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All Journal Jurnal Sain Veteriner Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Jurnal Triton
Wida Wahidah Mubarokah
Politeknik Pembangunan Pertanian Yogyakarta Magelang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Social Sciences Veterinary

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Daya Anthelmintik Infusa Biji Buah Pinang (Areca catechu) Terhadap Cacing Ascaridia galli Secara in Vitro Wida Wahidah Mubarokah; Wisnu Nurcahyo; Kurniasih Kurniasih
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7800.591 KB) | DOI: 10.22146/jsv.40337

Abstract

A. galli infection caused high economic losses and decreased the amount and quality of egg production. The existence of anthelmintic resistance lead to the development of research on alternative treatments for A. galli. This aim of the research was to know LC50 of Areca catechu on infestation of A. galli. In this research A. galli was taken from a chicken slaughterhouse in Terban, Yogyakarta, and was divided into 8 groups for in vitro research. Group I was treated with an 10% infusion of A. catechu; group II was treated with 12.5% infusion of A. catechu; group III was treated with a 15% infusion of A. catechu; group IV was treated with a 17.5% infusion of A. catechu; group V was treated with 20% infusion of A. catechu; group VI was treated with a 22.5% infusion of A. catechu; group VII was treated with 25% A. catechu infusion and group VIII as negative control (0.9% NaCl). A. galli mortality is recorded every one hour until worm mortality is 100%. The results were then analyzed using the Reed and Muench method. Based on the in vitro test the infusion of Areca catechu against Ascaridia galli can cause death in worms with an LC 50 calculation of 21. 18%.
Pengaruh Infusa Biji Buah Pinang (Areca catechu) Terhadap Tingkat Kematian dan Morfometri Cacing Dewasa Ascaridia galli Secara In Vitro Wida Wahidah Mubarokah; Kurniasih Kurniasih; Wisnu Nurcahyo; Joko Prastowo
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.658 KB) | DOI: 10.22146/jsv.43751

Abstract

The study evaluates in vitro effects of Areca catechu crude aqueous extract  as anthelmintics on Ascaridia galli adult mortality rate and morphometry. Naturally infected chickens were collected from slaughtered chickens at local slaughterhouses in Yogyakarta. Chicken’s intestines were carefully examined and transported to the Parasitological Laboratory of Gadjah Mada University, Yogyakarta. Ascaridia galli were collected from intestines (including duodenum, jejunum, and ileum) and put into a petri dish containing 0.62% saline water. There were 60 A. galli in each concentration of Areca catechu crude aqueous extract. Investigation on mortality rate of adult worms at various concentration and observation as well as differences in A. galli morphometry and mortality rate were analyzed using analysis Anova. The figures of the parasites or the parts of the parasites were captured using camera Lucida and then they were measured using both micrometer and curvimeter. The morphology of the parasites was identified to find out their morphometric characteristics.The results of morphometric observation of the A. galli showed that there was a significant difference in the body width and the vulva length of the females and in the body width, the esophageal length and the esophageal width of the males.This research disclosed that Areca catechu crude aqueous extract significantly affected the mortality rate of adult A.galli at various concentration. This research observed that the best concentration to kill the adult worms was 25% Areca catechu crude aqueous extract.
Study of doking molecule flavonoid Coleus amboinicus in TGF-1b receptor and lowering MDA concentration on Cisplatin-induce Wistar Rats Wida Wahidah Mubarokah; Rondius Solfaine; Lailatul Muniroh
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.55685

Abstract

The aims of this study is to evaluate molecular docking of flavonoid Coleus amboinicus (CA) extracts in transforming growth factor-1b and lowering MDA concentration on cisplatin-induced in Wistar rat. Eighteen male Wistar rats (Rattus norvegicus), 3 months of age with a body weight (BW) of 150-200 g, were allocated into three groups, with six animals per group. The control group received aquadest (P0), the treatment group were treated with single doses of cisplatin (5 mg/kg bw., ip) (P1) and  received 100 mg/kg bw of the CA extracts (P2) respectively for 7 days. Bloods collected for analysis of serum alkaline phospatase (AP), Blood Nitrogen Urea (BUN) and Malondialdehid (MDA) concentrations. The levels of Malondialdehid (MDA) concentrations were analysed by Avidin-Horseradish Peroxidase (HRP) Sandwich-ELISA. All groups were sacrified for histopathology. Coleus amboinicus extract significantly decreased the level of AP, BUN and MDA concentrations compared to the control group (p<0.05). The level of MDA could be detected by its level significantly decreased in CA treatment group (p<0.05). Coleus amboinicus (CA) extract has a flavonoid as a marker compound of CA extract has stronger bind to the TGF-β1receptor than its of 3WA_601 ligand in silico analyzed. In histopathological examination showed that cisplatin-induced could alter severe multifocal hemorrhage, interstitial congestion, cell inflammatory, acute glomerular and tubular injury with necrotic cells. Immunohistochemical staining labeled with TGF-1β monoclonal antibodies (Mab) showed marked expression of brownish color aggregates on the surface of tubular epithelial cells and around glomerular mesangial cells in the CA treatment group. This study was concluded that CA extract is inhibited renal tissue injuries by lowering MDA and increasing TGF-1b expression on cisplatin-induced rats. Flavonoid as marker of CA extract has stronger bind to TGF-1b receptor by in silico.
Daya Antelmintik Serbuk Kulit Nanas (Ananas comosus) terhadap Cacing Haemochus contortus pada Domba Dewi Pranatasari; Rido Florensius Manik; Budi Purwo Widiarso; Wida Wahidah Mubarokah
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.66420

