPICAL ZINC APPLICATION) Devita Anggraeni; Dhirgo Adji; Bambang Sutrisno
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Leptin is a hormone produced by adipocytes and play an important role in wound healing. The objectiveof this research was to study the role of leptin in wound healing in mice experimentally given high fat dietsand its correlation to zinc. Thirty two male Sprague Dawley rats at three months of age were used in thisstudy. Rats were randomly allotted into four groups (A,B,C and D) of 8. Rats in group A and B were fednormal diet, while rats in group C and D were fed high fat diet. After two months of treatment, skin incisionsurgery was performed at the back side of the rat. Incision wound was closed with single interruptedsuture. The wound of rats in group A and C were treated with vaseline, while in the group B and D weretreated with zinc 10%. One day after surgery, blood sample were collected frpm four rats from each groupand analysed for leptin (Rat leptin ELISA Int, Genway Biotech Inc). Wounded skin from all animals werealso taken for histopathological examination (Haematoxylin and Eosin). Three days after the surgery, thesame analysis were done for the remaining rats. Leptin level was analyzed statistically using ANOVA forfactorial experiment, while histopathologic analysis was done descriptively. The results showed that theleptin level was significantly affected by time (P<0.05), as leptin level in blood at three days after surgerywas significantly lower than the level at one day after surgery. Meanwhile, histopathological examinationshowed that the percentage of epidermal closure in animals in group A,B,C,and D were 75%, 100%, 25%and 75%, respectivelly. Therefore, it was concluded that topical application of zinc might have significanteffect on the wound healing of the rats fed normal diets as well as these that given a high fat diet.
Gambaran Histopatologi Insang Ikan Mas di Daerah Endemik Koi Herpesvirus (HISTOPATHOGIC FINDINGS OF GILLS OF THE COMMON CARPS IN THE ENDEMIC AREA OF KOI HERPESVIRUS) Raden Wasito; Hastari Wuryastuti; Bambang Sutrisno
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Koi herpesvirus (KHV)  is the cause of  a lethal disease that affects common carp (Cyprinus carpio)  andkoi (Cyprinus carpio koi). Although, it has been reported that common carps could act as carriers for KHV,their histopathologic findings, especially the gills  have not been identified up to now. In the present study,12  normal, healthy looking common carps including their gills were collected from the endemic area ofKHV in  Sleman, Yogyakarta. All fish were necropsied and the gills were collected and fixed in 10% bufferformalin. Then, the gills were processed histopathologically using routine hematoxyline-eosin stain andexamined under the microscope. Histopathologic examination of the gills exhibited an apparent infiltrationof inflammatory cells, especially lymphocytes. The large oval to polygonal basophilic cells containing largeintranuclear inclusion bodies were also identified. Gills epithelial cells show mass hyperplasia and adhesionwith necrotic changes. Thus, results of this study has led to a reasonable conclusion that KHV infection ispresent in the normal, healthy common carps. One possibility is those KHV  are live viruses (carriers forKHV) and might could act as a source of infection is being discussed.
Identifikasi Koi Herpesvirus dengan Uji Imunopatologi Imunohistokimia Streptavidin Biotin pada Ikan Mas Karier (IDENTIFICATION OF KOI HERPESVIRUS USING IMMUNOPATHOLOGIC IMMUNOHISTOCHEMISTRY OF STREPTAVIDIN BIOTIN IN THE COMMON CARP CARRIERS) Raden Wasito; Hastari Wuryastuti; Bambang Sutrisno
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In  managing the koi herpesvirus (KHV) outbreaks as a routine national program in Indonesia, testingbased on biotechnology, such as  immunopathologic immunohistochemical approach(es) using antibodythat is safe, rapid  and accurate need to be applied. This will hopely assist the Government of Indonesianin improving and enhancing the sustainability of national animal proteins program. The present studywas aimed to develop and apply the immunopathologic immunohistochemistry of streptavidin biotin (IHCSB) for detection of KHV in the apparently normal carps. The gills from 48 common carps  (Cyprinuscarpio) that appear to be healthy were prepared for  DNA-based KHV  by IHC SB.  Common carps werecollected from fish farms which had an outbreak of KHV in 2008-2009 in Yogyakarta.  All fish werenecropsied. The gills were processed histopathologically and then stained for IHC SB with monoclonalantibody anti-KHV. We demonstrated that all of the fish gills were positive for KHV antigen. Thus, it isconcluded that method is useful and consistent, very sensitive and rapid, and is a reliable method to beapplied for field condition to detect antigen KHV in the gills of normal, healthy looking carps.   In addition,and more importantly, the fish can act as a source of KHV (carriers for KHV) and may result in the spreadof diseases among susceptible fish.
Co-Authors Asmarani Kusumawati Charles Rangga Tabbu Devita Anggraeni DEWI RAHMAWATI Dhirgo Adji Ery Sri Rohayati Hastari Wuryastuti Joko Prastowo Kurniasih . Raden Wasito Sitarina Widyarini Sugiyono Sultan Tri Untari Yuli Purwandari Kristiangingrum