Abstract

The problem facing sheep breeders in breeding sheep was digestive tract parasite of worm (nematodiasis and haemonchosis). Resistance to anthelmintics was the reason for the study of alternative medication of H. contortus infection. It aimed at finding out the effectiveness of the application of pineapple peel powder as H. contortus anthelmintic in sheep and the dose of the pineapple peel powder as the H. contortus anthelmintic. It used 15 sheep that were assigned to 5 groups. Group I served as positive control with the application of albendazole (Kalbazen) anthelmintic, Group II was treated using the pineapple peel powder at the dose of 150 mg/kg BW. Group III was treated using the pineapple peel powder at the dose of 200 mg/kg BW. Group IV was treated using the pineapple peel powder at the dose of 250 mg/kg BW. And, Group V served as negative control without any treatment. The treatments were conducted for 14 days and the resulting statistic data were analyzed using comparative descriptive method by comparing initial data (before the treatments) and final data (after the treatments). The comparative data showed that there was significant change in the observed variables. The results of the study showed that the pineapple peel powder could be used as the anthelmintic of the H. contortus in the sheep and the dose of 250 mg/kg BW most significantly decreased the mean number of the eggs of the worm per gram of feces
THE ANTHELMINTIC POTENTIAL OF TURMERIC (Curcuma domestica) EXTRACT AGAINST Ascaridia galli WORMS IN CHICKENS In Vivo Yudiani Rina Kusuma; Zahid Fahmi Dai; sunardi sunardi; wida wahidah mubarokah
Jurnal Sain Veteriner Vol 40, No 1 (2022): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.67911

Abstract

Ascaridia galli worm was chicken parasite that inflicted significant economic loss for breeders. Expensive commercial anthelmintic drug and drug resistance were the reasons why herbal anthelmintic study became strategic. The study aimed at finding out the effect of the application of turmeric (Curcuma domestica) extract in vivo on the number of eggs per gram (EPG) in feces. It used 30 free ranging chickens that were classified into 2 groups of 5 chickens with 3 repetitions. Group I served as control (Aquadestilata) and group II was treated using turmeric extract at the concentration of 2%, 3 times a week. Observation was conducted on days 1, 7, 14, 21, and 28. The results were then analyzed using Anova and advanced LSD test. The results of in vivo test showed that the application of the turmeric significantly decreased the number of the EPG as compared to negative control. The application of the turmeric had anthelmintic potential and significant effect on the decrease in the number of the EPG.
Respon Peternak terhadap Pencegahan dan Pengobatan Penyakit Cacing Gastrointestinal Pada Kambing di Desa Klopo Kecamatan Tegalrejo Kabupaten Magelang: Farmers Response on Prevention and Treatment Gastrointestine Worm Disease in Goat in Klopo Village Tegalrejo District Magelang Sub-Province Budi Purwo Widiarso; Wida Wahidah Mubarokah
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 9 No. 2 (2019): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Vet
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/jipvet.v9i2.68

Abstract

This study was conducted to determine the response of farmers to the prevention and treatment gastrointestine worm disease in goats and know factors that influencing farmer response Tools and materials used are stationery, questioner, folder, worm drug and vitamin of B complex. The respondents were 30 people. Data was taken from pre-test and post-test by conducting direct interview regarding prevention and treatment gastrointestine worm disease in goat by using questioner. Based on the regression analysis obtained by multiple linear equations as follows: Y = 28.946 + 5.084X1 - 0,215X2 + 0,171X3+ 0,548X4 + e. From model of regresion above, it can be intepreted that each increase in the vallue of X1 (education) equal to 1 % will add respon equal to 5.084, each increase in the value of X2 (age) by 1 % will reduce the respon by 0.215, each increase of value of X3 (experience of farming) by 1 % will add value of respon equal to 0.171, and each increase of value of X4 (amount of the ownership of livestock) by 1 % will add value of 0.548. This study concluded that there was an increase in knowledge aspects of 14.87 with EP 82.6% and EPP 77.36%, attitude aspects amounting to 10.47 with EP 90.88% and EPP 82.12% and skills aspects of 5.1 with EP 86.6% and EPP 79.19% and an increase in response value from aspects of knowledge, attitudes, and skills of 30.39%, namely from a low response to a high response to the prevention and treatment of gastrointestinal worm disease in goats. Education is the most significant factor that has a very significant effect of (P 1 ≤0.01) on the farmer response average. Keywords: prevention, treatment, gastrointestine worm, goat, response
Uji Kualitas Fisik dan Mikroskopis (pH, Kadar Air dan Jumlah Total Mikroba) Daging Broiler di Kabupaten Jember Hariadi Subagja; Dini Aprilia; Agus Hadi Prayitno; Anang Febri Prasetyo; Wida Wahidah Mubarokah
JURNAL TRITON Vol 13 No 1 (2022): JURNAL TRITON
Publisher : Politeknik Pembangunan Pertanian Manokwari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47687/jt.v13i1.237

Abstract

Daging broiler adalah salah satu produk pangan asal peternakan yang banyak diminati masyarakat karena merupakan sumber protein hewani yang mengandung asam amino esensial dan nilai gizi yang baik bagi tubuh manusia. Penelitian ini bertujuan menganalisis tingkat cemaran bakteri daging broiler yang dijual pada Pasar Tradisional dan Modern di Kabupaten Jember. Sebanyak 30 sampel daging broiler diambil secara acak (random sampling) dari 3 pasar tradisional dan 3 pasar modern di Kabupaten Jember. Data yang diperoleh dianalisis secara deskriptif. Parameter yang diamati yaitu nilai pH, kadar air, dan total mikroba daging. Data yang diperoleh dianalisis dengan menggunakan independent sample t test dan dianalisis secara deskriptif dengan kondisi di lapangan. Hasil analisis dari penelitian ini menunjukkan bahwa daging yang diperoleh dari pasar tradisional memiliki rata-rata nilai pH 5,79, sedangkan daging dari pasar modrn memiliki rata-rata nilai pH 5,96. Daging dari pasar tradisional memiliki kadar air 76,40%, sedangkan kadar air daging dari pasar modern yaitu 75,21%. TPC daging broiler dari pasar tradisional lebih tinggi dari pasar moder. Jumlah TPC daging dari pasar tradisional yaitu sebesar 6,055 x 107 dan jumlah TPC daging pada pasar modern yaitu 2,636 x 105.Kesimpulan dari penelitian ini yaitu daging broiler yang diperoleh dari pasar modern memiliki nilai TPC yang telah sesusai dengan SNI (3924:2009),sedangkan daging dari pasar tradisional memiliki nilai TPC melebihi standar maksimum yang telah ditentukan SNI. Tingginya jumlah TPC daging pada pasar tradisional menyebabkan daging dari pasar tersebut memiliki kualitas yang kurang baik karena tingginya jumlah mikroba dalam daging yang menyebabkan daging lebih cepat rusak.
In Vitro Anthelmintic Areca catechu Crude Aqueous Extract Against Haemonchus contortus in The Sheep Annisa Putri Cahyani; Budi Purwo Widiarso; Supriyanto -; Wida Wahidah Mubarokah
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70399

Abstract

Haemonchosis is a gastrointestinal parasitic disease in sheep caused by Haemonchus contortus. The biggest economic losses due to this disease are mortality, decreased production, stunted growth, and low body weight. The existence of resistance to synthetic anthelmintics causes the development of research on alternative treatments to herbal anthelmintics to be a strategic step. This study aims to analyze the effect of Areca catechu on Haemonchus contortus in vitro so that the Lethal Concentration 50 (LC 50) can be determined. This study was divided into 9 groups. Group I was treated with 2.5% A. catechu crude aqueous extract; group II was treated with 5% of A. catechu crude aqueous extract; group III was treated with 7.5% A. catechu crude aqueous extract; group IV was treated with 10% of A. catechu crude aqueous extract; group V was treated with 12.5% A. catechu crude aqueous extract; group VI was treated with 15% A. catechu crude aqueous extract; Group VII was treated with 17.5% A. catechu crude aqueous extract, group VIII was a negative control (0.9% NaCl) and group IX was a positive control (Albendazole). The mortality of H. contortus was recorded every hour until the worm mortality was 100%. The results were then analyzed using the Reed and Muench method. Based on the in vitro test of Areca catechu crude aqueous extract against H.contortus it can cause mortality in worms with an Lethal Concentration 50 (LC 50) calculation result of 11.11%.
Co-Authors Agus Hadi Prayitno Anang Febri Prasetyo, Anang Febri Annisa Putri Cahyani Budi Purwo Widiarso Budi Purwo Widiarso Dewi Pranatasari Dini Aprilia Hariadi Subagja Joko Prastowo Kurniasih Kurniasih Kurniasih Kurniasih Lailatul Muniroh Raden Wisnu Nurcahyo Rido Florensius Manik Rondius Solfaine sunardi sunardi Supriyanto - Yudiani Rina Kusuma Zahid Fahmi Dai